LOCUS       Exported                5517 bp ds-DNA     circular SYN 21-APR-2017
DEFINITION  GFP-X-FRB-E-Rit CT.
ACCESSION   .
VERSION     .
KEYWORDS    GFP-MAPPER
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 14, 2018 from SnapGene Viewer 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="GFP-X-FRB-E-Rit CT"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     promoter        17..569
                     /label=CMV_immearly_promoter
                     /note="color: #ffffff"
     CDS             607..2175
                     /codon_start=1
                     /gene="EGFP"
                     /label=EGFP-MAPPER
                     /label=EGFP
                     /note="ORF frame 1"
                     /note="color: #------"
                     /translation="MDVCVRLALWLLWGLLLHQGQSLAPVATMVSKGEELFTGVVPILV
                     ELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYP
                     DHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDG
                     NILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPV
                     LLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGSTSLDTVLFGPP
                     LLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHGAGAGAGAGAGALEEAAAREAA
                     AREAAAREAAARVEILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISKSAGGSAGGSAG
                     GRILEAAAREAAAREAAAREAAAREAAAREAAARAILMEKKSKPKNSVWKRLKSPFRKK
                     KDSVT"
     misc_feature    607..690
                     /label=ER signal peptide
                     /note="color: #a6acb3"
     misc_feature    685..690
                     /label=Kozak seq
                     /note="color: #------"
     CDS             691..1407
                     /codon_start=1
                     /label=EGFP
                     /note="color: #00ff00"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1417..2175
                     /codon_start=1
                     /label=MAPPER
                     /note="color: #ff9900"
                     /translation="TSLDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYS
                     KEHGAGAGAGAGAGALEEAAAREAAAREAAAREAAARVEILWHEMWHEGLEEASRLYFG
                     ERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQ
                     AWDLYYHVFRRISKSAGGSAGGSAGGRILEAAAREAAAREAAAREAAAREAAAREAAAR
                     AILMEKKSKPKNSVWKRLKSPFRKKKDSVT"
     misc_feature    1669..1947
                     /label=FRB
                     /note="color: #a6acb3; direction: RIGHT"
     terminator      2198..2426
                     /label=SV40_PA_terminator
                     /note="color: #ffffff"
     rep_origin      complement(2565..2871)
                     /direction=LEFT
                     /label=f1_origin
                     /note="color: #ffff00"
     promoter        2950..2978
                     /label=AmpR_promoter
                     /note="color: #ffffff"
     promoter        3062..3330
                     /label=SV40_promoter
                     /note="color: #ffffff"
     CDS             3413..4207
                     /codon_start=1
                     /gene="NeoR/KanR"
                     /label=NeoR/KanR
                     /note="ORF frame 2"
                     /note="color: #993366"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      4382..4651
                     /label=TK_PA_terminator
                     /note="color: #ffffff"
     rep_origin      4799..5418
                     /label=pBR322_origin
                     /note="color: #ffff00"
ORIGIN
        1 cataagttag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
       61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      601 agcaccatgg atgtatgcgt ccgtcttgcc ctgtggctcc tctggggact cctcctgcac
      661 cagggccaga gcctcgcacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
      721 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      781 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      841 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
      901 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      961 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1021 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1081 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1141 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     1201 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1261 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     1321 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1381 actctcggca tggacgagct gtacaagtcc ggatcaacta gtctggatac agtgctcttt
     1441 gggcctcctc tcttgactcg ccataatcac ctcaaggact tcatgctggt ggtgtctatc
     1501 gttattggtg tgggcggctg ctggtttgcc tatatccaga accgttactc caaggagcac
     1561 ggagcgggcg ctggtgccgg agctggagca ggtgctctcg aggaagcagc agcacgagaa
     1621 gctgctgcta gagaagctgc tgctagagaa gcagcagcac gagtcgagat cctctggcat
     1681 gagatgtggc atgaaggcct ggaagaggca tctcgtttgt actttgggga aaggaacgtg
     1741 aaaggcatgt ttgaggtgct ggagcccttg catgctatga tggaacgggg cccccagact
     1801 ctgaaggaaa catcctttaa tcaggcctat ggtcgagatt taatggaggc ccaagagtgg
     1861 tgcaggaagt acatgaaatc agggaatgtc aaggacctcc tccaagcctg ggacctctat
     1921 tatcatgtgt tccgacgaat ctcaaagtca gctggaggca gtgcaggtgg ctctgccgga
     1981 ggtagaattc tagaagctgc tgctagagaa gctgctgcta gagaagctgc tgctagagaa
     2041 gctgctgcta gagaagctgc tgctagagaa gctgctgcta gagcaattct aatggagaaa
     2101 aaatctaagc ccaaaaacag tgtatggaag aggctaaaat caccattccg gaagaagaaa
     2161 gattcagtaa cttgaggatc caccggatct agataactga tcataatcag ccataccaca
     2221 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     2281 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     2341 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     2401 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa
     2461 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     2521 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     2581 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     2641 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     2701 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     2761 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     2821 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     2881 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2941 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3001 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     3061 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3121 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3181 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3241 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     3301 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     3361 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     3421 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     3481 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     3541 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     3601 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     3661 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     3721 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     3781 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     3841 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     3901 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     3961 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     4021 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     4081 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     4141 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     4201 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     4261 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     4321 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     4381 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     4441 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     4501 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     4561 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     4621 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     4681 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     4741 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     4801 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     4861 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     4921 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     4981 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5041 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5101 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5161 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5221 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5281 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5341 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5401 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     5461 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
