LOCUS       pAAV_CAG_FLEX_BARK_WPRE_SV40        6218 bp ds-DNA     circular SYN 13-SEP-2018
DEFINITION  Expresses BARKrgs AAV backbone, FLEX version (Cre dependent).
ACCESSION   . (modified)
VERSION     .
KEYWORDS    AAV-FLEX-CAG-CyRFP1.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Laviv, T, Kim, BB, Chu, J, Lam, AJ, Lin, MZ, Yasuda and R
  TITLE     Simultaneous dual-color fluorescence lifetime imaging with novel
            red-shifted fluorescent proteins.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4046.
  PUBMED    27798609
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 20, 2017 from SnapGene Console 4.0.0a8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          complement(1..6218)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   complement(56..185)
                     /label="AAV2 ITR"
     primer_bind     complement(67..78)
                     /note="L4440 vector, forward primer"
                     /label="L4440"
     primer_bind     128..139
                     /note="L4440 vector, forward primer"
                     /label="L4440"
     misc_feature    333..338
                     /Recognition_pattern="C^AATTG"
                     /annotation_group="CAATTG"
                     /label="MfeI, MunI"
                     /note="Geneious type: restriction site"
     misc_feature    344..349
                     /Recognition_pattern="GTT^AAC"
                     /annotation_group="GTTAAC"
                     /label="HpaI, KspAI"
                     /note="Geneious type: restriction site"
     polyA_signal    complement(347..468)
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(384..403)
                     /note="SV40 polyA, reverse primer"
                     /label="SV40pA-R"
     primer_bind     438..457
                     /note="SV40 polyA terminator, reverse primer"
                     /label="EBV-rev"
     misc_feature    484..491
                     /Recognition_pattern="GC^GGCCGC"
                     /annotation_group="GCGGCCGC"
                     /label="NotI, CciNI"
                     /note="Geneious type: restriction site"
     misc_feature    485..490
                     /Recognition_pattern="C^GGCCG"
                     /annotation_group="CGGCCG"
                     /label="BseX3I, BstZI, EagI, EclXI, Eco52I"
                     /note="Geneious type: restriction site"
     primer_bind     495..507
                     /note="For pBluescript vector"
                     /label="pBluescriptKS"
     misc_feature    complement(511..1099)
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /label="WPRE"
     CDS             617..628
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
                     /label="Factor Xa site"
     oligo           878..898
                     /created_by="User"
                     /label="co 350"
     primer_bind     1026..1046
                     /note="WPRE, reverse primer"
                     /label="WPRE-R"
     misc_feature    1112..1117
                     /Recognition_pattern="GAT^ATC"
                     /annotation_group="GATATC"
                     /label="EcoRV, Eco32I"
                     /note="Geneious type: restriction site"
     misc_feature    1118..1123
                     /Recognition_pattern="G^AATTC"
                     /annotation_group="GAATTC"
                     /label="EcoRI"
                     /note="Geneious type: restriction site"
     protein_bind    1124..1157
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /label="loxP"
     protein_bind    1208..1241
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GGATACTT). lox2272 sites are compatible with
                     eachother, but incompatible with loxP or loxN sites."
