LOCUS Exported 10256 bp ds-DNA circular SYN 06-JUL-2018 DEFINITION Cloning vector pBECKP-km, complete sequence. ACCESSION Seq1 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10256) AUTHORS Wang Y, Wang S, Chen W, Song L, Shen Z, Yu F, Li M, Ji Q. TITLE Precise and efficient genome editing in Klebsiella pneumonia using CRISPR-Cas9 and CRISPR-assisted cytidine deaminase JOURNAL unpublished REFERENCE 2 (bases 1 to 10256) AUTHORS Wang Y, Wang S, Chen W, Song L, Shen Z, Yu F, Li M, Ji Q. TITLE Direct Submission JOURNAL Submitted (06-JUL-2018) School of Physical Science and Technology, ShanghaiTech University, No. 100 Haike Road, Shanghai, Shanghai 201210, P.R. China REFERENCE 3 (bases 1 to 10256) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 6, 2018 from SnapGene Viewer 4.2.4 http://www.snapgene.com COMMENT Bankit Comment: Vecscreen Comment:Submitter says that this sequence represents a Cloning Vector Bankit Comment: TAX: No, not new species/combinations; SEE ATTACHMENT Bankit Comment: TOTAL # OF SEQS:1 ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..10256 /organism="Cloning vector pBECKP-km" /mol_type="other DNA" /label=extrachromosomal /note="extrachromosomal" gene complement(134..1555) /gene="sacB" /label=sacB CDS complement(134..1555) /codon_start=1 /gene="sacB" /product="SacB" /label=sacB /note="sucrose sensitivity marker" /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV VKDSILEQGQLTVNK" regulatory 1935..2099 /regulatory_class="promoter" /note="rpsLSA promoter" gene 2100..6938 /gene="ncas9-BEC" /label=ncas9-BEC CDS 2100..6938 /codon_start=1 /gene="ncas9-BEC" /product="nCas9-BEC" /label=ncas9-BEC /note="the fusion protein of rAPOBEC1 and spCas9 nickase" /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI LWATGLKSGSETPGTSESATPESDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGN TDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDS FFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIY LALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSA RLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDD DLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDL TLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLV KLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDY FKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFE DREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSD GFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVD ELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENT QLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKN RGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLV ETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNY HHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYS NIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTE VQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLK SVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASA GELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEF SKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKR YTSTKEVLDATLIHQSITGLYETRIDLSQLGGD" misc_feature 2859..2861 /gene="ncas9-BEC" /label=mutation D10A /note="mutation D10A" regulatory 6983..7017 /regulatory_class="promoter" /note="J23119 promoter" misc_RNA 7035..7117 /product="synthetic-guide RNA, sgRNA" gene 8018..8209 /gene="rop" /label=rop CDS 8018..8209 /codon_start=1 /gene="rop" /product="Rop" /label=rop /note="serves to decrease plasmid copy number" /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" misc_feature 8624..9243 /label=replicon of ColE1 /note="replicon of ColE1" gene 9349..10164 /gene="kanR" /label=kanR CDS 9349..10164 /codon_start=1 /gene="kanR" /product="KanR" /label=kanR /note="kanamycin resistance protein" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" ORIGIN 1 tgtaaaacga cggccagtga attgacgcgt attgggatgg taccgggccc cccctcgagg 61 tcgacggtat