LOCUS       pNBU2_erm_TetR_P        6362 bp ds-DNA     linear       15-MAY-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2531..3811
                     /label=NBU2 Integrase ORF
                     /ApEinfo_fwdcolor="#50ff41"
                     /ApEinfo_revcolor="#50ff41"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1802..2317
                     /label=NanoLuc reporter gene
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1498..1501)
                     /label=-33 binding site
                     /ApEinfo_fwdcolor="#ff764f"
                     /ApEinfo_revcolor="#ff764f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1714..1717
                     /label=-33 binding site(1)
                     /ApEinfo_label="-33 binding site"
                     /ApEinfo_fwdcolor="#ff764f"
                     /ApEinfo_revcolor="#ff764f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3971..3983
                     /label=attN2
                     /ApEinfo_fwdcolor="#ff786c"
                     /ApEinfo_revcolor="#ff786c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1739..1746
                     /label=-7 binding site
                     /ApEinfo_fwdcolor="#ff7e51"
                     /ApEinfo_revcolor="#ff7e51"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1464..1465)
                     /label=+1 txn intiation site
                     /ApEinfo_fwdcolor="#ffbd5c"
                     /ApEinfo_revcolor="#ffbd5c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1751..1751
                     /label=+1 txn initiation site
                     /ApEinfo_fwdcolor="#ffd544"
                     /ApEinfo_revcolor="#ffd544"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5473..6334
                     /label=bla ORF
                     /ApEinfo_fwdcolor="#6b92ff"
                     /ApEinfo_revcolor="#6b92ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1752..1770
                     /label=tetR binding site
                     /ApEinfo_fwdcolor="#ffc061"
                     /ApEinfo_revcolor="#ffc061"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1470..1477)
                     /label=-7 binding site(1)
                     /ApEinfo_label="-7 binding site"
                     /ApEinfo_fwdcolor="#ff511e"
                     /ApEinfo_revcolor="#ff511e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    10..795
                     /label=RP4 oriT/R6K oriV
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1421..1463)
                     /label=A21 RBS
                     /ApEinfo_fwdcolor="#fd32ff"
                     /ApEinfo_revcolor="#fd32ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2447..2470
                     /label=3' sequencing primer
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(797..1420)
                     /label=tetR
                     /ApEinfo_fwdcolor="#fc1f14"
