LOCUS       pHIV7CMVCreMyc2AEGFP        8781 bp    DNA     circular     27-AUG-2018
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-11291991|
COMMENT     VNTDBDATE|-12439569|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pHIV7CMVCreMyc2AEGFP|
COMMENT     VNTAUTHORNAME|Bioinformatics Core|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                            2 4 "ATCGAT" "TAGCTA") 
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COMMENT                            3 3 "TTTAAA" "AAATTT") 
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COMMENT                                            " N1: 2629 (on complementary strand)" 
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COMMENT                                            " N2: 7977 (on complementary strand)" 
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COMMENT                            1 5 "CCATGG" "GGTACC") 
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COMMENT                            4 2 "CGATCG" "GCTAGC") 
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COMMENT                      #197=(CRSFolderPar 
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COMMENT                            3 3 "AGTACT" "TCATGA") 
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COMMENT                            3 3 "CCCGGG" "GGGCCC") 
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COMMENT                            "CCANNNNNNNNNTGG" "GGTNNNNNNNNNACC") 
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COMMENT                            1 5 "CCCGGG" "GGGCCC") 
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COMMENT                            5 5 "GAANNNNTTC" "CTTNNNNAAG"))) 
COMMENT                    "Restriction/Methylation Map:  (no more than 3 sites)")))) 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Bam}HI (6169)" "@N (@S)" 5 -15.0088 
COMMENT                                   -12.1963 0 -10 4.77049 0.799245 #359#) 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Sna}BI (6527)" "@N (@S)" 5 -14.6134 
COMMENT                                   -1.74233 0 -10 4.22101 0.799245 #366#) 
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COMMENT                                    "Georgia") 1.74233 0.555556 0 
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COMMENT                                   13.9386 0 -10 4.39585 0.799245 #368#) 
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FEATURES             Location/Qualifiers
     misc_feature    6180..6748
                     /vntifkey="21"
                     /label=CMV\Promoter
     CDS             6934..7962
                     /vntifkey="4"
                     /label=Cre
     insertion_seq   7963..7992
                     /vntifkey="14"
                     /label=Myc
     misc_feature    8065..8781
                     /vntifkey="21"
                     /label=EGFP\Coding\region,\No\start\codon
     misc_feature    7999..8064
                     /vntifkey="21"
                     /label=2A
     misc_feature    2535..2639
                     /vntifkey="21"
                     /label=AmpR\promoter
     misc_feature    2640..3500
                     /vntifkey="21"
                     /label=AmpR
BASE COUNT      915 a       910 c       926 g       829 t      5201 others
ORIGIN
        1 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
       61 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     2521 nnnnnnnnnn nnnncgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 
     2581 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 
     2641 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 
     2701 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 
     2761 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 
     2821 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 
     2881 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 
     2941 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 
     3001 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 
     3061 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 
     3121 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 
     3181 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 
     3241 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 
     3301 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 
     3361 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 
     3421 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 
     3481 cactgattaa gcattggtaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngga tcccccggga 
     6181 cattgattat tgataatagt aatcaattac ggggtcatta gttcatagcc catatatgga 
     6241 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 
     6301 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 
     6361 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 
     6421 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 
     6481 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 
     6541 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 
     6601 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 
     6661 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 
     6721 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta 
     6781 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc 
     6841 tggttattgt gctgtctcat cattttggca aagaatttcg aggggcagag ccgatcctgt 
     6901 acactttact taaaaccatt atctgagtgt gaaatgtcca atttactgac cgtacaccaa 
     6961 aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac 
     7021 atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct gtccgtttgc 
     7081 cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc agaacctgaa 
     7141 gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa aactatccag 
     7201 caacatttgg gccagctaaa catgcttcat cgtcggtccg ggctgccacg accaagtgac 
     7261 agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa 
     7321 cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg ttcactcatg 
     7381 gaaaatagcg atcgctgcca ggatatacgt aatctggcat ttctggggat tgcttataac 
     7441 accctgttac gtatagccga aattgccagg atcagggtta aagatatctc acgtactgac 
     7501 ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac cgcaggtgta 
     7561 gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc cgtctctggt 
     7621 gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg 
     7681 ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga agcaactcat 
     7741 cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg gtctggacac 
     7801 agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat accggagatc 
     7861 atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg taacctggat 
     7921 agtgaaacag gggcaatggt gcgcctgctg gaagatggcg atgaacaaaa actcatctca 
     7981 gaagaggatc tggaattcgg atccggagcc acgaacttct ctctgttaaa gcaagcagga 
     8041 gacgtggaag aaaaccccgg tcctgtgagc aagggcgagg agctgttcac cggggtggtg 
     8101 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 
     8161 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 
     8221 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc 
     8281 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac 
     8341 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 
     8401 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 
     8461 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 
     8521 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 
     8581 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 
     8641 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac 
     8701 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 
     8761 atggacgagc tgtacaagta a 
//