cgatacggca tcagagcaga ttgtactgag agtgcaccat aatcggcatt 121 ttcttttgcg tttttatttg ttaactgtta attgtccttg ttcaaggatg ctgtctttga 181 caacagatgt tttcttgcct ttgatgttca gcaggaagct aggcgcaaac gttgattgtt 241 tgtctgcgta gaatcctctg tttgtcatat agcttgtaat cacgacattg tttcctttcg 301 cttgaggtac agcgaagtgt gagtaagtaa aggttacatc gttaggatca agatccattt 361 ttaacacaag gccagttttg ttcagcggct tgtatgggcc agttaaagaa ttagaaacat 421 aaccaagcat gtaaatatcg ttagacgtaa tgccgtcaat cgtcattttt gatccgcggg 481 agtcagtgaa cagataccat ttgccgttca ttttaaagac gttcgcgcgt tcaatttcat 541 ctgttactgt gttagatgca atcagcggtt tcatcacttt tttcagtgtg taatcatcgt 601 ttagctcaat cataccgaga gcgccgtttg ctaactcagc cgtgcgtttt ttatcgcttt 661 gcagaagttt ttgactttct tgacggaaga atgatgtgct tttgccatag tatgctttgt 721 taaataaaga ttcttcgcct tggtagccat cttcagttcc agtgtttgct tcaaatacta 781 agtatttgtg gcctttatct tctacgtagt gaggatctct cagcgtatgg ttgtcgcctg 841 agctgtagtt gccttcatcg atgaactgct gtacattttg atacgttttt ccgtcaccgt 901 caaagattga tttataatcc tctacaccgt tgatgttcaa agagctgtct gatgctgata 961 cgttaacttg tgcagttgtc agtgtttgtt tgccgtaatg tttaccggag aaatcagtgt 1021 agaataaacg gatttttccg tcagatgtaa atgtggctga acctgaccat tcttgtgttt 1081 ggtcttttag gatagaatca tttgcatcga atttgtcgct gtctttaaag acgcggccag 1141 cgtttttcca gctgtcaata gaagtttcgc cgactttttg atagaacatg taaatcgatg 1201 tgtcatccgc atttttagga tctccggcta atgcaaagac gatgtggtag ccgtgatagt 1261 ttgcgacagt gccgtcagcg ttttgtaatg gccagctgtc ccaaacgtcc aggccttttg 1321 cagaagagat atttttaatt gtggacgaat cgaactcagg aacttgatat ttttcatttt 1381 tttgctgttc agggatttgc agcatatcat ggcgtgtaat atgggaaatg ccgtatgttt 1441 ccttatatgg cttttggttc gtttctttcg caaacgcttg agttgcgcct cctgccagca 1501 gtgcggtagt aaaggttaat actgttgctt gttttgcaaa ctttttgatg ttcatcgttc 1561 atgtctcctt ttttatgtac tgtgttagcg gtctgcttct tccagccctc ctgtttgaag 1621 atggcaagtt agttacgcac aataaaaaaa gacctaaaat atgtaagggg tgacgccaaa 1681 gtatacactt tgccctttac acattttagg tcttgcctgc tttatcagta acaaacccgc 1741 gcgatttact tttcgacctc attctattag actctcgttt ggattgcaac tggtctattt 1801 tcctcttttg tttgatagaa aatcataaaa ggatttgcag actacgggcc taaagaacta 1861 aaaaatctat ctgtttcttt tcattctctg tattttttat agtttctgtt gcatgggcat 1921 aaagttgcaa gcttgcaaac atgctttggg taaacatgta ggtattaacg tcaatgcgac 1981 aatagtagca ttgattaaat gagaattagt aagtgtttta cttactaaat tttatttaac 2041 ctaaaaatga accacctgga tgtgtgggat taaaaagtga agagaggagg acatatcaca 2101 tgagtagcga aaccggtccg gttgcagtgg atccgaccct gcgccgtcgc attgaaccgc 2161 acgagtttga agtgttcttt gatccgcgcg agctgcgcaa agaaacttgc ctgctgtacg 2221 agattaactg gggtggccgc catagcatct ggcgccatac cagccagaac accaataagc 2281 acgtggaagt gaattttatt gaaaaattta ccaccgagcg ctacttctgc cctaataccc 2341 gctgcagcat cacctggttt ctgagctgga gcccgtgcgg cgaatgtagt cgcgccatta 2401 ccgagttcct gagccgctat ccgcatgtga ccctgttcat ctacatcgcc cgtctgtacc 2461 atcacgccga tccgcgcaat cgccaaggtc tgcgtgatct gattagcagc ggtgtgacca 2521 tccagatcat gaccgaacaa gagagcggct attgctggcg caacttcgtg aactattctc 2581 cgagcaacga agcccactgg ccgcgttatc cgcatctgtg ggtgcgcctg tatgtgctgg 2641 agctgtactg catcatcctg ggcctgccgc cttgcctgaa tattctgcgc cgtaaacagc 2701 cgcaactgac attcttcacc atcgcactgc agagctgcca ttatcagcgc ctgccgccgc 2761 acattttatg ggccaccggt ctgaaaagcg gtagtgaaac tccgggcaca agcgaaagcg 2821 caaccccgga aagtgataag aaatactcaa taggcttagc tatcggcaca aatagcgtcg 2881 gatgggcggt gatcactgat gaatataagg ttccgtctaa aaagttcaag gttctgggaa 2941 atacagaccg ccacagtatc aaaaaaaatc ttataggggc tcttttattt gacagtggag 3001 agacagcgga agcgactcgt ctcaaacgga cagctcgtag aaggtataca cgtcggaaga 3061 atcgtatttg ttatctacag gagatttttt caaatgagat ggcgaaagta gatgatagtt 3121 tctttcatcg acttgaagag tcttttttgg tggaagaaga caagaagcat gaacgtcatc 3181 ctatttttgg aaatatagta gatgaagttg cttatcatga gaaatatcca actatctatc 3241 atctgcgaaa aaaattggta gattctactg ataaagcgga tttgcgctta atctatttgg 3301 ccttagcgca tatgattaag tttcgtggtc