LOCUS       pPRISM_Gal4_VP16_gcry_mRFP        6907 bp ds-DNA     circular     11-SEP-2018
DEFINITION  synthetic circular DNA, gene-targeting cassette
ACCESSION   .
VERSION     2.1
KEYWORDS    pPRISM, gal4-VP16
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Jordan M Welker,  Maira P Almeida, Melanie Torrie, Wesley A Wierson, Carla M Mann, Trevor Weiss, Cassandra Bullard, Katherine Knudtson, Kenna McKeighan, Stephen C. Ekker, Drena L Dobbs, Maura McGrail, Karl J Clark, Jeffrey J Essner
  TITLE     pPRISM an expanded tool set for precise genome editing
  JOURNAL   .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     misc_feature    complement(267..457)
                     /note="ITR(R)"
                     /note="Tol2 ITR(R)"
                     /locus_tag="ITR(R)"
                     /label="ITR(R)"
                     /ApEinfo_label="ITR(R)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="#80ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             994..1650
                     /locus_tag="*Gal4-VP16"
                     /label="*Gal4-VP16"
                     /ApEinfo_label="*Gal4-VP16"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             937..993
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /locus_tag="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label="2A peptide from porcine teschovirus-1 polyprotein"
                     /ApEinfo_label="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /ApEinfo_fwdcolor="#00ff80"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    918..923
                     /locus_tag="BfuAI site 2"
                     /label="BfuAI site 2"
                     /ApEinfo_label="BfuAI site 2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5158..5372
                     /note="ITR(L)"
                     /note="Tol2 ITR(L)"
                     /locus_tag="ITR(L)"
                     /label="ITR(L)"
                     /ApEinfo_label="ITR(L)"
                     /ApEinfo_fwdcolor="#80ffff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             5437..6246
                     /note="Kan(R)"
                     /locus_tag="Kan(R)"
                     /label="Kan(R)"
                     /ApEinfo_label="Kan(R)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    928..931
                     /locus_tag="BfuAI site 2 overhang"
                     /label="BfuAI site 2 overhang"
                     /ApEinfo_label="BfuAI site 2 overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(6279..6907)
                     /direction=LEFT
                     /note="ColE1 Ori"
                     /locus_tag="ColE1 Ori"
                     /label="ColE1 Ori"
                     /ApEinfo_label="ColE1 Ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(514..519)
                     /locus_tag="BfuAI site 1"
                     /label="BfuAI site 1"
                     /ApEinfo_label="BfuAI site 1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1..253)
                     /direction=LEFT
                     /note="ColE1 Ori"
                     /locus_tag="ColE1 Ori(1)"
                     /label="ColE1 Ori(1)"
                     /ApEinfo_label="ColE1 Ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(506..509)
                     /locus_tag="BfuAI site 1 overhang"
                     /label="BfuAI site 1 overhang"
                     /ApEinfo_label="BfuAI site 1 overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    487..509
                     /locus_tag="5' Universal Guide (w/PAM)"
                     /label="5' Universal Guide (w/PAM)"
                     /ApEinfo_label="5' Universal Guide (w/PAM)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    642..917
                     /vntifkey="21"
                     /locus_tag="lacZ'"
                     /label="lacZ'"
                     /ApEinfo_label="lacZ'"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="#1dda7b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        520..641
                     /vntifkey="30"
                     /locus_tag="lacZ\promoter"
                     /label="lacZ\promoter"
                     /ApEinfo_label="lacZ\promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5094..5116)
                     /locus_tag="3' Universal Guide (w/PAM)"
                     /label="3' Universal Guide (w/PAM)"
                     /ApEinfo_label="3' Universal Guide (w/PAM)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          4995..5083
                     /note="Carp beta-actin intron 1 filler"
                     /locus_tag="Carp beta-actin intron 1 filler"
                     /label="Carp beta-actin intron 1 filler"
                     /ApEinfo_label="Carp beta-actin intron 1 filler"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2878..3238
                     /note="gamma-crystallin"
                     /note="Xenopus"
                     /locus_tag="gamma-crystallin"
                     /label="gamma-crystallin"
                     /ApEinfo_label="gamma-crystallin"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5086..5092
                     /locus_tag="BspQI site 2"
                     /label="BspQI site 2"
                     /ApEinfo_label="BspQI site 2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    4496..4700
                     /note="SV40(A)"
                     /note="SV40"
                     /locus_tag="SV40(A)"
                     /label="SV40(A)"
                     /ApEinfo_label="SV40(A)"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4980..4986)
                     /locus_tag="BspQI site 1"
                     /label="BspQI site 1"
                     /ApEinfo_label="BspQI site 1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3548..4225
                     /vntifkey="4"
                     /note="mRFP with AUG removed"
                     /locus_tag="mRFP (RP2)"
                     /label="mRFP (RP2)"
                     /ApEinfo_label="mRFP (RP2)"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4976..4978)
                     /locus_tag="BspQI site 1 overhang"
                     /label="BspQI site 1 overhang"
                     /ApEinfo_label="BspQI site 1 overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    4701..4905
                     /note="SV40(A)"
                     /note="SV40"
                     /locus_tag="SV40(A)(1)"
                     /label="SV40(A)(1)"
                     /ApEinfo_label="SV40(A)"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5094..5096
                     /locus_tag="BspQI site 2 overhang"
                     /label="BspQI site 2 overhang"
                     /ApEinfo_label="BspQI site 2 overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          3291..3497
                     /note="mIntron"
                     /note="carp bactin shortened intron I"
                     /locus_tag="mIntron"
                     /label="mIntron"
                     /ApEinfo_label="mIntron"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    1659..2834
                     /note="ocean pout terminator"
                     /note="3'UTR, poly(A) signal, and putative boundary
                     element from ocean pout (FRM- Pat Gibbs)."
