LOCUS       pTn7xKS                 6423 bp ds-DNA     circular     01-AUG-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     pTW88[Ptac-TOX array]_digest-sequence_confirmed[TW0400] from 1 to
            6423
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3705..3733
                     /label=lacIq promoter
                     /ApEinfo_fwdcolor=#44eb9b
                     /ApEinfo_revcolor=#44eb9b
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4381..4401
                     /label=Native HokB Terminator
                     /ApEinfo_fwdcolor=#b5b4b1
                     /ApEinfo_revcolor=#b5b4b1
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4802..5662
                     /label=bla, ampR
                     /ApEinfo_fwdcolor=#d1d1d1
                     /ApEinfo_revcolor=#d1d1d1
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2587..3678)
                     /label=lacI
                     /ApEinfo_fwdcolor=#46a4ff
                     /ApEinfo_revcolor=#46a4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    78..345
                     /label=oriT
                     /ApEinfo_fwdcolor=#c7a768
                     /ApEinfo_revcolor=#c7a768
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    497..662
                     /label=Tn7L
                     /ApEinfo_fwdcolor=#8c8c8c
                     /ApEinfo_revcolor=#8c8c8c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3705..3710)
                     /label=-10
                     /ApEinfo_fwdcolor=#ffbf07
                     /ApEinfo_revcolor=#ffbf07
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(3728..3733)
                     /label=-35
                     /ApEinfo_fwdcolor=#ffbf07
                     /ApEinfo_revcolor=#ffbf07
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1831..1928
                     /label=Terminator: Escherichia coli rrnB T1
                     /ApEinfo_fwdcolor=#ad1b59
                     /ApEinfo_revcolor=#ad1b59
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2025..2119
                     /label=bacteriophage lambda T0
                     /ApEinfo_fwdcolor=#ff0076
                     /ApEinfo_revcolor=#ff0076
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1743..1790
                     /label=FLP site
                     /ApEinfo_fwdcolor=#ffbf07
                     /ApEinfo_revcolor=#ffbf07
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1080..1613
                     /label=Gent acetyl transferase
                     /ApEinfo_fwdcolor=#46a4ff
                     /ApEinfo_revcolor=#46a4ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    775..822
                     /label=FLP site(1)
                     /ApEinfo_label=FLP site
                     /ApEinfo_fwdcolor=#ffbf07
                     /ApEinfo_revcolor=#ffbf07
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    671..748
                     /label=MCS
                     /ApEinfo_fwdcolor=#add300
                     /ApEinfo_revcolor=#add300
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2168..2366
                     /label=Tn7R
                     /ApEinfo_fwdcolor=#8c8c8c
                     /ApEinfo_revcolor=#8c8c8c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5731..6423
                     /label=ColE1
                     /ApEinfo_fwdcolor=#505150
                     /ApEinfo_revcolor=#505150
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3890..4039
                     /label=HokB
                     /ApEinfo_fwdcolor=#b5b4b1
                     /ApEinfo_revcolor=#b5b4b1
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3877..3882
                     /label=RBS
                     /ApEinfo_fwdcolor=#cc0067
                     /ApEinfo_revcolor=#cc0067
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4052..4059
                     /label=RBS region
                     /ApEinfo_fwdcolor=#cc0067
                     /ApEinfo_revcolor=#cc0067
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4061..4068
                     /label=RBS region(1)
                     /ApEinfo_label=RBS region
                     /ApEinfo_fwdcolor=#cc0067
                     /ApEinfo_revcolor=#cc0067
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_binding    3840..3862
                     /label=LacO
                     /ApEinfo_fwdcolor=#6495ed
                     /ApEinfo_revcolor=#6495ed
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3802..3868
                     /label=PtacI
                     /ApEinfo_fwdcolor=#505150
                     /ApEinfo_revcolor=#505150
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3839..3862
                     /label=lac operator
                     /ApEinfo_fwdcolor=#8c8c8c
                     /ApEinfo_revcolor=#8c8c8c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3808..3813
                     /label=-35(1)
                     /ApEinfo_label=-35
                     /ApEinfo_fwdcolor=#b8e203
                     /ApEinfo_revcolor=#b8e203
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3830..3835
                     /label=-10(1)
                     /ApEinfo_label=-10
                     /ApEinfo_fwdcolor=#b8e203
                     /ApEinfo_revcolor=#b8e203
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4343..4363
                     /label=TisB
                     /ApEinfo_fwdcolor=#9a51ff
                     /ApEinfo_revcolor=#9a51ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4394..4401
                     /label=hairpin 3prime
                     /ApEinfo_fwdcolor=#505150
                     /ApEinfo_revcolor=#505150
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4389..4393
                     /label=bubble
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4274..4363
                     /label=TisB(1)
                     /ApEinfo_label=TisB
                     /ApEinfo_fwdcolor=#9a51ff
                     /ApEinfo_revcolor=#9a51ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4381..4388
                     /label=hairpin 5prime
                     /ApEinfo_fwdcolor=#505150
                     /ApEinfo_revcolor=#505150
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4077..4250
                     /label=GhoT
                     /ApEinfo_fwdcolor=#b8e203
                     /ApEinfo_revcolor=#b8e203
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3897..4039
                     /label=HokB(1)
                     /ApEinfo_label=HokB
                     /ApEinfo_fwdcolor=#b5b4b1
                     /ApEinfo_revcolor=#b5b4b1
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4263..4268
                     /label=RBS(1)
                     /ApEinfo_label=RBS
                     /ApEinfo_fwdcolor=#cc0067
                     /ApEinfo_revcolor=#cc0067
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
       61 ctttcctgcg ttatcagggg attccttaag gtatactttc cgctgcataa ccctgcttcg
      121 gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt
      181 gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag
      241 gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg
      301 cggtgctcaa cgggaatcct gctctgcgag gctggccgat aagctctgat accgctcgcc
      361 gcagccgaac gaccgagcgc agcgggtcag tgagcgagga agcggaagag cgcccaatac
      421 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcgcccaa acataacagg
      481 aagaaaaatg ccccgctgtg ggcggacaaa atagttggga actggGAGGG GTGGAAATGG
      541 AGTTTttaag gattatttag ggaagagtga caaaatagat gggaactggg tgtagcgtcg
      601 taagctaata cgaaaattaa aaatgacaaa atagtttgga actagatttc acttatctgg
      661 ttggcctgca aggccttcgc gaggtaccgg gcccaagctt ctcgaggaat tcctgcagcc
      721 cgggggatcc actagtgagc tcatgcatga tcgaattagc ttcaaaagcg ctctgaagtt
      781 cctatacttt ctagagaata ggaacttcgg aataggaact tcaAGATCCC CTGATTCCCT
      841 TTGtcaacag caatggatcg aattgacata agcctgttcg gttcgtaaac tgtaatgcaa
      901 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
      961 cgcagtggcg gttttcatgg cttgttatga ctgttttttt gtacagtcta tgcctcgggc
     1021 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     1081 tgttacgcag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaggtg
     1141 gctcaagtat gggcatcatt cgcacatgta ggctcggccc tgaccaagtc aaatccatgc
     1201 gggctgctct tgatcttttc ggtcgtgagt tcggagacgt agccacctac tcccaacatc
     1261 agccggactc cgattacctc gggaacttgc tccgtagtaa gacattcatc gcgcttgctg
     1321 ccttcgacca agaagcggtt gttggcgctc tcgcggctta cgttctgccc aggtttgagc
     1381 agccgcgtag tgagatctat atctatgatc tcgcagtctc cggcgagcac cggaggcagg
     1441 gcattgccac cgcgctcatc aatctcctca agcatgaggc caacgcgctt ggtgcttatg
     1501 tgatctacgt gcaagcagat tacggtgacg atcccgcagt ggctctctat acaaagttgg
     1561 gcatacggga agaagtgatg cactttgata tcgacccaag taccgccacc taacaattcg
     1621 ttcaagccga gatcggcttc ccggccgcgg agttgttcgg taaattgtca caacgccgcg
     1681 gccaattcga tctagaattc cattgagtaa gtttttaagc acatcagctt caaaagcgct
     1741 ctgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc aagatcccca
     1801 attcgatcgt ccgggccgca agctcctagc ggcggatttg tcctactcag gagagcgttc
     1861 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
     1921 tgatgcctca agctagagag tcattacccc aggcgtttaa gggcaccaat aactgcctta
     1981 aaaaaattac gccccgccct gccactcatc gcagtctagc ttggattctc accaataaaa
     2041 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     2101 ggatctatca acaggagtcc aagctcagct aattaagcta gcttatcgat accgtcgacc
     2161 tcgaacccca cgcccctctt taatacgacg ggcaatttgc acttcagaaa atgaagagtt
     2221 tgctttagcc ataacaaaag tccagtatgc tttttcacag cataactgga ctgatttcag
     2281 tttacaacta ttctgtctag tttaagactt tattgtcata gtttagatct attttgttca
     2341 gtttaagact ttattgtccg cccacacccg cttacgcagg gcatccattt attactcaac
     2401 cgtaaccgat tttgccaggt tacgcggctg gtcctctagc tggcgtaata gcgaagaggc
     2461 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc GATCCgagcc
     2521 ggaagcataa agtgtAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG
     2581 TTGCGCtcaC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC
     2641 GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCC AGGGTGGTTT TTCTTTTCAC
     2701 CAGTGAGACG GGCAACAGCT GATTGCCCTT CACCGCCTGG CCCTGAGAGA GTTGCAGCAA
     2761 GCGGTCCACG CTGGTTTGCC CCAGCAGGCG AAAATCCTGT TTGATGGTGG TTAACGGCGG
     2821 GATATAACAT GAGCTGTCTT CGGTATCGTC GTATCCCACT ATCGAGATAT CCGCACCAAC
     2881 GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC GCCATCTGAT CGTTGGCAAC
     2941 CAGCATCGCA GTGGGAACGA TGCCCTCATT CAGCATTTGC ATGGTTTGTT GAAAACCGGA
     3001 CATGGCACTC CAGTCGCCTT CCCGTTCCGC TATCGGCTGA ATTTGATTGC GAGTGAGATA
     3061 TTTATGCCAG CCAGCCAGAC GCAGACGCGC CGAGACAGAA CTTAATGGGC CCGCTAACAG
     3121 CGCGATTTGC TGGTGACCCA ATGCGACCAG ATGCTCCACG CCCAGTCGCG TACCGTCTTC
     3181 ATGGGAGAAA ATAATACTGT TGATGGGTGT CTGGTCAGAG ACATCAAGAA ATAACGCCGG
     3241 AACATTAGTG CAGGCAGCTT CCACAGCAAT GGCATCCTGG TCATCCAGCG GATAGTTAAT
     3301 GATCAGCCCA CTGACGCGTT GCGCGAGAAG ATTGTGCACC GCCGCTTTAC AGGCTTCGAC
     3361 GCCGCTTCGT TCTACCATCG ACACCACCAC GCTGGCACCC AGTTGATCGG CGCGAGATTT
     3421 AATCGCCGCG ACAATTTGCG ACGGCGCGTG CAGGGCCAGA CTGGAGGTGG CAACGCCAAT
     3481 CAGCAACGAC TGTTTGCCCG CCAGTTGTTG TGCCACGCGG TTGGGAATGT AATTCAGCTC
     3541 CGCCATCGCC GCTTCCACTT TTTCTCGCGT TTTCGCAGAA ACGTGGCTGG CCTGGTTCAC
     3601 CACGCGAGAA ACGGTCTGAT AAGAGACACC GGCATACTCT GCGACATCGT ATAGCGTTAC
     3661 TGGTTTCACA TTCACcacCC TGAATTGACT CTCTTCCGGG CGCTATCATG CCATACCACg
     3721 aaaggttttg caccattcga tgCgcctgat gcggtatttt ctccttacgc atctgtgcgg
     3781 tatttcacac cgcaTATCGA GgagctgTTG ACAattaatc atcggctcgT ATAATgtgtg
     3841 gaattgtgag cggataacaa tttcacacGG CCGGCCaagg agaaaggcta tgaagcACAA
     3901 CCCTCTGGTG GTGTGTCTGC TCATTATCTG CATTACGATT CTGACATTCA CACTCCTGAC
     3961 CCGACAAACG CTCTACGAAC TGCGGTTCCG GGACGGTGAT AAGGAGGTTG CTGCGCTCAT
     4021 GGCCTGCACG TCCAGGTAAT CGCGAGTCGA CAGGAAGGAT AAGAAGAACA TAAAAAATGG
     4081 CACTATTCTC TAAAATATTA ATTTTTTATG TGATTGGTGT GAACATATCC TTTGTCATTA
     4141 TCTGGTTTAT CTCACATGAG AAAACACATA TTCGTTTACT TAGTGCATTC CTGGTCGGAA
     4201 TAACCTGGCC AATGAGTCTG CCTGTGGCAT TACTTTTTTC TCTCTTTTAG GTCGACGGCG
     4261 CCAGGAGACG CGTATGAACC TGGTGGATAT CGCCATTCTT ATCCTCAAAC TCATTGTTGC
     4321 AGCACTGCAA CTGCTTGATG CTgttctgaa atacctgaag taatacTTAA TTAAGGGCAA
     4381 GCGCGGGGAT TTTCCCCGCG CATTTCCCGG GtcaGtatgg tgcactctca gtacaatctg
     4441 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4501 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4561 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     4621 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4681 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4741 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4801 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4861 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4921 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4981 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5041 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5101 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5161 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5221 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5281 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5341 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5401 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5461 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5521 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5581 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5641 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5701 tttaaaactt catttttaat ttaaaaggat CTAGGTGAAG ATCCTTTTTG ATAATCtcat
     5761 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5821 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5881 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5941 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     6001 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6061 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6121 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6181 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6241 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6301 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6361 ccacctctga ctcgagcgtc gatttttgtg atgctcgtca gggggGCGGA GCCTATGGAA
     6421 AAA     
//
