LOCUS Exported 4729 bp ds-DNA circular SYN 05-JUL-2018 DEFINITION Cloning vector pSGKP-km, complete sequence. ACCESSION Seq1 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4729) AUTHORS Wang Y, Wang S, Chen W, Song L, Shen Z, Yu F, Li M, Ji Q. TITLE Precise and efficient genome editing in Klebsiella pneumonia using CRISPR-Cas9 and CRISPR-assisted cytidine deaminase JOURNAL unpublished REFERENCE 2 (bases 1 to 4729) AUTHORS Wang Y, Wang S, Chen W, Song L, Shen Z, Yu F, Li M, Ji Q. TITLE Direct Submission JOURNAL Submitted (05-JUL-2018) School of Physical Science and Technology, ShanghaiTech University, No. 100 Haike Road, Shanghai, Shanghai 201210, P.R. China REFERENCE 3 (bases 1 to 4729) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 6, 2018 from SnapGene Viewer 4.2.4 http://www.snapgene.com COMMENT Bankit Comment: Vecscreen Comment:Submitter says that this sequence represents a Cloning Vector Bankit Comment: TAX: No, not new species/combinations; SEE ATTACHMENT Bankit Comment: ALT EMAIL:epub20089@163.com Bankit Comment: TOTAL # OF SEQS:1 ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..4729 /organism="Cloning vector pSGKP-km" /mol_type="other DNA" /label=extrachromosomal /note="extrachromosomal" gene complement(511..1932) /gene="sacB" /label=sacB CDS complement(511..1932) /codon_start=1 /gene="sacB" /product="SacB" /label=sacB /note="sucrose sensitivity marker" /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV VKDSILEQGQLTVNK" regulatory 2318..2352 /regulatory_class="promoter" /note="J23119 promoter" misc_RNA 2370..2452 /product="synthetic-guide RNA, sgRNA" misc_feature 2940..3559 /label=replicon of pMB1 /note="replicon of pMB1" gene complement(3714..4529) /gene="kanR" /label=kanR CDS complement(3714..4529) /codon_start=1 /gene="kanR" /product="KanR" /label=kanR /note="kanamycin resistance protein" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatggtac 421 cgggcccccc ctcgaggtcg acggtatcga tacggcatca gagcagattg tactgagagt 481 gcaccataat cggcattttc ttttgcgttt ttatttgtta actgttaatt gtccttgttc 541 aaggatgctg tctttgacaa cagatgtttt cttgcctttg atgttcagca ggaagctagg 601 cgcaaacgtt gattgtttgt ctgcgtagaa tcctctgttt gtcatatagc ttgtaatcac 661 gacattgttt cctttcgctt gaggtacagc gaagtgtgag taagtaaagg ttacatcgtt 721 aggatcaaga tccattttta acacaaggcc agttttgttc agcggcttgt atgggccagt 781 taaagaatta gaaacataac caagcatgta aatatcgtta gacgtaatgc cgtcaatcgt 841 catttttgat ccgcgggagt cagtgaacag ataccatttg ccgttcattt taaagacgtt 901 cgcgcgttca atttcatctg ttactgtgtt agatgcaatc agcggtttca tcactttttt 961 cagtgtgtaa tcatcgttta gctcaatcat accgagagcg ccgtttgcta actcagccgt 1021 gcgtttttta tcgctttgca gaagtttttg actttcttga cggaagaatg atgtgctttt 1081 gccatagtat gctttgttaa ataaagattc ttcgccttgg tagccatctt cagttccagt 1141 gtttgcttca aatactaagt atttgtggcc tttatcttct acgtagtgag gatctctcag 1201 cgtatggttg tcgcctgagc tgtagttgcc ttcatcgatg aactgctgta cattttgata 1261 cgtttttccg tcaccgtcaa agattgattt ataatcctct acaccgttga tgttcaaaga 1321 gctgtctgat gctgatacgt taacttgtgc agttgtcagt gtttgtttgc cgtaatgttt 1381 accggagaaa tcagtgtaga ataaacggat ttttccgtca gatgtaaatg tggctgaacc 1441 tgaccattct tgtgtttggt cttttaggat agaatcattt gcatcgaatt tgtcgctgtc 1501 tttaaagacg cggccagcgt ttttccagct gtcaatagaa gtttcgccga ctttttgata 1561 gaacatgtaa atcgatgtgt catccgcatt tttaggatct ccggctaatg caaagacgat 1621 gtggtagccg tgatagtttg cgacagtgcc gtcagcgttt tgtaatggcc agctgtccca 1681 aacgtccagg ccttttgcag aagagatatt tttaattgtg gacgaatcga actcaggaac 1741 ttgatatttt tcattttttt gctgttcagg gatttgcagc atatcatggc gtgtaatatg 1801 ggaaatgccg tatgtttcct tatatggctt ttggttcgtt tctttcgcaa acgcttgagt 1861 tgcgcctcct gccagcagtg cggtagtaaa ggttaatact gttgcttgtt ttgcaaactt 1921 tttgatgttc atcgttcatg tctccttttt tatgtactgt gttagcggtc tgcttcttcc 1981 agccctcctg tttgaagatg gcaagttagt tacgcacaat aaaaaaagac ctaaaatatg 2041 taaggggtga cgccaaagta tacactttgc cctttacaca ttttaggtct tgcctgcttt 2101 atcagtaaca aacccgcgcg atttactttt cgacctcatt ctattagact ctcgtttgga 2161 ttgcaactgg tctattttcc tcttttgttt gatagaaaat cataaaagga tttgcagact 2221 acgggcctaa agaactaaaa aatctatctg tttcttttca ttctctgtat tttttatagt 2281 ttctgttgca tgggcataaa gttgcaagct tgatatcttg acagctagct cagtcctagg 2341 tataatacta gtcgagacca ttggtctcag ttttagagct agaaatagca agttaaaata 2401 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttgatatcga 2461 attcctgcag cccgggggat ccactagttc tagagcggcc gccaccgcgg tggagctcat 2521 cccaatggcg cgccgagctt ggctcgagca tggtcatagc tgtttcctgt gtgaaattgt 2581 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 2641 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 2701 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg 2761 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 2821 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 2881 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 2941 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 3001 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 3061 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 3121 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 3181 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 3241 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 3301 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 3361 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 3421 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 3481 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 3541 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 3601 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 3661 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttagaaa 3721 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat 3781 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg 3841 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat 3901 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc 3961 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta 4021 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga 4081 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac 4141 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct 4201 aatacctgga atgctgtttt cccagggatc gcagtggtga gtaaccatgc atcatcagga 4261 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg 4321 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct 4381 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg 4441 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag 4501 caagacgttt cccgttgaat atggctcata ctcttccttt ttcaatatta ttgaagcatt 4561 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 4621 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt 4681 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc //