LOCUS       pTn7xTS_mPlum           7339 bp ds-DNA     circular     31-JUL-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     pUC18ori101T-Tn7T-GM_digestConfirmed[TW0222] from 1 to 6189
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    complement(4126..4986)
                     /label=bla
                     /ApEinfo_fwdcolor=#8c8c8c
                     /ApEinfo_revcolor=#8c8c8c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1854..1888)
                     /label=ptacI
                     /ApEinfo_fwdcolor=#8c8c8c
                     /ApEinfo_revcolor=#8c8c8c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(519..684)
                     /label=Tn7L
                     /ApEinfo_fwdcolor=#505150
                     /ApEinfo_revcolor=#505150
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1090..1123)
                     /label=MCS
                     /ApEinfo_fwdcolor=#add300
                     /ApEinfo_revcolor=#add300
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3009..3106
                     /label=Terminator T1
                     /ApEinfo_fwdcolor=#ad1b59
                     /ApEinfo_revcolor=#ad1b59
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1883..1888)
                     /label=-35
                     /ApEinfo_fwdcolor=#add300
                     /ApEinfo_revcolor=#add300
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3203..3297
                     /label=Terminator T0
                     /ApEinfo_fwdcolor=#e600e6
                     /ApEinfo_revcolor=#e600e6
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1861..1866)
                     /label=-10
                     /ApEinfo_fwdcolor=#add300
                     /ApEinfo_revcolor=#add300
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5645..6595
                     /label=repA101
                     /ApEinfo_fwdcolor=#6f2cff
                     /ApEinfo_revcolor=#6f2cff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3346..3544
                     /label=Tn7R
                     /ApEinfo_fwdcolor=#505150
                     /ApEinfo_revcolor=#505150
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6599..7339
                     /label=oriR 101
                     /ApEinfo_fwdcolor=#a03e1e
                     /ApEinfo_revcolor=#a03e1e
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(947..974)
                     /label=trpA terminator
                     /ApEinfo_fwdcolor=#bd020b
                     /ApEinfo_revcolor=#bd020b
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(1894..1913,693..743)
                     /label=MCS(1)
                     /ApEinfo_label=MCS
                     /ApEinfo_fwdcolor=#add300
                     /ApEinfo_revcolor=#add300
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    100..367
                     /label=oriT
                     /ApEinfo_fwdcolor=#9c7627
                     /ApEinfo_revcolor=#9c7627
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1810..1847)
                     /label=t7 leader
                     /ApEinfo_fwdcolor=#d1d1d1
                     /ApEinfo_revcolor=#d1d1d1
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1823..1831)
                     /label=16s homology region
                     /ApEinfo_fwdcolor=#46a4ff
                     /ApEinfo_revcolor=#46a4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1815..1820)
                     /label=SD
                     /ApEinfo_fwdcolor=#46a4ff
                     /ApEinfo_revcolor=#46a4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1126..1806)
                     /label=mPlum
                     /ApEinfo_fwdcolor=#6d3ab1
                     /ApEinfo_revcolor=#6d3ab1
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2980..2986
                     /label=MCS(2)
                     /ApEinfo_label=MCS
                     /ApEinfo_fwdcolor=#add300
                     /ApEinfo_revcolor=#add300
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1938..1985
                     /label=FRT site
                     /ApEinfo_fwdcolor=#ffbf07
                     /ApEinfo_revcolor=#ffbf07
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2906..2953
                     /label=FRT site(1)
                     /ApEinfo_label=FRT site
                     /ApEinfo_fwdcolor=#ffbf07
                     /ApEinfo_revcolor=#ffbf07
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2243..2776
                     /label=Gent R (aacc1)
                     /ApEinfo_fwdcolor=#3a88d4
                     /ApEinfo_revcolor=#3a88d4
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgGTACTATC
       61 AACAGGTTGA ACTGCCCATG TTCTTTCCTG CGTTATCAGA GCTTATCGGC CAGCCTCGCA
      121 GAGCAGGATT CCCGTTGAGC ACCGCCAGGT GCGAATAAGG GACAGTGAAG AAGGAACACC
      181 CGCTCGCGGG TGGGCCTACT TCACCTATCC TGCCCGGCTG ACGCCGTTGG ATACACCAAG
      241 GAAAGTCTAC ACGAACCCTT TGGCAAAATC CTGTATATCG TGCGAAAAAG GATGGATATA
      301 CCGAAAAAAT CGCTATAATG ACCCCGAAGC AGGGTTATGC AGCGGAAAGT ATACCTTAAG
      361 GAATCCCCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGGGTC AGTGAGCGAG
      421 GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA
      481 TGCAGCGCCC AAACATAACA GGAAGAAAAA TGCCCCGCTG TGGGCGGACA AAATAGTTGG
      541 GAACTGGGAG GGGTGGAAAT GGAGTTTTTA AGGATTATTT AGGGAAGAGT GACAAAATAG
      601 ATGGGAACTG GGTGTAGCGT CGTAAGCTAA TACGAAAATT AAAAATGACA AAATAGTTTG
      661 GAACTAGATT TCACTTATCT GGTTGGCCTG CAAGGCCTTC GCGAGGTACC GGGCCCAAGC
      721 TTCTCGAGGA ATTCCTGCAG CCCCCGGTAA ACCGAAAGGC AGGAACAGGA GAGCGCACGA
      781 GGGAGCCGCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCACT
      841 GATTTGAGCG TCAGATTTCG TGATGCTTGT CAGGGGGGCG GAGCCTATGG AAAAACGGCT
      901 TTGCCGCGGC CCTCTCACTT CCCTGTTAAG TATCTCCTAG GCCGAGAAAA AAAAGCCCGC
      961 TCATTAGGCG GGCTAGCTCA TTATTTGTAG AGCTCATCCA TGCCATGTGT AATCCCAGCA
     1021 GCAGTTACAA ACTCAAGAAG GACCATGTGG TCACGCTTTT CGTTGGGATC TTTCGAAAGG
     1081 GCAGATTGTG TCGACCAGCT ATCGATCCAT GGGGCCCGCG GCCGCCTAGG CGCCGGTGGA
     1141 GTGGCGGCCC TCGGCGCGCT CGTACTGTTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT
     1201 GATGTCCAGC TTGATGTCGG TCTTGTAGGC GCCGGGCAGC TGCACGGGCT TCTTGGCCAT
     1261 GTAGGTGGTC TTGACCTCGG CGTCGTAGTG GCCGCCGTCC TTCAGCCTCA GCCTCATCTT
     1321 CATCTCGCCC TTCAGGGCGC CGTCCTCGGG GTACATCCGC TCGGAGGAGG CCTCCCAGCC
     1381 CATGGTCTTC TTCTGCATTA CGGGGCCGTC GGAGGGGAAG TTGGTGCCGC GCACCTTCAC
     1441 CTTGTAGATG AACTCGCCGT CCTGCAGGGA GGAGTCCTGG GTCACGGTCA CCACGCCGCC
     1501 GTCCTCGAAG TTCATCACGC GCTCCCACTT GAAGCCCTCG GGGAAGGACA GCTTCAAGTA
     1561 GTCGGGGATG TCGGCGGGGT GCTTCACGTA GGCCTTGGAG CCGTACATGA TCTGAGGGGA
     1621 CAGGATGTCC CAGGCGAAGG GCAGGGGGCC ACCCTTGGTC ACCTTCAGCC TGGCGGTCTG
     1681 GGTGCCCTCG TAGGGGCGGC CCTCGCCCTC GCCCTCGATC TCGAACTCGT GGCCGTTCAC
