LOCUS       Exported                2653 bp ds-DNA     circular SYN 21-SEP-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Alejandro Sarrion-Perdigones
  TITLE     Direct Submission
  JOURNAL   Exported Sep 21, 2018 from SnapGene 4.2.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        34..81
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
                     /note="color: #ffffff; direction: RIGHT"
     CDS             113..400
                     /codon_start=1
                     /label=LacZ
                     /note="color: #0000ff"
                     /translation="MTMITPSYLGDTIEYSSLHACRSTLEDPRVPSSNSPYSESYYNSL
                     AVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGE"
     promoter        135..153
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    160..216
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
                     /note="color: #99ccff"
     promoter        complement(219..237)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(244..260)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      complement(602..1284)
                     /direction=LEFT
                     /label=ColE1 origin
                     /note="color: #ffff00"
     promoter        1323..1421
                     /label=cat Promoter
                     /note="color: #ffffff"
     CDS             1426..2082
                     /codon_start=1
                     /label=Chloramphenicol Resistance
                     /note="color: #ff6600"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      2086..2193
                     /note="color: #ffffff"
     rep_origin      2198..2653
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
ORIGIN
        1 taactgcagg tctcagtcag gagagagacg cctgttgaca attaatcatc ggcatagtat
       61 atcggcatag tataatacga caaggtgagg aactaaaccc aggaggcaga tcatgaccat
      121 gattacgcca agctatttag gtgacactat agaatactca agcttgcatg cctgcaggtc
      181 gactctagag gatccccggg taccgagctc gaattcgccc tatagtgagt cgtattacaa
      241 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      301 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      361 tcgcccttcc caacagttgc gcagcctgaa tggcgaatga cgcctgatgc ggtattttct
      421 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
      481 tgatgccgca tagcgtctct cgcttgagac ctgcagtgac acggttatcc acagaatcag
      541 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      601 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      661 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      721 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      781 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      841 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      901 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      961 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1021 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1081 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1141 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1201 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1261 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttgt
     1321 gatgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
     1381 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     1441 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     1501 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     1561 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     1621 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     1681 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     1741 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     1801 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtg
     1861 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     1921 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     1981 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     2041 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     2101 tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa gcggatgaat
     2161 ggcagaaatt cgatgataag ctgtcaaaca tgatgttacg cgccctgtag cggcgcatta
     2221 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     2281 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     2341 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     2401 aaaaaacttg attagggtga tggttcacgt agtgggccag cgccctgata gacggttttt
     2461 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     2521 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     2581 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     2641 acgcttacaa ttt
//
