LOCUS       Exported                8066 bp ds-DNA     circular SYN 24-JUL-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 24, 2019 from SnapGene 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     misc_feature    854..1714
                     /label=AmpR
                     /note="Unknown feature type:ORF pUC bla Gene Amp Resist."
                     /note="color: #ff0000"
     old_sequence    2319..3041
                     /label=flanking sequence
                     /note="color: #ffff00"
     misc_binding    complement(3042..4039)
                     /label=piggyBacR
                     /note="color: #f7a68a; direction: LEFT"
     primer_bind     4048..4071
                     /label=GTTCCCACAATGGTTAATTCGAGC
                     /note="color: black; sequence: GTTCCCACAATGGTTAATTCGAGC"
     primer_bind     4088..4111
                     /label=GTTCCCACAATGGTTAATTCGAGC
                     /note="color: black; sequence: GTTCCCACAATGGTTAATTCGAGC"
     primer_bind     4128..4151
                     /label=GTTCCCACAATGGTTAATTCGAGC
                     /note="color: black; sequence: GTTCCCACAATGGTTAATTCGAGC"
     misc_feature    4168..5417
                     /label=Transgenesis Marker 3xp3-EGFP
                     /note="Transgenesis Marker 3xp3-EGFP"
                     /note="color: #10ff52"
     misc_feature    4168..4428
                     /label=3xP3
                     /note="3xP3"
                     /note="color: #80ff00"
     primer_bind     4168..4191
                     /label=GTTCCCACAATGGTTAATTCGAGC
                     /note="color: black; sequence: GTTCCCACAATGGTTAATTCGAGC"
     misc_feature    4201..4223
                     /label=Untitled
                     /note="color: #ff8000"
     misc_feature    4447..5166
                     /label=eGFP
                     /note="eGFP"
                     /note="color: #00ff5d"
     misc_feature    5173..5417
                     /label=Sv40
                     /note="Sv40"
                     /note="color: #ff0300"
     misc_feature    5173..5194
                     /label=Untitled
                     /note="color: #ff8000"
     primer_bind     complement(5388..5417)
                     /label=AAGCTTTAAGATACATTGATGAGTTTGGAC
                     /note="color: black; sequence: 
                     AAGCTTTAAGATACATTGATGAGTTTGGAC"
     primer_bind     complement(5455..5484)
                     /label=AAGCTTTAAGATACATTGATGAGTTTGGAC
                     /note="color: black; sequence: 
                     AAGCTTTAAGATACATTGATGAGTTTGGAC"
     misc_feature    5498..6480
                     /label=U6_AAEL017774
                     /note="U6_AAEL017774"
                     /note="color: #00fc00"
     primer_bind     complement(6461..6511)
                     /label=Primer 18
                     /note="color: black; sequence: 1008Ba 
                     GCTCTAAGACCCGGGGGTCAAGATCGCACCATTTCACTACTCTTGCCTCT"
     misc_feature    6481..6500
                     /label=sgWhite2
                     /note="sgWhite2"
                     /note="color: #66ffff"
     misc_feature    6501..6586
                     /label=LgRNA Scaffold
                     /note="LgRNA Scaffold"
                     /note="color: #ff6fcf"
     primer_bind     complement(6566..6615)
                     /label=CGAGACCGTGACCTACATCGTCGACGGCGCGCCAAAAAAGCACCGACTCG
                     /note="color: black; sequence: 
                     CGAGACCGTGACCTACATCGTCGACGGCGCGCCAAAAAAGCACCGACTCGGTGCCACTT
                      T"
     primer_bind     complement(6571..6590)
                     /label=AAAAGCACCGACTCGGTGCC
                     /note="color: black; sequence: AAAAGCACCGACTCGGTGCC"
     primer_bind     6572..6585
                     /label=AAAAGCACCGACTCGGTG
                     /note="color: black; sequence: AAAAGCACCGACTCGGTG"
     primer_bind     complement(6573..6590)
                     /label=AAAAGCACCGACTCGGTG
                     /note="color: black; sequence: AAAAGCACCGACTCGGTG"
     misc_binding    6601..6885
