LOCUS       Exported                7346 bp ds-DNA     circular SYN 10-SEP-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2018 from SnapGene Viewer 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7346
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_structure  13..67
                     /label=HRE v2
                     /note="HRE v2"
     misc_feature    32..62
                     /label=HREv2 consensus
                     /note="HREv2 consensus"
     misc_structure  68..106
                     /label=HRE v2
                     /note="HRE v2"
     misc_feature    71..101
                     /label=HREv2 consensus
                     /note="HREv2 consensus"
     misc_structure  107..145
                     /label=HRE v2
                     /note="HRE v2"
     misc_feature    110..140
                     /label=HREv2 consensus
                     /note="HREv2 consensus"
     misc_structure  146..182
                     /label=HRE v2
                     /note="HRE v2"
     misc_feature    149..179
                     /label=HREv2 consensus
                     /note="HREv2 consensus"
     misc_feature    385..460
                     /label=CMV53
                     /note="CMV53"
     misc_feature    433..438
                     /label=TATA Box
                     /note="TATA Box"
     misc_feature    467..478
                     /label=Kozak
                     /note="Kozak"
     misc_feature    476..1189
                     /label=sfGFP
                     /note="sfGFP"
     misc_feature    1237..1461
                     /label=bGHpA
                     /note="bGHpA"
     promoter        1561..2118
                     /label=CMV
                     /note="CMV"
     misc_feature    2237..2914
                     /label=dsRed Express
                     /note="dsRed Express"
     misc_feature    2946..3170
                     /label=bGHpA
                     /note="bGHpA"
     rep_origin      3216..3644
                     /direction=RIGHT
                     /label=RIGHT
                     /note="f1"
     promoter        3649..4019
                     /label=SV40 Early Promoter
                     /note="SV40 Early Promoter"
     CDS             4054..4848
                     /label=Neomycin
                     /note="Neomycin"
     misc_feature    complement(5022..5153)
                     /label=SV40 PA
                     /note="SV40 PA"
     rep_origin      5535..6206
                     /direction=RIGHT
                     /label=RIGHT
                     /note="pUC"
     CDS             complement(6350..7210)
                     /label=Ampicillin
                     /note="Ampicillin"
     promoter        complement(7211..7309)
                     /label=Bla Promoter
                     /note="Bla Promoter"
     misc_feature    7245..7266
                     /label=oYC0547
                     /note="oYC0547"
ORIGIN
        1 gacggatcgg gagatctttg tatttaatta agaccttgag tacgtgcgtc tctgcacgta
       61 tggcgattaa gaccttgagt acgtgcgtct ctgcacgtat ggcgattaag accttgagta
      121 cgtgcgtctc tgcacgtatg gcgattaaga ccttgagtac gtgcgtctct gcacgtatgg
      181 cggatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      241 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
      301 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
      361 gcgttttgcg ctgcttcgcg cgtgcaacaa aatgtcgtaa caagggcggt aggcgtgtac
      421 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg gctagcgccg ccaccatgag
      481 taaaggtgaa gaactgttca ccggtgttgt tccgatcctg gttgaactgg atggtgatgt
      541 taacggccac aaattctctg ttcgtggtga aggtgaaggt gatgcaacca acggtaaact
      601 gaccctgaaa ttcatctgca ctaccggtaa actgccggtt ccatggccga ctctggtgac
      661 taccctgacc tatggtgttc agtgtttttc tcgttacccg gatcacatga agcagcatga
      721 tttcttcaaa tctgcaatgc cggaaggtta tgtacaggag cgcaccattt ctttcaaaga
      781 cgatggcacc tacaaaaccc gtgcagaggt taaatttgaa ggtgatactc tggtgaaccg
      841 tattgaactg aaaggcattg atttcaaaga ggacggcaac atcctgggcc acaaactgga
      901 atataacttc aactcccata acgtttacat caccgcagac aaacagaaga acggtatcaa
      961 agctaacttc aaaattcgcc ataacgttga agacggtagc gtacagctgg cggaccacta
     1021 ccagcagaac actccgatcg gtgatggtcc ggttctgctg ccggataacc actacctgtc
     1081 cacccagtct aaactgtcca aagacccgaa cgaaaagcgc gaccacatgg tgctgctgga
     1141 gttcgttact gcagcaggta tcacgcacgg catggatgaa ctctacaaat aagcggccgc
     1201 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     1261 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     1321 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     1381 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     1441 gggatgcggt gggctctatg gccgcggaat tagcctatgt tttttggaac aattgcatga
     1501 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     1561 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     1621 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     1681 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     1741 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     1801 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     1861 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     1921 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     1981 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2041 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2101 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta
     2161 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
     2221 ctggctagcc gccaccatgg cctcctccga ggacgtcatc aaggagttca tgcgcttcaa
     2281 ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     2341 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
     2401 cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt acgtgaagca
     2461 ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca agtgggagcg
     2521 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     2581 cggctccttc atctacaagg tgaagttcat cggcgtgaac ttcccctccg acggccccgt
     2641 aatgcagaag aagactatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg
     2701 cgtgctgaag ggcgagatcc acaaggccct gaagctgaag gacggcggcc actacctggt
     2761 ggagttcaag tccatctaca tggccaagaa gcccgtgcag ctgcccggct actactacgt
     2821 ggactccaag ctggacatca cctcccacaa cgaggactac accatcgtgg agcagtacga
     2881 gcgcgccgag ggccgccacc acctgttcct gtagacggct gtttaaaccc gctgatcagc
     2941 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     3001 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     3061 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     3121 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     3181 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     3241 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3301 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3361 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3421 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3481 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3541 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     3601 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     3661 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3721 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     3781 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     3841 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     3901 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     3961 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     4021 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     4081 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4141 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4201 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     4261 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     4321 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4381 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4441 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4501 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     4561 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     4621 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     4681 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     4741 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     4801 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     4861 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     4921 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     4981 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     5041 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5101 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     5161 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5221 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5281 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5341 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5401 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5461 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5521 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5581 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5641 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5701 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5761 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5821 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5881 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5941 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6001 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6061 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6121 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6181 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6241 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6301 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     6361 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     6421 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     6481 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     6541 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     6601 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     6661 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     6721 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     6781 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     6841 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     6901 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     6961 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7021 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7081 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7141 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7201 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7261 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7321 ttccccgaaa agtgccacct gacgtc
//