                     /ApEinfo_revcolor="#fc1f14"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1610..1630
                     /label=5' sequencing primer
                     /ApEinfo_fwdcolor="#f958ff"
                     /ApEinfo_revcolor="#f958ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4251..4985
                     /label=ermG ORF
                     /ApEinfo_fwdcolor="#82ff8f"
                     /ApEinfo_revcolor="#82ff8f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(1690..1708)
                     /label=tetR binding site(1)
                     /ApEinfo_label="tetR binding site"
                     /ApEinfo_fwdcolor="#ffc061"
                     /ApEinfo_revcolor="#ffc061"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1772..1790
                     /label=GH023 ribosome binding site
                     /ApEinfo_fwdcolor="#97eaff"
                     /ApEinfo_revcolor="#97eaff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 CAATTCGAGG GGGATCAATT CCGTGATAGG TGGGCTGCCC TTCCTGGTTG GCTTGGTTTC
       61 ATCAGCCATC CGCTTGCCCT CATCTGTTAC GCCGGCGGTA GCCGGCCAGC CTCGCAGAGC
      121 AGGATTCCCG TTGAGCACCG CCAGGTGCGA ATAAGGGACA GTGAAGAAGG AACACCCGCT
      181 CGCGGGTGGG CCTACTTCAC CTATCCTGCC CGGCTGACGC CGTTGGATAC ACCAAGGAAA
      241 GTCTACACGA ACCCTTTGGC AAAATCCTGT ATATCGTGCG AAAAAGGATG GATATACCGA
      301 AAAAATCGCT ATAATGACCC CGAAGCAGGG TTATGCAGCG GAAAACGGAA TTGATCCGGC
      361 CACGATGCGT CCGGCGTAGA GGATCTGAAG ATCAGCAGTT CAACCTGTTG ATAGTACGTA
      421 CTAAGCTCTC ATGTTTCACG TACTAAGCTC TCATGTTTAA CGTACTAAGC TCTCATGTTT
      481 AACGAACTAA ACCCTCATGG CTAACGTACT AAGCTCTCAT GGCTAACGTA CTAAGCTCTC
      541 ATGTTTCACG TACTAAGCTC TCATGTTTGA ACAATAAAAT TAATATAAAT CAGCAACTTA
      601 AATAGCCTCT AAGGTTTTAA GTTTTATAAG AAAAAAAAGA ATATATAAGG CTTTTAAAGC
      661 TTTTAAGGTT TAACGGTTGT GGACaacaag ccagggatgt aacgCACTGA GAAGCCCTTA
      721 GAGCCTCTCA AAGCAATTTT GAGTGACACA GGAACACTTA ACGGCTGACA TGGGAATTCC
      781 CCTCCACCGC GGTGGCTTAA GACCCACTTT CACATTTAAG TTGTTTTTCT AATCCGCATA
      841 TGATCAATTC AAGGCCGAAT AAGAAGGCTG GCTCTGCACC TTGGTGATCA AATAATTCGA
      901 TAGCTTGTCG TAATAATGGC GGCATACTAT CAGTAGTAGG TGTTTCCCTT TCTTCTTTAG
      961 CGACTTGATG CTCTTGATCT TCCAATACGC AACCTAAAGT AAAATGCCCC ACAGCGCTGA
     1021 GTGCATATAA TGCATTCTCT AGTGAAAAAC CTTGTTGGCA TAAAAAGGCT AATTGATTTT
     1081 CGAGAGTTTC ATACTGTTTT TCTGTAGGCC GTGTACCTAA ATGTACTTTT GCTCCATCGC
     1141 GATGACTTAG TAAAGCACAT CTAAAACTTT TAGCGTTATT ACGTAAAAAA TCTTGCCAGC
     1201 TTTCCCCTTC TAAAGGGCAA AAGTGAGTAT GGTGCCTATC TAACATCTCA ATGGCTAAGG
     1261 CGTCGAGCAA AGCCCGCTTA TTTTTTACAT GCCAATACAA TGTAGGCTGC TCTACACCTA
     1321 GCTTCTGGGC GAGTTTACGG GTTGTTAAAC CTTCGATTCC GACCTCATTA AGCAGCTCTA
     1381 ATGCGCTGTT AATCACTTTA CTTTTATCTA ATCTAGACAT attcgtttaa tatcataaat
     1441 aatttatttt attttaaaat gcgCGggtgC AAAGGTAaga ggttttattt taactacCAA
     1501 Atgttttcgg aagttttttc gcttttcttt ttctatcgtt tctcagactc tcttagcgaa