     1741 GGAGCCCTCC ATGTGCTCCT TGAAGCGCAT GAACTCCTTG ATGACCTCCT CGCCCTTGCT
     1801 CACCATATGt atatCTCCTT ctTAAAGTTA Aacaaaatta tttctagGGA TCCTCCACAC
     1861 ATTATACGAG CCGATGATTA ATTGTCAAGA ATTGGGGGAT CCACTAGTGA GCTCCGAATT
     1921 AGCTTCAAAA GCGCTCTGAA GTTCCTATAC TTTCTAGAGA ATAGGAACTT CGGAATAGGA
     1981 ACTTCAAGAT CCCCTGATTC CCTTTGTCAA CAGCAATGGA TCGAATTGAC ATAAGCCTGT
     2041 TCGGTTCGTA AACTGTAATG CAAGTAGCGT ATGCGCTCAC GCAACTGGTC CAGAACCTTG
     2101 ACCGAACGCA GCGGTGGTAA CGGCGCAGTG GCGGTTTTCA TGGCTTGTTA TGACTGTTTT
     2161 TTTGTACAGT CTATGCCTCG GGCATCCAAG CAGCAAGCGC GTTACGCCGT GGGTCGATGT
     2221 TTGATGTTAT GGAGCAGCAA CGATGTTACG CAGCAGCAAC GATGTTACGC AGCAGGGCAG
     2281 TCGCCCTAAA ACAAAGTTAG GTGGCTCAAG TATGGGCATC ATTCGCACAT GTAGGCTCGG
     2341 CCCTGACCAA GTCAAATCCA TGCGGGCTGC TCTTGATCTT TTCGGTCGTG AGTTCGGAGA
     2401 CGTAGCCACC TACTCCCAAC ATCAGCCGGA CTCCGATTAC CTCGGGAACT TGCTCCGTAG
     2461 TAAGACATTC ATCGCGCTTG CTGCCTTCGA CCAAGAAGCG GTTGTTGGCG CTCTCGCGGC
     2521 TTACGTTCTG CCCAAGTTTG AGCAGCCGCG TAGTGAGATC TATATCTATG ATCTCGCAGT
     2581 CTCCGGCGAG CACCGGAGGC AGGGCATTGC CACCGCGCTC ATCAATCTCC TCAAGCATGA
     2641 GGCCAACGCG CTTGGTGCTT ATGTGATCTA CGTGCAAGCA GATTACGGTG ACGATCCCGC
     2701 AGTGGCTCTC TATACAAAGT TGGGCATACG GGAAGAAGTG ATGCACTTTG ATATCGACCC
     2761 AAGTACCGCC ACCTAACAAT TCGTTCAAGC CGAGATCGGC TTCCCGGCCG CGGAGTTGTT
     2821 CGGTAAATTG TCACAACGCC GCGGCCAATT CGATCTAGAA TTCCATTGAG TAAGTTTTTA
     2881 AGCACATCAG CTTCAAAAGC GCTCTGAAGT TCCTATACTT TCTAGAGAAT AGGAACTTCG
     2941 GAATAGGTAC TTCAAGATCC CCAATTCGAT CGTCATGCAT GATATCGTCC GGGCCGCAAG
     3001 CTCCTAGCGG CGGATTTGTC CTACTCAGGA GAGCGTTCAC CGACAAACAA CAGATAAAAC
     3061 GAAAGGCCCA GTCTTTCGAC TGAGCCTTTC GTTTTATTTG ATGCCTCAAG CTAGAGAGTc
     3121 attaccccag gcgtttaagG GCACCAATAA CTGCCTTAAA AAAATTACGC CCCGCCCTGC
     3181 CACTCATCGC AGTCTAGCTT GGATTCTCAC CAATAAAAAA CGCCCGGCGG CAACCGAGCG
     3241 TTCTGAACAA ATCCAGATGG AGTTCTGAGG TCATTACTGG ATCTATCAAC AGGAGTCCAA
     3301 GCTCAGCTAA TTAAGCTAGC TTATCGATAC CGTCGACCTC GAACCCCACG CCCCTCTTTA
     3361 ATACGACGGG CAATTTGCAC TTCAGAAAAT GAAGAGTTTG CTTTAGCCAT AACAAAAGTC
     3421 CAGTATGCTT TTTCACAGCA TAACTGGACT GATTTCAGTT TACAACTATT CTGTCTAGTT
     3481 TAAGACTTTA TTGTCATAGT TTAGATCTAT TTTGTTCAGT TTAAGACTTT ATTGTCCGCC
     3541 CACACCCGCT TACGCAGGGC ATCCATTTAT TACTCAACCG TAACCGATTT TGCCAGGTTA
     3601 CGCGGCTGGT CCTCTAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA
     3661 CAGTTGCGCA GCCTGAATGG CGAATGGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG
     3721 TGCGGTATTT CACACCGCAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG
     3781 TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC
     3841 CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT
     3901 TCACCGTCAT CACCGAAACG CGCGAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG
     3961 GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG
     4021 CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA
     4081 CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT
     4141 TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA
     4201 GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC
     4261 GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA
     4321 ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG
     4381 CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA
     4441 GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA
     4501 ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG
     4561 CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG
     4621 GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA
     4681 ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA
     4741 ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT
     4801 GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA
     4861 GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG
     4921 GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT
     4981 TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT
     5041 TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA
     5101 CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA
     5161 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG
     5221 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC
     5281 AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG
     5341 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGC CGGTTGTCAG
     5401 CCGTTAAGTG TTCCTGTGTC ACTCAAAATT GCTTTGAGAG GCTCTAAGGG CTTCTCAGTG
     5461 CGTTACATCC CTGGCTTGTT GTCCACAACC GTTAAACCTT AAAAGCTTTA AAAGCCTTAT
     5521 ATATTCTTTT TTTTCTTAca tgggtatgga cagttttccc tttgatatgt aacggtgaac
     5581 agttgttcta cttttgtttg ttagtcttga tgcttcactg atagatacaa gagccataag
     5641 aacctcagat ccttccgtat ttagccagta tgttctctag tgtggttcgt tgtttttgcg
     5701 tgagccatga gaacgaacca ttgagatcat acttactttg catgtcactc aaaaattttg
     5761 cctcaaaact ggtgagctga atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc
     5821 cgttacgtag gtaggaatct gatgtaatgg ttgttggtat tttgtcacca ttcattttta
     5881 tctggttgtt ctcaagttcg gttacgagat ccatttgtct atctagttca acttggaaaa
     5941 tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa tttcatattg ctgtaagtgt
     6001 ttaaatcttt acttattggt ttcaaaaccc attggttaag ccttttaaac tcatggtagt
     6061 tattttcaag cattaacatg aacttaaatt catcaaggct aatctctata tttgccttgt
     6121 gagttttctt ttgtgttagt tcttttaata accactcata aatcctcata gagtatttgt
     6181 tttcaaaaga cttaacatgt tccagattat attttatgaa tttttttaac tggaaaagat
     6241 aaggcaatat ctcttcacta aaaactaatt ctaatttttc gcttgagaac ttggcatagt
     6301 ttgtccactg gaaaatctca aagcctttaa ccaaaggatt cctgatttcc acagttctcg
     6361 tcatcagctc tctggttgct ttagctaata caccataagc attttcccta ctgatgttca
     6421 tcatctgagc gtattggtta taagtgaacg ataccgtccg ttctttcctt gtagggtttt
     6481 caatcgtggg gttgagtagt gccacacagc ataaaattag cttggtttca tgctccgtta
     6541 agtcatagcg actaatcgct agttcatttg ctttgaaaac aactaattca gacatacatc
     6601 tcaattggtc taggtgattt taatcactat accaattgag atgggctagt caatgataat
     6661 tactagtcct tttcctttga gttgtgggta tctgtaaatt ctgctagacc tttgctggaa
     6721 aacttgtaaa ttctgctaga ccctctgtaa attccgctag acctttgtgt gttttttttg
     6781 tttatattca agtggttata atttatagaa taaagaaaga ataaaaaaag ataaaaagaa
     6841 tagatcccag ccctgtgtat aactcactac tttagtcagt tccgcagtat tacaaaagga
     6901 tgtcgcaaac gctgtttgct cctctacaaa acagacctta aaaccctaaa ggcttaagta
     6961 gcaccctcgc aagctcggtt gcggccgcaa tcgggcaaat cgctgaatat tccttttgtc
     7021 tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac
     7081 ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga ttcatgcaag
     7141 gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg
     7201 tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct ctgattttcc
     7261 agtctgacca cttcggatta tcccgtgaca ggtcattcag actggctaat gcacccagta
     7321 aggcagcggt atcatcaac    
//
