LOCUS pPRISM_Gal4_VP16_cmlc2_tagBFP 6820 bp ds-DNA circular 11-SEP-2018 DEFINITION synthetic circular DNA, gene-targeting cassette ACCESSION . VERSION 2.1 KEYWORDS pPRISM, gal4-VP16 SOURCE synthetic DNA construct ORGANISM recombinant plasmid REFERENCE 1 (bases 1 to 6820) AUTHORS Jordan M Welker, Maira P Almeida, Melanie Torrie, Wesley A Wierson, Carla M Mann, Trevor Weiss, Cassandra Bullard, Katherine Knudtson, Kenna McKeighan, Stephen C. Ekker, Drena L Dobbs, Maura McGrail, Karl J Clark, Jeffrey J Essner TITLE pPRISM an expanded tool set for precise genome editing JOURNAL . COMMENT COMMENT COMMENT ApEinfo:methylated:0 FEATURES Location/Qualifiers misc_feature complement(267..457) /note="ITR(R)" /note="Tol2 ITR(R)" /locus_tag="ITR(R)" /label="ITR(R)" /ApEinfo_label="ITR(R)" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="#80ffff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 994..1650 /locus_tag="*Gal4-VP16" /label="*Gal4-VP16" /ApEinfo_label="*Gal4-VP16" /ApEinfo_fwdcolor="#ff00ff" /ApEinfo_revcolor="#ff00ff" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 937..993 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /note="P2A" /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" /locus_tag="2A peptide from porcine teschovirus-1 polyprotein" /label="2A peptide from porcine teschovirus-1 polyprotein" /ApEinfo_label="2A peptide from porcine teschovirus-1 polyprotein" /ApEinfo_fwdcolor="#00ff80" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 918..923 /locus_tag="BfuAI site 2" /label="BfuAI site 2" /ApEinfo_label="BfuAI site 2" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5071..5285 /note="ITR(L)" /note="Tol2 ITR(L)" /locus_tag="ITR(L)" /label="ITR(L)" /ApEinfo_label="ITR(L)" /ApEinfo_fwdcolor="#80ffff" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 5350..6159 /note="Kan(R)" /locus_tag="Kan(R)" /label="Kan(R)" /ApEinfo_label="Kan(R)" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" intron 3180..3386 /note="mIntron" /note="carp bactin shortened intron I" /locus_tag="mIntron" /label="mIntron" /ApEinfo_label="mIntron" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 928..931 /locus_tag="BfuAI site 2 overhang" /label="BfuAI site 2 overhang" /ApEinfo_label="BfuAI site 2 overhang" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin complement(6192..6820) /direction=LEFT /note="ColE1 Ori" /locus_tag="ColE1 Ori" /label="ColE1 Ori" /ApEinfo_label="ColE1 Ori" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(514..519) /locus_tag="BfuAI site 1" /label="BfuAI site 1" /ApEinfo_label="BfuAI site 1" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" rep_origin complement(1..253) /direction=LEFT /note="ColE1 Ori" /locus_tag="ColE1 Ori(1)" /label="ColE1 Ori(1)" /ApEinfo_label="ColE1 Ori" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(506..509) /locus_tag="BfuAI site 1 overhang" /label="BfuAI site 1 overhang" /ApEinfo_label="BfuAI site 1 overhang" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 487..509 /locus_tag="5' Universal Guide (w/PAM)" /label="5' Universal Guide (w/PAM)" /ApEinfo_label="5' Universal Guide (w/PAM)" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 642..917 /vntifkey="21" /locus_tag="lacZ'" /label="lacZ'" /ApEinfo_label="lacZ'" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="#1dda7b" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" promoter 520..641 /vntifkey="30" /locus_tag="lacZ\promoter" /label="lacZ\promoter" /ApEinfo_label="lacZ\promoter" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 2861..3135 /note="cmlc2 enhancer/promoter xu lab" /locus_tag="cmlc2 enhancer/promoter xu lab" /label="cmlc2 enhancer/promoter xu lab" /ApEinfo_label="cmlc2 enhancer/promoter xu lab" /ApEinfo_fwdcolor="#ff80ff" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(5007..5029) /locus_tag="3' Universal Guide (w/PAM)" /label="3' Universal Guide (w/PAM)" /ApEinfo_label="3' Universal Guide (w/PAM)" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_signal 3407..3436 /note="NLS" /locus_tag="NLS" /label="NLS" /ApEinfo_label="NLS" /ApEinfo_fwdcolor="#ffff80" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" intron 4908..4996 /note="Carp beta-actin intron 1 filler" /locus_tag="Carp beta-actin intron 1 filler" /label="Carp beta-actin intron 1 filler" /ApEinfo_label="Carp beta-actin intron 1 filler" /ApEinfo_fwdcolor="pink" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 4999..5005 /locus_tag="BspQI site 2" /label="BspQI site 2" /ApEinfo_label="BspQI site 2" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_signal 4148..4372 /note="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" /locus_tag="bGH poly(A) signal" /label="bGH poly(A) signal" /ApEinfo_label="bGH poly(A) signal" /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(4893..4899) /locus_tag="BspQI