LOCUS       pPRISM_Fuse_cmlc2_eGFP        6815 bp ds-DNA     circular     11-SEP-2018
DEFINITION  synthetic circular DNA, gene-targeting cassette
ACCESSION   .
VERSION     2.1
KEYWORDS    pPRISM, 3'-fusion
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Jordan M Welker,  Maira P Almeida, Melanie Torrie, Wesley A Wierson, Carla M Mann, Trevor Weiss, Cassandra Bullard, Katherine Knudtson, Kenna McKeighan, Stephen C. Ekker, Drena L Dobbs, Maura McGrail, Karl J Clark, Jeffrey J Essner
  TITLE     pPRISM an expanded tool set for precise genome editing
  JOURNAL   .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:0
FEATURES             Location/Qualifiers
     misc_feature    931..974
                     /locus_tag="Flexible Linker"
                     /label="Flexible Linker"
                     /ApEinfo_label="Flexible Linker"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    487..509
                     /locus_tag="5' Universal Guide (w/PAM)"
                     /label="5' Universal Guide (w/PAM)"
                     /ApEinfo_label="5' Universal Guide (w/PAM)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5002..5024)
                     /locus_tag="3' Universal Guide (w/PAM)"
                     /label="3' Universal Guide (w/PAM)"
                     /ApEinfo_label="3' Universal Guide (w/PAM)"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(267..457)
                     /note="ITR(R)"
                     /note="Tol2 ITR(R)"
                     /locus_tag="ITR(R)"
                     /label="ITR(R)"
                     /ApEinfo_label="ITR(R)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="#80ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          4903..4991
                     /note="Carp beta-actin intron 1 filler"
                     /locus_tag="Carp beta-actin intron 1 filler"
                     /label="Carp beta-actin intron 1 filler"
                     /ApEinfo_label="Carp beta-actin intron 1 filler"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5066..5280
                     /note="ITR(L)"
                     /note="Tol2 ITR(L)"
                     /locus_tag="ITR(L)"
                     /label="ITR(L)"
                     /ApEinfo_label="ITR(L)"
                     /ApEinfo_fwdcolor="#80ffff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             5345..6154
                     /note="Kan(R)"
                     /locus_tag="Kan(R)"
                     /label="Kan(R)"
                     /ApEinfo_label="Kan(R)"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(6187..6815)
                     /direction=LEFT
                     /note="ColE1 Ori"
                     /locus_tag="ColE1 Ori"
                     /label="ColE1 Ori"
                     /ApEinfo_label="ColE1 Ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(1..253)
                     /direction=LEFT
                     /note="ColE1 Ori"
                     /locus_tag="ColE1 Ori(1)"
                     /label="ColE1 Ori(1)"
                     /ApEinfo_label="ColE1 Ori"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          3162..3368
                     /note="mIntron"
                     /note="carp bactin shortened intron I"
                     /locus_tag="mIntron"
                     /label="mIntron"
                     /ApEinfo_label="mIntron"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4994..5000
                     /locus_tag="BspQI site 2"
                     /label="BspQI site 2"
                     /ApEinfo_label="BspQI site 2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4888..4894)
                     /locus_tag="BspQI site 1"
                     /label="BspQI site 1"
                     /ApEinfo_label="BspQI site 1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(4884..4886)
                     /locus_tag="BspQI site 1 overhang"
                     /label="BspQI site 1 overhang"
                     /ApEinfo_label="BspQI site 1 overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5002..5004
                     /locus_tag="BspQI site 2 overhang"
                     /label="BspQI site 2 overhang"
                     /ApEinfo_label="BspQI site 2 overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(506..509)
                     /locus_tag="BfuAI site 1 overhang"
                     /label="BfuAI site 1 overhang"
                     /ApEinfo_label="BfuAI site 1 overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    918..923
                     /locus_tag="BfuAI site 2"
                     /label="BfuAI site 2"
                     /ApEinfo_label="BfuAI site 2"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    1641..2816
                     /note="ocean pout terminator"
                     /note="3'UTR, poly(A) signal, and putative boundary
                     element from ocean pout (FRM- Pat Gibbs)."
