LOCUS       Exported               14161 bp ds-DNA    circular SYN 07-¾ÅÔÂ-2017
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pFGC5941-2X35S-mCherry-HDEL-35S ter-2X35S-TEV-TSWV NSm-EGFP-35S ...
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14161)
  AUTHORS   wifi
  TITLE     Direct Submission
  JOURNAL   Exported 2017Äê9ÔÂ7ÈÕ from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14169
                     /dnas_title="Exported"
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..25
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     terminator      111..363
                     /note="MAS terminator"
                     /note="mannopine synthase terminator"
                     /note="color: #ffffff"
     CDS             complement(373..924)
                     /dnas_title="phosphinothricin acetyltransferase"
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /function=""
                     /note="BlpR"
                     /note="confers resistance to bialophos or
                     phosphinothricin"
                     /note="color: #ccffcc"
                     /protein_id=""
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQ
                     EWTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGL
                     GSTLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHD
                     VGFWQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /note="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
                     /note="color: #ffffff; direction: LEFT"
     promoter        2305..2650
                     /note="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower
                     mosaic virus"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    2680..2734
                     /note="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic
                     virus 5'-leader sequence (Gallie et al., 1988)"
                     /note="color: #a6acb3"
     misc_feature    3270..4016
                     /note="2X35S"
                     /note="color: #ff00ff"
     promoter        3971..4017
                     /note="minimal CaMV 35S promoter"
                     /note="minimal 35S promoter from cauliflower mosaic virus"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             4076..4786
                     /dnas_title="monomeric derivative of DsRed fluorescent
                     protein (Shaner et al., 2004)"
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent
                     protein (Shaner et al., 2004)"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK"
     misc_feature    4787..4801
                     /note="HDEL"
                     /note="color: #000000"
     misc_feature    4802..4976
                     /note="35S terminator"
                     /note="color: #a6acb3"
     polyA_signal    4802..4976
                     /note="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    4977..5723
                     /note="2X35S"
                     /note="color: #ff00ff"
     promoter        5678..5724
                     /note="minimal CaMV 35S promoter"
                     /note="minimal 35S promoter from cauliflower mosaic virus"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    5724..5850
                     /note="TEV"
                     /note="color: #0000ff"
     misc_feature    5853..6761
                     /note="TSWV-NSm"
                     /note="color: #00ccff"
     CDS             6768..7487
                     /dnas_title="enhanced GFP"
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments: 1: 6760 .. 6762 /
                     #05fd14 2: 6763 .. 6765 / #05fd14 / 1a 3: 6766 .. 7479 /
                     #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
     misc_feature    7488..7662
                     /note="35S terminator"
                     /note="color: #a6acb3"
     polyA_signal    7488..7662
                     /note="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(7672..7688)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    7891..7915
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     CDS             9215..9844
                     /dnas_title="stability protein from Pseudomonas plasmid
                     pVS1"
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /note="pVS1 StaA"
                     /note="color: #993366"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSA
                     RDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVL
                     IPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYE
                     LPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10273..11346
                     /dnas_title="replication protein from Pseudomonas plasmid
                     pVS1"
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid
                     pVS1"
                     /note="pVS1 RepA"
                     /note=""
                     /note="color: #993366"
                     /protein_id=""
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGES
                     WQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFR
                     LSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKG
                     DKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTV
                     SEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIG
                     RFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEA
                     GQAGPILVMRYRNLIEGEASAGS"
     misc_feature    11950..12090
                     /note="bom"
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     rep_origin      complement(12276..12864)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /note="color: #ffff00"
     CDS             complement(12951..13745)
                     /dnas_title="aminoglycoside phosphotransferase"
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /protein_id=""
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     PCR_primer      5619..5639
                     /primer="GTGGATTGATGTGATATCTCC"
                     /note="8"
                     /set="Set 1"
     PCR_primer      3912..3932
                     /primer="GTGGATTGATGTGATATCTCC"
                     /note="8"
                     /set="Set 1"
     PCR_primer      complement(7687..7705)
                     /primer="TTCCCAGTCACGACGTTGT"
                     /note="11"
                     /set="Set 1"
     PCR_primer      32..58
                     /primer="TAAAGCTTACAACTTAATAACACATTG"
                     /note="63"
                     /set="Set 1"
     PCR_primer      11869..11886
                     /primer="GGTCACAGCTTGTCTGTA"
                     /note="168"
                     /set="Set 1"
     PCR_primer      6910..6936
                     /primer="CCGAATTCGTATACGAGCTGCCCGTGC"
                     /note="190"
                     /set="Set 1"
     PCR_primer      1843..1864
                     /primer="GCTAACCCACAGATGGTTAGAG"
                     /note="195"
                     /set="Set 1"
     PCR_primer      5646..5665
                     /primer="GTAAGGGATGACGCACAATC"
                     /note="403"
                     /set="Set 1"
     PCR_primer      3939..3958
                     /primer="GTAAGGGATGACGCACAATC"
                     /note="403"
                     /set="Set 1"
     PCR_primer      2570..2589
                     /primer="GTAAGGGATGACGCACAATC"
                     /note="403"
                     /set="Set 1"
     PCR_primer      5668..5686
                     /primer="ACTATCCTTCGCAAGACCC"
                     /note="404"
                     /set="Set 1"
     PCR_primer      3961..3979
                     /primer="ACTATCCTTCGCAAGACCC"
                     /note="404"
                     /set="Set 1"
     PCR_primer      2592..2610
                     /primer="ACTATCCTTCGCAAGACCC"
                     /note="404"
                     /set="Set 1"
     PCR_primer      complement(4765..4806)
                     /primer="CGGGATCCTTAAAGCTCATCATGCTTGTACAGCTCGTCCATG"
                     /note="445"
                     /set="Set 1"
     PCR_primer      complement(7466..7507)
                     /primer="CGGGATCCTTAAAGCTCATCATGCTTGTACAGCTCGTCCATG"
                     /note="445"
                     /set="Set 1"
     PCR_primer      6741..6784
                     /primer="AATATCGATTGGAAATATATTATGGTGAGCAAGGGCGA"
                     /note="479"
                     /set="Set 1"
     PCR_primer      4055..4092
                     /primer="AATATCGATTGGAAATATATTATGGTGAGCAAGGGCGA"
                     /note="479"
                     /set="Set 1"
     PCR_primer      complement(4767..4794)
                     /primer="ATGGATCCTTACTTGTACAGCTCGTCCA"
                     /note="481"
                     /set="Set 1"
     PCR_primer      complement(7468..7495)
                     /primer="ATGGATCCTTACTTGTACAGCTCGTCCA"
                     /note="481"
                     /set="Set 1"
     PCR_primer      6753..6788
                     /primer="AAAGGGCCCATGGTGAGCAAGGGCGAGGAG"
                     /note="732"
                     /set="Set 1"
     PCR_primer      4067..4096
                     /primer="AAAGGGCCCATGGTGAGCAAGGGCGAGGAG"
                     /note="732"
                     /set="Set 1"
     PCR_primer      complement(4768..4795)
                     /primer="AAAGGGCCCTTACTTGTACAGCTCGTCC"
                     /note="733"
                     /set="Set 1"
     PCR_primer      complement(7469..7496)
                     /primer="AAAGGGCCCTTACTTGTACAGCTCGTCC"
                     /note="733"
                     /set="Set 1"
     PCR_primer      6753..6788
                     /primer="AAATCTAGAATGGTGAGCAAGGGCGAGGAG"
                     /note="740"
                     /set="Set 1"
     PCR_primer      4067..4096
                     /primer="AAATCTAGAATGGTGAGCAAGGGCGAGGAG"
                     /note="740"
                     /set="Set 1"
     PCR_primer      complement(4768..4795)
                     /primer="AAATCTAGATTACTTGTACAGCTCGTCC"
                     /note="741"
                     /set="Set 1"
     PCR_primer      complement(7469..7496)
                     /primer="AAATCTAGATTACTTGTACAGCTCGTCC"
                     /note="741"
                     /set="Set 1"
     PCR_primer      7668..7691
                     /primer="TGGCACTGGCCGTCGTTTTACAAC"
                     /note="742"
                     /set="Set 1"
     PCR_primer      7689..7711
                     /primer="AACGTCGTGACTGGGAAAACCCT"
                     /note="743"
                     /set="Set 1"
     PCR_primer      7792..7811
                     /primer="CCCTTCCCAACAGTTGCGCA"
                     /note="744"
                     /set="Set 1"
     PCR_primer      complement(7792..7811)
                     /primer="TGCGCAACTGTTGGGAAGGG"
                     /note="746"
                     /set="Set 1"
     PCR_primer      complement(75..97)
