LOCUS       Exported                4097 bp ds-DNA     circular SYN 10-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Dec 10, 2018 from SnapGene 4.2.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        86..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             623..1558
                     /codon_start=1
                     /label=GreenRenilla_HS
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNATSSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGSALAFHYAYEHQDRIKAIVHMESVVDVIESWMGWPDIEEEL
                     ALIKSEEGEKMVLENNFFVETLLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKLFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFLQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    1563..1787
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1889..2571)
                     /direction=LEFT
                     /label=ColE1 origin
     promoter        2610..2708
                     /label=cat Promoter
     CDS             2713..3525
                     /codon_start=1
                     /label=Kanamycin Resistance
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      3530..3637
     rep_origin      3642..4097
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 taacaagctt cgtctcagtc aggagtagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
      601 tgaaccgtca gatcaagctc aaatggcttc caaagtctac gacccagaac aaaggaagag
      661 gatgattact ggaccgcagt ggtgggcaag gtgcaagcag atgaatgtac tcgatagttt
      721 tatcaattat tacgactccg aaaaacacgc ggaaaatgct gtcatattcc tgcacgggaa
      781 cgccactagc agctacttgt ggcggcacgt agtcccccac atagagcctg ttgcccgatg
      841 tattattccc gatctcattg gaatgggcaa atcaggcaaa tctgggaacg gttcttaccg
      901 cctgctcgat cactataaat atttgaccgc ttggtttgag ctgcttaatc tccctaaaaa
      961 aataatattc gttggacacg actggggctc agctctggcg ttccactacg catacgagca
     1021 tcaagaccga ataaaagcaa tagtacatat ggagtcagtt gttgacgtca tagagtcatg
     1081 gatgggttgg ccagatattg aagaggaatt ggccctcatc aagagtgagg agggggaaaa
     1141 aatggtcctg gaaaataatt ttttcgtgga aaccttgctc ccgagtaaaa taatgcgaaa
     1201 acttgaaccc gaagagtttg ctgcttattt ggagcctttc aaggaaaagg gggaagtaag
     1261 acgaccaacc ctttcttggc cgagggagat acctcttgtt aagggtggaa agcccgacgt
     1321 agtccagata gtccgaaatt acaacgcgta cctgagagct agtgatgacc tccctaagtt
     1381 gtttatcgag tcagaccctg ggttcttcag caacgcaatt gtagaaggag cgaaaaaatt
     1441 tccaaataca gaatttgtga aggttaaggg gctccacttt ttgcaagagg acgcccccga
     1501 tgagatggga aaatacatta aatctttcgt agagcgcgtc cttaagaatg aacagtgagc
     1561 ttctgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     1621 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     1681 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     1741 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggcgc ttgagacgaa
     1801 gcttgacacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1861 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1921 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1981 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2041 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2101 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2161 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2221 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2281 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2341 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2401 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2461 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2521 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2581 aaaggatctt cacctagatc cttttgtgat gatcggcacg taagaggttc caactttcac
     2641 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     2701 aaggaagcta aaatgagcca tattcaacgg gaaacgtctt gctcaaggcc gcgattaaat
     2761 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     2821 ggtgcgacaa tctaccgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     2881 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     2941 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     3001 ctcaccactg cgatcccagg gaaaacagca ttccaggtat tagaagaata tcctgattca
     3061 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     3121 tgtaattgtc cttttaacag cgatcgcgta tttcgactcg ctcaggcgca atcacgaatg
     3181 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     3241 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     3301 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     3361 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     3421 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     3481 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctgatt ttttttaagg
     3541 cagttattgg tgcccttaaa cgcctggttg ctacgcctga ataagtgata ataagcggat
     3601 gaatggcaga aattcgatga taagctgtca aacatgatgt tacgcgccct gtagcggcgc
     3661 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3721 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3781 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3841 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccagcgccct gatagacggt
     3901 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3961 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4021 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4081 attaacgctt acaattt
//
