LOCUS       Exported               13097 bp ds-DNA     circular SYN 04-SEP-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13097)
  AUTHORS   BLO
  TITLE     Direct Submission
  JOURNAL   Exported Sep 5, 2018 from SnapGene 4.2.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13097
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1807..1924
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1953..2193
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2202..2313
                     /label=SpyM2-sgRNA (BsmBI)
     misc_feature    2314..2320
                     /label=U6 terminator
     promoter        2358..2588
                     /label=EFS
     CDS             2610..2675
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2682..2702
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2727..6827
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6828..6875
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6897..6950
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6951..7658
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             7674..7730
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7731..8756
                     /codon_start=1
                     /label=Hygro
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    8792..9380
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element (to increase levels of transcripts)"
     LTR             9587..9820
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9892..10013
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      10053..10188
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      10425..10880
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        10909..11013
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11014..11874
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      12045..12633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacaataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     1921 ttttatcgat aacgagacta gcctcgatcg ccgagggcct atttcccatg attccttcat
     1981 atttgcatat acgatacaag gctgttagag agataattag aattaatttg actgtaaaca
     2041 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     2101 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     2161 atttcttggc tttatatatc ttgtggaaag gacgaaacac cggagacgga ggacgacgaa
     2221 cgtctctgtt taagagctat gctggaaaca gcatagcaag tttaaataag gctagtccgt
     2281 tatcaacttg aaaaagtggc accgagtcgg tgcttttttt gaattcgcta gctaggtctt
     2341 gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     2401 ccccgagaag ttggggggag gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg
     2461 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     2521 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     2581 aacacaggac cggtagcgct cccgggatgg actataagga ccacgacgga gactacaagg
     2641 atcatgatat tgattacaaa gacgatgacg ataagatggc cccaaagaag aagcggaagg
     2701 tcggtatcca cggagtccca gcagccgaca agaagtacag catcggcctg gacatcggca
     2761 ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca
     2821 aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt
     2881 tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca
     2941 ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg
     3001 tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc
     3061 acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc
     3121 ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc
     3181 tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg
     3241 acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca
     3301 accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt
     3361 ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg cccggcgaga
     3421 agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca
     3481 agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg
     3541 acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg
     3601 ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca
     3661 ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac caggacctga
     3721 ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg
     3781 accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct
     3841 acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc
     3901 tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc atcccccacc
     3961 agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt tacccattcc
     4021 tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc tactacgtgg
     4081 gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc gaggaaacca
     4141 tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag agcttcatcg
     4201 agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc
     4261 tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg
     4321 gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt
     4381 tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg
     4441 agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca
     4501 cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg
     4561 aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg
     4621 aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc
     4681 ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca
     4741 agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact
     4801 tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg
     4861 tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca
     4921 ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc
     4981 ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg
     5041 gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca
     5101 gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc
     5161 tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc
     5221 tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac gactccatcg
     5281 acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg
     5341 aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta
     5401 cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata
     5461 aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac
     5521 agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag
     5581 tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt
     5641 acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg
     5701 tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact
     5761 acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta
     5821 ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg
     5881 ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg
     5941 tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga
     6001 atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca
     6061 agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg
     6121 gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca
     6181 agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca
     6241 gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg
     6301 acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa
     6361 tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc tccaaatatg
     6421 tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg
     6481 agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga
     6541 tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg
     6601 cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt
     6661 ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc
     6721 ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca
     6781 ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggcgacaaa aggccggcgg
     6841 ccacgaaaaa ggccggccag gcaaaaaaga aaaagggatc cgaatccggc agtggagagg
     6901 gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggccca atggtgagca
     6961 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     7021 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     7081 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     7141 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     7201 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     7261 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     7321 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     7381 aaaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     7441 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     7501 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     7561 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     7621 gccgccactc caccggcggc atggacgagc tgtacaagcc cgggggaagc ggagctacta
     7681 acttcagcct gctgaagcag gctggagatg tggaggagaa ccctggacct atgaaaaagc
     7741 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtatccg
     7801 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     7861 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     7921 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggagttca
     7981 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     8041 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggcaatggat gcgatcgctg
     8101 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     8161 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     8221 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     8281 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     8341 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     8401 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     8461 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     8521 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     8581 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     8641 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     8701 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaataggaat
     8761 tctaatgtcg acttcgaaac gcgtctggaa caatcaacct ctggattaca aaatttgtga
     8821 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     8881 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     8941 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     9001 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     9061 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     9121 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     9181 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     9241 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     9301 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     9361 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     9421 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     9481 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     9541 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     9601 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     9661 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     9721 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     9781 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     9841 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     9901 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     9961 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    10021 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
    10081 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    10141 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
    10201 caattcgctt ttgcgtcgaa acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
    10261 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
    10321 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
    10381 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg
    10441 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
    10501 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
    10561 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
    10621 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
    10681 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
    10741 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
    10801 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
    10861 aatattaacg tttacaattt cccaggtggc acttttcggg gaaatgtgcg cggaacccct
    10921 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
    10981 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
    11041 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
    11101 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
    11161 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
    11221 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
    11281 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
    11341 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
    11401 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
    11461 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
    11521 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
    11581 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
    11641 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
    11701 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    11761 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    11821 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    11881 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    11941 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    12001 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    12061 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    12121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    12181 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    12241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    12301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    12361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    12421 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    12481 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    12541 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    12601 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    12661 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    12721 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    12781 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    12841 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
    12901 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
    12961 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
    13021 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
    13081 aagctggagc tgcaagc
//