                     /label="lox2272"
     oligo           1232..1268
                     /created_by="User"
                     /label="CO 364"
     oligo           1232..1246
                     /created_by="User"
                     /label="infusion1"
     misc_feature    1242..1247
                     /Recognition_pattern="G^CTAGC"
                     /annotation_group="GCTAGC"
                     /label="NheI, AsuNHI"
                     /note="Geneious type: restriction site"
     oligo           1243..1257
                     /created_by="User"
                     /modified_by="User"
                     /label="infusion2"
     misc_feature    1248..2522
                     /Original_Bases="TTCGGTCGCCACCATGGTGAGCAAGGGCGAGGAGCTGATCA
                     AGGAGAACATGAGAAGCAAGCTGTACCTGGAAGGCAGCGTGAACGGCCACCAGTTCAA
                     GTGCACCCACGAAGGGGAGGGCAAGCCCTACGAGGGCAAGCAGACCAACAGGATCAAG
                     GTGGTGGAGGGAGGCCCCCTGCCGTTCGCATTCGACATCCTGGCCACCATGTTTATGT
                     ACGGGAGCAAGGTGTTCATCAAGTACCCCGCCGACCTCCCCGATTATTTTAAGCAGTC
                     CTTCCCTGAGGGCTTCACATGGGAGAGAGTCATGGTGTTCGAAGACGGGGGCGTGCTG
                     ACCGCCACCCAGGACACCAGCCTCCAGGACGGCGAGCTCATCTACAACGTCAAGCTCA
                     GAGGGGTGAACTTCCCAGCCAACGGCCCCGTGATGCAGAAGAAAACACTGGGCTGGGA
                     GCCCAGCACCGAGACCATGTACCCCGCTGACGGCGGCCTGGAAGGCAGATGCGACAAG
                     GCCCTGAAGCTCGTGGGCGGGGGCCACCTGCACGTCAACTTCAAGACCACATACAAGT
                     CCAAGAAACCCGTGAAGATGCCCGGCGTCCACTACGTGGACCGCAGACTGGAAAGAAT
                     CAAGGAGGCCGACAACGAGACCTACGTCGAGCAGTACGAGCACGCTGTGGCCAGATAC
                     TCCAACCTGGGCGGAGGCATGGACGAGCTGTACAAGTGATATTAT"
                     /label="TTCGGTCGCCACCATGGTGAGCAAGGGCGAGGAGCTGATCAAGGAGAACA
                     TGAGAAGCAAGCTGTACCTGGAAGGCAGCGTGAACGGCCACCAGTTCAAGTGCACCCA
                     CGAAGGGGAGGGCAAGCCCTACGAGGGCAAGCAGACCAACAGGATCAAGGTGGTGGAG
                     GGAGGCCCCCTGCCGTTCGCATTCGACATCCTGGCCACCATGTTTATGTACGGGAGCA
                     AGGTGTTCATCAAGTACCCCGCCGACCTCCCCGATTATTTTAAGCAGTCCTTCCCTGA
                     GGGCTTCACATGGGAGAGAGTCATGGTGTTCGAAGACGGGGGCGTGCTGACCGCCACC
                     CAGGACACCAGCCTCCAGGACGGCGAGCTCATCTACAACGTCAAGCTCAGAGGGGTGA
                     ACTTCCCAGCCAACGGCCCCGTGATGCAGAAGAAAACACTGGGCTGGGAGCCCAGCAC
                     CGAGACCATGTACCCCGCTGACGGCGGCCTGGAAGGCAGATGCGACAAGGCCCTGAAG
                     CTCGTGGGCGGGGGCCACCTGCACGTCAACTTCAAGACCACATACAAGTCCAAGAAAC
                     CCGTGAAGATGCCCGGCGTCCACTACGTGGACCGCAGACTGGAAAGAATCAAGGAGGC
                     CGACAACGAGACCTACGTCGAGCAGTACGAGCACGCTGTGGCCAGATACTCCAACCTG
                     GGCGGAGGCATGGACGAGCTGTACAAGTGATATTAT"
                     /note="Geneious type: Editing History Replacement"
     promoter        <1248..1249
                     /label="T7_promoter"
     source          <1248..>2522
                     /organism="pcDNA3.1-Clover-mRuby2"
                     /mol_type="other DNA"
     source          <1262..>1268
                     /organism="pcDNA3.1-Clover-mRuby2"
                     /mol_type="other DNA"
     infusion        <1272..1278
                     /created_by="User"
                     /modified_by="User"
     CDS             <1272..2522
                     /created_by="User"
                     /modified_by="User"
                     /label="cDNA"
     CDS             1272..1298
                     /created_by="User"
                     /label="HA"
     oligo           complement(<1272..1278)
                     /created_by="User"
                     /modified_by="User"
                     /label="CO 357"
     flag_tag        1299..1322
                     /created_by="User"
                     /label="FLAG tag"
     cDNA            <1323..1745
                     /created_by="User"
                     /label="BARK-RGS"
     misc_feature    1373..1385
                     /Recognition_pattern="(7/12)GAAGNNNNNNTAC(12/7)"
                     /annotation_group="GAAGNNNNNNTAC"
                     /label="BarI"
                     /note="Geneious type: restriction site"
     misc_feature    1381..1386
                     /Recognition_pattern="G^GTACC"
                     /annotation_group="GGTACC"
                     /label="Acc65I, Asp718I"
                     /note="Geneious type: restriction site"
     misc_feature    1381..1386
                     /Recognition_pattern="GGTAC^C"
                     /annotation_group="GGTACC"
                     /label="KpnI"
