LOCUS       pPRISM_Gal4_VP16_cmlc2_mRFP        6796 bp ds-DNA     circular     11-SEP-2018
DEFINITION  synthetic circular DNA, gene-targeting cassette
ACCESSION   .
VERSION     2.1
KEYWORDS    pPRISM, gal4-VP16
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Jordan M Welker,  Maira P Almeida, Melanie Torrie, Wesley A Wierson, Carla M Mann, Trevor Weiss, Cassandra Bullard, Katherine Knudtson, Kenna McKeighan, Stephen C. Ekker, Drena L Dobbs, Maura McGrail, Karl J Clark, Jeffrey J Essner
  TITLE     pPRISM an expanded tool set for precise genome editing
  JOURNAL   .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     misc_feature    complement(267..457)
                     /note="ITR(R)"
                     /note="Tol2 ITR(R)"
                     /locus_tag="ITR(R)"
                     /label="ITR(R)"
                     /ApEinfo_label="ITR(R)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="#80ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             994..1650
                     /locus_tag="*Gal4-VP16"
                     /label="*Gal4-VP16"
                     /ApEinfo_label="*Gal4-VP16"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             937..993
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /locus_tag="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label="2A peptide from porcine teschovirus-1 polyprotein"
                     /ApEinfo_label="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /ApEinfo_fwdcolor="#00ff80"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    918..923
                     /locus_tag="BfuAI site 2"
                     /label="BfuAI site 2"
                     /ApEinfo_label="BfuAI site 2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5047..5261
                     /note="ITR(L)"
                     /note="Tol2 ITR(L)"
                     /locus_tag="ITR(L)"
                     /label="ITR(L)"
                     /ApEinfo_label="ITR(L)"
                     /ApEinfo_fwdcolor="#80ffff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             5326..6135
                     /note="Kan(R)"
                     /locus_tag="Kan(R)"
                     /label="Kan(R)"
                     /ApEinfo_label="Kan(R)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    928..931
                     /locus_tag="BfuAI site 2 overhang"
                     /label="BfuAI site 2 overhang"
                     /ApEinfo_label="BfuAI site 2 overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3437..4114
                     /vntifkey="4"
                     /note="mRFP with AUG removed"
                     /locus_tag="mRFP (RP2)"
                     /label="mRFP (RP2)"
                     /ApEinfo_label="mRFP (RP2)"
                     /ApEinfo_fwdcolor="#ff0080"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(6168..6796)
                     /direction=LEFT
                     /note="ColE1 Ori"
                     /locus_tag="ColE1 Ori"
                     /label="ColE1 Ori"
                     /ApEinfo_label="ColE1 Ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(514..519)
                     /locus_tag="BfuAI site 1"
                     /label="BfuAI site 1"
                     /ApEinfo_label="BfuAI site 1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1..253)
                     /direction=LEFT
                     /note="ColE1 Ori"
                     /locus_tag="ColE1 Ori(1)"
                     /label="ColE1 Ori(1)"
                     /ApEinfo_label="ColE1 Ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(506..509)
                     /locus_tag="BfuAI site 1 overhang"
                     /label="BfuAI site 1 overhang"
                     /ApEinfo_label="BfuAI site 1 overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    487..509
                     /locus_tag="5' Universal Guide (w/PAM)"
                     /label="5' Universal Guide (w/PAM)"
                     /ApEinfo_label="5' Universal Guide (w/PAM)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    642..917
                     /vntifkey="21"
                     /locus_tag="lacZ'"
                     /label="lacZ'"
                     /ApEinfo_label="lacZ'"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="#1dda7b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          3180..3386
                     /note="mIntron"
                     /note="carp bactin shortened intron I"
                     /locus_tag="mIntron"
                     /label="mIntron"
                     /ApEinfo_label="mIntron"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        520..641
                     /vntifkey="30"
                     /locus_tag="lacZ\promoter"
                     /label="lacZ\promoter"
                     /ApEinfo_label="lacZ\promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4983..5005)
                     /locus_tag="3' Universal Guide (w/PAM)"
                     /label="3' Universal Guide (w/PAM)"
                     /ApEinfo_label="3' Universal Guide (w/PAM)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          4884..4972
                     /note="Carp beta-actin intron 1 filler"
                     /locus_tag="Carp beta-actin intron 1 filler"
                     /label="Carp beta-actin intron 1 filler"
                     /ApEinfo_label="Carp beta-actin intron 1 filler"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4975..4981
                     /locus_tag="BspQI site 2"
                     /label="BspQI site 2"
                     /ApEinfo_label="BspQI site 2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4869..4875)
                     /locus_tag="BspQI site 1"
                     /label="BspQI site 1"
                     /ApEinfo_label="BspQI site 1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     3407..3436
                     /note="NLS"
                     /locus_tag="NLS"
                     /label="NLS"
                     /ApEinfo_label="NLS"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4865..4867)
                     /locus_tag="BspQI site 1 overhang"
                     /label="BspQI site 1 overhang"
                     /ApEinfo_label="BspQI site 1 overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    4385..4589
                     /note="SV40(A)"
                     /note="SV40"
                     /locus_tag="SV40(A)"
                     /label="SV40(A)"
                     /ApEinfo_label="SV40(A)"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4983..4985
                     /locus_tag="BspQI site 2 overhang"
                     /label="BspQI site 2 overhang"
                     /ApEinfo_label="BspQI site 2 overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2861..3135
                     /note="cmlc2 enhancer/promoter xu lab"
                     /locus_tag="cmlc2 enhancer/promoter xu lab"
                     /label="cmlc2 enhancer/promoter xu lab"
                     /ApEinfo_label="cmlc2 enhancer/promoter xu lab"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    1659..2834
                     /note="ocean pout terminator"
                     /note="3'UTR, poly(A) signal, and putative boundary
                     element from ocean pout (FRM- Pat Gibbs)."