     1561 agggaaagaa ggtaaagaag aaaaacaaaa cgccttttct tTtttgcacc cgctttccaa
     1621 gagaagaaag ccttgttaaa ttgacttagt gtaaaagcgc agtactgctt gaccataaga
     1681 acaaaaaaaT CTCTATCACT GATAGGGAta aagTTTGgaa gataaagcta aaagttctTA
     1741 TCTTTGcagt CTCCCTATCA GTGATAGAGA cGAAATAAAG ACATATAAAA gaaaagacac
     1801 cATGGTCTTC ACACTCGAAG ATTTCGTTGG GGACTGGCGA CAGACAGCCG GCTACAACCT
     1861 GGACCAAGTC CTTGAACAGG GAGGTGTGTC CAGTTTGTTT CAGAATCTCG GGGTGTCCGT
     1921 AACTCCGATC CAAAGGATTG TCCTGAGCGG TGAAAATGGG CTGAAGATCG ACATCCATGT
     1981 CATCATCCCG TATGAAGGTC TGAGCGGCGA CCAAATGGGC CAGATCGAAA AAATTTTTAA
     2041 GGTGGTGTAC CCTGTGGATG ATCATCACTT TAAGGTGATC ctgcactatg gcacactggt
     2101 AATCGACGGG GTTACGCCGA ACATGATCGA CTATTTCGGA CGGCCGTATG AAGGCATCGC
     2161 CGTGTTCGAC GGCAAAAAGA TCACTGTAAC AGGGACCCTG TGGAACGGCA acaaaattat
     2221 cgacgagcgc CTGATCAACC CCGACGGCTC CCTGCTGTTC CGAGTAACCA TCAACGGAGT
     2281 GACCGGCTGG CGGCTGTGCG AACGCATTCT GGCGTAACTA ATTGCCTATC TTCCAGTGAT
     2341 GGAACAGCAT TTGTGCATTG GCTGCAACAA TCAGCCTTAC TTGTGCCTGT TCTATTTCCG
     2401 AACCGACCGC TTGTATGAAT CCATCAAAAT TCGTTTTCTC TATGTTGGAT TCCTTGTTGC
     2461 TCATATTGTG ATGATAATTT CTACAAATAT AGTCATTGGT AACTATCTAT GAAACTGTTT
     2521 GATACTTTTA TAGTTGATTA AACTTGTTCA TGGCATTTGC CTTAATATCA TCCGCTATGT
     2581 CAATGTAGGG TTTCATAGCT TTGTAGTCGC TGTGTCCCGT CCATTTCATG ACCACCTGTG
     2641 CCGGGATTCC GAGAGCCAGC GCATTGCAGA TGAATGTCCT TCTTCCTGCA TGGGTACTGA
     2701 GCAAAGCGTA TTTGGGTGTG ACTTCATCAA TACGTTCATT TCCCTTGTAG TAGGTTTCCC
     2761 GTACAGGCTC GTTGATTTCT GCCAGTTCGC CCAGCTCTTT CAGGTAATCG TTCATCTTCT
     2821 GGTTGCTGAT GACGGGCAGA GCCATGTAAT TCTCGAAATG GATGTCCTTG TATTTGTCCA
     2881 GTATGGCTTT GCTGTATTTG TTCAGTTCAA TCGTCAGGCT GTCGGCAGTC TTGACTGTGG
     2941 TTATTTCGAT GTGGTCGGAC TTCACATCGC TTCTTTTCAG ATTGCGAACA TCCGAATACC
     3001 GCAAACTCGT AAAGCAGCAG AACAGGAAAA CATCACGCAC ACGTTCCAGG TATTGCTTAT
     3061 CCTTGGGTAT CTGGTAGTCT TTCAGCTTGT TCAGTTCATC CCAAGTCAGG AAGATTACTT
     3121 TTTTCGAGGT GGTTTTCAGT TTCGGTTTGA ACGTATCGTA TGCAATGTTC TGATGATGTC
     3181 CTTTCTTGAA GCTCCAGCGC AGGAACCATT TGAGGAATCC CATTTGCTTG CCGATGGTGC
     3241 TGTTTCTCAT ATCCTTGGTG TCACGCAGGA AGTTGACGTA TTCGTTCAAT CCAAACTCGT
     3301 TGAAATAGTT GAACGTTGCA TCCTCCTTGA ACTCTTTGAG GTGGTTCCTC ACTGCTGCAA
     3361 ATTTTTCATA GGTGGATGCC GTCCAGTTAT TCTGGTTACC GCACTCTTTT ACAAACTCAT
     3421 CGAACACCTC CCAAAAGCTG ACAGGGGCTT CTTCCGGCTG TTCTTCGCTG GTGTCTTTCA
     3481 TTCTCATGTT GAAAGCTTCC TTCAACTGTT GGGTCGTTGG CATGACCTCC TGCACCTCAA
     3541 ATTCCTTGAA AATATTCTGG ATTTCGGCAT AGTATTTCAG CAAGTCCGTA TTGATTTCGG
     3601 CTGCACTTTG CTTTAGCTTG TTGGTACATC CGCTCTTTAC CCGCTGCTTA TCTGCATCCC
     3661 ATTTGGCTAC GTCAATCCGG TAGCCCGTTG TAAACTCGAT GCGTTGGCTG GCAAAGATGA
     3721 CACGCATACG GATGGGTACG TTCTCTACGA TTGGCACACC GTTCTTTTTC CGGCTCTCCA
     3781 ATGCAAAAAT GATGTTGCGC TTGATATTCA TAATTGGGTG CGTTTGAAAT TCTACACCCA
     3841 AATATACACC CAATTATTGA GATAGCAAAA GACATTTAGA AACATTTACT TTTACTCTAT
