LOCUS       Exported               13382 bp ds-DNA     circular SYN 10-DEC-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13382)
  AUTHORS   Venken Lab
  TITLE     Direct Submission
  JOURNAL   Exported Dec 10, 2018 from SnapGene 4.2.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13382
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    26..74
                     /note="synthetic polyadenylation signal"
     misc_feature    88..179
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     protein_bind    192..243
                     /label=RE
                     /bound_moiety="NF-kappa-B"
                     /note="NF-kappa-B response element (5 copies)"
     promoter        290..321
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             355..2001
                     /codon_start=1
                     /label=RedFirefly
                     /translation="MENMENDENIVVGPKPFYPIEEGSAGTQLRKYMERYAKLGAIAFT
                     NAVTGVDYSYAEYLEKSCCLGKALQNYGLVVDGRIALCSENCEEFFIPVIAGLFIGVGV
                     APTNEIYTLRELVHSLGISKPTIVFSSKKGLDKVITVQKTVTTIKTIVILDSKVDYRGY
                     QCLDTFIKRNTPPGFQASSFKTVEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFS
                     HARDPIYGNQVSPGTAVLTVVPLHHGFGMFTTLGYLICGFRVVMLTKFDEETFLKTLQD
                     YKCTYVILVPTLFAILNKSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRFNLPGVRQ
                     GYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPML
                     MKGYVNNPEATKELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELES
                     VLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGG
                     VRFVDEVPKGLTGKIDGRAIREILKKPVAKM"
     polyA_signal    2006..2230
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2255..2303
                     /note="synthetic polyadenylation signal"
     misc_feature    2317..2408
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     misc_binding    2421..2427
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     misc_binding    2432..2439
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     misc_binding    2447..2454
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     misc_binding    2459..2466
                     /allele="SMAD2/SMAD3/SMAD4 "
                     /label=SMAD_BE
                     /bound_moiety="SMAD2/SMAD3/SMAD4 "
     promoter        2519..2550
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             2584..4236
                     /codon_start=1
                     /transl_table=11
                     /gene="luc"
                     /product="Firelfly luciferase Photinus pyralis"
                     /label=Firefly Luciferase
                     /note="firefly luciferase"
                     /db_xref="GI:155733599"
                     /protein_id="ABU39926.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     gene            2584..4236
                     /gene="luc"
                     /label=luc
                     /note="LUC"
     polyA_signal    4241..4465
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    4478..4526
                     /note="synthetic polyadenylation signal"
     misc_feature    4540..4631
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     misc_feature    4644..4681
                     /label=Myc_RE
     promoter        4728..4759
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             4793..5725
                     /codon_start=1
                     /label=Renilla_HS
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    5730..5954
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    5967..6015
                     /note="synthetic polyadenylation signal"
     misc_feature    6029..6120
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     protein_bind    6133..6186
                     /label=RE
                     /bound_moiety="p53"
                     /note="p53 response element created by fusing two human p53
                     binding sites"
     promoter        6233..6264
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             6298..6813
                     /codon_start=1
                     /label=NLuc_HS
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     polyA_signal    6818..7042
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    7055..7103
                     /note="synthetic polyadenylation signal"
     misc_feature    7117..7208
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     misc_binding    7221..7227
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     misc_binding    7229..7235
