LOCUS       pPRISM_TagRFP_cmlc2_eGFP        6893 bp ds-DNA     circular     11-SEP-2018
DEFINITION  synthetic circular DNA, gene-targeting cassette
ACCESSION   .
VERSION     2.0
KEYWORDS    pPRISM, TagRFP
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Jordan M Welker,  Maira P Almeida, Melanie Torrie, Wesley A Wierson, Carla M Mann, Trevor Weiss, Cassandra Bullard, Katherine Knudtson, Kenna McKeighan, Stephen C. Ekker, Drena L Dobbs, Maura McGrail, Karl J Clark, Jeffrey J Essner
  TITLE     pPRISM an expanded tool set for precise genome editing
  JOURNAL   .
COMMENT     
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     misc_feature    complement(267..457)
                     /note="ITR(R)"
                     /note="Tol2 ITR(R)"
                     /locus_tag="ITR(R)"
                     /label="ITR(R)"
                     /ApEinfo_label="ITR(R)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="#80ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5080..5102)
                     /locus_tag="3' Universal Guide (w/PAM)"
                     /label="3' Universal Guide (w/PAM)"
                     /ApEinfo_label="3' Universal Guide (w/PAM)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             994..1710
                     /codon_start=2
                     /product="monomeric derivative of red fluorescent protein
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /note="TagRFP"
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     R
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     V
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     M
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     A RYCDLPSKLGHKLN"
                     /locus_tag="TagRFP, monomeric red fluorescent protein"
                     /label="TagRFP, monomeric red fluorescent protein"
                     /ApEinfo_label="TagRFP, monomeric red fluorescent protein"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             937..993
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /locus_tag="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label="2A peptide from porcine teschovirus-1 polyprotein"
                     /ApEinfo_label="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /ApEinfo_fwdcolor="#00ff80"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    1719..2894
                     /note="ocean pout terminator"
                     /note="3'UTR, poly(A) signal, and putative boundary
                     element from ocean pout (FRM- Pat Gibbs)."
                     /locus_tag="ocean pout terminator"
                     /label="ocean pout terminator"
                     /ApEinfo_label="ocean pout terminator"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     3467..3496
                     /note="NLS"
                     /locus_tag="NLS"
                     /label="NLS"
                     /ApEinfo_label="NLS"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    4221..4445
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
                     /locus_tag="bGH poly(A) signal"
                     /label="bGH poly(A) signal"
                     /ApEinfo_label="bGH poly(A) signal"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3497..4216
                     /vntifkey="4"
                     /locus_tag="eGFP"
                     /label="eGFP"
                     /ApEinfo_label="eGFP"
                     /ApEinfo_fwdcolor="#00ff0e"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    4482..4686
                     /note="SV40(A)"
                     /note="SV40"
                     /locus_tag="SV40(A)"
                     /label="SV40(A)"
                     /ApEinfo_label="SV40(A)"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    4687..4891
                     /note="SV40(A)"
                     /note="SV40"
                     /locus_tag="SV40(A)(1)"
                     /label="SV40(A)(1)"
                     /ApEinfo_label="SV40(A)"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5144..5358
                     /note="ITR(L)"
                     /note="Tol2 ITR(L)"
                     /locus_tag="ITR(L)"
                     /label="ITR(L)"
                     /ApEinfo_label="ITR(L)"
                     /ApEinfo_fwdcolor="#80ffff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             5423..6232
                     /note="Kan(R)"
                     /locus_tag="Kan(R)"
                     /label="Kan(R)"
                     /ApEinfo_label="Kan(R)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    918..923
                     /locus_tag="BfuAI site 2"
                     /label="BfuAI site 2"
                     /ApEinfo_label="BfuAI site 2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(6265..6893)
                     /direction=LEFT
                     /note="ColE1 Ori"
                     /locus_tag="ColE1 Ori"
                     /label="ColE1 Ori"
                     /ApEinfo_label="ColE1 Ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    928..931
                     /locus_tag="BfuAI site 2 overhang"
                     /label="BfuAI site 2 overhang"
                     /ApEinfo_label="BfuAI site 2 overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1..253)
                     /direction=LEFT
                     /note="ColE1 Ori"
                     /locus_tag="ColE1 Ori(1)"
                     /label="ColE1 Ori(1)"
