LOCUS       pZK538-dspAVR2-G       13692 bp    DNA     circular     20-SEP-2018
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     terminator      111..363
                     /note="MAS terminator"
                     /note="mannopine synthase terminator"
                     /note="color: #ffffff"
     CDS             complement(373..924)
                     /dnas_title="phosphinothricin acetyltransferase"
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /function=""
                     /note="BlpR"
                     /note="confers resistance to bialophos or
                     phosphinothricin"
                     /note="color: #ccffcc"
                     /protein_id=""
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQ
                     EWTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGL
                     GSTLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHD
                     VGFWQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /note="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
                     /note="color: #ffffff; direction: LEFT"
     promoter        2305..2650
                     /note="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower
                     mosaic virus"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    2680..2734
                     /note="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic
                     virus 5'-leader sequence (Gallie et al., 1988)"
                     /note="color: #a6acb3"
     misc_feature    3270..4016
                     /note="2X35S"
                     /note="color: #ff00ff"
     promoter        3971..4017
                     /note="minimal CaMV 35S promoter"
                     /note="minimal 35S promoter from cauliflower mosaic virus"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4076..4786
                     /dnas_title="monomeric derivative of DsRed fluorescent
                     protein (Shaner et al., 2004)"
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent
                     protein (Shaner et al., 2004)"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK"
     misc_feature    4787..4801
                     /note="HDEL"
                     /note="color: #000000"
     misc_feature    4802..4976
                     /note="35S terminator"
                     /note="color: #a6acb3"
     polyA_signal    4802..4976
                     /note="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    4977..5723
                     /note="2X35S"
                     /note="color: #ff00ff"
     promoter        5678..5724
                     /note="minimal CaMV 35S promoter"
                     /note="minimal 35S promoter from cauliflower mosaic virus"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    5724..5850
                     /note="TEV"
                     /note="color: #0000ff"
     PCR_primer      5853..5875
                     /note="sense primer for dspAvr2 coloning"
                     /current=0
     misc_feature    5853..5906
                     /note="37 truncation editon"
     misc_feature    5853..6284
                     /note="dspAVR2"
     PCR_primer      5853..5875
                     /note="sense primer for dspAvr2 coloning"
                     /current=0
     misc_feature    6045..6058
                     /note="RIYER"
     PCR_primer      complement(6257..6284)
                     /note="anti-sense primer"
                     /current=0
     PCR_primer      complement(6257..6284)
                     /note="anti-sense primer"
                     /current=0
     misc_feature    6045..6058
                     /note="RIYER"
     PCR_primer      complement(6260..6284)
                     /pair=""
                     /primer="ATCCTCTGAGATAGTAAGATAGTAG"
                     /current=1
     PCR_primer      6014..6031
                     /primer="TGAGCGGGCTGGCAATTC"
                     /note="962"
                     /set="Set 2"
     PCR_primer      complement(6014..6031)
                     /primer="GAATTGCCAGCCCGCTCA"
                     /note="1044"
                     /set="Set 2"
     PCR_primer      complement(6076..6094)
                     /primer="ACATCAATGACAGTGCGGA"
                     /note="1067"
                     /set="Set 2"
     CDS             6291..7010
                     /dnas_title="enhanced GFP"
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments: 1: 6760 .. 6762 /
                     #05fd14 2: 6763 .. 6765 / #05fd14 / 1a 3: 6766 .. 7479 /
                     #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
     misc_feature    7011..7185
                     /note="35S terminator"
                     /note="color: #a6acb3"
     polyA_signal    7011..7185
                     /note="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(7195..7211)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    7414..7438
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     CDS             8738..9367
                     /dnas_title="stability protein from Pseudomonas plasmid
                     pVS1"
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /note="pVS1 StaA"
                     /note="color: #993366"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSA
                     RDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVL
                     IPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYE
                     LPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9796..10869
                     /dnas_title="replication protein from Pseudomonas plasmid
                     pVS1"
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid
                     pVS1"
                     /note="pVS1 RepA"
                     /note=""
                     /note="color: #993366"
                     /protein_id=""
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGES
                     WQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFR
                     LSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKG
                     DKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTV
                     SEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIG
                     RFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEA
                     GQAGPILVMRYRNLIEGEASAGS"
     misc_feature    11473..11613
                     /note="bom"
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     rep_origin      complement(11799..12387)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
     CDS             complement(12474..13268)
                     /dnas_title="aminoglycoside phosphotransferase"
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /protein_id=""
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     source          6291..13692