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     misc_binding    7237..7243
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     misc_binding    7245..7251
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     misc_binding    7253..7259
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     misc_binding    7261..7267
                     /label=AP-1
                     /bound_moiety="AP-1 binding element "
     promoter        7321..7352
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             7386..8321
                     /codon_start=1
                     /label=GreenRenilla_HS
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNATSSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGSALAFHYAYEHQDRIKAIVHMESVVDVIESWMGWPDIEEEL
                     ALIKSEEGEKMVLENNFFVETLLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKLFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFLQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    8327..8551
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        8624..8927
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8928..9131
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             9161..10843
                     /codon_start=1
                     /label=GrLuc
                     /translation="MMKREKNVVYGPEPKHPLGNFTAGEMLYNALHKHSHIPQAILDVM
                     GNESLSYQEFFDTTVKLGQSLQNCGYKMNDVVSICAENNKRFFIPIISAWYIGMVVAPV
                     NEDYIPDELCKVTGISKPILVFTTRKILPKVLEVKDRTNYIKRIIILDSEENLLGCESL
                     HNFMSRYSDNNLQTFKPLHYDPVDQVAAILCSSGTTGLPKGVMQTHRNICVRLTHASDP
                     RVGTQLIPGVSVLAYLPFFHAFGFSINLGYFMVGLRVVMLRRFNQEVFLKAIQDYEVRS
                     VINVPSTILFLSKSPLVDKYDLSTLAELCCGAAPLAKEVAEIAVKRLNLPGIRCGYGLT
                     ESTSANIHTLHNEFKSGSLGKVTPYMAAKIIDRNTGEALGPNQVGELCIWGPMVTKGYV
                     NNPQATKEAIDDDGWLHSGDFGYYDEDEYFYIVDRYKELIKYKGYQVAPVELEEILLQH
                     PGIRDVAVVGIPDIEAGELPAGFVVKQPGAQLTAKEVYDFLAQRVSHSKYLRGGVRFVD
                     SIPRNVTGKISRKELREALMEKAGGGSMYKTSAANDENYALAA"
     polyA_signal    10848..11072
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(11174..11856)
                     /direction=LEFT
                     /label=ColE1 origin
     promoter        11895..11993
                     /label=cat Promoter
     CDS             11998..12810
                     /codon_start=1
                     /label=Kanamycin Resistance
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      12815..12922
     rep_origin      12927..13382
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 taacaagctt cgtctcagtc aggagaataa aatatcttta ttttcattac atctgtgtgt
       61 tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca
      121 aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctctc
      181 gctgtcagga ggggaatttc cggggacttt ccgggaattt ccggggactt tccgggaatt
      241 tcctccctcg aggatatcaa atctggcctc ggcggccaag cttagacact agagggtata
      301 taatggaagc tcgacttcca gcttggcaat ccggtactgt tggtaaagcc accaatggag
      361 aacatggaga acgacgagaa cattgtggtg ggacccaagc cgttctaccc gatcgaggag
      421 ggttccgctg gcacccagct gcgcaagtac atggagcgct acgctaagct gggtgctatc
      481 gccttcacca acgccgtgac cggtgtggat tactcctacg ccgagtacct ggagaagtcc
      541 tgctgcctcg gaaaggccct gcagaactac ggtctggtcg tggatggccg cattgccctc
      601 tgctcggaga actgcgagga gttcttcatt cccgtgatcg ctggtctgtt catcggagtc
      661 ggcgttgccc caaccaacga gatctacacc ctgcgcgagc tggtccacag cctgggtatc
      721 agcaagccga ccatcgtttt ctcgagcaag aagggcctcg acaaggttat caccgtgcaa
      781 aaaaccgtca ccaccatcaa gaccatcgtt atcctggatt ccaaggttga ctaccgcggt
      841 taccagtgcc tcgacacctt catcaagcgt aacaccccac ccggcttcca agcctcctcc
      901 ttcaaaaccg tggaggttga tcgcaaggag caggtggccc tgatcatgaa ctcctccggc
      961 tccaccggtc tgccaaaggg cgtgcagctg acccacgaga acaccgtcac ccgcttctcg
     1021 catgcccgtg atcccatcta cggcaaccag gtgtccccag gcaccgccgt cctgaccgtc
     1081 gtgcccctcc accacggctt cggcatgttc accactctcg gctacttgat ctgcggcttc
     1141 cgtgtggtga tgctgactaa gttcgacgag gagactttcc tcaagaccct gcaagactac
     1201 aagtgcactt acgtgatcct ggtgccgacc ctgttcgcca tcctgaacaa gagcgagctg
     1261 ttgaacaaat acgacctgtc gaacctggtg gagatcgctt ccggcggcgc cccactgtcg
     1321 aaggaagttg gcgaggccgt tgctcgccgc ttcaacctgc ccggcgtccg tcagggttac
     1381 ggcctgaccg agactacctc ggctatcatc atcacccccg agggcgacga taagcccggc
     1441 gcttccggca aggttgttcc actcttcaaa gccaaggtga tcgacctgga caccaagaag
     1501 tccctgggtc ccaaccgtcg cggagaagtg tgcgtcaagg gccccatgct gatgaagggc
     1561 tacgtcaaca acccagaggc caccaaggag ttgatcgacg aggagggctg gctgcacact