                     /locus_tag="ocean pout terminator"
                     /label="ocean pout terminator"
                     /ApEinfo_label="ocean pout terminator"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    4124..4348
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
                     /locus_tag="bGH poly(A) signal"
                     /label="bGH poly(A) signal"
                     /ApEinfo_label="bGH poly(A) signal"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    4590..4794
                     /note="SV40(A)"
                     /note="SV40"
                     /locus_tag="SV40(A)(1)"
                     /label="SV40(A)(1)"
                     /ApEinfo_label="SV40(A)"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
       61 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      121 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
      181 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      241 ggaagcggaa cagatttaaa tggtaccgag cagaggtgta aaaagtactc aaaaatttta
      301 ctcaagtgaa agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg
      361 gctagaatct tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt
      421 aaggaagtaa aagtaaaagc aagaaagaaa actagagtgg tctcccttta gtgagggtca
      481 attgatGGGA GGCGTTCGGG CCACAGCGGa cacgcaggtg cgcaacgcaa ttaatgtgag
      541 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      601 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      661 gcgcgcaatt aaccctcact aaagggaaca aaagctggag gacctcgagg gggggcccgg
      721 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
      781 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      841 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      901 gcctgaatgg cgaatggacc tgccacactt cccggagcca cgaacttctc tctgttaaag
      961 caagcaggag acgtggaaga aaaccccggt ccttCtAGaA AGCTACTGTC TTCTATCGAA
     1021 CAAGCATGCG ATATTTGCCG ACTTAAAAAG CTCAAGTGCT CCAAAGAAAA ACCGAAGTGC
     1081 GCCAAGTGTC TGAAGAACAA CTGGGAGTGT CGCTACTCTC CCAAAACCAA AAGGTCTCCG
     1141 CTGACTAGGG CACATCTGAC AGAAGTGGAA TCAAGGCTAG AAAGACTGGA ACAGCTATTT
     1201 CTACTGATTT TTCCTCGAGA AGACCTTGAC ATGATTTTGA AAATGGATTC TTTACAGGAT
     1261 ATAAAAGCAT TGTTAACAGG ATTATTTGTA CAAGATAATG TGAATAAAGA TGCCGTCACA
     1321 GATAGATTGG CTTCAGTGGA GACTGATATG CCTCTAACAT TGAGACAGCA TAGAATAAGT
     1381 GCGACATCAT CATCGGAAGA GAGTAGTAAC AAAGGTCAAA GACAGTTGAC TGTATCGTCG
     1441 AGGTCGACCC CGGGAATaCA GATCTCTCGA GCCGCCCCCC CGACCGATGT CAGCCTGGGG
     1501 GACGAGCTCC ACTTAGACGG CGAGGACGTG GCGATGGCGC ATGCCGACGC GCTAGACGAT
     1561 TTCGATCTGG ACATGTTGGG GGACGGGGAT TCCCCGGGTC CGGGATTTAC CCCCCACGAC
     1621 TCCGCCCCCT ACGGCGCTCT GGATATGTAA aactagtaat taagtctcag ccaccgttaa
     1681 ctgaacatgt caaaacctgt ggagactgtt gagatttgat gttctgaaaa gataaagcct
     1741 ataaataaaa tgttgcccaa atttcctgcc tgatgttttt ctttgtcttt gctacatggc
     1801 tttgctgctc ggatcggctc actctgtgta tgccacgttc actttgtact ctccttctca
     1861 cggtaggttt attattttta gatgtgcagt tagtttctgt gaaataacac accacacact
     1921 gatattgtct gtgcattgac ttggtgagtg cacattgttt ttgatcttga catatttata
     1981 tttgattgat caggtgaact gtgtgaatct aaagtgctcc atacagatgt tctgcattga
     2041 aaatattctc attttattag tggaagtgag tgtatgccac atccaatcaa tttcagcaaa
     2101 caccccagta tgatttaatg caaaaaaatg aaggtatcaa acacgcatta ctactttgca
     2161 gttaaatatt taacatttat tccaacacga aaaaaagcag taaataacac tttgacaaac
     2221 acgtcaggac atcttatttt tgtcaccctc acaggcaatt tagtataata tattatatat
     2281 atatatatat catataataa tattcagtat aatatatata tatatatcat attataatat
     2341 tcagtataat ataaaacaca aacacatata tgtataatat aatataacat ttttatttat
     2401 tgagatgcct ctatggaccg tgttataaga agtaaagatc aggagaagta aacatgaagt
     2461 gtaattatga atactgatgt taaattaagc tatgatgagt tttcactgtt aatttaccat
     2521 ctcaattaaa tgttgatgcc tccatgacca agttaagcag atgagactga gacaactgta
     2581 gaagacaaga tgttcacttt gctgaatata gctggcttga cagttatcta tgactctata
     2641 aatatatata tatttttttt tttataaaat gatttattta taactatata tccatttctc
     2701 agacaggtgc ttcatatccc tcactcccgt agctgtccat gctggatctg tccccgttgt
     2761 ttttaaaaag ctaaataagt tattaacatg actgcatcca gcgagccaaa cctgtctggt
     2821 gtacagctac cagagaagct tgagatccta gtcaccggcg gcattcatcc atccttttca
     2881 tccctcaaat ctctcattca cgtccccctc cccatctgca cactttatct cattttccac
