LOCUS       pBAD/fluoB              4997 bp    DNA     circular UNA 20-SEP-2018
DEFINITION  Control plasmid.
ACCESSION   urn.local...6c-9j28bqv
VERSION     urn.local...6c-9j28bqv
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    complement(357..1235)
                     /standard_name="AraC"
     misc_feature    1265..1280
                     /standard_name="araO2"
     misc_feature    1422..1433
                     /standard_name="araO1"
     misc_feature    1464..1477
                     /standard_name="CAP_BS"
     misc_feature    1469..1488
                     /standard_name="pBAD_fwd_primer"
     misc_feature    1474..1512
                     /standard_name="AraI1I2"
     promoter        1509..1537
                     /standard_name="ARA_promoter"
     misc_feature    1580..2620
                     /note="Geneious type: ORF"
                     /standard_name="CURT_fluoB"
     misc_feature    <1904..2617
                     /standard_name="mCitrine"
     terminator      2709..2866
                     /standard_name="rrnB_terminator"
     terminator      2832..2875
                     /standard_name="rrnB_T1_terminator"
     terminator      3007..3034
                     /standard_name="rrnB_T2_terminator"
     promoter        3075..3103
                     /standard_name="AmpR_promoter"
     gene            3145..4005
                     /gene="Ampicillin"
                     /note="Geneious type: misc. marker"
                     /standard_name="Ampicillin resistance (bla)"
     rep_origin      4160..4779
ORIGIN      
        1 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
       61 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
      121 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
      181 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcagatc
      241 aattcgcgcg cgaaggcgaa gcggcatgca taatgtgcct gtcaaatgga cgaagcaggg
      301 attctgcaaa ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt taccaattat
      361 gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg
      421 ctggccccgg tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca
      481 ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg
      541 atacgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt
      601 gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg
      661 tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga
      721 tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc
      781 gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg
      841 tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt
      901 gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga
      961 taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc
     1021 aaaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg
     1081 cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg
     1141 agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat
     1201 cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga
     1261 gaagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc
     1321 ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc
     1381 aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt
     1441 gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg
     1501 gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc gttttttggg
     1561 ctaacaggag gaattaacca tggcttcttc agaagagacc tcgtccattg ataccaacga
     1621 actcatcaca gatttgaagg aaaagtggga tggtcttgag aacaaatcta ctgtacttat
     1681 atatggagga ggagccattg ttgctgtttg gttatcttcc attgttgttg gtgccatcaa
     1741 ctctgttcct ctgcttccga aagttatgga acttgtcgga ctagggtaca ctggatggtt
     1801 tgtctacaga taccttctct tcaagtcaag cagaaaggaa ttggctgagg atattgaatc
     1861 cttgaagaag aagatcgcag gaagcgaagg aggtggtgga tctgtgagca agggcgagga
     1921 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1981 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2041 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta
     2101 cggcctgatg tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2161 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2221 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2281 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2341 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2401 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2461 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccaa
     2521 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2581 cgccgggatc actctcggca tggacgagct gtacaagtaa aagcttggct gttttggcgg
     2641 atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa
     2701 acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga
     2761 agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg
     2821 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     2881 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     2941 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
     3001 ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta
     3061 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     3121 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     3181 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     3241 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     3301 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     3361 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     3421 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     3481 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     3541 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     3601 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     3661 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     3721 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     3781 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     3841 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     3901 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     3961 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4021 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4081 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4141 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4201 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4261 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4321 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4381 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4441 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4501 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4561 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4621 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4681 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4741 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4801 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4861 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     4921 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     4981 tgcggtattt cacaccg
//