                     /locus_tag="ocean pout terminator"
                     /label="ocean pout terminator"
                     /ApEinfo_label="ocean pout terminator"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(514..519)
                     /locus_tag="BfuAI site 1"
                     /label="BfuAI site 1"
                     /ApEinfo_label="BfuAI site 1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    4143..4367
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
                     /locus_tag="bGH poly(A) signal"
                     /label="bGH poly(A) signal"
                     /ApEinfo_label="bGH poly(A) signal"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        520..641
                     /vntifkey="30"
                     /locus_tag="lacZ\promoter"
                     /label="lacZ\promoter"
                     /ApEinfo_label="lacZ\promoter"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    4404..4608
                     /note="SV40(A)"
                     /note="SV40"
                     /locus_tag="SV40(A)"
                     /label="SV40(A)"
                     /ApEinfo_label="SV40(A)"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    642..917
                     /vntifkey="21"
                     /locus_tag="lacZ'"
                     /label="lacZ'"
                     /ApEinfo_label="lacZ'"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="#1dda7b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2843..3117
                     /note="cmlc2 enhancer/promoter xu lab"
                     /locus_tag="cmlc2 enhancer/promoter xu lab"
                     /label="cmlc2 enhancer/promoter xu lab"
                     /ApEinfo_label="cmlc2 enhancer/promoter xu lab"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             975..1632
                     /note="mRFP*"
                     /locus_tag="mRFP*"
                     /label="mRFP*"
                     /ApEinfo_label="mRFP*"
                     /ApEinfo_fwdcolor="#fc45e0"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_signal     3389..3418
                     /note="NLS"
                     /locus_tag="NLS"
                     /label="NLS"
                     /ApEinfo_label="NLS"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    928..931
                     /locus_tag="BfuAI site 2 overhang"
                     /label="BfuAI site 2 overhang"
                     /ApEinfo_label="BfuAI site 2 overhang"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3419..4138
                     /vntifkey="4"
                     /locus_tag="eGFP"
                     /label="eGFP"
                     /ApEinfo_label="eGFP"
                     /ApEinfo_fwdcolor="#00ff0e"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    4609..4813
                     /note="SV40(A)"
                     /note="SV40"
                     /locus_tag="SV40(A)(1)"
                     /label="SV40(A)(1)"
                     /ApEinfo_label="SV40(A)"
                     /ApEinfo_fwdcolor="#ff80c0"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
       61 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      121 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
      181 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      241 ggaagcggaa cagatttaaa tggtaccgag cagaggtgta aaaagtactc aaaaatttta
      301 ctcaagtgaa agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg
      361 gctagaatct tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt
      421 aaggaagtaa aagtaaaagc aagaaagaaa actagagtgg tctcccttta gtgagggtca
      481 attgatGGGA GGCGTTCGGG CCACAGCGGa cacgcaggtg cgcaacgcaa ttaatgtgag
      541 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      601 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      661 gcgcgcaatt aaccctcact aaagggaaca aaagctggag gacctcgagg gggggcccgg
      721 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
      781 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      841 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      901 gcctgaatgg cgaatggacc tgccacactt ctaggaggag gtagcggagg tagcggagcc
      961 tcctccgagg acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg
     1021 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     1081 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     1141 cctcagttcc agtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     1201 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     1261 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     1321 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     1381 tgggaggcct ccaccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     1441 atgaggctga agctgaagga cggcggccac tacgacgccg aggtcaagac cacctacatg
     1501 gccaagaagc ccgtgcagct gcccggcgcc tacaagaccg acatcaagct ggacatcacc
     1561 tcccacaacg aggactacac catcgtggaa cagtacgagc gcgccgaggg ccgccactcc
     1621 accggagcct aagactagta attaagtctc agccaccgtt aactgaacat gtcaaaacct
     1681 gtggagactg ttgagatttg atgttctgaa aagataaagc ctataaataa aatgttgccc
     1741 aaatttcctg cctgatgttt ttctttgtct ttgctacatg gctttgctgc tcggatcggc
     1801 tcactctgtg tatgccacgt tcactttgta ctctccttct cacggtaggt ttattatttt
     1861 tagatgtgca gttagtttct gtgaaataac acaccacaca ctgatattgt ctgtgcattg
     1921 acttggtgag tgcacattgt ttttgatctt gacatattta tatttgattg atcaggtgaa
     1981 ctgtgtgaat ctaaagtgct ccatacagat gttctgcatt gaaaatattc tcattttatt