site 1" /label="BspQI site 1" /ApEinfo_label="BspQI site 1" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_signal 1659..2834 /note="ocean pout terminator" /note="3'UTR, poly(A) signal, and putative boundary element from ocean pout (FRM- Pat Gibbs)." /locus_tag="ocean pout terminator" /label="ocean pout terminator" /ApEinfo_label="ocean pout terminator" /ApEinfo_fwdcolor="#ff8000" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature complement(4889..4891) /locus_tag="BspQI site 1 overhang" /label="BspQI site 1 overhang" /ApEinfo_label="BspQI site 1 overhang" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" CDS 3437..4138 /codon_start=1 /product="monomeric blue fluorescent protein" /note="TagBFP" /note="mammalian codon-optimized" /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIK V VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT A TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMAL K LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARY C DLPSKLGHKLN" /locus_tag="TagBFP, monomeric blue fluorescent protein" /label="TagBFP, monomeric blue fluorescent protein" /ApEinfo_label="TagBFP, monomeric blue fluorescent protein" /ApEinfo_fwdcolor="#8080ff" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" misc_feature 5007..5009 /locus_tag="BspQI site 2 overhang" /label="BspQI site 2 overhang" /ApEinfo_label="BspQI site 2 overhang" /ApEinfo_fwdcolor="cyan" /ApEinfo_revcolor="green" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_signal 4614..4818 /note="SV40(A)" /note="SV40" /locus_tag="SV40(A)" /label="SV40(A)" /ApEinfo_label="SV40(A)" /ApEinfo_fwdcolor="#ff80c0" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" polyA_signal 4409..4613 /note="SV40(A)" /note="SV40" /locus_tag="SV40(A)(1)" /label="SV40(A)(1)" /ApEinfo_label="SV40(A)" /ApEinfo_fwdcolor="#ff80c0" /ApEinfo_revcolor="pink" /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0" ORIGIN 1 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 61 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 121 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 181 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 241 ggaagcggaa cagatttaaa tggtaccgag cagaggtgta aaaagtactc aaaaatttta 301 ctcaagtgaa agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg 361 gctagaatct tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt 421 aaggaagtaa aagtaaaagc aagaaagaaa actagagtgg tctcccttta gtgagggtca 481 attgatGGGA GGCGTTCGGG CCACAGCGGa cacgcaggtg cgcaacgcaa ttaatgtgag 541 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 601 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 661 gcgcgcaatt aaccctcact aaagggaaca aaagctggag gacctcgagg gggggcccgg 721 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac 781 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 841 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca 901 gcctgaatgg cgaatggacc tgccacactt cccggagcca cgaacttctc tctgttaaag 961 caagcaggag acgtggaaga aaaccccggt ccttCtAGaA AGCTACTGTC TTCTATCGAA 1021 CAAGCATGCG ATATTTGCCG ACTTAAAAAG CTCAAGTGCT CCAAAGAAAA ACCGAAGTGC 1081 GCCAAGTGTC TGAAGAACAA CTGGGAGTGT CGCTACTCTC CCAAAACCAA AAGGTCTCCG 1141 CTGACTAGGG CACATCTGAC AGAAGTGGAA TCAAGGCTAG AAAGACTGGA ACAGCTATTT 1201 CTACTGATTT TTCCTCGAGA AGACCTTGAC ATGATTTTGA AAATGGATTC TTTACAGGAT 1261 ATAAAAGCAT TGTTAACAGG ATTATTTGTA CAAGATAATG TGAATAAAGA TGCCGTCACA 1321 GATAGATTGG CTTCAGTGGA GACTGATATG CCTCTAACAT TGAGACAGCA TAGAATAAGT 1381 GCGACATCAT CATCGGAAGA GAGTAGTAAC AAAGGTCAAA GACAGTTGAC TGTATCGTCG 1441 AGGTCGACCC CGGGAATaCA GATCTCTCGA GCCGCCCCCC CGACCGATGT CAGCCTGGGG 1501 GACGAGCTCC ACTTAGACGG CGAGGACGTG GCGATGGCGC ATGCCGACGC GCTAGACGAT 1561 TTCGATCTGG ACATGTTGGG GGACGGGGAT TCCCCGGGTC CGGGATTTAC CCCCCACGAC 1621 TCCGCCCCCT ACGGCGCTCT GGATATGTAA aactagtaat taagtctcag ccaccgttaa 1681 ctgaacatgt caaaacctgt ggagactgtt gagatttgat gttctgaaaa gataaagcct 1741 ataaataaaa tgttgcccaa atttcctgcc tgatgttttt ctttgtcttt gctacatggc 1801 tttgctgctc ggatcggctc actctgtgta tgccacgttc actttgtact ctccttctca 1861 cggtaggttt attattttta gatgtgcagt tagtttctgt gaaataacac accacacact 1921 gatattgtct gtgcattgac ttggtgagtg cacattgttt ttgatcttga catatttata 1981 tttgattgat caggtgaact gtgtgaatct aaagtgctcc atacagatgt tctgcattga 2041 aaatattctc attttattag tggaagtgag tgtatgccac atccaatcaa tttcagcaaa 2101 caccccagta tgatttaatg caaaaaaatg aaggtatcaa acacgcatta ctactttgca 2161 gttaaatatt taacatttat tccaacacga aaaaaagcag taaataacac tttgacaaac 2221 acgtcaggac atcttatttt tgtcaccctc acaggcaatt tagtataata tattatatat 2281 atatatatat catataataa tattcagtat aatatatata tatatatcat attataatat 2341 tcagtataat ataaaacaca aacacatata tgtataatat aatataacat ttttatttat 2401 tgagatgcct ctatggaccg tgttataaga agtaaagatc aggagaagta aacatgaagt 2461 gtaattatga atactgatgt taaattaagc tatgatgagt tttcactgtt aatttaccat 2521 ctcaattaaa tgttgatgcc tccatgacca agttaagcag atgagactga gacaactgta 2581 gaagacaaga tgttcacttt gctgaatata gctggcttga cagttatcta tgactctata 2641 aatatatata tatttttttt tttataaaat gatttattta taactatata tccatttctc 2701 agacaggtgc ttcatatccc tcactcccgt agctgtccat gctggatctg tccccgttgt 2761 ttttaaaaag ctaaataagt tattaacatg actgcatcca gcgagccaaa cctgtctggt 2821 gtacagctac cagagaagct tgagatccta gtcaccggcg gcattcatcc atccttttca 2881 tccctcaaat ctctcattca cgtccccctc cccatctgca cactttatct cattttccac 2941 cctgctggaa tctgagcact tgtgcagtta tcagggctcc tgtatttagg aggctctggg 3001 tgtccatgta ggggacgaac agaaacactg cagaccttta tagaagaaca aatgataaga 3061 gtcctcatac ataaagactc cattagaaac gtcagtgacc caggagccca gaccaacagc 3121 aaagcagaca gtgaaggatc cacagcctga ctaactcgac aaaccccccc aaacctaagg 3181 tgagttgatc tttaagcttt ttacattttc agctcgcata tatcaattcg cgcgcattgt 3241 cacactagca cccactagcg gtcagactgc agaTTGCAGC AcGaaacagg aagctgactc 3301 cacatggtca catgctcact gaagtgttga cttccctgac agctgtgcac tttctaaacc 3361 ggttttctca ttcatttaca gttcagccgg aattcacctc gagaccatgg ctccaaagaa 3421 gaagcgtaag gtaaccatga gcgagctgat taaggagaac atgcacatga agctgtacat 3481 ggagggcacc gtggacaacc atcacttcaa gtgcacatcc gagggcgaag gcaagcccta 3541 cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt 3601 cgacatcctg gctactagct tcctctacgg cagcaagacc ttcatcaacc acacccaggg 3661 catccccgac ttcttcaagc agtccttccc tgagggcttc acatgggaga gagtcaccac 3721 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct 3781 catctacaac gtcaagatca gaggggtgaa cttcacatcc aacggccctg tgatgcagaa 3841 gaaaacactc ggctgggagg ccttcaccga gacgctgtac cccgctgacg gcggcctgga 3901 aggcagaaac gacatggccc tgaagctcgt gggcgggagc catctgatcg caaacatcaa 3961 gaccacatat agatccaaga aacccgctaa gaacctcaag atgcctggcg tctactatgt 4021 ggactacaga ctggaaagaa tcaaggaggc caacaacgag acctacgtcg agcagcacga 4081 ggtggcagtg gccagatact gcgacctccc tagcaaactg gggcacaagc tcaattaaag 4141 ctgagatctg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 4201 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 4261 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 4321 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag 4381 gcggaaagaa ccaaagggcg aattaattca ttgatgagtt tggacaaacc acaactagaa 4441 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca 4501 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc 4561 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtcattgat 4621 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt 4681 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat 4741 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa 4801 aacctctaca aatgtggtca ttcagatcaa ttaaccctca cccgggctag cgatgcatgg 4861 gagctctgac gtacgttgtc tagcaaggaa gtgaagagct atccggatat atatgtatgt 4921 tatcataact gtactggcta tgtcaggtgg taatgactgt aacgttacgt tactcgttgt 4981 aggcacgaca ttgaatatgc tcttctCCGC TGTGGCCCGA ACGCCTCCCg ctagcgatgg 5041 gatctaatac gactcactat agggagacca agggaaaata gaatgaagtg atctccaaaa 5101 aataagtact ttttgactgt aaataaaatt gtaaggagta aaaagtactt ttttttctaa 5161 aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa 5221 aatccccaaa aataatactt aagtacagta atcaagtaaa attactcaag tactttacac 5281 ctctgggatc cagctgcatc atgaacaata aaactgtctg cttacataaa cagtaataca 5341 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg 5401 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca 5461 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt 5521 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg 5581 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact 5641 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat 5701 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt 5761 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt 5821 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg 5881 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc 5941 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga 6001 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt 6061 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat 6121 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg 6181 taacactggc aaaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 6241 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 6301 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 6361 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 6421 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 6481 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 6541 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 6601 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 6661 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg gttcccgaag 6721 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 6781 agcttccagg gggaaacgcc tggtatcttt atagtcctgt //