LOCUS       Exported                2385 bp ds-DNA     circular SYN 10-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Dec 10, 2018 from SnapGene 4.2.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     1..32
                     /label=NM15OCT29
     primer_bind     20..36
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    109..114
                     /label=E-BOX
                     /bound_moiety="MYC"
     protein_bind    117..122
                     /label=E-BOX
                     /bound_moiety="MYC"
     protein_bind    125..130
                     /label=E-BOX
                     /bound_moiety="MYC"
     protein_bind    133..138
                     /label=E-BOX
                     /bound_moiety="MYC"
     protein_bind    141..146
                     /label=E-BOX
                     /bound_moiety="MYC"
     primer_bind     151..177
                     /label=NM15MAY02
     primer_bind     151..177
                     /label=NM15MAY01
     promoter        complement(187..205)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(221..237)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(235..252)
                     /label=NM15OCT30
     primer_bind     249..272
                     /label=ASP14NOV50
     rep_origin      complement(334..1016)
                     /direction=LEFT
                     /label=ColE1 origin
     primer_bind     complement(1029..1054)
                     /label=ASP14NOV51
     promoter        1055..1153
                     /label=cat Promoter
     CDS             1158..1814
                     /codon_start=1
                     /label=Chloramphenicol Resistance
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1818..1925
     primer_bind     1926..1949
                     /label=ASP15JAN01
     rep_origin      1930..2385
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(join(2354..2385,1..4))
                     /label=ASP15JUL28
ORIGIN
        1 taacattgta gaaacgcatg taaaacgacg gccagtgaat tgtaatacga ctcactatag
       61 ggtgaattcc gcttaggacc ttagatattg atctcaaggt ctcggggaca cgtgagcacg
      121 tgagcacgtg tccacgtgca cacgtgctcc cgagaccact catcgcccaa tcactagtga
      181 attcgttcta tagtgtcacc taaataccat agagccgttg gtcatagctg tttcctgtcc
      241 aggcatcatg acacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      301 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      361 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      421 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      481 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      541 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      601 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      661 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      721 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      781 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      841 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      901 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
      961 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1021 atcaaaaagg atcttcacct agatcctttt gtgatgatcg gcacgtaaga ggttccaact
     1081 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag
     1141 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     1201 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     1261 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     1321 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
     1381 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     1441 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     1501 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     1561 ctaaagggtt tattgagaat atgtttttcg tgtcagccaa tccctgggtg agtttcacca
     1621 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     1681 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     1741 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     1801 ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggttgct
     1861 acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgatgata agctgtcaaa
     1921 catgatgtta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     1981 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     2041 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     2101 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     2161 gtagtgggcc agcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     2221 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     2281 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     2341 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aattt
//