                     /locus_tag="ocean pout terminator"
                     /label="ocean pout terminator"
                     /ApEinfo_label="ocean pout terminator"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     3518..3547
                     /note="NLS"
                     /locus_tag="NLS"
                     /label="NLS"
                     /ApEinfo_label="NLS"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    4235..4459
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
                     /locus_tag="bGH poly(A) signal"
                     /label="bGH poly(A) signal"
                     /ApEinfo_label="bGH poly(A) signal"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
       61 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      121 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
      181 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      241 ggaagcggaa cagatttaaa tggtaccgag cagaggtgta aaaagtactc aaaaatttta
      301 ctcaagtgaa agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg
      361 gctagaatct tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt
      421 aaggaagtaa aagtaaaagc aagaaagaaa actagagtgg tctcccttta gtgagggtca
      481 attgatGGGA GGCGTTCGGG CCACAGCGGa cacgcaggtg cgcaacgcaa ttaatgtgag
      541 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      601 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      661 gcgcgcaatt aaccctcact aaagggaaca aaagctggag gacctcgagg gggggcccgg
      721 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
      781 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      841 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      901 gcctgaatgg cgaatggacc tgccacactt cccggagcca cgaacttctc tctgttaaag
      961 caagcaggag acgtggaaga aaaccccggt ccttCtAGaA AGCTACTGTC TTCTATCGAA
     1021 CAAGCATGCG ATATTTGCCG ACTTAAAAAG CTCAAGTGCT CCAAAGAAAA ACCGAAGTGC
     1081 GCCAAGTGTC TGAAGAACAA CTGGGAGTGT CGCTACTCTC CCAAAACCAA AAGGTCTCCG
     1141 CTGACTAGGG CACATCTGAC AGAAGTGGAA TCAAGGCTAG AAAGACTGGA ACAGCTATTT
     1201 CTACTGATTT TTCCTCGAGA AGACCTTGAC ATGATTTTGA AAATGGATTC TTTACAGGAT
     1261 ATAAAAGCAT TGTTAACAGG ATTATTTGTA CAAGATAATG TGAATAAAGA TGCCGTCACA
     1321 GATAGATTGG CTTCAGTGGA GACTGATATG CCTCTAACAT TGAGACAGCA TAGAATAAGT
     1381 GCGACATCAT CATCGGAAGA GAGTAGTAAC AAAGGTCAAA GACAGTTGAC TGTATCGTCG
     1441 AGGTCGACCC CGGGAATaCA GATCTCTCGA GCCGCCCCCC CGACCGATGT CAGCCTGGGG
     1501 GACGAGCTCC ACTTAGACGG CGAGGACGTG GCGATGGCGC ATGCCGACGC GCTAGACGAT
     1561 TTCGATCTGG ACATGTTGGG GGACGGGGAT TCCCCGGGTC CGGGATTTAC CCCCCACGAC
     1621 TCCGCCCCCT ACGGCGCTCT GGATATGTAA aactagtaat taagtctcag ccaccgttaa
     1681 ctgaacatgt caaaacctgt ggagactgtt gagatttgat gttctgaaaa gataaagcct
     1741 ataaataaaa tgttgcccaa atttcctgcc tgatgttttt ctttgtcttt gctacatggc
     1801 tttgctgctc ggatcggctc actctgtgta tgccacgttc actttgtact ctccttctca
     1861 cggtaggttt attattttta gatgtgcagt tagtttctgt gaaataacac accacacact
     1921 gatattgtct gtgcattgac ttggtgagtg cacattgttt ttgatcttga catatttata
     1981 tttgattgat caggtgaact gtgtgaatct aaagtgctcc atacagatgt tctgcattga
     2041 aaatattctc attttattag tggaagtgag tgtatgccac atccaatcaa tttcagcaaa
     2101 caccccagta tgatttaatg caaaaaaatg aaggtatcaa acacgcatta ctactttgca
     2161 gttaaatatt taacatttat tccaacacga aaaaaagcag taaataacac tttgacaaac
     2221 acgtcaggac atcttatttt tgtcaccctc acaggcaatt tagtataata tattatatat
     2281 atatatatat catataataa tattcagtat aatatatata tatatatcat attataatat
     2341 tcagtataat ataaaacaca aacacatata tgtataatat aatataacat ttttatttat
     2401 tgagatgcct ctatggaccg tgttataaga agtaaagatc aggagaagta aacatgaagt
     2461 gtaattatga atactgatgt taaattaagc tatgatgagt tttcactgtt aatttaccat
     2521 ctcaattaaa tgttgatgcc tccatgacca agttaagcag atgagactga gacaactgta
     2581 gaagacaaga tgttcacttt gctgaatata gctggcttga cagttatcta tgactctata
     2641 aatatatata tatttttttt tttataaaat gatttattta taactatata tccatttctc
     2701 agacaggtgc ttcatatccc tcactcccgt agctgtccat gctggatctg tccccgttgt
     2761 ttttaaaaag ctaaataagt tattaacatg actgcatcca gcgagccaaa cctgtctggt
     2821 gtacagctac cagagaagct tgagatccta gtcaccggcg ttcctagttc tttaaactgt
     2881 tacaaagtgt ttggaccagc aactgaattg cactgtaaat taccacatca gggtctccgg
     2941 ggtcaacgct gctaagcaat ggactggaaa atttgtgaca ttgagttttt ggaataactg
     3001 ggaatgctgg ataactcttt gggtaaagct atggaacaaa agtaccagca tgaactaaga
