LOCUS       Exported                7218 bp ds-DNA     circular SYN 06-SEP-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   HMS Neurobiology
  TITLE     Direct Submission
  JOURNAL   Exported Sep 6, 2018 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     promoter        242..1419
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          473..1410
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      1439..1448
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1445..1987
                     /codon_start=1
                     /label=SYP
                     /translation="MDVVNQLVAGGQFRVVKEPLGFVKVLQWVFAIFAFATCGSYTGEL
                     RLSVECANKTESALNIEVEFEYPFRLHQVYFDAPSCVKGGTTKIFLVGDYSSSAEFFVT
                     VAVFAFLYSMGALATYIFLQNKYRENNKGPMMDFLATAVFAFMWLVSSSAWAKGLSDVK
                     MATDPENIIKEMPMCRQT"
     CDS             2006..2929
                     /codon_start=1
                     /product="synthetic horseradish peroxidase isoenzyme C 
                     (HRP-C) subunit alpha-1 (E.C. 1.11.1.7) (Smith et al., 
                     1990)"
                     /label=HRP
                     /translation="QLTPTFYDNSCPNVSNIVRDTIVNELRSDPRIAASILRLHFHDCF
                     VNGCDASILLDNTTSFRTEKDAFGNANSARGFPVIDRMKAAVESACPRTVSCADLLTIA
                     AQQSVTLAGGPSWRVPLGRRDSLQAFLDLANANLPAPFFTLPQLKDSFRNVGLNRSSDL
                     VALSGGHTFGKNQCRFIMDRLYNFSNTGLPDPTLNTTYLQTLRGLCPLNGNLSALVDFD
                     LRTPTIFDNKYYVNLEEQKGLIQSDQELFSSPNATDTIPLVRSFANSTQTFFNAFVEAM
                     DRMGNITPLTGTQGQIRLNCRVVNSNS"
     CDS             2948..3322
                     /codon_start=1
                     /label=SYP
                     /translation="TCKELRDPVTSGLNTSVVFGFLNLVLWVGNLWFVFKETGWAAPFM
                     RAPPGAPEKQPAPGDAYGDAGYGQGPGGYGPQDSYGPQGGYQPDYGQPASGGGGGYGPQ
                     GDYGQQGYGQQGAPTSFSNQM"
     misc_feature    3345..3933
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3816..3827)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3965..4441
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     repeat_region   4481..4621
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4696..5151
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5433..5537
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5538..6398
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6569..7157
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtaagct ttgcaaagat ggataaagtt
      181 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
      241 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
      301 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      361 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      421 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
      481 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
      541 tacttccact ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     1021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     1081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     1141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gtaccggatc ctctagaggc
     1441 caccatggac gtggtgaatc agctggtggc tgggggtcag ttccgggtgg tcaaggagcc
     1501 ccttggcttc gtgaaggtgc tgcagtgggt ctttgccatc ttcgcctttg ctacgtgcgg
     1561 cagctacacc ggagagcttc ggctgagcgt ggagtgtgcc aacaagacgg agagtgccct
     1621 caacatcgaa gtcgaatttg agtacccatt caggctgcac caagtgtact ttgatgcacc
     1681 ctcctgcgtt aaagggggca ctaccaagat cttcctagtt ggtgactact cctcctcggc
     1741 tgaattcttt gtcaccgtgg ctgtgtttgc cttcctctac tccatggggg ccctggccac
     1801 ctacatcttc ctgcagaaca agtaccgaga gaacaacaaa gggccaatga tggacttcct
     1861 ggccacagca gtgttcgctt tcatgtggct agttagctca tccgcctggg ccaaaggcct
     1921 gtccgatgtg aagatggcca ctgacccaga gaacattatc aaggagatgc ctatgtgccg
     1981 ccagacagat atggatctag ccacccagtt aacccctaca ttctacgaca atagctgtcc
     2041 caacgtgtcc aacatcgttc gcgacacaat cgtcaacgag ctcagatccg atcccaggat
     2101 cgctgcttca atattacgtc tgcacttcca tgactgcttc gtgaatggtt gcgacgctag
     2161 catattactg gacaacacca ccagtttccg cactgaaaag gatgcattcg ggaacgctaa
     2221 cagcgccagg ggctttccag tgatcgatcg catgaaggct gccgttgagt cagcatgccc
     2281 acgaacagtc agttgtgcag acctgctgac tatagctgcg caacagagcg tgactcttgc
     2341 aggcggaccg tcctggagag tgccgctcgg tcgacgtgac tccctacagg cattcctaga
     2401 tctggccaac gccaacttgc ctgctccatt cttcaccctg ccccagctga aggatagctt
     2461 tagaaacgtg ggtctgaatc gctcgagtga ccttgtggct ctgtccggag gacacacatt
     2521 tggaaagaac cagtgtaggt tcatcatgga taggctctac aatttcagca acactgggtt
     2581 acctgacccc acgctgaaca ctacgtatct ccagacactg agaggcttgt gcccactgaa
     2641 tggcaacctc agtgcactag tggactttga tctgcggacc ccaaccatct tcgataacaa
     2701 gtactatgtg aatctagagg agcagaaagg cctgatacag agtgatcaag aactgtttag
     2761 cagtccaaac gccactgaca ccatcccact ggtgagaagt tttgctaact ctactcaaac
     2821 cttctttaac gccttcgtgg aagccatgga ccgtatgggt aacattaccc ctctgacggg
     2881 tacccaaggc cagattcgtc tgaactgcag agtggtcaac agcaactcta ccggtggagc
     2941 tcgaatcaca tgcaaggaac tgagggaccc tgtgacttca ggactcaaca cctcggtggt
     3001 gtttggcttc ctgaacctgg tgctctgggt tggcaaccta tggttcgtgt tcaaggagac
     3061 aggctgggcc gccccattca tgcgcgcacc tccaggcgcc ccagaaaagc aaccagctcc
     3121 tggcgatgcc tacggcgatg cgggctatgg gcagggcccc ggaggctatg ggccccagga
     3181 ctcctacggg cctcagggtg gttatcaacc cgattacggg cagccagcca gcggcggtgg
     3241 cggtggctac gggcctcagg gcgactatgg gcagcaaggc tacggccaac agggtgcgcc
     3301 cacctccttc tccaatcaga tgtaacgata tcaagcttat cgataatcaa cctctggatt
     3361 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     3421 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3481 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3541 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3601 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3661 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3721 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct
     3781 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     3841 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     3901 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgagcgctg ctcgagagat
     3961 ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac
     4021 tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt
     4081 gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa
     4141 gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc
     4201 ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga
     4261 gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag
     4321 acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc
     4381 accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct
     4441 tctgattttg taggtaacca cgtgcggacc gagcggccgc aggaacccct agtgatggag
     4501 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     4561 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
     4621 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     4681 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4741 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4801 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4861 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     4921 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4981 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct
     5041 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5101 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     5161 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     5221 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     5281 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     5341 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     5401 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5461 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5521 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5581 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5641 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5701 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5761 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5821 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5881 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5941 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6001 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6061 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6121 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6181 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6241 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6301 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6361 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6421 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6481 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6541 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6601 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6661 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     6721 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6781 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6841 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6901 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6961 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7021 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7081 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7141 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7201 ggccttttgc tcacatgt
//