                     /dnas_title="pZK538-dspAVR2-GFP"
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN      
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agttagatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactag ctcagatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagactcc tttgccccgg agattacaat ggacgatttc
     1741 ctctatcttt acgatctagg aaggaagttc gaaggtgaag gtgacgacac tatgttcacc
     1801 actgataatg agaaggttag cctcttcaat ttcagaaaga atgctgaccc acagatggtt
     1861 agagaggcct acgcagcagg tctcatcaag acgatctacc cgagtaacaa tctccaggag
     1921 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taattgcatc
     1981 aagaacacag agaaagacat atttctcaag atcagaagta ctattccagt atggacgatt
     2041 caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
     2101 cctactgaat ctaaggccat gcatggagtc taagattcaa atcgaggatc taacagaact
     2161 cgccgtgaag actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa
     2221 aatcttcgtc aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac
     2281 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct
     2341 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
     2401 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc
     2461 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
     2521 tccaaccacg tcttcaaagc aagtggattg atgtgacatc tccactgacg taagggatga
     2581 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     2641 ggagaggaca cgctcgagta taagagctct atttttacaa caattaccaa caacaacaaa
     2701 caacaaacaa cattacaatt acatttacaa ttaccatggg gcgcgcccaa tcgatgattt
     2761 aaatgtgtaa gaatttctta tgttacatta ttacattcaa cgttttatct taattggctc
     2821 ttcatttgat tgaaatttga caattatttc ttgttttttt ttttgtcaca ctctttttgg
     2881 gttggggtgg ccgacgaatt gtgggaaggt agaaagaggg gaggactttt gttatactcc
     2941 attagtaatt actgtttccg tttcaattta tgtgacaata tttccttttt agtcggttcc
     3001 aaaagaaaat gtcagcatta taaacaattt aattttgaaa ttacaatttt gccattaata
     3061 aaatgattta caaccacaaa agtatctatg agcctgtttg ggtgggctta taagcagctt
     3121 attttaagtg gcttataagt caaaaagtga cantttttga gaagttagaa aatcctaact
     3181 tctcaaaaag tagcttttaa gccacttatg acttataagt ccaaaaattt ttaagttacc
     3241 aaacatatat taatgggttt ataagcttaa acatggtgga gcacgacact ctcgtctact
     3301 ccaagaatat caaagataca gtctcagaag accagagggc tattgagact tttcaacaaa
     3361 gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa
     3421 ggacagtaga aaaggaagat ggcttctaca aatgccatca ttgcgataaa ggaaaggcta
     3481 tcgttcaaag aatgcctcta ccgacagtgg tcccaaagat ggacccccca cccacgagga
     3541 acatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
     3601 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
     3661 accagagggc tattgagact ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca
     3721 ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaagatg gcttctacaa
     3781 atgccatcat tgcgataaag gaaaggctat cgttcaagaa tgcctctacc gacagtggtc
     3841 ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt
     3901 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     3961 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggatga
     4021 agactaatct ttttctcttt ctcatctttt cacttctcct atcattatcc tcggcatggt
     4081 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     4141 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     4201 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     4261 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     4321 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     4381 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     4441 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     4501 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     4561 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     4621 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     4681 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     4741 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaacatg atgagcttta
     4801 atttctccat aataatgtgt gagtagttcc cagataaggg aattagggtt cctatagggt
     4861 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     4921 tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatcaaca
     4981 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
     5041 agagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
     5101 gcccagctat ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc ttctacaaat
     5161 gccatcattg cgataaagga aaggctatcg ttcaaagaat gcctctaccg acagtggtcc
     5221 caaagatgga ccccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt
     5281 cttcaaagca agtggattga tgtgataaca tggtggagca cgacactctc gtctactcca
     5341 agaatatcaa agatacagtc tcagaagacc agagggctat tgagactttc aacaaagggt
     5401 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac
     5461 agtagaaaag gaagatggct tctacaaatg ccatcattgc gataaaggaa aggctatcgt
     5521 tcaagaatgc ctctaccgac agtggtccca aagatggacc cccacccacg aggaacatcg
     5581 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca
     5641 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
     5701 gaagttcatt tcatttggag aggaacacaa catatacaaa acaaacgaat ctcaagcaat
     5761 caagcattct acttctattg cagcaattta aatcatttct tttaaagcaa aagcaatttt
     5821 ctgaaaattt tcaccattta cgaacgatag ccATGcctgt ggaagatgcc gattcatctg
     5881 tcggtcagct ccaaggacgg ggtaacccat attgcgtgtt tcccggccgc cccacgtctt
     5941 ctacttcatt tactacgagc ttcagcaccg aacctctggg ttatgctaga atgttacaca
     6001 gagaccctcc atatgagcgg gctggcaatt cgggactgaa tcaccgcatt tacgaacgaa
     6061 gtcgcgttgg aggcctccgc actgtcattg atgtcgcccc cccggacgga caccaagcca
     6121 tcgccaacta cgagatcgaa gtccgtcgta ttcctgttgc aacacctaat gcggctggag
     6181 attgctttca cactgcacgc cttagtaccg gctctcgagg tccagccaca atcagttggg
     6241 atgccgacgc tagttatacc tactatctta ctatctcaga ggatggatcc atggtgagca
     6301 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     6361 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     6421 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     6481 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     6541 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     6601 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     6661 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     6721 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     6781 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     6841 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     6901 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     6961 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa tttctccata
     7021 ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt
     7081 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
     7141 aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcaagct tggcactggc
     7201 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     7261 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     7321 ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg atcagattgt
