LOCUS Exported 3873 bp ds-DNA circular SYN 04-SEP-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pJet1.2 sivC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3873) AUTHORS Frank TITLE Direct Submission JOURNAL Exported Sep 4, 2018 from SnapGene 4.2.2 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3873 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 305..323 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 379..382 /label=RD /note="/vntifkey=21" CDS 704..1215 /codon_start=1 /label=sivC /label=YlqB /note="/vntifkey=4" /translation="MNPILRRIFMKKIGLLFMLCLAALFTIGFPAQQADAAEAPYKASI TNISTDGGVYGKINYGQGQYWRVKYNITVSGKLLDQNGQPVPNAPVRFEADTKVGNTTQ TASGTTDANGTFEVPMYLGPAAGYYTYYTSVSVHYYDIIPFRVFSGESRLVSTDNSLYH FAYQVRR" terminator 1215..1257 /label=ter+ /note="/vntifkey=43" terminator 1216..1259 /label=ter- /note="/vntifkey=43" misc_feature 1260..1263 /label=Rdo /note="/vntifkey=21" promoter complement(1705..1735) /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" protein_bind 1750..1771 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(2062..2650) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2821..3681) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3682..3786) /gene="bla" /label=AmpR promoter ORIGIN 1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc 61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc 121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact 181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt 241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc 301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt 361 ttcagcaaga tggtctctat ggccaagctt gcatgctaaa aaagaaaaag gcccgaagat 421 gttcgggcct ttttcttacc tagggagaaa tcgtccaaat aataaagcga catatagctc 481 ttctgttttg acgtcaacaa cgactgataa aaatcaaaca gataattcat tctcgttgtc 541 ttttcgagtg acatttgagc tctccccttt gttaagtcaa atccctttac gataacttag 601 tttacacggt tttaatgaca ctgtcaagtt tttatcttgt caaagcggcg attaactttt 661 ccaaaatacg ctaaatatca tccaatttat tccgatattg tatatgaacc ctattttaag 721 gaggattttt atgaaaaaaa tcggtttatt gtttatgtta tgtctggcag ccctgtttac 781 aataggtttc ccggcacagc aagcagacgc tgctgaggca ccttataagg cctcaatcac 841 aaatatcagc acagatggag gggtatacgg taaaatcaat tacggacaag gtcaatattg 901 gcgagtcaag tacaatatta cagtaagcgg caaattactc gaccaaaacg gacagccagt 961 gccaaacgcg cccgtaagat ttgaagcgga cacgaaggtc ggaaacacaa cacaaactgc 1021 ttcaggaacg actgatgcaa acggaacatt tgaggttcca atgtatttgg ggccagctgc 1081 tggttactat acgtattaca catctgtttc cgttcattac tatgacatca tcccattcag 1141 agttttttct ggtgagtcaa gactggtatc cactgataac agcctttatc atttcgccta 1201 tcaagtaaga agataaaaaa gaaaaaggcc ccaagatgtt ggggcctttt tcttaatcgt 1261 agatgagacc atctttctag aagatctcct acaatattct cagctgccat ggaaaatcga 1321 tgttcttctt ttattctctc aagattttca ggctgtatat taaaacttat attaagaact 1381 atgctaacca cctcatcagg aaccgttgta ggtggcgtgg gttttcttgg caatcgactc 1441 tcatgaaaac tacgagctaa atattcaata tgttcctctt gaccaacttt attctgcatt 1501 ttttttgaac gaggtttaga gcaagcttca ggaaactgag acaggaattt tattaaaaat 1561 ttaaattttg aagaaagttc agggttaata gcatccattt tttgctttgc aagttcctca 1621 gcattcttaa caaaagacgt ctcttttgac atgtttaaag tttaaacctc ctgtgtgaaa 1681 ttattatccg ctcataattc cacacattat acgagccgga agcataaagt gtaaagcctg 1741 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc caattgcttt 1801 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 1861 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 1921 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 1981 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 2041 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 2101 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 2161 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 2221 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 2281 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 2341 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 2401 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 2461 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 2521 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 2581 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 2641 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 2701 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 2761 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 2821 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 2881 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 2941 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 3001 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 3061 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 3121 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 3181 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 3241 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 3301 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 3361 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 3421 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 3481 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 3541 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 3601 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 3661 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 3721 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 3781 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 3841 attaacctat aaaaataggc gtatcacgag gcc //