                     /ApEinfo_label="ColE1 Ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(514..519)
                     /locus_tag="BfuAI site 1"
                     /label="BfuAI site 1"
                     /ApEinfo_label="BfuAI site 1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          3240..3446
                     /note="mIntron"
                     /note="carp bactin shortened intron I"
                     /locus_tag="mIntron"
                     /label="mIntron"
                     /ApEinfo_label="mIntron"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(506..509)
                     /locus_tag="BfuAI site 1 overhang"
                     /label="BfuAI site 1 overhang"
                     /ApEinfo_label="BfuAI site 1 overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    487..509
                     /locus_tag="5' Universal Guide (w/PAM)"
                     /label="5' Universal Guide (w/PAM)"
                     /ApEinfo_label="5' Universal Guide (w/PAM)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    642..917
                     /vntifkey="21"
                     /locus_tag="lacZ'"
                     /label="lacZ'"
                     /ApEinfo_label="lacZ'"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="#1dda7b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        520..641
                     /vntifkey="30"
                     /locus_tag="lacZ\promoter"
                     /label="lacZ\promoter"
                     /ApEinfo_label="lacZ\promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          4981..5069
                     /note="Carp beta-actin intron 1 filler"
                     /locus_tag="Carp beta-actin intron 1 filler"
                     /label="Carp beta-actin intron 1 filler"
                     /ApEinfo_label="Carp beta-actin intron 1 filler"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5072..5078
                     /locus_tag="BspQI site 2"
                     /label="BspQI site 2"
                     /ApEinfo_label="BspQI site 2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2921..3195
                     /note="cmlc2 enhancer/promoter xu lab"
                     /locus_tag="cmlc2 enhancer/promoter xu lab"
                     /label="cmlc2 enhancer/promoter xu lab"
                     /ApEinfo_label="cmlc2 enhancer/promoter xu lab"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4966..4972)
                     /locus_tag="BspQI site 1"
                     /label="BspQI site 1"
                     /ApEinfo_label="BspQI site 1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4962..4964)
                     /locus_tag="BspQI site 1 overhang"
                     /label="BspQI site 1 overhang"
                     /ApEinfo_label="BspQI site 1 overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5080..5082
                     /locus_tag="BspQI site 2 overhang"
                     /label="BspQI site 2 overhang"
                     /ApEinfo_label="BspQI site 2 overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
       61 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      121 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
      181 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      241 ggaagcggaa cagatttaaa tggtaccgag cagaggtgta aaaagtactc aaaaatttta
      301 ctcaagtgaa agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg
      361 gctagaatct tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt
      421 aaggaagtaa aagtaaaagc aagaaagaaa actagagtgg tctcccttta gtgagggtca
      481 attgatGGGA GGCGTTCGGG CCACAGCGGa cacgcaggtg cgcaacgcaa ttaatgtgag
      541 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      601 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      661 gcgcgcaatt aaccctcact aaagggaaca aaagctggag gacctcgagg gggggcccgg
      721 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
      781 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      841 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      901 gcctgaatgg cgaatggacc tgccacactt cccggagcca cgaacttctc tctgttaaag
      961 caagcaggag acgtggaaga aaaccccggt cctctcgagg tgtctaaggg cgaggaactg
     1021 attaaggaga acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc
     1081 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
     1141 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac
     1201 ggcagcagaa ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc
     1261 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
     1321 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
     1381 aacttcccat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccaacacc
     1441 gagatgctgt accccgctga cggcggcctg gaaggcagaa gcgacatggc cctgaagctc
     1501 gtgggcgggg gccacctgat ctgcaacttc aagaccacat acagatccaa gaaacccgct
     1561 aagaacctca agatgcccgg cgtctactat gtggaccaca gactggaaag aatcaaggag
     1621 gccgacaaag agacctacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc
     1681 cctagcaaac tggggcacaa acttaattga aactagtaat taagtctcag ccaccgttaa
     1741 ctgaacatgt caaaacctgt ggagactgtt gagatttgat gttctgaaaa gataaagcct
     1801 ataaataaaa tgttgcccaa atttcctgcc tgatgttttt ctttgtcttt gctacatggc
     1861 tttgctgctc ggatcggctc actctgtgta tgccacgttc actttgtact ctccttctca
     1921 cggtaggttt attattttta gatgtgcagt tagtttctgt gaaataacac accacacact
     1981 gatattgtct gtgcattgac ttggtgagtg cacattgttt ttgatcttga catatttata
     2041 tttgattgat caggtgaact gtgtgaatct aaagtgctcc atacagatgt tctgcattga