     7381 cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct
     7441 aagagaaaag agcgtttatt agaataacgg atatttaaaa gggcgtgaaa aggtttatcc
     7501 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
     7561 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
     7621 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
     7681 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
     7741 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
     7801 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
     7861 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
     7921 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
     7981 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
     8041 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
     8101 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
     8161 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
     8221 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
     8281 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
     8341 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
     8401 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
     8461 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
     8521 tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
     8581 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
     8641 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
     8701 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
     8761 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
     8821 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
     8881 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
     8941 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
     9001 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
     9061 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
     9121 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
     9181 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
     9241 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
     9301 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
     9361 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
     9421 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
     9481 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
     9541 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
     9601 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
     9661 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
     9721 ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa
     9781 gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg
     9841 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc
     9901 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa
     9961 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag
    10021 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc
    10081 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc
    10141 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg
    10201 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac
    10261 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc
    10321 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt
    10381 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg
    10441 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc
    10501 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa
    10561 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
    10621 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
    10681 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
    10741 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
    10801 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
    10861 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
    10921 aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
    10981 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
    11041 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
    11101 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg
    11161 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
    11221 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
    11281 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc
    11341 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
    11401 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    11461 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
    11521 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
    11581 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
    11641 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    11701 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    11761 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    11821 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    11881 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    11941 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    12001 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    12061 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    12121 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    12181 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
    12241 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    12301 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    12361 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    12421 gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac
    12481 aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag
    12541 tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag
    12601 aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt
    12661 actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt
    12721 tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga
    12781 gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc
    12841 aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag
    12901 tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct
    12961 tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca
    13021 tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc
    13081 atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt
    13141 aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc
    13201 gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt
    13261 ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
    13321 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
    13381 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
    13441 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
    13501 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
    13561 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
    13621 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
    13681 ttggctggct gg
//