                     /primer="GAGCAATTCGGCGTTAATTCAGT"
                     /note="749"
                     /set="Set 1"
     PCR_primer      6753..6787
                     /primer="AAAGGGCCCATGGTGAGCAAGGGCGAGGA"
                     /note="884"
                     /set="Set 1"
     PCR_primer      4067..4095
                     /primer="AAAGGGCCCATGGTGAGCAAGGGCGAGGA"
                     /note="884"
                     /set="Set 1"
     PCR_primer      complement(4766..4795)
                     /primer="AAAGGGCCCTTACTTGTACAGCTCGTCCAT"
                     /note="885"
                     /set="Set 1"
     PCR_primer      complement(7467..7496)
                     /primer="AAAGGGCCCTTACTTGTACAGCTCGTCCAT"
                     /note="885"
                     /set="Set 1"
     PCR_primer      complement(4084..4102)
                     /primer="GAACAGCTCCTCGCCCTTG"
                     /note="891"
                     /set="Set 1"
     PCR_primer      complement(6776..6794)
                     /primer="GAACAGCTCCTCGCCCTTG"
                     /note="891"
                     /set="Set 1"
     PCR_primer      complement(7721..7745)
                     /primer="GATGTGCTGCAAGGCGATTAAGTTG"
                     /note="1114"
                     /set="Set 1"
     PCR_primer      6753..6787
                     /primer="AAAGGGCCCATGGTGAGCAAGGGCGAGGA"
                     /note="1122"
                     /set="Set 1"
     PCR_primer      4067..4095
                     /primer="AAAGGGCCCATGGTGAGCAAGGGCGAGGA"
                     /note="1122"
                     /set="Set 1"
     PCR_primer      complement(4764..4792)
                     /primer="AAAACTAGTCTTGTACAGCTCGTCCATGC"
                     /note="1123"
                     /set="Set 1"
     PCR_primer      complement(7465..7493)
                     /primer="AAAACTAGTCTTGTACAGCTCGTCCATGC"
                     /note="1123"
                     /set="Set 1"
     PCR_primer      7488..7509
                     /pair=""
                     /primer="TTTCTCCATAATAATGTGTGAG"
                     /current=0
     PCR_primer      complement(5834..5852)
                     /pair=""
                     /primer="GGCTATCGTTCGTAAATGG"
                     /current=1
ORIGIN      
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agttagatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactag ctcagatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagactcc tttgccccgg agattacaat ggacgatttc
     1741 ctctatcttt acgatctagg aaggaagttc gaaggtgaag gtgacgacac tatgttcacc
     1801 actgataatg agaaggttag cctcttcaat ttcagaaaga atgctgaccc acagatggtt
     1861 agagaggcct acgcagcagg tctcatcaag acgatctacc cgagtaacaa tctccaggag
     1921 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taattgcatc
     1981 aagaacacag agaaagacat atttctcaag atcagaagta ctattccagt atggacgatt
     2041 caaggcttgc ttcataaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
     2101 cctactgaat ctaaggccat gcatggagtc taagattcaa atcgaggatc taacagaact
     2161 cgccgtgaag actggcgaac agttcataca gagtctttta cgactcaatg acaagaagaa
     2221 aatcttcgtc aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac
     2281 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct
     2341 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
     2401 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc
     2461 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
     2521 tccaaccacg tcttcaaagc aagtggattg atgtgacatc tccactgacg taagggatga
     2581 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     2641 ggagaggaca cgctcgagta taagagctct atttttacaa caattaccaa caacaacaaa
     2701 caacaaacaa cattacaatt acatttacaa ttaccatggg gcgcgcccaa tcgatgattt
     2761 aaatgtgtaa gaatttctta tgttacatta ttacattcaa cgttttatct taattggctc
     2821 ttcatttgat tgaaatttga caattatttc ttgttttttt ttttgtcaca ctctttttgg
     2881 gttggggtgg ccgacgaatt gtgggaaggt agaaagaggg gaggactttt gttatactcc
     2941 attagtaatt actgtttccg tttcaattta tgtgacaata tttccttttt agtcggttcc
     3001 aaaagaaaat gtcagcatta taaacaattt aattttgaaa ttacaatttt gccattaata
     3061 aaatgattta caaccacaaa agtatctatg agcctgtttg ggtgggctta taagcagctt
     3121 attttaagtg gcttataagt caaaaagtga caatttttga gaagttagaa aatcctaact
     3181 tctcaaaaag tagcttttaa gccacttatg acttataagt ccaaaaattt ttaagttacc
     3241 aaacatatat taatgggttt ataagcttaa acatggtgga gcacgacact ctcgtctact
     3301 ccaagaatat caaagataca gtctcagaag accagagggc tattgagact tttcaacaaa
     3361 gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa
     3421 ggacagtaga aaaggaagat ggcttctaca aatgccatca ttgcgataaa ggaaaggcta
     3481 tcgttcaaag aatgcctcta ccgacagtgg tcccaaagat ggacccccca cccacgagga
     3541 acatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
     3601 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
     3661 accagagggc tattgagact ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca
     3721 ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaagatg gcttctacaa
     3781 atgccatcat tgcgataaag gaaaggctat cgttcaagaa tgcctctacc gacagtggtc
     3841 ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt
     3901 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     3961 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggatga
     4021 agactaatct ttttctcttt ctcatctttt cacttctcct atcattatcc tcggcatggt
