LOCUS       pML2424      9527 bp    DNA   circular            12-MAY-2010
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|553521906|
COMMENT     VNTDBDATE|554063247|
COMMENT     VNTNAME|pML2424|
COMMENT     VNTAUTHORNAME|Michael Niederweis|
FEATURES             Location/Qualifiers
     CDS             277..623
                     /vntifkey="4"
                     /label=sacR
     CDS             640..2061
                     /gene="sacB"
                     /product="Fused with XhoI/Esp31 digested pKS1"
                     /vntifkey="4"
                     /label=sacB
     terminator      6027..6075
                     /vntifkey="43"
                     /label=ttb
     CDS             complement(6207..7205)
                     /vntifkey="4"
                     /label=hyg
                     /note="hygromycin resistance"
     misc_feature    complement(8392..8425)
                     /gene="loxP2"
                     /product="lox site for cre"
                     /vntifkey="21"
                     /label=loxP
     misc_feature    complement(5949..5982)
                     /gene="loxP2"
                     /product="lox site for cre"
                     /vntifkey="21"
                     /label=loxP\(2)
     misc_feature    complement(8137..8137)
                     /vntifkey="21"
                     /label=TSP
     promoter        complement(8063..8365)
                     /vntifkey="30"
                     /label=psmyc
     RBS             complement(8040..8049)
                     /vntifkey="32"
                     /label=SDmyccons
     CDS             complement(7320..8033)
                     /vntifkey="4"
                     /label=mycgfp2+
     rep_origin      2172..2839
                     /vntifkey="33"
                     /label=pUC-origin
     rep_origin      2955..5708
                     /vntifkey="33"
                     /label=PAL5000ts
     misc_feature    8694..8694
                     /vntifkey="21"
                     /label=TSP
     CDS             8810..9514
                     /vntifkey="4"
                     /label=tdTomato
     promoter        8519..8784
                     /vntifkey="30"
                     /label=pwmyc
BASE COUNT     2186 a      2704 c      2714 g      1923 t 
ORIGIN
        1 atctgattta ggatacattt ctagccacct gacgtcgggg gggggggggg aaagccacgt 
       61 tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat 
      121 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga 
      181 aacgtcttgc tcgaccatat acctgccgtt cactattatt tagtgaaatg agatattatg 
      241 atattttctg aattgtgatt aaaaaggcaa ctttatgccc atgcaacaga aactataaaa 
      301 aatacagaga atgaaaagaa acagatagat tttttagttc tttaggcccg tagtctgcaa 
      361 atccttttat gattttctat caaacaaaag aggaaaatag accagttgca atccaaacga 
      421 gagtctaata gaatgaggtc gaaaagtaaa tcgcgcgggt ttgttactga taaagcaggc 
      481 aagacctaaa atgtgtaaag ggcaaagtgt atactttggc gtcacccctt acatatttta 
      541 ggtctttttt tattgtgcgt aactaacttg ccatcttcaa acaggagggc tggaagaagc 
      601 agaccgctaa cacagtacat aaaaaaggag acatgaacga tgaacatcaa aaagtttgca 
      661 aaacaagcaa cagtattaac ctttactacc gcactgctgg caggaggcgc aactcaagcg 
      721 tttgcgaaag aaacgaacca aaagccatat aaggaaacat acggcatttc ccatattaca 
      781 cgccatgata tgctgcaaat ccctgaacag caaaaaaatg aaaaatatca agttcctgaa 
      841 ttcgattcgt ccacaattaa aaatatctct tctgcaaaag gcctggacgt ttgggacagc 
      901 tggccattac aaaacgctga cggcactgtc gcaaactatc acggctacca catcgtcttt 
      961 gcattagccg gagatcctaa aaatgcggat gacacatcga tttacatgtt ctatcaaaaa 