                     /note="Geneious type: restriction site"
     misc_feature    1415..1425
                     /Recognition_pattern="CCTNN^NNNAGG"
                     /annotation_group="CCTNNNNNAGG"
                     /label="EcoNI, BstENI, XagI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(1607..1613)
                     /Recognition_pattern="CACCTGC(4/8)"
                     /annotation_group="CACCTGC"
                     /label="AarI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(1666..1671)
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="Esp3I, BsmBI"
                     /note="Geneious type: restriction site"
     oligo           complement(1722..1791)
                     /created_by="User"
                     /label="JN2"
     misc_feature    1731..1736
                     /Recognition_pattern="GAG^CTC"
                     /annotation_group="GAGCTC"
                     /label="Ecl136II, Eco53kI, EcoICRI"
                     /note="Geneious type: restriction site"
     misc_feature    1731..1736
                     /Recognition_pattern="GAGCT^C"
                     /annotation_group="GAGCTC"
                     /label="SacI, Psp124BI, SstI"
                     /note="Geneious type: restriction site"
     ORF             <1746..>1811
                     /created_by="User"
                     /modified_by="User"
     misc_feature    1746..1811
                     /created_by="User"
                     /label="p2A"
                     /note="Geneious type: translational element"
     oligo           1771..1830
                     /created_by="User"
                     /label="JN3"
     gene            1812..2519
                     /created_by="User"
                     /Color="#ff0000"
                     /modified_by="User"
                     /label="mCherry"
     oligo           complement(1904..1923)
                     /created_by="User"
                     /label="CO 161"
     misc_feature    2162..2169
                     /Recognition_pattern="CCTGCA^GG"
                     /annotation_group="CCTGCAGG"
                     /label="Sse8387I, SbfI, SdaI"
                     /note="Geneious type: restriction site"
     misc_feature    2256..2266
                     /Recognition_pattern="(8/13)GAGNNNNNCTC(13/8)"
                     /annotation_group="GAGNNNNNCTC"
                     /label="BplI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(2349..2355)
                     /Recognition_pattern="CCTNAGC(-5/-2)"
                     /annotation_group="CCTNAGC"
                     /label="Bpu10I"
                     /note="Geneious type: restriction site"
     misc_feature    complement(2349..2355)
                     /Recognition_pattern="CCTCAGC(-5/-2)"
                     /annotation_group="CCTCAGC"
                     /label="BbvCI"
                     /note="Geneious type: restriction site"
     misc_feature    2491..2505
                     /Recognition_pattern="CCANNNNN^NNNNTGG"
                     /annotation_group="CCANNNNNNNNNTGG"
                     /label="XcmI"
                     /note="Geneious type: restriction site"
     misc_feature    2492..2499
                     /Recognition_pattern="CR^CCGGYG"
                     /annotation_group="CRCCGGYG"
                     /label="SgrAI"
                     /note="Geneious type: restriction site"
     oligo           complement(2500..2539)
                     /created_by="User"
                     /label="CO 365"
     misc_feature    2512..2517
                     /Recognition_pattern="T^GTACA"
                     /annotation_group="TGTACA"
                     /label="Bsp1407I, BsrGI, BstAUI"
                     /note="Geneious type: restriction site"
     misc_feature    2523..2530
                     /Recognition_pattern="GG^CGCGCC"
                     /annotation_group="GGCGCGCC"
                     /label="AscI"
                     /note="Geneious type: restriction site"
     misc_feature    2523..2530
                     /Recognition_pattern="GG^CGCGCC"
                     /annotation_group="GGCGCGCC"
                     /label="AscI, PalAI, SgsI"
                     /note="Geneious type: restriction site"
     protein_bind    complement(2531..2564)
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
                     /label="loxP"
     protein_bind    complement(2615..2648)
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GGATACTT). lox2272 sites are compatible with
                     eachother, but incompatible with loxP or loxN sites."
