LOCUS       Exported                9629 bp ds-DNA     circular SYN 29-JUN-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 4, 2018 from SnapGene Viewer 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    659..1158
                     /label=CMV promoter
     misc_feature    1176..1356
                     /label=HIV-1_5_LTR
     misc_feature    1178..1274
                     /label=R
     misc_feature    1275..1356
                     /label=U5
     misc_feature    1467..1511
                     /label=HIV-1_psi_pack
     misc_feature    2027..2260
                     /label=RRE
     misc_feature    2791..2806
                     /label=cPPT
     misc_feature    2956..3341
                     /label=pKLV2-EF1a
     misc_feature    2962..3227
                     /label=U6
     misc_feature    3228..3248
                     /label=U6-gRNA5_BbsI-IDT
     misc_feature    3249..3334
                     /label=iScaffold
     misc_feature    3348..4947
                     /label=EF1a
     CDS             4966..5720
                     /codon_start=1
                     /label=open reading frame#1
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLNGGGSGGEGRGSLLTCGD"
     CDS             4966..5667
                     /codon_start=1
                     /label=BFP
                     /translation="MGSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL
                     KLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY
                     CDLPSKLGHKLN"
     CDS             5668..5739
                     /codon_start=1
                     /label=T2A-2
                     /translation="GGGSGGEGRGSLLTCGDVEENPGP"
     CDS             5746..6141
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             6142..6213
                     /codon_start=1
                     /label=T2A-2
                     /translation="GGGSGGEGRGSLLTCGDVEENPGP"
     CDS             6142..6194
                     /codon_start=1
                     /label=open reading frame#1
                     /translation="GGGSGGEGRGSLLTCGD"
     misc_feature    6247..6845
                     /label=pKLV2.W-
     misc_feature    6247..6845
                     /label=pKLV3
     misc_feature    6253..6840
                     /label=WPRE
     misc_feature    6846..7417
                     /label=0. pBS-MCS
     misc_feature    6895..6910
                     /label=cPPT
     misc_feature    6911..6986
                     /label=DU3
     misc_feature    6987..7083
                     /label=R
     misc_feature    7084..7165
                     /label=U5
     misc_feature    7188..7415
                     /label=bpA
     CDS             complement(8644..9501)
                     /codon_start=1
                     /label=amp
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgactagtac
      661 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      721 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      781 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      841 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      901 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      961 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     1021 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     1081 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     1141 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
     1201 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     1261 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     1321 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     1381 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     1441 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     1501 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1561 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1621 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1681 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1741 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1801 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1861 gcaagcggcc ggccgcgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1921 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1981 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     2041 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     2101 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     2161 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     2221 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     2281 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     2341 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     2401 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     2461 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     2521 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2581 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2641 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2701 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     2761 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     2821 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     2881 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     2941 ttagtaccgg gccctacgcg tccaaggtcg ggcaggaaga gggcctattt cccatgattc
     3001 cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg
     3061 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
     3121 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
     3181 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgga gacgcatgac
     3241 tcgtctcagt ttaagagcta tgctggaaac agcatagcaa gtttaaataa ggctagtccg
     3301 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tacgcgtgag gggcgcggaa
     3361 gccggggcgg aggaggagcc aaccccgggc tgggctgaga cccgcagagg aagacgctct
     3421 agggatttgt cccggactag cgagatggca aggctgagga cgggaggctg attgagaggc
     3481 gaaggtacac cctaatctca atacaacctt tggagctaag ccagcaatgg tagagggaag
     3541 attctgcacg tcccttccag gcggcctccc cgtcaccacc ccccccaacc cgccccgacc
     3601 ggagctgaga gtaattcata caaaaggact cgcccctgcc ttggggaatc ccagggaccg
     3661 tcgttaaact cccactaacg tagaacccag agatcgctgc gttcccgccc cctcacccgc
     3721 ccgctctcgt catcactgag gtggagaaga gcatgcgtga ggctccggtg cccgtcagtg
     3781 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     3841 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     3901 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     3961 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     4021 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacgc ccctggctgc
     4081 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     4141 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcttggg cgctggggcc
     4201 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     4261 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     4321 atgcgggcca agatcgatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     4381 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     4441 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     4501 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     4561 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
     4621 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
     4681 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
     4741 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     4801 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     4861 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     4921 ttttttcttc catttcaggt gtcgtgggaa gcttggcgcg ccaccatggg cagcgagctg
     4981 attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc
     5041 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
     5101 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac
     5161 ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc
     5221 cctgagggct tcacatggga gagagtcacc acatacgagg acgggggcgt gctgaccgct
     5281 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
     5341 aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc
     5401 gagaccctgt accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc
     5461 gtgggcggga gccatctgat cgcaaacatc aagaccacat atagatccaa gaaacccgct
     5521 aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag
     5581 gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc
     5641 cctagcaaac tggggcacaa gcttaatgga gggggcagcg gcggggaagg aaggggctcc
     5701 ctgctcacct gcggcgacgt ggaagagaac cccggacctg tcgacatggc caagcctttg
     5761 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
     5821 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
     5881 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
     5941 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
     6001 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
     6061 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
     6121 tctggttatg tgtgggaggg cggagggggc agcggcgggg aaggaagggg ctccctgctc
     6181 acctgcggcg acgtggaaga gaaccccgga cctatgcatt gatgataatc tagaggatcc
     6241 gctagcggcc gctcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6301 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     6361 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     6421 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6481 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     6541 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6601 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     6661 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6721 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6781 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     6841 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6901 aaagggggga ctggaagggc taattcactc ccaaagaagt caagatctgc tttttgcctg
     6961 tactgggtct ctctggttag accagagtct ctctggttag accagatctg agcctgggag
     7021 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7081 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7141 tagtcagtgt ggaaaatctc tagcagttta aacccgctga tcagcctcga ctgtgccttc
     7201 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     7261 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     7321 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagtcaa
     7381 tagcaggcat gctggggatg cggtgggctc tatgggcggc cgttaattaa gcttttgttc
     7441 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7501 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7561 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7621 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7681 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7741 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7801 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7861 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7921 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7981 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8041 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8101 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8161 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8221 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8281 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     8341 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8401 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8461 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8521 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8581 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8641 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8701 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8761 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8821 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8881 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8941 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9001 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9061 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9121 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9181 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9241 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9301 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9361 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9421 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9481 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9541 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9601 tccgcgcaca tttccccgaa aagtgccac
//