     1621 ggtgacatcg gttactacga cgaagagaag cacttcttca tcgttgaccg cctgaagtcc
     1681 ttgatcaagt acaagggcta ccaggttccc cccgccgagc tcgagagcgt cctgctgcag
     1741 cacccctcca ttttcgatgc cggagtggcc ggtgtccccg atcccgtggc tggcgagctg
     1801 ccaggtgccg tcgtcgtgct ggagtccggc aagaacatga ccgagaagga agtgatggat
     1861 tacgtggcct cgcaagtgtc caacgccaag cgcctgcgcg gcggcgtgcg cttcgtggat
     1921 gaggtgccca agggcctcac cggaaagatc gacggtcgcg ctatccgcga gatcctcaag
     1981 aagcccgtgg ccaagatgtg agcttctgtg ccttctagtt gccagccatc tgttgtttgc
     2041 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     2101 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     2161 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     2221 ggctctatgg cgctgtcagg agcgctgtca ggagaataaa atatctttat tttcattaca
     2281 tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa
     2341 aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac
     2401 atttctctcg ctgtcaggag gtctagatgc cgtctagact tagtacgtct agactgccgt
     2461 ctagacggat cctccctcga ggatatcaaa tctggcctcg gcggccaagc ttagacacta
     2521 gagggtatat aatggaagct cgacttccag cttggcaatc cggtactgtt ggtaaagcca
     2581 ccaatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat
     2641 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca
     2701 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg
     2761 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc
     2821 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga
     2881 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc
     2941 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg
     3001 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag
     3061 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac
     3121 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct
     3181 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc
     3241 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt
     3301 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga
     3361 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat
     3421 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact
     3481 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc
     3541 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga
     3601 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg
     3661 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg
     3721 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc
     3781 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat
     3841 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag
     3901 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc
     3961 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa
     4021 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg
     4081 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg
     4141 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc
     4201 ataaaggcca agaagggcgg aaagatcgcc gtgtaagctt ctgtgccttc tagttgccag
     4261 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     4321 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4381 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4441 gctggggatg cggtgggctc tatggcgctg tcaggagaat aaaatatctt tattttcatt
     4501 acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc tccatcaaaa
     4561 caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg caggtgccag
     4621 aacatttctc tcgctgtcag gagcacgtgt gcacgtggac acgtgctcac gtgctcacgt
     4681 gtccctcgag gatatcaaat ctggcctcgg cggccaagct tagacactag agggtatata
     4741 atggaagctc gacttccagc ttggcaatcc ggtactgttg gtaaagccac caatgacttc
     4801 caaggtttat gaccccgagc aacgcaagag aatgataact gggcctcagt ggtgggccag
     4861 atgtaaacaa atgaatgtcc ttgatagttt catcaattac tacgacagtg aaaagcatgc
     4921 agaaaacgcc gtaatttttt tgcatggcaa cgcagcctcc tcttatttgt ggcgacacgt
     4981 agtcccgcat atagagccgg tagctaggtg tattatccct gatctgatcg gaatgggaaa
     5041 gagtggcaag tcagggaatg gatcttatcg ccttcttgac cactacaagt atctgacggc
     5101 ttggttcgaa ctcttgaatt tgccgaagaa aatcatcttc gtgggtcacg attggggagc
     5161 ctgtctcgcg ttccattatt cctacgagca tcaggacaag ataaaggcaa tcgttcacgc
     5221 cgagtctgta gtagatgtaa tcgagtcctg ggacgaatgg ccagatattg aggaggatat
     5281 agcattgatt aaaagcgagg aaggggaaaa gatggtgctg gagaacaact tctttgtgga
     5341 aacaatgctt ccttcaaaaa tcatgagaaa actggaaccc gaggaatttg ccgcttatct
     5401 cgaaccgttt aaagaaaagg gagaagtccg caggccaaca ctttcctggc caagagaaat
     5461 cccgctcgtc aaaggcggaa agccggatgt tgtgcaaata gtccgaaatt acaacgcgta
     5521 cctccgggcc tctgacgatc tccctaaaat gtttatagaa tcagatccag gattttttag
