LOCUS       Exported                7172 bp ds-DNA     circular SYN 10-SEP-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2018 from SnapGene Viewer 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_structure  13..67
                     /label=HRE v2
                     /note="HRE v2"
     misc_feature    32..62
                     /label=HREv2 consensus
                     /note="HREv2 consensus"
     misc_structure  68..106
                     /label=HRE v2
                     /note="HRE v2"
     misc_feature    71..101
                     /label=HREv2 consensus
                     /note="HREv2 consensus"
     misc_structure  107..145
                     /label=HRE v2
                     /note="HRE v2"
     misc_feature    110..140
                     /label=HREv2 consensus
                     /note="HREv2 consensus"
     misc_structure  146..182
                     /label=HRE v2
                     /note="HRE v2"
     misc_feature    149..179
                     /label=HREv2 consensus
                     /note="HREv2 consensus"
     misc_feature    212..274
                     /label=miniTK promoter
                     /note="miniTK promoter"
     misc_feature    293..304
                     /label=Kozak
                     /note="Kozak"
     misc_feature    305..1015
                     /label=sfGFP
                     /note="sfGFP"
     misc_feature    1063..1287
                     /label=bGHpA
                     /note="bGHpA"
     promoter        1387..1944
                     /label=CMV
                     /note="CMV"
     misc_feature    2063..2740
                     /label=dsRed Express
                     /note="dsRed Express"
     misc_feature    2772..2996
                     /label=bGHpA
                     /note="bGHpA"
     rep_origin      3042..3470
                     /direction=RIGHT
                     /label=RIGHT
                     /note="f1"
     promoter        3475..3845
                     /label=SV40 Early Promoter
                     /note="SV40 Early Promoter"
     CDS             3880..4674
                     /label=Neomycin
                     /note="Neomycin"
     misc_feature    complement(4848..4979)
                     /label=SV40 PA
                     /note="SV40 PA"
     rep_origin      5361..6032
                     /direction=RIGHT
                     /label=RIGHT
                     /note="pUC"
     CDS             complement(6176..7036)
                     /label=Ampicillin
                     /note="Ampicillin"
     promoter        complement(7037..7135)
                     /label=Bla Promoter
                     /note="Bla Promoter"
     misc_feature    7071..7092
                     /label=oYC0547
                     /note="oYC0547"
ORIGIN
        1 gacggatcgg gagatctttg tatttaatta agaccttgag tacgtgcgtc tctgcacgta
       61 tggcgattaa gaccttgagt acgtgcgtct ctgcacgtat ggcgattaag accttgagta
      121 cgtgcgtctc tgcacgtatg gcgattaaga ccttgagtac gtgcgtctct gcacgtatgg
      181 cggatcttcg cgattacgca ataatggtac cttcgcatat taaggtgacg cgtgtggcct
      241 cgaacaccga gcgaccctgc agcgacccgc ttaaaagctt gaattctcta gcgccgccac
      301 catgagtaaa ggtgaagaac tgttcaccgg tgttgttccg atcctggttg aactggatgg
      361 tgatgttaac ggccacaaat tctctgttcg tggtgaaggt gaaggtgatg caaccaacgg
      421 taaactgacc ctgaaattca tctgcactac cggtaaactg ccggttccat ggccgactct
      481 ggtgactacc ctgacctatg gtgttcagtg tttttctcgt tacccggatc acatgaagca
      541 gcatgatttc ttcaaatctg caatgccgga aggttatgta caggagcgca ccatttcttt
      601 caaagacgat ggcacctaca aaacccgtgc agaggttaaa tttgaaggtg atactctggt
      661 gaaccgtatt gaactgaaag gcattgattt caaagaggac ggcaacatcc tgggccacaa
      721 actggaatat aacttcaact cccataacgt ttacatcacc gcagacaaac agaagaacgg
      781 tatcaaagct aacttcaaaa ttcgccataa cgttgaagac ggtagcgtac agctggcgga
      841 ccactaccag cagaacactc cgatcggtga tggtccggtt ctgctgccgg ataaccacta
      901 cctgtccacc cagtctaaac tgtccaaaga cccgaacgaa aagcgcgacc acatggtgct
      961 gctggagttc gttactgcag caggtatcac gcacggcatg gatgaactct acaaataagc
     1021 ggccgcctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     1081 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     1141 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     1201 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     1261 atgctgggga tgcggtgggc tctatggccg cggaattagc ctatgttttt tggaacaatt
     1321 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     1381 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     1441 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     1501 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     1561 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     1621 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     1681 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1741 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     1801 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     1861 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     1921 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
     1981 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
     2041 cccaagctgg ctagccgcca ccatggcctc ctccgaggac gtcatcaagg agttcatgcg
     2101 cttcaaggtg cgcatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     2161 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc
     2221 cctgcccttc gcctgggaca tcctgtcccc ccagttccag tacggctcca aggtgtacgt
     2281 gaagcacccc gccgacatcc ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg
     2341 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     2401 gcaggacggc tccttcatct acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg
     2461 ccccgtaatg cagaagaaga ctatgggctg ggaggcctcc accgagcgcc tgtacccccg
     2521 cgacggcgtg ctgaagggcg agatccacaa ggccctgaag ctgaaggacg gcggccacta
     2581 cctggtggag ttcaagtcca tctacatggc caagaagccc gtgcagctgc ccggctacta
     2641 ctacgtggac tccaagctgg acatcacctc ccacaacgag gactacacca tcgtggagca
     2701 gtacgagcgc gccgagggcc gccaccacct gttcctgtag acggctgttt aaacccgctg
     2761 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     2821 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     2881 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     2941 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     3001 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     3061 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3121 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3181 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3241 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3301 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3361 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     3421 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     3481 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3541 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3601 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3661 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3721 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     3781 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     3841 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     3901 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     3961 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     4021 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     4081 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     4141 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     4201 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     4261 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     4321 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     4381 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     4441 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     4501 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     4561 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     4621 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     4681 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     4741 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     4801 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     4861 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4921 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     4981 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     5041 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     5101 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     5161 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     5221 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     5281 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     5341 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5401 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5461 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5521 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5581 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5641 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5701 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5761 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5821 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     5881 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5941 ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6001 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6061 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6121 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6181 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6241 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6301 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6361 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6421 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6481 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6541 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6601 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6661 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6721 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6781 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6841 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6901 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6961 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7021 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7081 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7141 gcacatttcc ccgaaaagtg ccacctgacg tc
//
