LOCUS       pZac2.1_gfaABC1D_BARK_RGS_p2A_mCherry_WPRE_SV40        6358 bp ds-DNA     circular SYN 13-FEB-2018
DEFINITION  pZac2.1 gfaABC1D-Lck-GCaMP3.
ACCESSION   urn.local...1427301926606.10 (modified)
VERSION     .
KEYWORDS    pZac2.1_gfaABC1D_EzrinGFP-WPRE.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Bal and Khakh
  TITLE     Direct Submission
  JOURNAL   Exported Jan 22, 2018 from SnapGene Viewer 4.1.4
            http://www.snapgene.com
COMMENT     ORIGDB|GenBank.
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        143..172
                     /note="/vntifkey=30"
                     /label="lac_promoter"
     misc_feature    186..208
                     /note="/vntifkey=21"
                     /label="M13_pUC_rev_primer"
     promoter        207..225
                     /note="/vntifkey=30"
                     /label="M13_reverse_primer"
     misc_feature    259..426
                     /label="ITR"
     promoter        449..1129
                     /note="/created_by=User"
                     /note="/vntifkey=30"
                     /modified_by="User"
                     /label="gfaABC1D promoter"
     misc_feature    1239..1371
                     /note="/vntifkey=21"
                     /label="chim_int"
     promoter        1416..1434
                     /note="/vntifkey=30"
                     /label="T7_promoter"
     misc_feature    1456..1461
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     misc_feature    1462..1467
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     source          <1468..>2721
                     /organism="pcDNA3.1-Clover-mRuby2"
                     /mol_type="other DNA"
     CDS             1471..1497
                     /created_by="User"
                     /label="HA"
     flag_tag        1498..1521
                     /created_by="User"
                     /label="FLAG tag"
     cDNA            <1522..1944
                     /created_by="User"
                     /Color="#ee7700"
                     /modified_by="User"
                     /label="BARK-RGS"
     misc_feature    1945..2010
                     /created_by="User"
                     /label="p2A"
                     /note="Geneious type: translational element"
     cDNA            2011..2718
                     /created_by="User"
                     /Color="#ff0000"
                     /modified_by="User"
                     /label="mCherry"
     misc_feature    2722..2727
                     /Recognition_pattern="A^CCGGT"
                     /annotation_group="ACCGGT"
                     /label="AgeI"
                     /note="Geneious type: restriction site"
     enhancer        2748..3335
                     /note="/vntifkey=9"
                     /label="WPRE"
     terminator      3380..3499
                     /note="/vntifkey=43"
                     /label="SV40_PA_terminator"
     misc_feature    3394..3413
                     /note="/vntifkey=21"
                     /label="EBV_rev_primer"
     misc_feature    3628..3795
                     /label="ITR"
     misc_feature    3811..3971
                     /note="/vntifkey=21"
                     /label="lacZ_a"
     promoter        complement(3814..3830)
                     /note="/vntifkey=30"
                     /label="M13_forward20_primer"
     misc_feature    3823..3845
                     /note="/vntifkey=21"
                     /label="M13_pUC_fwd_primer"
     rep_origin      3989..4295
                     /direction="RIGHT"
                     /note="/vntifkey=33"
                     /label="f1_origin"
     promoter        4488..4516
                     /note="/vntifkey=30"
                     /label="AmpR_promoter"
     CDS             4558..5418
                     /codon_start=1
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="ORF\frame\1"
     gene            4558..5418
                     /gene="Ampicillin"
                     /note="/vntifkey=60"
                     /label="Ampicillin"
     rep_origin      5573..6192
                     /direction="RIGHT"
                     /note="/vntifkey=33"
                     /label="pBR322_origin"
ORIGIN      
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccagattta
      241 attaaggcct taattaggct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
      301 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      361 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      421 tctacgtagc catgctctag gaagatctaa catatcctgg tgtggagtag gggacgctgc
      481 tctgacagag gctcgggggc ctgagctggc tctgtgagct ggggaggagg cagacagcca
      541 ggccttgtct gcaagcagac ctggcagcat tgggctggcc gccccccagg gcctcctctt
      601 catgcccagt gaatgactca ccttggcaca gacacaatgt tcggggtggg cacagtgcct
      661 gcttcccgcc gcaccccagc ccccctcaaa tgccttccga gaagcccatt gagcaggggg
      721 cttgcattgc accccagcct gacagcctgg catcttggga taaaagcagc acagccccct
      781 aggggctgcc cttgctgtgt ggcgccaccg gcggtggaga acaaggctct attcagcctg
      841 tgcccaggaa aggggatcag gggatgccca ggcatggaca gtgggtggca gggggggaga
      901 ggagggctgt ctgcttccca gaagtccaag gacacaaatg ggtgagggga gagctctccc
      961 catagctggg ctgcggccca accccacccc ctcaggctat gccagggggt gttgccaggg
     1021 gcacccgggc atcgccagtc tagcccactc cttcataaag ccctcgcatc ccaggagcga
     1081 gcagagccag agcaggttgg agaggagacg catcacctcc gctgctcgca agctttattg
     1141 cggtagttta tcacagttaa attgctaacg cagtcagtgc ttctgacaca acagtctcga
     1201 acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac
     1261 aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct
     1321 gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccact
     1381 cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta taggctagcc
     1441 tcgagctcaa gcttcgaatt caccaccatg tacccatacg atgttcctga ctatgcggac
     1501 tacaaagacg atgacgacaa gaagtacctg gaggaccggg gcgaggtgac ttttgagaag
     1561 atcttctccc agaagctggg gtacctgctt ttccgagact tctgcctgaa gcacctggag
     1621 gaggccaagc ccttggtaga gttctacgag gagatcaaga aatacgagaa gctggagaca
     1681 gaggaggagc gcctggtctg cagccgagag atcttcgaca cgtacatcat gaaggagctg
     1741 ctggcctgct cacatccttt ctcgaagagc gccattgagc acgtccaggg ccatctggtg
     1801 aagaagcagg tgcctccgga tctcttccag ccatatattg aagaaatttg ccagaacctc
     1861 cgaggagacg tgttccagaa attcatcgag agcgataaat tcacacgatt ttgccagtgg
     1921 aagaatgtag agctcaacat ccacggtagt ggcgcaacaa acttctctct gctgaaacaa
     1981 gccggagatg tcgaagagaa tcctggaccg atggtgagca agggcgagga ggataacatg
     2041 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     2101 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     2161 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     2221 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     2281 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     2341 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     2401 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     2461 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     2521 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     2581 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     2641 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     2701 atggacgagc tgtacaagta gaccggtatc atcaagctta tcgataatca acctctggat
     2761 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     2821 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     2881 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     2941 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3001 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3061 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3121 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgccta tgttgccacc
     3181 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3241 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3301 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat acctctagag tcgacccggg
     3361 cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
     3421 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     3481 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     3541 cagggggaga tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
     3601 gataaggatc ttcctagagc atggctacgt agataagtag catggcgggt taatcattaa
     3661 ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     3721 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     3781 cgagcgagcg cgcagcctta attaacctaa ttcactggcc gtcgttttac aacgtcgtga
     3841 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     3901 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     3961 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4021 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4081 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4141 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4201 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4261 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4321 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4381 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     4441 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4501 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4561 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4621 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4681 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4741 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     4801 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     4861 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     4921 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4981 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5041 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5101 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5161 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5221 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5281 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5341 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5401 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5461 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5521 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5581 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5641 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5701 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5761 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5821 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5881 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5941 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6001 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6061 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6121 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6181 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6241 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     6301 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaag
//