     2941 cctgctggaa tctgagcact tgtgcagtta tcagggctcc tgtatttagg aggctctggg
     3001 tgtccatgta ggggacgaac agaaacactg cagaccttta tagaagaaca aatgataaga
     3061 gtcctcatac ataaagactc cattagaaac gtcagtgacc caggagccca gaccaacagc
     3121 aaagcagaca gtgaaggatc cacagcctga ctaactcgac aaaccccccc aaacctaagg
     3181 tgagttgatc tttaagcttt ttacattttc agctcgcata tatcaattcg cgcgcattgt
     3241 cacactagca cccactagcg gtcagactgc agaTTGCAGC AcGaaacagg aagctgactc
     3301 cacatggtca catgctcact gaagtgttga cttccctgac agctgtgcac tttctaaacc
     3361 ggttttctca ttcatttaca gttcagccgg aattcacctc gagaccatgg ctccaaagaa
     3421 gaagcgtaag gtaaccatgg cctcctccga ggacgtcatc aaggagttca tgcgcttcaa
     3481 ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     3541 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
     3601 cttcgcctgg gacatcctgt cccctcagtt ccagtacggc tccaaggcct acgtgaagca
     3661 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     3721 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     3781 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     3841 aatgcagaag aagaccatgg gctgggaggc ctccaccgag cggatgtacc ccgaggacgg
     3901 cgccctgaag ggcgagatca agatgaggct gaagctgaag gacggcggcc actacgacgc
     3961 cgaggtcaag accacctaca tggccaagaa gcccgtgcag ctgcccggcg cctacaagac
     4021 cgacatcaag ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     4081 gcgcgccgag ggccgccact ccaccggcgc ctaaagctga gatctgtgcc ttctagttgc
     4141 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     4201 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     4261 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     4321 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccaa agggcgaatt
     4381 aattcattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     4441 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     4501 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     4561 aaagcaagta aaacctctac aaatgtggtc attgatgagt ttggacaaac cacaactaga
     4621 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     4681 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     4741 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtcattca
     4801 gatcaattaa ccctcacccg ggctagcgat gcatgggagc tctgacgtac gttgtctagc
     4861 aaggaagtga agagctatcc ggatatatat gtatgttatc ataactgtac tggctatgtc
     4921 aggtggtaat gactgtaacg ttacgttact cgttgtaggc acgacattga atatgctctt
     4981 ctCCGCTGTG GCCCGAACGC CTCCCgctag cgatgggatc taatacgact cactataggg
     5041 agaccaaggg aaaatagaat gaagtgatct ccaaaaaata agtacttttt gactgtaaat
     5101 aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa gtaaaagtaa
     5161 aagtattgat ttttaattgt actcaagtaa agtaaaaatc cccaaaaata atacttaagt
     5221 acagtaatca agtaaaatta ctcaagtact ttacacctct gggatccagc tgcatcatga
     5281 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     5341 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     5401 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     5461 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     5521 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     5581 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     5641 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     5701 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     5761 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     5821 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     5881 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     5941 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     6001 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     6061 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     6121 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcaaaa aggatctagg
     6181 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6241 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6301 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6361 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6421 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6481 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6541 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6601 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6661 agcgtgagct atgagaaagc gccacggttc ccgaagggag aaaggcggac aggtatccgg
     6721 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6781 atctttatag tcctgt    
//