     1021 gtcggcgaaa cttctattga cagctggaaa aacgctggcc gcgtctttaa agacagcgac 
     1081 aaattcgatg caaatgattc tatcctaaaa gaccaaacac aagaatggtc aggttcagcc 
     1141 acatttacat ctgacggaaa aatccgttta ttctacactg atttctccgg taaacattac 
     1201 ggcaaacaaa cactgacaac tgcacaagtt aacgtatcag catcagacag ctctttgaac 
     1261 atcaacggtg tagaggatta taaatcaatc tttgacggtg acggaaaaac gtatcaaaat 
     1321 gtacagcagt tcatcgatga aggcaactac agctcaggcg acaaccatac gctgagagat 
     1381 cctcactacg tagaagataa aggccacaaa tacttagtat ttgaagcaaa cactggaact 
     1441 gaagatggct accaaggcga agaatcttta tttaacaaag catactatgg caaaagcaca 
     1501 tcattcttcc gtcaagaaag tcaaaaactt ctgcaaagcg ataaaaaacg cacggctgag 
     1561 ttagcaaacg gcgctctcgg tatgattgag ctaaacgatg attacacact gaaaaaagtg 
     1621 atgaaaccgc tgattgcatc taacacagta acagatgaaa ttgaacgcgc gaacgtcttt 
     1681 aaaatgaacg gcaaatggta cctgttcact gactcccgcg gatcaaaaat gacgattgac 
     1741 ggcattacgt ctaacgatat ttacatgctt ggttatgttt ctaattcttt aactggccca 
     1801 tacaagccgc tgaacaaaac tggccttgtg ttaaaaatgg atcttgatcc taacgatgta 
     1861 acctttactt actcacactt cgctgtacct caagcgaaag gaaacaatgt cgtgattaca 
     1921 agctatatga caaacagagg attctacgca gacaaacaat caacgtttgc gccaagcttc 
     1981 ctgctgaaca tcaaaggcaa gaaaacatct gttgtcaaag acagcatcct tgaacaagga 
     2041 caattaacag ttaacaaata agcggccgct cgctgcgctc ggtcgttcgg ctgcggcgag 
     2101 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 
     2161 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 
     2221 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 
     2281 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 
     2341 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 
     2401 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 
     2461 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 
     2521 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 
     2581 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 
     2641 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 
     2701 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 
     2761 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 
     2821 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgagtatcga gccgagaacg 
     2881 ttatcgaagt tggtcatgtg taatcccctc gtttgaactt tggattaagc gtagatacac 
     2941 ccttggacaa gccagttgga ttcggagaca agcaaattca gccttaaaaa gggcgaggcc 
     3001 tgcggtggtg gaacaccgca gggcctctaa ccgctcgacg cgctgcacca accagcccgc 
     3061 gaacggctgg cagccagcgt aaggcgcggc tcatcgggcg gcgttcgcca cgatgtcctg 
     3121 cacttcgagc caagcctcga acacctgctg gtgtgcacga ctcacccggt tgttgacacc 
     3181 gcgcgcggcc gtgcgggctc ggtggggcgg ctgtgtcgcc cttgccagcg tgagtagcgc 
     3241 gtacctcacc tcgcccaaca ggtcgcacac agccgattcg tacgccataa agccaggtga 
     3301 gcccaccagc tccgtaagtt cgggcgctgt gtggctcgta cccgcgcatt caggcggcag 
     3361 ggggtctaac gggtctaagg cggcgtgtac ggccgccaca gcggctctca gcggcccgga 
     3421 aacgtcctcg aaacgacgca tgtgttcctc ctggttggta caggtggttg ggggtgctcg 
     3481 gctgtcgctg gtgttccacc accagggctc gacgggagag cgggggagtg tgcagttgtg 
     3541 gggtggcccc tcagcgaaat atctgacttg gagctcgtgt cggaccatac accggtgatt 
     3601 aatcgtggtc tactaccaag cgtgagccac gtcgccgacg aatttgagca gctctggctg 
     3661 ccgtactggc cgctggcaag cgacgatctg ctcgagggga tctaccgcca aagccgcgcg 
     3721 tcggccctag gccgccggta catcgaggcg aacccaacag cgctggcaaa cctgctggtc 
     3781 gtggacgtag accatccaga cgcagcgctc cgagcgctca gcgcccgggg gtcccatccg 
     3841 ctgcccaacg cgatcgtggg caatcgcgcc aacggccacg cacacgcagt gtgggcactc 
     3901 aacgcccctg ttccacgcac cgaatacgcg cggcgtaagc cgctcgcata catggcggcg 
     3961 tgcgccgaag gccttcggcg cgccgtcgac ggcgaccgca gttactcagg cctcatgacc 
     4021 aaaaaccccg gccacatcgc ctgggaaacg gaatggctcc actcagatct ctacacactc 
     4081 agccacatcg aggccgagct cggcgcgaac atgccaccgc cgcgctggcg tcagcagacc 
     4141 acgtacaaag cggctccgac gccgctaggg cggaattgcg cactgttcga ttccgtcagg 
     4201 ttgtgggcct atcgtcccgc cctcatgcgg atctacctgc cgacccggaa cgtggacgga 
     4261 ctcggccgcg cgatctatgc cgagtgccac gcgcgaaacg ccgaattccc gtgcaacgac 
     4321 gtgtgtcccg gaccgctacc ggacagcgag gtccgcgcca tcgccaacag catttggcgt 
     4381 tggatcacaa ccaagtcgcg catttgggcg gacgggatcg tggtctacga ggccacactc 
     4441 agtgcgcgcc agtcggccat ctcgcggaag ggcgcagcag cgcgcacggc ggcgagcaca 
     4501 gttgcgcggc gcgcaaagtc cgcgtcagcc atggaggcat tgctatgagc gacggctaca 
     4561 gcgacggcta cagcgacggc tacaaccggc agccgactgt ccgcaaaaag cggcgcgtga 
     4621 ccgccgccga aggcgctcga atcaccggac tatccgaacg ccacgtcgtc cggctcgtgg 
     4681 cgcaggaacg cagcgagtgg ctcgccgagc aggctgcacg ccgcgaacgc atccgcgcct 
     4741 atcacgacga cgagggccac tcttggccgc aaacggccaa acatttcggg ctgcatctgg 
     4801 acaccgttaa gcgactcggc tatcgggcga ggaaagagcg tgcggcagaa caggaagcgg 
     4861 ctcaaaaggc ccacaacgaa gccgacaatc caccgctgtt ctaacgcaat tggggagcgg 
     4921 gtgtcgcggg ggttccgtgg ggggttccgt tgcaacgggt cggacaggta aaagtcctgg 
     4981 tagacgctag ttttctggtt tgggccatgc ctgtctcgtt gcgtgtttcg ttgcgcccgt 
     5041 tttgaatacc agccagacga gacggggttc tacgaatctt ggtcgatacc aagccatttc 
     5101 cgctgaatat cggggagctc accgccagaa tcggtggttg tggtgatgta cgtggcgaac 
     5161 tccgttgtag tgcctgtggt ggcatccgtg gccactctcg ttgcacggtt cgttgtgccg 
     5221 ttacaggccc cgttgacagc tcaccgaacg tagttaaaac atgctggtca aactaggttt 
     5281 accaacgata cgagtcagct catctagggc cagttctagg cgttgttcgt tgcgcggttc 
     5341 gttgcgcatg tttcgtgtgg ttgctagatg gctccgcaac cacacgcttc gaggttgagt 
     5401 gcttccagca cgggcgcgat ccagaagaac ttcgtcgtgc gactgtcctc gttaacgctc 
     5461 gaagcgatgc cgatcaaatc ggctgtgccg tcgcggttct tgcggtacag cggcgcgcct 
     5521 gagtcgccat gtacggtgcg cagctgagcg gtgatcgtgt acttggcagc cacaacgacc 
     5581 ggcccacagg tctcgccgga tttcatcccg tacttgcaga cctcggtgcc gtacggaaca 
     5641 tctttgcggt ccttcagcac cgcacgcacg gggtatcttc cgccgatttt gaacgaagcg 
     5701 atcggtgttc ccggagcaaa cgcaatcacc ccagcgtcgg tgtagaccgg caatagatca 
     5761 tcgtcgccgg taatcgtggt gccttcgtct ctcgacgcaa cgtaggtacc caacgtccgc 
     5821 tgtgtatccg gtttctctgc aaccgaatac agcaccgggc cccccctcga gtttaaacat 
     5881 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccatgc atgtgctagc 
     5941 tttaattaat aacttcgtat agcatacatt atacgaagtt atacgcgttc ctgcagtcta 
     6001 gagaatagga acttcgagct cgaattcgat aaaataaaaa aggggaccct agaggtcccc 
     6061 ttttttattt tatcgaattc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc 
     6121 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatggccg ccgggggccg 
     6181 ggggcggcgc cgggcggccc ggggcgtcag gcgccggggg cggtgtccgg cggcccccag 
     6241 aggaactgcg ccagttcctc cggatcggtg aagccggaga gatccagcgg ggtctcctcg 
     6301 aacacctcga agtcgtgcag gaaggtgaag gcgagcagtt cgcgggcgaa gtcctcggtc 
     6361 cgcttccact gcgccccgtc gagcagcgcg gccaggatct cgcggtcgcc ccggaaggcg 
     6421 ttgagatgca gttgcaccag gctgtagcgg gagtctcccg catagacgtc ggtgaagtcg 
     6481 acgatcccgg tgacctcggt cgcggccagg tccacgaaga tgttggtccc gtgcaggtcg 
     6541 ccgtggacga accggggttc gcggccggcc agcagcgtgt ccacgtccgg cagccagtcc 
     6601 tccaggcggt ccagcagccg gggcgagagg tagccccacc cgcggtggtc ctcgacggtc 