     3061 aatgactgtc acagcacaac atcaaacaca atgcaagaca aaggttctct gcttttgcct
     3121 tacttgacag aaaaagtaag ctctaggttc atcgcatgca gacagcagca atacatgaca
     3181 tatataaagg ctggctcttt tctcccttcc attgaactga aacttccact cagtcagact
     3241 tgcgggggat ccacagcctg actaactcga caaacccccc caaacctaag gtgagttgat
     3301 ctttaagctt tttacatttt cagctcgcat atatcaattc gcgcgcattg tcacactagc
     3361 acccactagc ggtcagactg cagaTTGCAG CAcGaaacag gaagctgact ccacatggtc
     3421 acatgctcac tgaagtgttg acttccctga cagctgtgca ctttctaaac cggttttctc
     3481 attcatttac agttcagccg gaattcacct cgagaccatg gctccaaaga agaagcgtaa
     3541 ggtaaccatg gcctcctccg aggacgtcat caaggagttc atgcgcttca aggtgcgcat
     3601 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     3661 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
     3721 ggacatcctg tcccctcagt tccagtacgg ctccaaggcc tacgtgaagc accccgccga
     3781 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     3841 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     3901 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     3961 gaagaccatg ggctgggagg cctccaccga gcggatgtac cccgaggacg gcgccctgaa
     4021 gggcgagatc aagatgaggc tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa
     4081 gaccacctac atggccaaga agcccgtgca gctgcccggc gcctacaaga ccgacatcaa
     4141 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgcgccga
     4201 gggccgccac tccaccggcg cctaaagctg agatctgtgc cttctagttg ccagccatct
     4261 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     4321 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     4381 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     4441 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca aagggcgaat taattcattg
     4501 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     4561 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     4621 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     4681 aaaacctcta caaatgtggt cattgatgag tttggacaaa ccacaactag aatgcagtga
     4741 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     4801 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     4861 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtcattc agatcaatta
     4921 accctcaccc gggctagcga tgcatgggag ctctgacgta cgttgtctag caaggaagtg
     4981 aagagctatc cggatatata tgtatgttat cataactgta ctggctatgt caggtggtaa
     5041 tgactgtaac gttacgttac tcgttgtagg cacgacattg aatatgctct tctCCGCTGT
     5101 GGCCCGAACG CCTCCCgcta gcgatgggat ctaatacgac tcactatagg gagaccaagg
     5161 gaaaatagaa tgaagtgatc tccaaaaaat aagtactttt tgactgtaaa taaaattgta
     5221 aggagtaaaa agtacttttt tttctaaaaa aatgtaatta agtaaaagta aaagtattga
     5281 tttttaattg tactcaagta aagtaaaaat ccccaaaaat aatacttaag tacagtaatc
     5341 aagtaaaatt actcaagtac tttacacctc tgggatccag ctgcatcatg aacaataaaa
     5401 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     5461 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     5521 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     5581 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     5641 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     5701 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     5761 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     5821 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     5881 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     5941 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     6001 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     6061 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     6121 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     6181 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     6241 ttctaatcag aattggttaa ttggttgtaa cactggcaaa aaggatctag gtgaagatcc
     6301 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6361 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6421 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6481 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6541 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6601 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6661 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6721 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6781 tatgagaaag cgccacggtt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6841 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6901 gtcctgt     
//
