LOCUS       Exported                3214 bp ds-DNA     circular SYN 04-SEP-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pJet1.2 HA6-tag
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Frank
  TITLE     Direct Submission
  JOURNAL   Exported Sep 4, 2018 from SnapGene 4.2.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    385..388
                     /label=RtagC
                     /note="/vntifkey=21"
     CDS             complement(395..484)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             complement(491..580)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     misc_feature    595..598
                     /label=RtagN
                     /note="/vntifkey=21"
     promoter        complement(1046..1076)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1091..1112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1403..1991)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2162..3022)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3023..3127)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga ttcattgggt ctcgcttagc tagaagcgta atctggaacg tcatatggat
      421 aggatcctgc atagtccggg acgtcatagg gatagcccgc atagtcagga acatcgtatg
      481 ggtatctaga agcgtaatct ggaacgtcat atggatagga tcctgcatag tccgggacgt
      541 catagggata gcccgcatag tcaggaacat cgtatgggta tctagcagca gaaccgcttg
      601 agacctgtaa catctttcta gaagatctcc tacaatattc tcagctgcca tggaaaatcg
      661 atgttcttct tttattctct caagattttc aggctgtata ttaaaactta tattaagaac
      721 tatgctaacc acctcatcag gaaccgttgt aggtggcgtg ggttttcttg gcaatcgact
      781 ctcatgaaaa ctacgagcta aatattcaat atgttcctct tgaccaactt tattctgcat
      841 tttttttgaa cgaggtttag agcaagcttc aggaaactga gacaggaatt ttattaaaaa
      901 tttaaatttt gaagaaagtt cagggttaat agcatccatt ttttgctttg caagttcctc
      961 agcattctta acaaaagacg tctcttttga catgtttaaa gtttaaacct cctgtgtgaa
     1021 attattatcc gctcataatt ccacacatta tacgagccgg aagcataaag tgtaaagcct
     1081 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg ccaattgctt
     1141 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     1201 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1261 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     1321 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1381 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1441 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1501 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1561 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1621 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1681 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1741 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1801 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     1861 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1921 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1981 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2041 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2101 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2161 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2221 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2281 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     2341 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     2401 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     2461 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     2521 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     2581 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     2641 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     2701 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     2761 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     2821 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2881 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2941 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3001 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3061 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     3121 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     3181 cattaaccta taaaaatagg cgtatcacga ggcc
//