attttttgat tgagggagat ttaaatcctg 3361 ataatagtga tgtggacaaa ctatttatcc agttggtaca aacctacaat caattatttg 3421 aagaaaaccc tattaacgca agtggagtag atgctaaagc gattctttct gcacgattga 3481 gtaaatcaag acgattagaa aatctcattg ctcagctccc cggtgagaag aaaaatggct 3541 tatttgggaa tctcattgct ttgtcattgg gtttgacccc taattttaaa tcaaattttg 3601 atttggcaga agatgctaaa ttacagcttt caaaagatac ttacgatgat gatttagata 3661 atttattggc gcaaattgga gatcaatatg ctgatttgtt tttggcagct aagaatttat 3721 cagatgctat tttactttca gatatcctaa gagtaaatac tgaaataact aaggctcccc 3781 tatcagcttc aatgattaaa cgctacgatg aacatcatca agacttgact cttttaaaag 3841 ctttagttcg acaacaactt ccagaaaagt ataaagaaat cttttttgat caatcaaaaa 3901 acggatatgc aggttatatt gatgggggag ctagccaaga agaattttat aaatttatca 3961 aaccaatttt agaaaaaatg gatggtactg aggaattatt ggtgaaacta aatcgtgaag 4021 atttgctgcg caagcaacgg acctttgaca acggctctat tccccatcaa attcacttgg 4081 gtgagctgca tgctattttg agaagacaag aagactttta tccattttta aaagacaatc 4141 gtgagaagat tgaaaaaatc ttgacttttc gaattcctta ttatgttggt ccattggcgc 4201 gtggcaatag tcgttttgca tggatgactc ggaagtctga agaaacaatt accccatgga 4261 attttgaaga agttgtcgat aaaggtgctt cagctcaatc atttattgaa cgcatgacaa 4321 actttgataa aaatcttcca aatgaaaaag tactaccaaa acatagtttg ctttatgagt 4381 attttacggt ttataacgaa ttgacaaagg tcaaatatgt tactgaagga atgcgaaaac 4441 cagcatttct ttcaggtgaa cagaagaaag ccattgttga tttactcttc aaaacaaatc 4501 gaaaagtaac cgttaagcaa ttaaaagaag attatttcaa aaaaatagaa tgttttgata 4561 gtgttgaaat ttcaggagtt gaagatagat ttaatgcttc attaggtacc taccatgatt 4621 tgctaaaaat tattaaagat aaagattttt tggataatga agaaaatgaa gatatcttag 4681 aggatattgt tttaacattg accttatttg aagataggga gatgattgag gaaagactta 4741 aaacatatgc tcacctcttt gatgataagg tgatgaaaca gcttaaacgt cgccgttata 4801 ctggttgggg acgtttgtct cgaaaattga ttaatggtat tagggataag caatctggca 4861 aaacaatatt agattttttg aaatcagatg gttttgccaa tcgcaatttt atgcagctga 4921 tccatgatga tagtttgaca tttaaagaag acattcaaaa agcacaagtg tctggacaag 4981 gcgatagttt acatgaacat attgcaaatt tagctggtag ccctgctatt aaaaaaggta 5041 ttttacagac tgtaaaagtt gttgatgaat tggtcaaagt aatggggcgg cataagccag 5101 aaaatatcgt tattgaaatg gcacgtgaaa atcagacaac tcaaaagggc cagaaaaatt 5161 cgcgagagcg tatgaaacga atcgaagaag gtatcaaaga attaggaagt cagattctta 5221 aagagcatcc tgttgaaaat actcaattgc aaaatgaaaa gctctatctc tattatctcc 5281 aaaatggaag agacatgtat gtggaccaag aattagatat taatcgttta agtgattatg 5341 atgtcgatca cattgttcca caaagtttcc ttaaagacga ttcaatagac aataaggtct 5401 taacgcgttc tgataaaaat cgtggtaaat cggataacgt tccaagtgaa gaagtagtca 5461 aaaagatgaa aaactattgg agacaacttc taaacgccaa gttaatcact caacgtaagt 5521 ttgataattt aacgaaagct gaacgtggag gtttgagtga acttgataaa gctggtttta 5581 tcaaacgcca attggttgaa actcgccaaa tcactaagca tgtggcacaa attttggata 5641 gtcgcatgaa tactaaatac gatgaaaatg ataaacttat tcgagaggtt aaagtgatta 5701 ccttaaaatc taaattagtt tctgacttcc gaaaagattt ccaattctat aaagtacgtg 5761 agattaacaa ttaccatcat gcccatgatg cgtatctaaa tgccgtcgtt ggaactgctt 5821 tgattaagaa atatccaaaa cttgaatcgg agtttgtcta tggtgattat aaagtttatg 5881 atgttcgtaa aatgattgct aagtctgagc aagaaatagg caaagcaacc gcaaaatatt 5941 tcttttactc taatatcatg aacttcttca aaacagaaat tacacttgca aatggagaga 6001 ttcgcaaacg ccctctaatc gaaactaatg gggaaactgg agaaattgtc tgggataaag 6061 ggcgagattt tgccacagtg cgcaaagtat tgtccatgcc ccaagtcaat attgtcaaga 6121 aaacagaagt acagacaggc ggattctcca aggagtcaat tttaccaaaa agaaattcgg 6181 acaagcttat tgctcgtaaa aaagactggg atccaaaaaa atatggtggt tttgatagtc 6241 caacggtagc ttattcagtc ctagtggttg ctaaggtgga aaaagggaaa tcgaagaagt 6301 taaaatccgt taaagagtta ctagggatca caattatgga aagaagttcc tttgaaaaaa 6361 atccgattga ctttttagaa gctaaaggat ataaggaagt taaaaaagac ttaatcatta 6421 aactacctaa atatagtctt tttgagttag aaaacggtcg taaacggatg ctggctagtg 6481 ccggagaatt acaaaaagga aatgagctgg ctctgccaag caaatatgtg aattttttat 6541 atttagctag tcattatgaa aagttgaagg gtagtccaga