LOCUS       Exported                4439 bp ds-DNA     circular SYN 04-SEP-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pJet1.2 kanR resistance cassette
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Frank
  TITLE     Direct Submission
  JOURNAL   Exported Sep 4, 2018 from SnapGene 4.2.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="synthetic DNA construct"
                     /note="/dnas_title=pSG0723 pJET1.2 kanR cassette"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    385..388
                     /label=Rup
                     /note="Rup"
     CDS             920..1714
                     /codon_start=1
                     /label=kananamycin resistance cassette
                     /label=kanR
                     /note="unnamed protein product; kanamycin resistance gene"
                     /note="/dnas_title=kanR"
                     /note="/vntifkey=4"
                     /db_xref="GI:47034"
                     /db_xref="GOA:P0A3Y5"
                     /db_xref="InterPro:IPR002575"
                     /db_xref="InterPro:IPR011009"
                     /db_xref="PDB:1J7I"
                     /db_xref="PDB:1J7L"
                     /db_xref="PDB:1J7U"
                     /db_xref="PDB:1L8T"
                     /db_xref="PDB:2B0Q"
                     /db_xref="PDB:2BKK"
                     /db_xref="PDB:3H8P"
                     /db_xref="UniProtKB/Swiss-Prot:P0A3Y5"
                     /protein_id="CAA24789.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      1776..1818
                     /label=ter
                     /note="/dnas_title=ter"
                     /note="/vntifkey=43"
     misc_feature    1820..1823
                     /label=Rdo
                     /note="Rdo"
     promoter        complement(2271..2301)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    2316..2337
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2628..3216)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3387..4247)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4248..4352)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tgttacaggt ctcgaataaa acccagcgaa ccatttgagg tgataggtaa
      421 gattataccg aggtatgaaa acgagaattg gacctttaca gaattactct atgaagcgcc
      481 atatttaaaa agctaccaag acgaagagga tgaagaggat gaggaggcag attgccttga
      541 atatattgac aatactgata agataatata tcttttatat agaagatatc gccgtatgta
      601 aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata gaatgggcaa
      661 agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt atagcttgta
      721 aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact
      781 ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca
      841 gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa
      901 aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat
      961 cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct
     1021 ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac
     1081 ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc
     1141 aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga
     1201 tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct
     1261 gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa
     1321 tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt
     1381 ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt
     1441 tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag
     1501 caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag
     1561 ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga
     1621 agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat
     1681 aaaatattat attttactgg atgaattgtt ttagcattat tcaaaggtga aagcactcgt
     1741 taagtatatg aaaaattcta tagaaacttc tctcaattaa gaaaaaggcc ccaacatctt
     1801 ggggcctttt tcttttttat ctacgagacc caatgaatct ttctagaaga tctcctacaa
     1861 tattctcagc tgccatggaa aatcgatgtt cttcttttat tctctcaaga ttttcaggct
     1921 gtatattaaa acttatatta agaactatgc taaccacctc atcaggaacc gttgtaggtg
     1981 gcgtgggttt tcttggcaat cgactctcat gaaaactacg agctaaatat tcaatatgtt
     2041 cctcttgacc aactttattc tgcatttttt ttgaacgagg tttagagcaa gcttcaggaa
     2101 actgagacag gaattttatt aaaaatttaa attttgaaga aagttcaggg ttaatagcat
     2161 ccattttttg ctttgcaagt tcctcagcat tcttaacaaa agacgtctct tttgacatgt
     2221 ttaaagttta aacctcctgt gtgaaattat tatccgctca taattccaca cattatacga
     2281 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     2341 gcgttgcgct cactgccaat tgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     2401 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2461 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2521 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2581 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2641 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2701 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2761 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2821 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2881 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2941 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3001 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3061 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3121 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3181 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3241 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3301 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3361 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3421 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3481 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3541 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3601 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3661 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3721 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3781 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3841 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3901 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3961 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4021 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4081 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4141 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4201 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4261 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4321 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4381 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcc
//