                     /label="lox2272"
     misc_feature    2661..2666
                     /Recognition_pattern="T^CTAGA"
                     /annotation_group="TCTAGA"
                     /label="XbaI"
                     /note="Geneious type: restriction site"
     misc_feature    2667..2672
                     /Recognition_pattern="G^GATCC"
                     /annotation_group="GGATCC"
                     /label="BamHI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(2705..2724)
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
                     /label="pCAG-F"
     primer_bind     complement(2741..2763)
                     /note="Chimeric intron in CAG promoter, forward primer"
                     /label="pCAGGS-5"
     misc_feature    2836..2841
                     /Recognition_pattern="G^GGCCC"
                     /annotation_group="GGGCCC"
                     /label="Bsp120I, PspOMI"
                     /note="Geneious type: restriction site"
     misc_feature    2836..2841
                     /Recognition_pattern="GGGCC^C"
                     /annotation_group="GGGCCC"
                     /label="ApaI"
                     /note="Geneious type: restriction site"
     misc_feature    2903..2908
                     /Recognition_pattern="AGC^GCT"
                     /annotation_group="AGCGCT"
                     /label="Eco47III, AfeI, Aor51HI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(3019..3031)
                     /Recognition_pattern="(8/13)GACNNNNNNTTYG(11/6)"
                     /annotation_group="GACNNNNNNTTYG"
                     /label="ArsI"
                     /note="Geneious type: restriction site"
     promoter        complement(3043..3320)
                     /label="chicken beta-actin promoter"
     misc_feature    3448..3453
                     /Recognition_pattern="CA^TATG"
                     /annotation_group="CATATG"
                     /label="NdeI, FauNDI"
                     /note="Geneious type: restriction site"
     repeat_region   complement(3631..3760)
                     /label="AAV2 ITR"
     primer_bind     complement(3677..3688)
                     /note="L4440 vector, forward primer"
                     /label="L4440"
     primer_bind     3738..3749
                     /note="L4440 vector, forward primer"
                     /label="L4440"
     rep_origin      3832..4287
                     /direction="LEFT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     complement(3919..3938)
                     /note="F1 origin, reverse primer"
                     /label="F1ori-R"
     misc_feature    4060..4068
                     /Recognition_pattern="CACNNN^GTG"
                     /annotation_group="CACNNNGTG"
                     /label="DraIII, AdeI"
                     /note="Geneious type: restriction site"
     primer_bind     4129..4150
                     /note="F1 origin, forward primer"
                     /label="F1ori-F"
     misc_feature    4282..4293
                     /Recognition_pattern="(11/13)CAANNNNNGTGG(12/10)"
                     /annotation_group="CAANNNNNGTGG"
                     /label="CspCI"
                     /note="Geneious type: restriction site"
     promoter        4313..4417
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             4418..5278
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     primer_bind     complement(4636..4655)
                     /note="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     misc_feature    4722..4727
                     /Recognition_pattern="AGT^ACT"
                     /annotation_group="AGTACT"
                     /label="ScaI, BmcAI, ZrmI"
                     /note="Geneious type: restriction site"
     rep_origin      5449..6037
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     5938..5957
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     misc_feature    6093..6098
                     /Recognition_pattern="A^CATGT"
                     /annotation_group="ACATGT"
                     /label="PciI, PscI"
                     /note="Geneious type: restriction site"
     misc_feature    6093..6098
                     /Recognition_pattern="RCATG^Y"
                     /annotation_group="RCATGY"
                     /label="NspI, BstNSI, XceI"
                     /note="Geneious type: restriction site"
     primer_bind     6191..6208
                     /note="L4440 vector, forward primer"
                     /label="L4440"
ORIGIN      
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg
       61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg
      121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
      181 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa
      241 gatccttatc gattttacca catttgtaga ggttttactt gctttaaaaa acctcccaca
      301 tctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
      361 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      421 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgctc
      481 gaagcggccg cccgggtcga cggtatcgat gcggggaggc ggcccaaagg gagatccgac
      541 tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg
      601 aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa
      661 tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca
      721 cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga
      781 tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg
      841 tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac
      901 gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg
      961 aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt
     1021 atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt
     1081 tgtaatccag aggttgatta tcgataagct tgatatcgaa ttcataactt cgtatagcat
     1141 acattatacg aagttattct ttgcaccatt ctaaagaata acagtgataa tttctgggtt
     1201 aaggcaaata acttcgtata ggatacttta tacgaagtta tgctagcggg agacccaagc
     1261 ttaccaccat gtacccatac gatgttcctg actatgcgga ctacaaagac gatgacgaca
     1321 agaagtacct ggaggaccgg ggcgaggtga cttttgagaa gatcttctcc cagaagctgg
     1381 ggtacctgct tttccgagac ttctgcctga agcacctgga ggaggccaag cccttggtag
     1441 agttctacga ggagatcaag aaatacgaga agctggagac agaggaggag cgcctggtct
     1501 gcagccgaga gatcttcgac acgtacatca tgaaggagct gctggcctgc tcacatcctt
     1561 tctcgaagag cgccattgag cacgtccagg gccatctggt gaagaagcag gtgcctccgg
     1621 atctcttcca gccatatatt gaagaaattt gccagaacct ccgaggagac gtgttccaga
     1681 aattcatcga gagcgataaa ttcacacgat tttgccagtg gaagaatgta gagctcaaca
     1741 tccacggtag tggcgcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga
     1801 atcctggacc gatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     1861 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     1921 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     1981 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     2041 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     2101 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     2161 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     2221 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     2281 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     2341 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     2401 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     2461 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     2521 agggcgcgcc ataacttcgt ataatgtatg ctatacgaag ttattaagag gtttcatatt
     2581 gctaatagca gctacaatcc agctaccatt ctgcataact tcgtataaag tatcctatac
     2641 gaagttattc cggagtcgac tctagaggat cccgatccaa ttctttgcca aaatgatgag
     2701 acagcacaat aaccagcacg ttgcccagga gagagccgcc ggtcacacgc cagaagccga
     2761 accccgccct gccccgtccc ccccgaaggc agccgtcccc ccgcggacag ccccgagccg
     2821 ctccccccgc acaaagggcc ctcccggagc ccctcaaggc tttcacgcag ccacagaaaa
     2881 gaaacaagcc gtcattaaac caagcgctaa ttacagcccg gaggagaagg gccgtcccgc
     2941 ccgctcacct gtgggagtaa cgcggtcagt cagagccggg gcgggcggcg cgaggcggcg
     3001 gcggagcggg gcacggggcg aaggcagcgt cgcagcgact cccgcccgcc gcgcgcttcg
     3061 ctttttatag ggccgccgcc gccgccgcct cgccataaaa ggaaactttc ggagcgcgcc
     3121 gctctgattg gctgccgccg cacctctccg cctcgccccg ccccgcccct cgccccgccc
     3181 cgccccgcct ggcgcgcgcc cccccccccc ccccgccccc atcgctgcac aaaataatta
     3241 aaaaataaat aaatacaaaa ttgggggtgg ggaggggggg gagatgggga gagtgaagca
     3301 gaacgtgggg ctcacctcga ccatggtaat agcgatgact aatacgtaga tgtactgcca
     3361 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
     3421 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
     3481 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
     3541 gggaacatac gtcattattg acgtcaatgg tactcttcct agagcatggc tacgtagata
     3601 agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc
     3661 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     3721 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctggcgtaat agcgaagagg
     3781 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     3841 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3901 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3961 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4021 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4081 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4141 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4201 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4261 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     4321 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4381 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4441 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4501 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4561 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4621 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4681 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4741 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4801 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4861 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4921 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4981 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5041 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     5101 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     5161 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     5221 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5281 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5341 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5401 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5461 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5521 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5581 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5641 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5701 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5761 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5821 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5881 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5941 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6001 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6061 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     6121 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     6181 gaccgagcgc agcgagtcag tgagcgagga agcggaag
//
