LOCUS       Exported                7150 bp ds-DNA     circular SYN 10-SEP-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2018 from SnapGene Viewer 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_structure  13..67
                     /label=HRE v2
                     /note="HRE v2"
     misc_feature    32..62
                     /label=HREv2 consensus
                     /note="HREv2 consensus"
     misc_structure  68..106
                     /label=HRE v2
                     /note="HRE v2"
     misc_feature    71..101
                     /label=HREv2 consensus
                     /note="HREv2 consensus"
     misc_structure  107..145
                     /label=HRE v2
                     /note="HRE v2"
     misc_feature    110..140
                     /label=HREv2 consensus
                     /note="HREv2 consensus"
     misc_structure  146..182
                     /label=HRE v2
                     /note="HRE v2"
     misc_feature    149..179
                     /label=HREv2 consensus
                     /note="HREv2 consensus"
     misc_feature    212..252
                     /label=MLP
                     /note="MLP"
     misc_feature    271..282
                     /label=Kozak
                     /note="Kozak"
     misc_feature    280..993
                     /label=sfGFP
                     /note="sfGFP"
     misc_feature    1041..1265
                     /label=bGHpA
                     /note="bGHpA"
     promoter        1365..1922
                     /label=CMV
                     /note="CMV"
     misc_feature    2041..2718
                     /label=dsRed Express
                     /note="dsRed Express"
     misc_feature    2750..2974
                     /label=bGHpA
                     /note="bGHpA"
     rep_origin      3020..3448
                     /direction=RIGHT
                     /label=RIGHT
                     /note="f1"
     promoter        3453..3823
                     /label=SV40 Early Promoter
                     /note="SV40 Early Promoter"
     CDS             3858..4652
                     /label=Neomycin
                     /note="Neomycin"
     misc_feature    complement(4826..4957)
                     /label=SV40 PA
                     /note="SV40 PA"
     rep_origin      5339..6010
                     /direction=RIGHT
                     /label=RIGHT
                     /note="pUC"
     CDS             complement(6154..7014)
                     /label=Ampicillin
                     /note="Ampicillin"
     promoter        complement(7015..7113)
                     /label=Bla Promoter
                     /note="Bla Promoter"
     misc_feature    7049..7070
                     /label=oYC0547
                     /note="oYC0547"
ORIGIN
        1 gacggatcgg gagatctttg tatttaatta agaccttgag tacgtgcgtc tctgcacgta
       61 tggcgattaa gaccttgagt acgtgcgtct ctgcacgtat ggcgattaag accttgagta
      121 cgtgcgtctc tgcacgtatg gcgattaaga ccttgagtac gtgcgtctct gcacgtatgg
      181 cggatcttcg cgattacgca ataatggtac cggggggcta taaaaggggg tgggggcgtt
      241 cgtcctcact ctaagcttga attctctagc gccgccacca tgagtaaagg tgaagaactg
      301 ttcaccggtg ttgttccgat cctggttgaa ctggatggtg atgttaacgg ccacaaattc
      361 tctgttcgtg gtgaaggtga aggtgatgca accaacggta aactgaccct gaaattcatc
      421 tgcactaccg gtaaactgcc ggttccatgg ccgactctgg tgactaccct gacctatggt
      481 gttcagtgtt tttctcgtta cccggatcac atgaagcagc atgatttctt caaatctgca
      541 atgccggaag gttatgtaca ggagcgcacc atttctttca aagacgatgg cacctacaaa
      601 acccgtgcag aggttaaatt tgaaggtgat actctggtga accgtattga actgaaaggc
      661 attgatttca aagaggacgg caacatcctg ggccacaaac tggaatataa cttcaactcc
      721 cataacgttt acatcaccgc agacaaacag aagaacggta tcaaagctaa cttcaaaatt
      781 cgccataacg ttgaagacgg tagcgtacag ctggcggacc actaccagca gaacactccg
      841 atcggtgatg gtccggttct gctgccggat aaccactacc tgtccaccca gtctaaactg
      901 tccaaagacc cgaacgaaaa gcgcgaccac atggtgctgc tggagttcgt tactgcagca
      961 ggtatcacgc acggcatgga tgaactctac aaataagcgg ccgcctagag ggcccgttta
     1021 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     1081 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     1141 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     1201 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     1261 tatggccgcg gaattagcct atgttttttg gaacaattgc atgaagaatc tgcttagggt
     1321 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
     1381 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     1441 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     1501 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1561 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1621 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1681 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1741 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     1801 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     1861 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     1921 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
     1981 ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct agccgccacc
     2041 atggcctcct ccgaggacgt catcaaggag ttcatgcgct tcaaggtgcg catggagggc
     2101 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     2161 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     2221 ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc cgacatcccc
     2281 gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     2341 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggctc cttcatctac
     2401 aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtaatgca gaagaagact
     2461 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
     2521 atccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagtccatc
     2581 tacatggcca agaagcccgt gcagctgccc ggctactact acgtggactc caagctggac
     2641 atcacctccc acaacgagga ctacaccatc gtggagcagt acgagcgcgc cgagggccgc
     2701 caccacctgt tcctgtagac ggctgtttaa acccgctgat cagcctcgac tgtgccttct
     2761 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     2821 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     2881 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     2941 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     3001 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     3061 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     3121 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     3181 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     3241 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     3301 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3361 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3421 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     3481 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3541 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3601 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3661 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3721 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3781 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     3841 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     3901 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     3961 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     4021 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     4081 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     4141 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     4201 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     4261 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     4321 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     4381 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     4441 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     4501 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     4561 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     4621 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     4681 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     4741 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     4801 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     4861 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4921 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     4981 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     5041 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     5101 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     5161 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     5221 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5281 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5341 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5401 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5461 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5521 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5581 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5641 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5701 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5761 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5821 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5881 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt
     5941 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6001 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6061 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6121 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6241 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6301 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7021 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7081 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7141 acctgacgtc
//