     4081 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     4141 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     4201 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     4261 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     4321 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     4381 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     4441 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     4501 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     4561 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     4621 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     4681 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     4741 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaacatg atgagcttta
     4801 atttctccat aataatgtgt gagtagttcc cagataaggg aattagggtt cctatagggt
     4861 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     4921 tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatcaaca
     4981 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
     5041 agagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
     5101 gcccagctat ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc ttctacaaat
     5161 gccatcattg cgataaagga aaggctatcg ttcaaagaat gcctctaccg acagtggtcc
     5221 caaagatgga ccccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt
     5281 cttcaaagca agtggattga tgtgataaca tggtggagca cgacactctc gtctactcca
     5341 agaatatcaa agatacagtc tcagaagacc agagggctat tgagactttc aacaaagggt
     5401 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac
     5461 agtagaaaag gaagatggct tctacaaatg ccatcattgc gataaaggaa aggctatcgt
     5521 tcaagaatgc ctctaccgac agtggtccca aagatggacc cccacccacg aggaacatcg
     5581 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca
     5641 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
     5701 gaagttcatt tcatttggag aggaacacaa catatacaaa acaaacgaat ctcaagcaat
     5761 caagcattct acttctattg cagcaattta aatcatttct tttaaagcaa aagcaatttt
     5821 ctgaaaattt tcaccattta cgaacgatag ccatgttgac tttttttggt aataaggggt
     5881 cttctaagtc tgccagaaag gatgaagggc ctttagtttc acttgctaaa cataacggta
     5941 atgttgaagt ctcaaaacca tggtcttctt ctgatgaaaa gcttgcttta accaaagcta
     6001 tggatacatc caaaggaaag atactgttga acacagaggg aacatcttcc tttggaacat
     6061 atgaatctga ttctatcaca gaatcagagg gttatgatct ttctgcgaga atgatagtag
     6121 atacaaacca ccatatctca aactggaaaa atgatctttt tgtcggcaac gggaagcaaa
     6181 acgctaataa ggtcatcaag atctgtccaa cttgggacag cagaaaacaa tacatgatga
     6241 tttccaggat tgtgatatgg gtctgcccca ctataccaaa ccctacaggg aaacttgtgg
     6301 ttgctctggt cgatcccaac atgccatctg aaaagcaaat cattctgaag ggtcagggga
     6361 caataactga tcctatctgt tttgtttttt atctgaactg gtctattccg aaaatgaata
     6421 acactccaga aaactgctgt cagctgcact tgatgtgcag tcaagaatac aagaaggggg
     6481 tttcttttgg tagtgtcatg tattcttgga caaaggagtt ttgtgattca cccagagctg
     6541 ataaagacaa aagttgcatg gtcatacctc taaacagggc tattagagct agatctcaag
     6601 cattcattga ggcttgcaag ctgataattc ctaaaggaaa cagtgagaag cagattaaaa
     6661 aacagcttaa agaactgagc tcaaatcttg agagatcagt tgaagaagag gaggaagggg
     6721 tttatgataa tgttgctcag ttatcttttg atgagatata gggatccatg gtgagcaagg
     6781 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     6841 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     6901 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     6961 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     7021 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     7081 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     7141 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     7201 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     7261 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     7321 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     7381 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     7441 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaattt ctccataata
     7501 atgtgtgagt agttcccaga taagggaatt agggttccta tagggtttcg ctcatgtgtt
     7561 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
     7621 ttctaattcc taaaaccaaa atccagtact aaaatccaga tcaagcttgg cactggccgt
     7681 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     7741 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     7801 acagttgcgc agcctgaatg gcgaatgcta gagcagcttg agcttggatc agattgtcgt
     7861 ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
     7921 agaaaagagc gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt
     7981 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
     8041 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa
     8101 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
     8161 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
     8221 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
     8281 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
     8341 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
     8401 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
     8461 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
     8521 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
     8581 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
     8641 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
     8701 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
     8761 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
     8821 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
     8881 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
     8941 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
     9001 agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
     9061 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
     9121 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
     9181 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
     9241 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
     9301 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
     9361 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
     9421 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
     9481 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
     9541 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
     9601 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
     9661 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
     9721 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
     9781 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
     9841 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
     9901 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
     9961 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
    10021 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
    10081 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
    10141 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
    10201 gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc
    10261 cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
    10321 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
    10381 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
    10441 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
    10501 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
    10561 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
    10621 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
    10681 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
    10741 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
    10801 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
    10861 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
    10921 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
    10981 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
    11041 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
    11101 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
    11161 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
    11221 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
    11281 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
    11341 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
    11401 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
    11461 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
    11521 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
    11581 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
    11641 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
    11701 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
    11761 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
    11821 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
    11881 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    11941 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
    12001 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
    12061 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
    12121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    12181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    12241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    12301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    12361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    12421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    12481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    12541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    12601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    12661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
    12721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    12781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    12841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    12901 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
    12961 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
    13021 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
    13081 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
    13141 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
    13201 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
    13261 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
    13321 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
    13381 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
    13441 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
    13501 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
    13561 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
    13621 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
    13681 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
    13741 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
    13801 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
    13861 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
    13921 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
    13981 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
    14041 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
    14101 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
    14161 gctggctgg
//