     2101 aaatattctc attttattag tggaagtgag tgtatgccac atccaatcaa tttcagcaaa
     2161 caccccagta tgatttaatg caaaaaaatg aaggtatcaa acacgcatta ctactttgca
     2221 gttaaatatt taacatttat tccaacacga aaaaaagcag taaataacac tttgacaaac
     2281 acgtcaggac atcttatttt tgtcaccctc acaggcaatt tagtataata tattatatat
     2341 atatatatat catataataa tattcagtat aatatatata tatatatcat attataatat
     2401 tcagtataat ataaaacaca aacacatata tgtataatat aatataacat ttttatttat
     2461 tgagatgcct ctatggaccg tgttataaga agtaaagatc aggagaagta aacatgaagt
     2521 gtaattatga atactgatgt taaattaagc tatgatgagt tttcactgtt aatttaccat
     2581 ctcaattaaa tgttgatgcc tccatgacca agttaagcag atgagactga gacaactgta
     2641 gaagacaaga tgttcacttt gctgaatata gctggcttga cagttatcta tgactctata
     2701 aatatatata tatttttttt tttataaaat gatttattta taactatata tccatttctc
     2761 agacaggtgc ttcatatccc tcactcccgt agctgtccat gctggatctg tccccgttgt
     2821 ttttaaaaag ctaaataagt tattaacatg actgcatcca gcgagccaaa cctgtctggt
     2881 gtacagctac cagagaagct tgagatccta gtcaccggcg gcattcatcc atccttttca
     2941 tccctcaaat ctctcattca cgtccccctc cccatctgca cactttatct cattttccac
     3001 cctgctggaa tctgagcact tgtgcagtta tcagggctcc tgtatttagg aggctctggg
     3061 tgtccatgta ggggacgaac agaaacactg cagaccttta tagaagaaca aatgataaga
     3121 gtcctcatac ataaagactc cattagaaac gtcagtgacc caggagccca gaccaacagc
     3181 aaagcagaca gtgaaggatc cacagcctga ctaactcgac aaaccccccc aaacctaagg
     3241 tgagttgatc tttaagcttt ttacattttc agctcgcata tatcaattcg cgcgcattgt
     3301 cacactagca cccactagcg gtcagactgc agaTTGCAGC AcGaaacagg aagctgactc
     3361 cacatggtca catgctcact gaagtgttga cttccctgac agctgtgcac tttctaaacc
     3421 ggttttctca ttcatttaca gttcagccgg aattcacctc gagaccatgg ctccaaagaa
     3481 gaagcgtaag gtaaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     3541 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     3601 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     3661 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     3721 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     3781 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     3841 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     3901 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     3961 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     4021 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     4081 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     4141 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     4201 cgagctgtac aagtaaagat ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4261 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4321 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4381 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4441 tatggcttct gaggcggaaa gaaccaaagg gcgaattaat tcattgatga gtttggacaa
     4501 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     4561 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     4621 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     4681 tgtggtcatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     4741 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     4801 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     4861 ttaaagcaag taaaacctct acaaatgtgg tcattcagat caattaaccc tcacccgggc
     4921 tagcgatgca tgggagctct gacgtacgtt gtctagcaag gaagtgaaga gctatccgga
     4981 tatatatgta tgttatcata actgtactgg ctatgtcagg tggtaatgac tgtaacgtta
     5041 cgttactcgt tgtaggcacg acattgaata tgctcttctC CGCTGTGGCC CGAACGCCTC
     5101 CCgctagcga tgggatctaa tacgactcac tatagggaga ccaagggaaa atagaatgaa
     5161 gtgatctcca aaaaataagt actttttgac tgtaaataaa attgtaagga gtaaaaagta
     5221 cttttttttc taaaaaaatg taattaagta aaagtaaaag tattgatttt taattgtact
     5281 caagtaaagt aaaaatcccc aaaaataata cttaagtaca gtaatcaagt aaaattactc
     5341 aagtacttta cacctctggg atccagctgc atcatgaaca ataaaactgt ctgcttacat
     5401 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt
     5461 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     5521 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
     5581 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     5641 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     5701 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga
     5761 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     5821 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     5881 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     5941 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
     6001 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     6061 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     6121 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     6181 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     6241 ggttaattgg ttgtaacact ggcaaaaagg atctaggtga agatcctttt tgataatctc
     6301 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6361 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6421 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6481 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6541 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6601 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6661 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6721 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6781 acggttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6841 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgt
//
