LOCUS       Exported                5377 bp ds-DNA     circular SYN 15-AUG-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Mariana
  TITLE     Direct Submission
  JOURNAL   Exported Sep 24, 2018 from SnapGene Viewer 4.2.5
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        409..1760
                     /label=GAPDH_promoter
     CDS             1761..2135
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      2150..3137
                     /label=GAPDH_terminator
     primer_bind     complement(3156..3172)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3180..3196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3204..3234)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3249..3270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3558..4146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4317..5177)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5178..5282)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcctt gatcttgtga
      421 gggctccact ggaattttcc gacacaagaa agtgccagtg gacaaagggg gaagtgggcc
      481 tcgaaatagc ggtatggtta tgtgagggat acggggcgga gttccggcgt tccctcaggc
      541 cttcctctct gcgcctcagg caagtttcta agaaaagtgc ttttggataa taatcttgta
      601 tggaaatgtg aagcgcaagt gcgcagaacc tagaaaatct aaaacaaaag aaaaaagaga
      661 tcgtcacccg agcagcagaa tcaattcact ccaaggtaag taaggcgtgc tgtgcgggag
      721 cgcccccggc cagtcagtgt tggagcttgg gatgagttgc atctgcgcga agggttggcc
      781 catccatctc aagtcctttc ctggcggttt cgcccgcgtc accagccgcc tggtgctcct
      841 gatggatggg gactctggat ggtcgaaaga ggatgtgttg tatctatctg tctgataagg
      901 taaaagggga cggcttctgt actttccttc ttcgctcgct cccgatcgtg tcttctagga
      961 tgcgcgcttt tgatgtgctg aagattccga gcaccgtgtg ctatgccgtc tccgtcttgc
     1021 ctgtgttggc ggctagctag cgctgagggt tgtggacggc cttgggaatt aggaaaagaa
     1081 agatactcgt gacgttgaaa cgtcctgttc tttttctttt ctagtatttt ctattccagt
     1141 ctttactttc tgttcctttc atttcacttc aagtacatcg tcacctctat cgatctagtg
     1201 tgagccgaat tgagcctcct gtcatgtagc aggaggaact acagaggttc cgactggtac
     1261 gaacaaacga atcggcgaac ggagagtagg catgaagttg ttgttgtcca agatcaaaaa
     1321 gatagaaacg aatatctttc ttgttctgct acctacattg aaacggacat gaaagacgac
     1381 aattctagat gaagacactg ccaaaaggga agaagcgctc ggccaccgca gcaagaaagg
     1441 caagagagag aagataaagt aaattttcga gagaacaaaa gaaatgaatg caggaaggaa
     1501 ggaaggaagg aggggaggta gtgaaagaac gcgtgacaag atttgtaatg aagaacatgg
     1561 catgaaagaa cgaacagggg ggactgacga tttgagggac tgatgtgcgc aattgaatct
     1621 ttttcatttg cattgcggct gcggcggcaa acaaaacaaa aataattatt cggcattcac
     1681 tttgcttgcg ttgttggaca acataaccat aacagaaaca aaagcaagga aacctagcgc
     1741 acacataaac accaagcaaa atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     1801 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     1861 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     1921 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     1981 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     2041 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     2101 actgcgtgca cttcgtggcc gaggagcagg actgagggga tcctctagag tcgacatgta
     2161 cccaatacca caccggtagc ttctcgcggc ggctgacaag aaagattgtt tttacacatt
     2221 tcgaggcatt gatgaccctt atcgacctat cgtctcagat cataaaatgc acgagcatga
     2281 agtacgcgtg ttgtgacttg cttgcgtccc accttctaga tggctttctt cttttcaagt
     2341 gattaaaaca ccacagtaga tgaggactct tagtaagcac tgaagaagcg agtaaatagc
     2401 cctcatcccg tttccctctt ttctaacaca ctttgtttgg aattctaaaa tatcttttat
     2461 catctctttt cattcacaaa actagtattt ctgcattaga aatcattatc ctatctggca
     2521 ctttcactct gacaagaact tgcgtacatg gcggatcctg gcaatcattt tacttgtaca
     2581 gacccaagac tttgtgagtc agtaaatagt aaagaaatgc agaataatca ttagataatt
     2641 gcaaaaccct gatcttcaaa atgttatatc acaagtacct accaagacat ttgtatcttc
     2701 tttttgtctt atgcattttt atttccctta gcgcaattaa aaatattaaa taactgacga
     2761 ctcttttctt aaggattcgc ggtttgctag ttgcactatt gaaaagagca cggtattaat
     2821 ttcatagttt tactttgttg gcaatctcag caaacatggg ttttaatttt aaaattaaat
     2881 aatatttgtg ttagactcaa aagataccta tacaaccctt agggctcatc gtcaacattt
     2941 gcaaattaaa acgattcagc tcctttggta tggtcatgtc tgtttctgat gcactgaaaa
     3001 accttggacg aacgacacat tttattttca ttaggacatc taaaaaacgt tgtaaagagt
     3061 atacaataag cataaaaaag gagaagaaaa aaagaaagac atacatggtt ctgctcttct
     3121 agtgcttcaa ggcatgcaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     3181 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     3241 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     3301 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3361 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3421 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3481 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3541 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3601 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3661 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3721 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3781 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3841 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3901 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3961 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4021 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4081 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4141 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4201 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4261 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4321 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4381 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4441 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4501 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4561 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4621 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4681 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4741 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4801 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4861 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4921 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4981 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5041 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5101 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5161 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5221 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5281 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     5341 acctataaaa ataggcgtat cacgaggccc tttcgtc
//
