LOCUS       Exported                2401 bp ds-DNA     circular SYN 10-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Dec 10, 2018 from SnapGene 4.2.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     20..36
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    108..162
                     /label=RE
                     /bound_moiety="p53"
                     /note="p53 response element created by fusing two human p53
                     binding sites"
     promoter        complement(203..221)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(237..253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(350..1032)
                     /direction=LEFT
                     /label=ColE1 origin
     promoter        1071..1169
                     /label=cat Promoter
     CDS             1174..1830
                     /codon_start=1
                     /label=Chloramphenicol Resistance
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1834..1941
     rep_origin      1946..2401
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 taacattgta gaaacgcatg taaaacgacg gccagtgaat tgtaatacga ctcactatag
       61 ggtgaattcc gcttaggacc ttagatattg atctcaaggt ctcggggaag gcaaggccag
      121 gcaagtccag gcaaggcaca gcatgcttag acatgttctg tactcccgag accactcatc
      181 gcccaatcac tagtgaattc gttctatagt gtcacctaaa taccatagag ccgttggtca
      241 tagctgtttc ctgtccaggc atcatgacac ggttatccac agaatcaggg gataacgcag
      301 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      361 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      421 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      481 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      541 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      601 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      661 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      721 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      781 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      841 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      901 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      961 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1021 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttgtga tgatcggcac
     1081 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
     1141 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     1201 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     1261 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     1321 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     1381 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     1441 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     1501 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     1561 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtgtc agccaatccc
     1621 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     1681 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     1741 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     1801 cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct
     1861 taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg
     1921 atgataagct gtcaaacatg atgttacgcg ccctgtagcg gcgcattaag cgcggcgggt
     1981 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     2041 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     2101 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     2161 tagggtgatg gttcacgtag tgggccagcg ccctgataga cggtttttcg ccctttgacg
     2221 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     2281 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     2341 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     2401 t
//