     6661 gccgcgcggc gttcccgcag cagttccggg aagacctcgg aatggggggt gagcacggtg 
     6721 ttcccggtca gcggcaccct gtgcagccgg ccgagcaccc ggccgagttc gcgggccagg 
     6781 gcgagcagcg cgttccggtc ggtcgtgccg tccatcgcgg accgccaggt ggtgccggtc 
     6841 atccggctca tcaccaggta gggccacggc caggctccgg tgccgggccg cagctcgccg 
     6901 cggccgagga ggcggggcac cggcaccggg gcgtccgcca ggaccgcgta cgcctccgac 
     6961 tccgacgcga ggctctccgg accgcaccag tgctcgccga acagcttgat caccgggtcg 
     7021 ggctcgccga ccagtacggg gttggtgctc tcgccgggca cccgcagcac cggcggcacc 
     7081 ggcagcccga gctcctccag ggctcggcgg gccagcggct cccagaattc ctggtcgttc 
     7141 cgcaggctcg cgtaggaatc atccgaatca atacggtcga gaagtaacag ggattcttgt 
     7201 gtcacagcgg acctctattc acagggtacg ggccggctta attccgcacg gccggtcgcg 
     7261 acacggcctg tccgcaccgc ggatcaggcg ttgacgatga cgggctggtg ataaatctac 
     7321 ttgtacagct cgtccatgcc gtgggtgatg ccggcggcgg tgacgaactc cagcaggacc 
     7381 atgtggtcgc gcttctcgtt cgggtccttg gacagggcgg actgggtgga caggtagtgg 
     7441 ttgtccggca gcaggaccgg gccgtcgccg atcggggtgt tctgctggta gtggtcggct 
     7501 agctggacgc caccgtcctc gatgttgtgg cgggtcttga agttggcctt gatgccgttc 
     7561 ttctgcttgt ccgcggtgat gtagacgttg tgggagttgt agttgtactc cagcttgtgg 
     7621 cccaggatgt taccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttgaccagg 
     7681 gtgtcgccct cgaacttgac ctcggcacgc gtcttgtagt taccgtcgtc cttgaaggag 
     7741 atggtgcgct cctggacgta accctccggc atggcggact tgaagaagtc gtggcgcttc 
     7801 atgtggtccg ggtagcggga gaagcactgg acgccgtagg tcagggtggt gaccagggtc 
     7861 ggccacggga ccggcagctt accggtggtg cagatgaact tcagggtcag cttgccgtag 
     7921 gtggcgtcgc cctcaccctc gccggagacg gagaacttgt ggccgttgac gtcaccgtcc 
     7981 agctcgacca ggatcgggac gacgccggtg aacagctcct cgcccttcga cattattaac 
     8041 ctcctttctg ttataagcat gcggatcgtg ctcatttcgg gcggcgaatc tctcggcgtc 
     8101 gaaatgatgt atgccgtgct ggtcgtacac acgggtactc ccagagccta ctcgaccgag 
     8161 cacacgcagg acaaactcgc ccaccccggg tgggttctcc cgctcgtcag agacccttct 
     8221 cacaccgggc ctgtgcgtgc gaacgcacag atcaacgccg ggatggcgta gagctcactg 
     8281 cgcagcaccg tggggatgcg gccgatcatc acgtcgcgga tcgtgccgcc tcccacggcc 
     8341 gtgacggtgc cgacgatcca atatttctag agtttatctt acagatctgg gataacttcg 
     8401 tatagcatac attatacgaa gttatttaaa tctggcgcgc ctcgtatact cgactgagcg 
     8461 tcagaccccg tagaaaatat caaaggatct tcttggttta aactagtgga tcaatattgg 
     8521 attgccgtgg tgcagggtct cccattctcg gaacccggtg agccgtccca tgttcggggt 
     8581 ttccctgccc tcgtactcac cccggaacgc gacggcgtcg gtccagtagc aggtctcgga 
     8641 gcagtacgtc ctccactggc cgtcgacctt ctcgacgatc tagaattggc acatggcccg 
     8701 tcacaaggag ttcgatccgg acgtcgcact ggacaccgcg atgcgggtgt tctggcgcag 
     8761 cggctacgcg cacacgtcga ccgaggatcc cagaaaggag gaatatataa tggtgagcaa 
     8821 gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa 
     8881 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac 
     8941 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc 
     9001 ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa 
     9061 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg 
     9121 tctggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa 
     9181 gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg 
     9241 ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca 
     9301 ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc 
     9361 caagaagccc gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc 
     9421 ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct 
     9481 gttcctgtac ggcatggacg agctgtacaa gtaacccggg taccgat 
//