agataacgaa caaaaacaat 6601 tgtttgtgga gcagcataag cattatttag atgagattat tgagcaaatc agtgaatttt 6661 ctaagcgtgt tattttagca gatgccaatt tagataaagt tcttagtgca tataacaaac 6721 atagagacaa accaatacgt gaacaagcag aaaatattat tcatttattt acgttgacga 6781 atcttggagc tcccgctgct tttaaatatt ttgatacaac aattgatcgt aaacgatata 6841 cgtctacaaa agaagtttta gatgccactc ttatccatca atccatcact ggtctttatg 6901 aaacacgcat tgatttgagt cagctaggag gtgactgatg gctggttggc gtactgttgt 6961 ggtaaatacc aagcttgata tcttgacagc tagctcagtc ctaggtataa tactagtcga 7021 gaccattggt ctcagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 7081 caacttgaaa aagtggcacc gagtcggtgc tttttttgat atcgaattcc tgcagcccgg 7141 gggatccact agttctagag cggccgccac cgcggtggag ctcatcccaa tggcgcgccg 7201 agcttggctc gagcatggtc atagctgttt cctgctcact cattaggcac cgggatctcg 7261 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac 7321 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc 7381 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct 7441 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc 7501 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccc cacgggtgcg 7561 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta 7621 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc 7681 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg 7741 gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc 7801 ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct 7861 ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc 7921 atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac 7981 ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc 8041 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga 8101 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct 8161 ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 8221 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 8281 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag 8341 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 8401 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt 8461 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 8521 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 8581 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 8641 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 8701 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 8761 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 8821 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 8881 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 8941 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 9001 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 9061 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 9121 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 9181 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 9241 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 9301 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt 9361 caacgggaaa cgtcttgctc taggccgcga ttaaattcca acatggatgc tgatttatat 9421 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat 9481 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat 9541 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc 9601 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa 9661 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg 9721 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat 9781 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt 9841 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa 9901 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt 9961 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac 10021 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag 10081 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat 10141 ttgatgctcg atgagttttt ctaagaatta attcatgagc ggatacatat ttgaatgtat 10201 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg //