LOCUS       pML2714      7613 bp    DNA   circular            14-FEB-2011
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|581861670|
COMMENT     VNTDBDATE|581861795|
COMMENT     VNTNAME|pML2714|
COMMENT     VNTAUTHORNAME|Alexander Speer|
FEATURES             Location/Qualifiers
     CDS             complement(46..750)
                     /vntifkey="4"
                     /label=pamCherry1m
     promoter        complement(783..901)
                     /vntifkey="30"
                     /label=pimyc
     CDS             complement(6142..7173)
                     /gene="cre"
                     /product="cre"
                     /vntifkey="4"
                     /label=cre
     CDS             complement(5078..5893)
                     /gene="aph"
                     /product="aph"
                     /vntifkey="4"
                     /label=aph
     promoter        complement(7200..7229)
                     /gene="groEL''"
                     /product="groEL"
                     /vntifkey="30"
                     /label=groEL
     rep_origin      1092..1759
                     /vntifkey="33"
                     /label=pUC-origin
     rep_origin      1875..4628
                     /vntifkey="33"
                     /label=PAL5000ts
     terminator      4..22
                     /vntifkey="43"
                     /label=T4g32
BASE COUNT     1641 a      2241 c      2071 g      1659 t         1 others
ORIGIN
        1 aaaggggacc tctagggtcc ccaattaatt agctaaagct tatcacttgt acagctcgtc 
       61 catgccaccg gtcgagtggc ggccctcggc gcgttcgtac tgctccacga tggtgtagtc 
      121 ctcgttgtgc gaggtgatgt ccagcttgcg gttcacgttg taggcacccg gcagctgcac 
      181 cggcttcttg gccttgtacg tggtcttcac ctcggcgtcg tagtgaccgc cgtccttcag 
      241 cttcacgcgc ggcttcacct cgcccttcag ggcgccgtcc tccgggtaca tgcgttccga 
      301 cagggcctcc cagcccatcg tcttcttctg catcacgggg ccgtccgacg ggaagttggt 
      361 gccgcgcagc ttcaccttgt agatgaactc gccgtcctgg agcgagctgt cctgggtcac 
      421 ggtgaccacg ccaccgtcct cgaacttcat cacgcgctcc cacttgaagc cctccgggaa 
      481 cgacagcttg aagtagtccg ggatgtcggc cgggtgcttc acgtaggcgt tcgagccgta 
      541 catgaactgc ggcgacagga tgtcccaggt gaacggcagc ggaccgccct tggtcacctt 
      601 cagcttggcg gtctgggtgc cctcgtacgg gcggccctca ccctcgcctt cgatctcgaa 
      661 cacgtggccg ttcaccgagc cctccatgtg caccttgaag cgcatgaact ccttgatgat 
      721 ggccatgttg tcttcctcgc ccttcgacac catgttctcc cttaattaag catgcggatc 
      781 cgtggcanga gggtcgcctg cgtcggcaac tcgcccaggc tacacgagag ccctcgactg 
      841 cgaaaatgcc gaccaggcac caccgcactc caggcttaac ccggtgcgac gatccaatat 
      901 ttctagagtt taaacagtat taaacgcaaa aaggccatcc gtcaggatgg ccttctgctt 
      961 agctaatcaa ctagttctag agcggccgct cgctgcgctc ggtcgttcgg ctgcggcgag 
     1021 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 
     1081 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 
     1141 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 
     1201 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 
     1261 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 
     1321 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 
     1381 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 
     1441 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 
     1501 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 
     1561 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 
     1621 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 
     1681 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 
     1741 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgagtatcga gccgagaacg 