     2041 agtggaagtg agtgtatgcc acatccaatc aatttcagca aacaccccag tatgatttaa
     2101 tgcaaaaaaa tgaaggtatc aaacacgcat tactactttg cagttaaata tttaacattt
     2161 attccaacac gaaaaaaagc agtaaataac actttgacaa acacgtcagg acatcttatt
     2221 tttgtcaccc tcacaggcaa tttagtataa tatattatat atatatatat atcatataat
     2281 aatattcagt ataatatata tatatatatc atattataat attcagtata atataaaaca
     2341 caaacacata tatgtataat ataatataac atttttattt attgagatgc ctctatggac
     2401 cgtgttataa gaagtaaaga tcaggagaag taaacatgaa gtgtaattat gaatactgat
     2461 gttaaattaa gctatgatga gttttcactg ttaatttacc atctcaatta aatgttgatg
     2521 cctccatgac caagttaagc agatgagact gagacaactg tagaagacaa gatgttcact
     2581 ttgctgaata tagctggctt gacagttatc tatgactcta taaatatata tatatttttt
     2641 tttttataaa atgatttatt tataactata tatccatttc tcagacaggt gcttcatatc
     2701 cctcactccc gtagctgtcc atgctggatc tgtccccgtt gtttttaaaa agctaaataa
     2761 gttattaaca tgactgcatc cagcgagcca aacctgtctg gtgtacagct accagagaag
     2821 cttgagatcc tagtcaccgg cggcattcat ccatcctttt catccctcaa atctctcatt
     2881 cacgtccccc tccccatctg cacactttat ctcattttcc accctgctgg aatctgagca
     2941 cttgtgcagt tatcagggct cctgtattta ggaggctctg ggtgtccatg taggggacga
     3001 acagaaacac tgcagacctt tatagaagaa caaatgataa gagtcctcat acataaagac
     3061 tccattagaa acgtcagtga cccaggagcc cagaccaaca gcaaagcaga cagtgaagga
     3121 tccacagcct gactaactcg acaaaccccc ccaaacctaa ggtgagttga tctttaagct
     3181 ttttacattt tcagctcgca tatatcaatt cgcgcgcatt gtcacactag cacccactag
     3241 cggtcagact gcagaTTGCA GCAcGaaaca ggaagctgac tccacatggt cacatgctca
     3301 ctgaagtgtt gacttccctg acagctgtgc actttctaaa ccggttttct cattcattta
     3361 cagttcagcc ggaattcacc tcgagaccat ggctccaaag aagaagcgta aggtaaccat
     3421 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     3481 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     3541 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     3601 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     3661 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     3721 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     3781 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     3841 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     3901 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     3961 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     4021 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     4081 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag
     4141 atctgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     4201 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     4261 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     4321 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     4381 aagaaccaaa gggcgaatta attcattgat gagtttggac aaaccacaac tagaatgcag
     4441 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     4501 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     4561 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtca ttgatgagtt
     4621 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     4681 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     4741 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     4801 ctacaaatgt ggtcattcag atcaattaac cctcacccgg gctagcgatg catgggagct
     4861 ctgacgtacg ttgtctagca aggaagtgaa gagctatccg gatatatatg tatgttatca
     4921 taactgtact ggctatgtca ggtggtaatg actgtaacgt tacgttactc gttgtaggca
     4981 cgacattgaa tatgctcttc tCCGCTGTGG CCCGAACGCC TCCCgctagc gatgggatct
     5041 aatacgactc actataggga gaccaaggga aaatagaatg aagtgatctc caaaaaataa
     5101 gtactttttg actgtaaata aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa
     5161 tgtaattaag taaaagtaaa agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc
     5221 ccaaaaataa tacttaagta cagtaatcaa gtaaaattac tcaagtactt tacacctctg
     5281 ggatccagct gcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     5341 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
     5401 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     5461 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     5521 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     5581 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     5641 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     5701 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     5761 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     5821 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     5881 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     5941 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     6001 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     6061 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     6121 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
     6181 ctggcaaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6241 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6301 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6361 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6421 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6481 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6541 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6601 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6661 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacggttcc cgaagggaga
     6721 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6781 ccagggggaa acgcctggta tctttatagt cctgt  
//