     3901 ATTGTAATTT ACACTTGATT ATCAGTCGTT TGCAGTCTTA TGATATTCTG TGAAAGTATA
     3961 AGTTCGAGAG CCTGTCTCTC CGCAAAAAAC GCTGAAAATC AGCAGATTGC AAAACAAACA
     4021 CCCTGTTTTA CACCCAAGAA TGTAAAGTCG GCTGTTTTTG TTTTATTTAA GATAATACAA
     4081 CCACTACATA ATAAAAGAGT AGCGATATTA AAAGAATCCG ATGAGAAAAG ACTAATATTT
     4141 ATCTATCCAT TCAGTTTGAT TTTTCAGGAC TTTACATCGT CCTGAAAGTA TTTGTTGGTA
     4201 CCGGTACCGA GGACGCGtaa acatttacag ttgcatgtgg cctattgttt TTAGCCGTTA
     4261 AATATTTTAT AACTATTAAA TAGCGATACA AATTGTTCGA AACTAATATT GTTTATATCA
     4321 TATATTCTCG CATGTTTTAA AGCTTTATTA AATTGATTTT TTGTAAACAG TTTTTCGTAC
     4381 TCTTTGTTAA CCCATTTCAT TACAAAAGTT TCATATTTTT TTCTCTCTTT AAATGCCATT
     4441 TTTGCTGGCT TTCTTTTTAA TACAATTAAT GTGCTATCCA CTTTAGGTTT TGGATGGAAA
     4501 TAATACCTAG GAATTTTTGC TAATATAGAA ATATCTACCT CTGCCATTAA CAGCAATGCT
     4561 AGTGATCTGT TTGTATCTAA TAACATTTTA GCAAAACCAT ATTCCACTAT TAAATAACTT
     4621 ATTGTGGCTG AACTTTCAAA AACAATTTTT CGAATTATAT TTGTGCTTAT GTTGTAAGGT
     4681 ATGCTGCCAA ATATTTTATA TGGATTGTGG CTAGGAAATG TAAATTTCAG TATATCATCA
     4741 TTTACTATTT GATAGTTAGG ATAATTTAAG AGCTTATTAC GAGTTACCTC ACATAATTTA
     4801 GAATCAATTT CTATCGCCGT TACAAAATTA CATCTCTTTA CCAATCCAGC AGTAAAATGA
     4861 CCTTTCCCTG CACCTATTTC AAAGATGTTA TCTTTTTCAT CTAAACTTAT GCAATTCATT
     4921 ATTTTTTCTA TGTGATATTT TGAAGTAATA AAATTTTGAC TATCTTTTAT ATTTACTTTG
     4981 TTCATTATAA CCTCTCCTTA ATTTATTGCA TCTCTTTTCG AATATTTATG TTTTTTGAGA
     5041 AAAGAACGTA CTCATGGTTC ATCCCGATAT GCGTATCGGT CTGTATATCA GCAACTTTCT
     5101 ATGTGTTTCA ACTACAATAG TCATCTATTC TCATCTTTCT GAGTCCACCC CCTGCAAAGC
     5161 CCCTCtttac gacataaaaa ttcggtCGGA AAAGGTATGC AAAAGATGTT TCTCTCTTTA
     5221 AGAGAAACTC TTCGGGATGC AAAAATATGA AAATAACTCC AATTCACCAA ATTATATAGC
     5281 GACTTTTTTA CAAAATGCTA AAATTTGTTG ATTTCCGTCA AGCAATTGTT GAGCAAAAAT
     5341 GTCTTTTACG ATAAAATGAT ACCTCAATAT CAACTGTTTA GCAAAACGAT ATTTCTCTTA
     5401 AAGAGAGAAA CACCTTTTTG TTCACCAATC CCCGACTTTT AATCCCGCGG CCATGATTGA
     5461 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA
     5521 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT
     5581 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG
     5641 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC
     5701 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT
     5761 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA
     5821 GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT
     5881 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT
     5941 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT
     6001 GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA
     6061 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA
     6121 CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT
     6181 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC
     6241 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT
     6301 GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATAACGC
     6361 GT     
//