     1801 ttatcgaagt tggtcatgtg taatcccctc gtttgaactt tggattaagc gtagatacac 
     1861 ccttggacaa gccagttgga ttcggagaca agcaaattca gccttaaaaa gggcgaggcc 
     1921 tgcggtggtg gaacaccgca gggcctctaa ccgctcgacg cgctgcacca accagcccgc 
     1981 gaacggctgg cagccagcgt aaggcgcggc tcatcgggcg gcgttcgcca cgatgtcctg 
     2041 cacttcgagc caagcctcga acacctgctg gtgtgcacga ctcacccggt tgttgacacc 
     2101 gcgcgcggcc gtgcgggctc ggtggggcgg ctgtgtcgcc cttgccagcg tgagtagcgc 
     2161 gtacctcacc tcgcccaaca ggtcgcacac agccgattcg tacgccataa agccaggtga 
     2221 gcccaccagc tccgtaagtt cgggcgctgt gtggctcgta cccgcgcatt caggcggcag 
     2281 ggggtctaac gggtctaagg cggcgtgtac ggccgccaca gcggctctca gcggcccgga 
     2341 aacgtcctcg aaacgacgca tgtgttcctc ctggttggta caggtggttg ggggtgctcg 
     2401 gctgtcgctg gtgttccacc accagggctc gacgggagag cgggggagtg tgcagttgtg 
     2461 gggtggcccc tcagcgaaat atctgacttg gagctcgtgt cggaccatac accggtgatt 
     2521 aatcgtggtc tactaccaag cgtgagccac gtcgccgacg aatttgagca gctctggctg 
     2581 ccgtactggc cgctggcaag cgacgatctg ctcgagggga tctaccgcca aagccgcgcg 
     2641 tcggccctag gccgccggta catcgaggcg aacccaacag cgctggcaaa cctgctggtc 
     2701 gtggacgtag accatccaga cgcagcgctc cgagcgctca gcgcccgggg gtcccatccg 
     2761 ctgcccaacg cgatcgtggg caatcgcgcc aacggccacg cacacgcagt gtgggcactc 
     2821 aacgcccctg ttccacgcac cgaatacgcg cggcgtaagc cgctcgcata catggcggcg 
     2881 tgcgccgaag gccttcggcg cgccgtcgac ggcgaccgca gttactcagg cctcatgacc 
     2941 aaaaaccccg gccacatcgc ctgggaaacg gaatggctcc actcagatct ctacacactc 
     3001 agccacatcg aggccgagct cggcgcgaac atgccaccgc cgcgctggcg tcagcagacc 
     3061 acgtacaaag cggctccgac gccgctaggg cggaattgcg cactgttcga ttccgtcagg 
     3121 ttgtgggcct atcgtcccgc cctcatgcgg atctacctgc cgacccggaa cgtggacgga 
     3181 ctcggccgcg cgatctatgc cgagtgccac gcgcgaaacg ccgaattccc gtgcaacgac 
     3241 gtgtgtcccg gaccgctacc ggacagcgag gtccgcgcca tcgccaacag catttggcgt 
     3301 tggatcacaa ccaagtcgcg catttgggcg gacgggatcg tggtctacga ggccacactc 
     3361 agtgcgcgcc agtcggccat ctcgcggaag ggcgcagcag cgcgcacggc ggcgagcaca 
     3421 gttgcgcggc gcgcaaagtc cgcgtcagcc atggaggcat tgctatgagc gacggctaca 
     3481 gcgacggcta cagcgacggc tacaaccggc agccgactgt ccgcaaaaag cggcgcgtga 
     3541 ccgccgccga aggcgctcga atcaccggac tatccgaacg ccacgtcgtc cggctcgtgg 
     3601 cgcaggaacg cagcgagtgg ctcgccgagc aggctgcacg ccgcgaacgc atccgcgcct 
     3661 atcacgacga cgagggccac tcttggccgc aaacggccaa acatttcggg ctgcatctgg 
     3721 acaccgttaa gcgactcggc tatcgggcga ggaaagagcg tgcggcagaa caggaagcgg 
     3781 ctcaaaaggc ccacaacgaa gccgacaatc caccgctgtt ctaacgcaat tggggagcgg 
     3841 gtgtcgcggg ggttccgtgg ggggttccgt tgcaacgggt cggacaggta aaagtcctgg 
     3901 tagacgctag ttttctggtt tgggccatgc ctgtctcgtt gcgtgtttcg ttgcgcccgt 
     3961 tttgaatacc agccagacga gacggggttc tacgaatctt ggtcgatacc aagccatttc 
     4021 cgctgaatat cggggagctc accgccagaa tcggtggttg tggtgatgta cgtggcgaac 
     4081 tccgttgtag tgcctgtggt ggcatccgtg gccactctcg ttgcacggtt cgttgtgccg 
     4141 ttacaggccc cgttgacagc tcaccgaacg tagttaaaac atgctggtca aactaggttt 
     4201 accaacgata cgagtcagct catctagggc cagttctagg cgttgttcgt tgcgcggttc 
     4261 gttgcgcatg tttcgtgtgg ttgctagatg gctccgcaac cacacgcttc gaggttgagt 
     4321 gcttccagca cgggcgcgat ccagaagaac ttcgtcgtgc gactgtcctc gttaacgctc 
     4381 gaagcgatgc cgatcaaatc ggctgtgccg tcgcggttct tgcggtacag cggcgcgcct 
     4441 gagtcgccat gtacggtgcg cagctgagcg gtgatcgtgt acttggcagc cacaacgacc 
     4501 ggcccacagg tctcgccgga tttcatcccg tacttgcaga cctcggtgcc gtacggaaca 
     4561 tctttgcggt ccttcagcac cgcacgcacg gggtatcttc cgccgatttt gaacgaagcg 
     4621 atcggtgttc ccggagcaaa cgcaatcacc ccagcgtcgg tgtagaccgg caatagatca 