     5581 caacgctatc gtagaaggag caaagaaatt tccaaatacc gaatttgtca aagtaaaggg
     5641 actgcatttc agtcaagagg acgctccaga cgagatgggc aaatacataa agtcctttgt
     5701 agagagggtc ctgaagaacg aacaggcttc tgtgccttct agttgccagc catctgttgt
     5761 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     5821 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     5881 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     5941 ggtgggctct atggcgctgt caggagaata aaatatcttt attttcatta catctgtgtg
     6001 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac
     6061 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
     6121 cgctgtcagg agtacagaac atgtctaagc atgctgtgcc ttgcctggac ttgcctggcc
     6181 ttgccttccc tcgaggatat caaatctggc ctcggcggcc aagcttagac actagagggt
     6241 atataatgga agctcgactt ccagcttggc aatccggtac tgttggtaaa gccaccaatg
     6301 gtgtttacct tggaggactt cgtgggtgac tggaggcaaa ctgcaggata caatttggac
     6361 caggtcctcg aacagggagg agtcagcagc cttttccaaa acttgggcgt gtccgttacc
     6421 cctattcaga gaattgtcct ttccggagag aacggattga aaatagatat ccacgtcata
     6481 atcccgtatg aaggactgtc tggggaccaa atgggtcaga ttgaaaaaat attcaaagta
     6541 gtttatcccg ttgatgatca tcacttcaaa gttatccttc attatgggac tttggtaatc
     6601 gatggagtta ctcctaatat gatagattac tttggccgac cttacgaggg gatcgctgta
     6661 ttcgacggca agaaaatcac cgtcactgga accctctgga acggcaacaa gattattgac
     6721 gagcgattga tcaatccaga cggatctttg cttttccgcg taacgataaa cggcgtaaca
     6781 gggtggaggc tctgcgaacg aatactcgcc tgagcttctg tgccttctag ttgccagcca
     6841 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     6901 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     6961 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     7021 ggggatgcgg tgggctctat ggcgctgtca ggagaataaa atatctttat tttcattaca
     7081 tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa
     7141 aacgaaacaa aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac
     7201 atttctctcg ctgtcaggag tgagtcagtg actcagtgag tcagtgactc agtgagtcag
     7261 tgactcaggc atgctccctc gaggatatca aatctggcct cggcggccaa gcttagacac
     7321 tagagggtat ataatggaag ctcgacttcc agcttggcaa tccggtactg ttggtaaagc
     7381 caccaatggc ttccaaagtc tacgacccag aacaaaggaa gaggatgatt actggaccgc
     7441 agtggtgggc aaggtgcaag cagatgaatg tactcgatag ttttatcaat tattacgact
     7501 ccgaaaaaca cgcggaaaat gctgtcatat tcctgcacgg gaacgccact agcagctact
     7561 tgtggcggca cgtagtcccc cacatagagc ctgttgcccg atgtattatt cccgatctca
     7621 ttggaatggg caaatcaggc aaatctggga acggttctta ccgcctgctc gatcactata
     7681 aatatttgac cgcttggttt gagctgctta atctccctaa aaaaataata ttcgttggac
     7741 acgactgggg ctcagctctg gcgttccact acgcatacga gcatcaagac cgaataaaag
     7801 caatagtaca tatggagtca gttgttgacg tcatagagtc atggatgggt tggccagata
     7861 ttgaagagga attggccctc atcaagagtg aggaggggga aaaaatggtc ctggaaaata
     7921 attttttcgt ggaaaccttg ctcccgagta aaataatgcg aaaacttgaa cccgaagagt
     7981 ttgctgctta tttggagcct ttcaaggaaa agggggaagt aagacgacca accctttctt
     8041 ggccgaggga gatacctctt gttaagggtg gaaagcccga cgtagtccag atagtccgaa
     8101 attacaacgc gtacctgaga gctagtgatg acctccctaa gttgtttatc gagtcagacc
     8161 ctgggttctt cagcaacgca attgtagaag gagcgaaaaa atttccaaat acagaatttg
     8221 tgaaggttaa ggggctccac tttttgcaag aggacgcccc cgatgagatg ggaaaataca
     8281 ttaaatcttt cgtagagcgc gtccttaaga atgaacagtg aggcttctgt gccttctagt
     8341 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     8401 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     8461 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     8521 aggcatgctg gggatgcggt gggctctatg gcgctgtcag gagtagttat taatagtaat
     8581 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     8641 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     8701 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     8761 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     8821 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     8881 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     8941 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     9001 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     9061 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     9121 taagcagagc tggtttagtg aaccgtcaga tcaagctcaa atgatgaaga gagagaagaa
     9181 tgttgtgtat gggcccgagc ctaagcaccc gcttgggaat tttaccgctg gagagatgct
     9241 ctacaacgcg cttcacaaac atagtcacat cccgcaggcg atcctcgacg tgatgggtaa
     9301 cgaaagtctt agttatcagg aattcttcga cacgacggtc aaattggggc agagtctcca
     9361 gaattgtggc tataaaatga atgatgttgt atcaatatgc gctgaaaaca acaaaagatt
     9421 tttcattcca attattagtg cgtggtatat aggcatggtc gttgcacctg taaacgagga
     9481 ctatatacca gacgaactgt gtaaggtcac aggaattagt aagccaatac ttgtgttcac
     9541 cacgcgaaag atacttccaa aggttttgga ggttaaggat agaacaaatt atatcaaaag
     9601 aatcataatt ctggactctg aggaaaacct tctcggatgt gaatccttgc ataattttat
     9661 gtcaagatat agcgataaca atcttcagac ctttaaacca ctgcattacg atccggtcga
     9721 ccaggttgca gcgatactgt gtagtagtgg tactaccggg ttgcctaagg gcgtcatgca
     9781 gacccaccgc aatatatgtg ttcggctgac ccacgcctct gacccgagag ttggtaccca
     9841 actcattccc ggagttagtg tgctggcgta tcttcccttc tttcacgcct ttgggttttc
     9901 cattaacttg gggtatttta tggtggggct gcgcgtcgtg atgcttcgga ggttcaacca
     9961 agaggttttt ttgaaggcga ttcaagacta tgaagtacga agtgtgatca atgtcccaag
    10021 tacaatcctc tttctttcta aatcaccgtt ggttgataag tatgatcttt ctaccttggc
    10081 ggaactttgt tgtggtgcgg cacctcttgc aaaagaagta gctgaaatcg ccgtaaaacg
    10141 ccttaatttg ccaggcatac ggtgtggtta cgggttgact gaaagcacat ccgctaatat
    10201 ccacactctt cacaacgaat ttaaatccgg ttcattggga aaagtgacgc cctacatggc
    10261 cgctaaaatt atagatcgaa acaccggaga ggcgcttggc ccaaatcaag tgggagagtt
    10321 gtgcatttgg ggccccatgg tcacgaaagg ctacgtgaat aatccacagg ccaccaagga
    10381 ggccattgac gacgatggtt ggcttcacag tggtgatttt ggctactatg atgaggatga
    10441 atatttttac attgtggata gatataaaga gctgatcaag tacaagggat atcaagtggc
    10501 tcctgtcgag ttggaggaga ttttgctcca acatccaggg atccgcgatg tggccgtagt
    10561 cggtatcccc gacatagaag caggggaact tccggccgga ttcgttgtca agcaacctgg
    10621 ggcccaactg acggctaaag aagtatatga tttccttgca caaagagtta gccacagcaa
    10681 gtatcttcga gggggtgtca gattcgttga ctctattccg aggaacgtca cgggcaaaat
    10741 cagccgaaag gaactccgag aagcacttat ggagaaggcg ggtggtggat ctatgtacaa
    10801 gacatccgcc gccaacgatg agaactatgc cctggcggct tgagcttctg tgccttctag
    10861 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
    10921 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
    10981 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
    11041 caggcatgct ggggatgcgg tgggctctat ggcgcttgag acgaagcttg acacggttat
    11101 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    11161 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    11221 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    11281 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    11341 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    11401 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    11461 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    11521 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    11581 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    11641 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    11701 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    11761 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    11821 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    11881 agatcctttt gtgatgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga
    11941 tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg
    12001 agccatattc aacgggaaac gtcttgctca aggccgcgat taaattccaa catggatgct
    12061 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctac
    12121 cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
    12181 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
    12241 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
    12301 ccagggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
    12361 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
    12421 aacagcgatc gcgtatttcg actcgctcag gcgcaatcac gaatgaataa cggtttggtt
    12481 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
    12541 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
    12601 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
    12661 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
    12721 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
    12781 cagtttcatt tgatgctcga tgagtttttc tgattttttt taaggcagtt attggtgccc
    12841 ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg gcagaaattc
    12901 gatgataagc tgtcaaacat gatgttacgc gccctgtagc ggcgcattaa gcgcggcggg
    12961 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
    13021 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
    13081 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    13141 ttagggtgat ggttcacgta gtgggccagc gccctgatag acggtttttc gccctttgac
    13201 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    13261 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
    13321 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
    13381 tt
//
