LOCUS Exported 6468 bp ds-DNA circular SYN 11-SEP-2018 DEFINITION . ACCESSION . VERSION . KEYWORDS pMXs GFP-LC3-RFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6468) AUTHORS . TITLE Direct Submission JOURNAL Exported Tuesday, September 11, 2018 from SnapGene 3.3.4 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6468 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 22..615 /note="long terminal repeat from Moloney murine leukemia virus" /note="color: #ffe4c4" misc_feature 678..1035 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" /note="color: #ffe4c4" CDS 1094..1510 /label=gag (truncated) /note="truncated MMLV gag gene lacking the start codon" /note="color: #993366; direction: RIGHT" CDS 1923..2639 /codon_start=1 /product="enhanced GFP" /label=EGFP /note="mammalian codon-optimized" /note="This feature has 3 segments: 1: 1923 .. 1925 / #05fd14 2: 1926 .. 1928 / #05fd14 / 1a 3: 1929 .. 2639 / #05fd14" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3063..3740 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /note="color: #ff0000" /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" LTR 3973..4442 /note="long terminal repeat from Moloney murine leukemia virus" /note="color: #ffe4c4" rep_origin complement(4743..5331) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(5495..6355) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /note="This feature has 2 segments: 1: 5495 .. 6286 / #ccffcc 2: 6287 .. 6355 / #ccffcc / signal sequence Cleavage site after base 6286" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(6356..6460) /gene="bla" /label=AmpR promoter /note="color: #ffffff; direction: LEFT" ORIGIN 1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta 61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc 121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag 181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat 241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg 301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct 361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg 421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc 481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc 541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag 601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg 661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg 721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg 781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc 841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg 901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct 961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc 1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt 1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa 1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac 1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa 1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc 1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt 1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc 1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc 1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa 1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca 1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca 1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg 1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac 1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc 1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat 1921 ctatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 1981 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 2041 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 2101 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 2161 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 2221 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 2281 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 2341 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 2401 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 2461 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 2521 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 2581 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg 2641 gtggcggtgg ctcgggcggt ggtgggtcgg gtggcggcgg atctatcgat gaattgaatt 2701 ccatgccgtc cgagaagacc ttcaaacagc gccggagctt cgaacaaaga gtggaagatg 2761 tccggctcat ccgggagcag caccccacca agatcccagt gattatagag cgatacaagg 2821 gtgagaagca gctgcccgtc ctggacaaga ccaagttcct tgtacctgat cacgtgaata 2881 tgagcgaact catcaagata attagaaggc gcctgcagct caatgctaac caagccttct 2941 tcctcctggt gaatgggcac agcatggtga gtgtgtccac acccatctct gaagtgtacg 3001 agagcgagag agatgaagac ggcttcctgt acatggtcta tgcctcccag gagacgttcg 3061 ggatggcctc ctccgaggac gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg 3121 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg 3181 gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca 3241 tcctgtcccc tcagttccag tacggctcca aggcctacgt gaagcacccc gccgacatcc 3301 ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg 3361 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct 3421 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga 3481 ccatgggctg ggaggcctcc accgagcgga tgtaccccga ggacggcgcc ctgaagggcg 3541 agatcaagat gaggctgaag ctgaaggacg gcggccacta cgacgccgag gtcaagacca 3601 cctacatggc caagaagccc gtgcagctgc ccggcgccta caagaccgac atcaagctgg 3661 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgagcgc gccgagggcc 3721 gccactccac cggcgcctaa agatcccagt gtggtggtac gggaattcct gcaggcctcg 3781 agggccggcg cgccgcggcc gctacgacga taaaataaaa gattttattt agtctccaga 3841 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat 3901 tttgcaaggc atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa 3961 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg 4021 gctcagggcc aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa 4081 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc 4141 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct 4201 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc 4261 cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact 4321 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt 4381 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt 4441 cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta cattaaatgg 4501 ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 4561 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 4621 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 4681 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 4741 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 4801 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 4861 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 4921 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 4981 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 5041 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 5101 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 5161 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt 5221 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 5281 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 5341 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 5401 gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgcggccggc cgcaaatcaa 5461 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 5521 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 5581 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 5641 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 5701 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 5761 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 5821 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 5881 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 5941 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 6001 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 6061 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 6121 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 6181 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 6241 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 6301 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 6361 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 6421 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttc //