LOCUS Exported 10584 bp ds-DNA circular SYN 30-NOV-2017 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS tetO.Sox9.Puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10584) AUTHORS Stem Cells Aging and Neurodegeneration TITLE Direct Submission JOURNAL Exported Monday, Sep 10, 2018 from SnapGene 3.3.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10584 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 7..1527 /label=Sox9 CDS 1534..1587 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 1594..2193 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 2232..2820 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS complement(2703..2714) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(2823..2839) /label=KS primer /note="common sequencing primer, one of multiple similar variants" LTR 3345..3525 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" polyA_signal 3557..3781 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 3827..4255 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 4269..4598 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4449..4584 /label=SV40 ori /note="SV40 origin of replication" promoter 4646..4693 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 4712..5086 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 5216..5337 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5386..5402) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 5410..5426 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5434..5464) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5479..5500 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(5788..6376) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6547..7407) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(7408..7512) /gene="bla" /label=AmpR promoter enhancer 7778..8157 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 8159..8357 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" LTR 8375..8555 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 8602..8727 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 9220..9453 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 9980..10097 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 10151..10421 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" promoter 10454..10491 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" ORIGIN 1 gaattcatga atctcctgga ccccttcatg aagatgaccg acgagcagga gaagggcctg 61 tctggcgccc ccagccccac catgtcggag gactcggctg gttcgccctg tccctcgggc 121 tccggctcgg acacggagaa cacccggccc caggagaaca ccttccccaa gggcgagccg 181 gatctgaaga aggagagcga ggaagataag ttccccgtgt gcatccgcga ggcggtcagc 241 caggtgctga agggctacga ctggacgctg gtgcccatgc ccgtgcgcgt caacggctcc 301 agcaagaaca agccacacgt caagcgaccc atgaacgcct tcatggtgtg ggcgcaggct 361 gcgcgcagga agctggcaga ccagtacccg catctgcaca acgcggagct cagcaagact 421 ctgggcaagc tctggaggct gctgaacgag agcgagaaga gacccttcgt ggaggaggcg 481 gagcggctgc gcgtgcagca caagaaagac caccccgatt acaagtacca gccccggcgg 541 aggaagtcgg tgaagaacgg acaagcggag gccgaagagg ccacggaaca gactcacatc 601 tctcctaatg ctatcttcaa ggcgctgcaa gccgactccc cacattcctc ctccggcatg 661 agtgaggtgc actccccggg cgagcactct gggcaatctc agggtccgcc gaccccaccc 721 accactccca aaaccgacgt gcaagctggc aaagttgatc tgaagcgaga ggggcgccct 781 ctggcagagg ggggcagaca gccccccatc gacttccgcg acgtggacat cggtgaactg 841 agcagcgacg tcatctccaa cattgagacc ttcgacgtca atgagtttga ccaatacttg 901 ccacccaacg gccacccagg ggttccggcc acccacggcc aggtcaccta cactggcagt 961 tacggcatca gcagcaccgc acccacccct gcgaccgcgg gccacgtgtg gatgtcgaag 1021 cagcaggcgc cgccccctcc tccgcagcag cctccgcagg ccccgcaagc cccacaggcg 1081 cctccgcagc agcaagcacc cccgcagcag ccgcaggcac cccagcagca gcaggcacac 1141 acgctcacca cgctgagcag cgagccaggc cagtcccagc gaacgcacat caagacggag 1201 cagctgagcc ccagccacta cagcgagcag cagcagcact ccccgcaaca gatctcctac 1261 agccccttca accttcctca ctacagcccc tcctacccgc ccatcacccg ctcgcaatac 1321 gactacgctg accatcagaa ctccggctcc tactacagtc acgcagccgg ccagggctca 1381 gggctctact ccaccttcac ttacatgaac cccgcgcagc gccccatgta cacccccatc 1441 gctgacacct ccggggtccc ttccatcccg cagacccaca gcccgcagca ctgggaacaa 1501 ccagtctaca cacagctcac cagaccctct agagagggca ggggaagtct tctaacatgc 1561 ggggacgtgg aggaaaatcc cggcccactc gagatgaccg agtacaagcc cacggtgcgc 1621 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac 1681 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg 1741 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 1801 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 1861 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 1921 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 1981 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 2041 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 2101 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 2161 acctggtgca tgacccgcaa gcccggtgcc tgattaatta agctagctaa ttcgatatca 2221 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta 2281 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta 2341 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt 2401 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 2461 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 2521 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag 2581 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc 2641 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc 2701 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc 2761 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc 2821 atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag 2881 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc 2941 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 3001 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata 3061 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac 3121 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg 3181 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga 3241 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc 3301 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag 3361 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 3421 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 3481 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc 3541 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 3601 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 3661 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 3721 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 3781 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc 3841 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 3901 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 3961 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 4021 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 4081 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 4141 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 4201 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc 4261 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 4321 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 4381 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 4441 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 4501 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt 4561 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat 4621 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat 4681 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt 4741 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc 4801 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat 4861 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg 4921 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc 4981 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga 5041 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga 5101 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 5161 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 5221 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 5281 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 5341 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 5401 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 5461 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 5521 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 5581 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 5641 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 5701 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 5761 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 5821 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 5881 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 5941 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 6001 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 6061 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 6121 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 6181 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 6241 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 6301 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 6361 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 6421 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 6481 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 6541 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 6601 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 6661 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 6721 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 6781 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 6841 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 6901 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 6961 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 7021 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 7081 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 7141 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 7201 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 7261 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 7321 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 7381 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 7441 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 7501 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct 7561 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag 7621 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct 7681 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt 7741 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat 7801 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca 7861 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 7921 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg 7981 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg 8041 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc 8101 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg 8161 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca 8221 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt 8281 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg 8341 gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg 8401 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 8461 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 8521 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa 8581 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg 8641 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag 8701 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg 8761 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg 8821 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc 8881 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga 8941 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac 9001 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag 9061 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga 9121 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa 9181 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt 9241 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag 9301 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca 9361 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc 9421 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact 9481 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat 9541 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat 9601 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga 9661 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat 9721 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact 9781 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc 9841 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag 9901 agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa 9961 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg 10021 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta 10081 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggttactcg 10141 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc 10201 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga 10261 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc 10321 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa 10381 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcg 10441 gtacccgggt cgagtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg 10501 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg 10561 gaccgatcca gcctccgcgg cccc //