                     /label=attB site
                     /note="attB site"
                     /note="color: #f7a68a"
     primer_bind     6601..6624
                     /label=GTCGACGATGTAGGTCACGGTCTC
                     /note="color: black; sequence: GTCGACGATGTAGGTCACGGTCTC"
     misc_feature    complement(6607..6630)
                     /label=attBseqR Primer 191
                     /note="attBseqR Primer 191"
                     /note="color: #50ff50; direction: LEFT"
     primer_bind     complement(6868..6885)
                     /label=GTCGACATGCCCGCCGTG
                     /note="color: black; sequence: GTCGACATGCCCGCCGTG"
     misc_binding    6894..7594
                     /label=piggyBacL
                     /note="color: #f7a68a"
     old_sequence    7595..7722
                     /label=flanking seq
                     /note="color: #ffff00"
     misc_feature    complement(7723..8066)
                     /label=Untitled
                     /note="color: #ff8000; direction: LEFT"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaaata taacaaaatt
     2341 gtgatcccac aaaatgaagt ggggcaaaat caaataatta atagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcc gtgactataa cacaagttga
     2461 tttaataatt ttagccaaca cgtcgggctg cgtgtttttt gccgacgcgt ctgtgtacac
     2521 gttgattaac tggtcgatta aactgttgaa ataatttaat ttttggttct tctttaaatc
     2581 tgtgatgaaa ttttttaaaa taactttaaa ttcttcattg gtaaaaaatg ccacgttttg
     2641 caacttgtga gggtctaata tgaggtcaaa ctcagtagga gttttatcca aaaaagaaaa
     2701 catgattacg tctgtacacg aacgcgtatt aacgcagagt gcaaagtata agagggttaa
     2761 aaaatatatt ttacgcacca tatacgcatc gggttgatat cgttaatatg gatcaatttg
     2821 aacagttgat taacgtgtct ctgctcaagt ctttgatcaa aacgcaaatc gacgaaaatg
     2881 tgtcggacaa tatcaagtcg atgagcgaaa aactaaaaag gctagaatac gacaatctca
     2941 cagacagcgt tgagatatac ggtattcacg acagcaggct gaataataaa aaaattagaa
     3001 actattattt aaccctagaa agataatcat attgtgacgt acgttaaaga taatcatgcg
     3061 taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt aatttgaata
     3121 gatattaagt tttattatat ttacacttac atactaataa taaattcaac aaacaattta
     3181 tttatgttta tttatttatt aaaaaaaaac aaaaactcaa aatttcttct ataaagtaac
     3241 aaaactttta aacattctct cttttacaaa aataaactta ttttgtactt taaaaacagt
     3301 catgttgtat tataaaataa gtaattagct taacttatac ataatagaaa caaattatac
     3361 ttattagtca gtcagaaaca actttggcac atatcaatat tatgctctcg acaaataact
     3421 tttttgcatt ttttgcacga tgcatttgcc tttcgcctta ttttagaggg gcagtaagta
     3481 cagtaagtac gttttttcat tactggctct tcagtactgt catctgatgt accaggcact
     3541 tcatttggca aaatattaga gatattatcg cgcaaatatc tcttcaaagt aggagcttct
     3601 aaacgcttac gcataaacga tgacgtcagg ctcatgtaaa ggtttctcat aaattttttg
     3661 cgactttgaa ccttttctcc cttgctactg acattatggc tgtatataat aaaagaattt
     3721 atgcaggcaa tgtttatcat tccgtacaat aatgccatag gccacctatt cgtcttccta
     3781 ctgcaggtca tcacagaaca catttggtct agcgtgtcca ctccgccttt agtttgatta
     3841 taatacataa ccatttgcgg tttaccggta ctttcgttga tagaagcatc ctcatcacaa
     3901 gatgataata agtataccat cttagctggc ttcggtttat atgagacgag agtaaggggt
     3961 ccgtcaaaac aaaacatcga tgttcccact ggcctggagc gactgttttt cagtacttcc
     4021 ggtatctcgc gtttgtttga tcgcacggtt cccacaatgg ttaattcgag ctcgcccggg
     4081 cttaagagtt cccacaatgg ttaattcgag ctcgcccggg cttaagagtt cccacaatgg
     4141 ttaattcgag ctcgcccggg cttaagagtt cccacaatgg ttaattcgag ctcgcccggg
     4201 gatctaattc aattagagac taattcaatt agagctaatt caattaggat ccaagcttat
     4261 cgatttcgaa ccctcgaccg ccggagtata aatagaggcg cttcgtctac ggagcgacaa
     4321 ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc
     4381 gcagctgaac aagctaaaca atcggggtac cgctagagtc gacggtacga tccaccggtc
     4441 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     4501 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     4561 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     4621 cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac
     4681 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     4741 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     4801 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     4861 gggcacaagc tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag
     4921 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     4981 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     5041 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     5101 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     5161 aagtaaagcg gccgcgactc tagatcaata atcagccata ccacatttgt agaggtttta
     5221 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     5281 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5341 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5401 aatgtatctt aaagcttcta ggccggccgg gcccctgcat tctagttgtg gtttgtccaa
     5461 actcatcaat gtatcttaaa gcttctaggc cggccgggcc ccacaaacga aactattata
     5521 atcgtcttca ggggaacggt agcatcatat ctgtcaccag tcagtcggat tcctttcttt
     5581 cggcataaat tatgctcggg tgacgagacg agttgaaaat cccgtaaatt aaaacatata
     5641 ccacactcgc cgtcctagac gacgacgacg acgacgattc ccaaggaatt gaacccttca
     5701 tttgcatttc actaccgctc tgagagatgc aaagcgcgcc agacagtcga tcgaatctgc
     5761 agaccagaga gtcacatcaa gaatgttgac attccaccag aagactctaa ttattcttcg
     5821 ctcgactggg aaaactgtta acaactgctc gagtgtggtc ggagcggctc acagtgcgtt
     5881 ttcccgtaga caaaaatcaa atttcatggt atttttcggc gataaacagt aagttaaagg
     5941 gaacccgaat ttagtgctat ataatttaat tccactagag tttgtatcct ttgatagata
     6001 cgcgtatttc gacctcaact gcaaggccgt cgtgtactag acttgactaa tccagactgg
     6061 tcttttagtt atgacttctg tccacatctc catacattca acgcactgtg cggctgtgct
     6121 gtgcgactcc gtcgagtcga ccaacatagt tgaaacaaat tgaatattta attgatcgtt
     6181 ataggaatgg tgttagatga gtcatccttt acagtaagca catacagtat tataattgaa
     6241 gatcgtcggc agataggtgt gtagggtaga gtatcagcaa taagttggga cgtttgactt
     6301 tttgtaggta gacaaaaact aaactttttt tcgcttctct atgtgtgccc ctgggtagcg
     6361 ttccgttccg attggggtgc gaacgaatga aatcgcccat cgagttgata cgtccatcca
     6421 tcgctagaac cgcgttcgct gtagaagact atataagagc agaggcaaga gtagtgaaat
     6481 ggtgcgatct tgacccccgg gtcttagagc tatgctggaa acagcatagc aagttaagat
     6541 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttggcgcgcc
     6601 gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg tgccagggcg tgcccttggg
     6661 ctccccgggc gcgtactcca cctcacccat ctggtccatc atgatgaacg ggtcgaggtg
     6721 gcggtagttg atcccggcga acgcgcggcg caccgggaag ccctcgccct cgaaaccgct
     6781 gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg tcggcgggtg cggatacgcg
     6841 gggcagcgtc agcgggttct cgacggtcac ggcgggcatg tcgacggccg gccgatctcg
     6901 gatctgacaa tgttcagtgc agagactcgg ctacgcctcg tggactttga agttgaccaa
     6961 caatgtttat tcttacctct aatagtcctc tgtggcaagg tcaagattct gttagaagcc
     7021 aatgaagaac ctggttgttc aataacattt tgttcgtcta atatttcact accgcttgac
     7081 gttggctgca cttcatgtac ctcatctata aacgcttctt ctgtatcgct ctggacgtca
     7141 tcttcactta cgtgatctga tatttcactg tcagaatcct caccaacaag ctcgtcatcg
     7201 ctttgcagaa gagcagagag gatatgctca tcgtctaaag aactacccat tttattatat
     7261 attagtcacg atatctataa caagaaaata tatatataat aagttatcac gtaagtagaa
     7321 catgaaataa caatataatt atcgtatgag ttaaatctta aaagtcacgt aaaagataat
     7381 catgcgtcat tttgactcac gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt
     7441 gacaagcacg cctcacggga gctccaagcg gcgactgaga tgtcctaaat gcacagcgac
     7501 ggattcgcgc tatttagaaa gagagagcaa tatttcaaga atgcatgcgt caattttacg
     7561 cagactatct ttctagggtt aaaaaagatt tgcgctttac tcgacctaaa ctttaaacac
     7621 gtcatagaat cttcgtttga caaaaaccac attgtggcca agctgtgtga cgcgacgcgc
     7681 gctaaagaat ggcaaaccaa gtcgcgcgag cgtcgactct agaggatccc cgggtaccga
     7741 gctcgaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7801 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7861 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7921 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7981 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8041 agctcactca aaggcggtaa tacggt
//