     4681 tcgtcgccgg taatcgtggt gccttcgtct ctcgacgcaa cgtaggtacc caacgtccgc 
     4741 tgtgtatccg gtttctctgc aaccgaatac agcaccgggc cccccctcga gttttgaggt 
     4801 ctgcctcgtg aagaaggtgt tgctgactca taccaggcct gaatcgcccc atcatccagc 
     4861 cagaaagtga gggagccacg gttgatgaga gctttgttgt aggtggacca gttggtgatt 
     4921 ttgaactttt gctttgccac ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc 
     4981 ttcaactcag caaaagttcg atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa 
     5041 tgctctgcca gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca 
     5101 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt 
     5161 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc 
     5221 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa 
     5281 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa 
     5341 gattatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat 
     5401 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc 
     5461 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg 
     5521 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg 
     5581 ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct 
     5641 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa 
     5701 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tggggcttcc 
     5761 catacaagcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc 
     5821 catataaatc agcatccatg ttggaattta atcgcggcct cgagcaagac gtttcccgtt 
     5881 gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc 
     5941 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc 
     6001 tttccccccc ccccgacgtc aggtggctag ctgatcaccg cggccatgat ggcataaaac 
     6061 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggca gtcgatcgta 
     6121 cgctagttaa ctacgtcgac gctaatcgcc atcttccagc aggcgcacca ttgcccctgt 
     6181 ttcactatcc aggttacgga tatagttcat gacaatattt acattggtcc agccaccagc 
     6241 ttgcatgatc tccggtattg aaactccagc gcgggccata tctcgcgcgg ctccgacacg 
     6301 ggcactgtgt ccagaccagg ccaggtatct ctgaccagag tcatccttag cgccgtaaat 
     6361 caatcgatga gttgcttcaa aaatcccttc cagggcgcga gttgatagct ggctggtggc 
     6421 agatggcgcg gcaacaccat tttttctgac ccggcaaaac aggtagttat tcggatcatc 
     6481 agctacacca gagacggaaa tccatcgctc gaccagttta gttaccccca ggctaagtgc 
     6541 cttctctaca cctgcggtgc taaccagcgt tttcgttctg ccaatatgga ttaacattct 
     6601 cccaccgtca gtacgtgaga tatctttaac cctgatcctg gcaatttcgg ctatacgtaa 
     6661 cagggtgtta taagcaatcc ccagaaatgc cagattacgt atatcctggc agcgatcgct 
     6721 attttccatg agtgaacgaa cctggtcgaa atcagtgcgt tcgaacgcta gagcctgttt 
     6781 tgcacgttca ccggcatcaa cgttttcttt tcggatccgc cgcataacca gtgaaacagc 
     6841 attgctgtca cttggtcgtg gcagcccgga ccgacgatga agcatgttta gctggcccaa 
     6901 atgttgctgg atagttttta ctgccagacc gcgcgcctga agatatagaa gataatcgcg 
     6961 aacatcttca ggttctgcgg gaaaccattt ccggttattc aacttgcacc atgccgccca 
     7021 cgaccggcaa acggacagaa gcattttcca ggtatgctca gaaaacgcct ggcgatccct 
     7081 gaacatgtcc atcaggttct tgcgaacctc atcactcgtt gcatcgaccg gtaatgcagg 
     7141 caaattttgg tgtacggtca gtaaattgga catctcgaag tgcctctggc gccaaattct 
     7201 gcagctggat ccgcaattgt cttggccatt gcgaagtgat tcctccggat cggggatgaa 
     7261 accgggggtc accgggtgac ggcaaccccg ctacttacgc tggccgggcg cagtgcccgc 
     7321 gacggacggc tcaagttgtc ctcgctgcca ctcgctgcga cgacgggcct ggcctcaccg 
     7381 tcccgaccta cgactcaccg gtcgcgagtg ccaacgttat tcttagcact cgcctatgcc 
     7441 gagtgcaaga agccccgcac cgggtcatgc ccctcgttcg accttgtcct cggccctccg 
     7501 atccgggtga gtatgttcgg cccatgaccg ccaacgacaa caagacccgt aaatggtcgg 
     7561 ccgcagacgt ccccgatcaa agcgggcgcg tcgttgtggt cacctctaga ttt 
//
