LOCUS       pT2-Ip2GI-DestRs        7423 bp    DNA     circular     16-OCT-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
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COMMENT     VNTDATE|587219883|
COMMENT     VNTDBDATE|711983245|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pT2-Ip2GI-DestRsm-cytobow-4xnrUAS|
COMMENT     VNTAUTHORNAME|Albert Pan|
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COMMENT                                   0 -10 9.06643 1.17389 #247#) 
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COMMENT                                   "near consensus UAS" "@N" 1 22.7068 1.74233 0 
COMMENT                                   -10 8.66681 1.17389 #249#) 
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FEATURES             Location/Qualifiers
     misc_feature    complement(6439..7299)
                     /vntifkey="21"
                     /label=Amp\(R)
     misc_feature    complement(5278..5294)
                     /vntifkey="21"
                     /label=M13R
     misc_feature    complement(4636..4970)
                     /vntifkey="21"
                     /label=Tol2\exon1
     misc_feature    complement(706..1052)
                     /vntifkey="21"
                     /label=Tol2\exon4
     misc_feature    complement(5134..5152)
                     /vntifkey="21"
                     /label=Tol2\5'\TIR
     misc_feature    complement(517..533)
                     /vntifkey="21"
                     /label=Tol2\3'\TIR
     misc_binding    1079..1096
                     /vntifkey="20"
                     /label=I-SceI
     misc_binding    4607..4624
                     /vntifkey="20"
                     /label=I-SceI
     misc_feature    2600..3319
                     /vntifkey="21"
                     /label=Cerulean
     misc_feature    1418..1451
                     /vntifkey="21"
                     /label=Lox2272
     misc_feature    2547..2580
                     /vntifkey="21"
                     /label=Lox2272
     misc_feature    3570..3603
                     /vntifkey="21"
                     /label=LoxP
     misc_feature    1460..1493
                     /vntifkey="21"
                     /label=LoxP
     exon            1512..2216
                     /vntifkey="61"
                     /label=dTomato
     exon            3617..4336
                     /vntifkey="61"
                     /label=EYFP
     primer          1485..1504
                     /vntifkey="27"
                     /label=dTom-f1
     primer          2519..2537
                     /vntifkey="27"
                     /label=dtom-r1
     misc_feature    1125..1148
                     /vntifkey="21"
                     /label=AttB1
     promoter        1322..1332
                     /ApEinfo_fwdcolor=#ffff7f
                     /ApEinfo_revcolor=#ffff7f
                     /vntifkey="30"
                     /label=adenovirus\E1B\TATA
     5'UTR           1343..1374
                     /ApEinfo_fwdcolor=#ffff3f
                     /ApEinfo_revcolor=#ffff3f
                     /vntifkey="52"
                     /label=carp\beta-actin\trx\start
     promoter        1281..1297
                     /ApEinfo_fwdcolor=#ffddbd
                     /ApEinfo_revcolor=#ffddbd
                     /vntifkey="30"
                     /label=near\consensus\UAS
     promoter        1254..1270
                     /ApEinfo_fwdcolor=#ffddbd
                     /ApEinfo_revcolor=#ffddbd
                     /vntifkey="30"
                     /label=synthetic\near\consensus\UAS
     promoter        1227..1243
                     /ApEinfo_fwdcolor=#ffddbd
                     /ApEinfo_revcolor=#ffddbd
                     /vntifkey="30"
                     /label=Gal10(2)\UAS
     promoter        1200..1216
                     /ApEinfo_label=Gal10(2) UAS
                     /ApEinfo_fwdcolor=#ffddbd
                     /ApEinfo_revcolor=#ffddbd
                     /vntifkey="30"
                     /label=Gal10(2)\UAS(1)
     misc_feature    1274..1274
                     /vntifkey="21"
                     /label=A-G\change\from\Halpern\sequence
     misc_feature    1200..1374
                     /vntifkey="21"
                     /label=4xnrUAS
     misc_feature    1322..1380
                     /vntifkey="21"
                     /label=E1B\promoter
     misc_feature    2236..2464
                     /vntifkey="21"
                     /label=sv40
     misc_feature    3335..3563
                     /vntifkey="21"
                     /label=sv40
     misc_feature    4352..4580
                     /vntifkey="21"
                     /label=sv40
     misc_feature    1382..1405
                     /vntifkey="21"
                     /label=AttB2
BASE COUNT     2011 a      1922 c      1747 g      1743 t 
ORIGIN
        1 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat 
       61 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata 
      121 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt 
      181 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac gctggctaag 
      241 aactcatcag cctccccggt ccatctaccc acgtaccaat gcaccaattg gccacaatga 
      301 cggctactac atggtgccat tccttcctct ttataggaat ggagactacc tcctgtccaa 
      361 caatgctctt ggatacgagt acgcctacct gttggaccca ggtcattgca caacaccaga 
      421 aatgccctct gatctgcaaa agacgtgaat aatctgttca gacacccata tgcactctgt 
      481 tccacacagg tcagaggttt gtccaggagt tcttgacaga ggtgtaaaaa gtactcaaaa 
      541 attttactca agtgaaagta caagtactta gggaaaattt tactcaatta aaagtaaaag 
      601 tatctggcta gaatcttact tgagtaaaag taaaaaagta ctccattaaa attgtacttg 
      661 agtattaagg aagtaaaagt aaaagcaaga aagaaaacta gagattcttg tttaagcttt 
      721 taatctcaaa aaacattaaa tgaaatgcat acaaggtttt atcctgcttt agaactgttt 
      781 gtatttaatt atcaaactat aagacagaca atctaatgcc agtacacgct actcaaagtt 
      841 gtaaaacctc agatttaact tcagtagaag ctgattctca aaattgttag tgtcaagcct 
      901 agctcttttg gggctgaaaa gcaatcctgc agtgctgaaa agcctctcac aggcagccga 
      961 tgcgggaaga ggtgtattag tcttgataga gaggctgcaa atagcaggaa acgtgagcag 
     1021 agactccctg gtgtctgaaa cacaggccag atgggccatt cgtacgctta atttcgtata 
     1081 gggataacag ggtaatgata tcgatatcaa gggcccatct cgagcaagtt tgtacaaaaa 
     1141 agcaggctcc gaattcctgc agcccggggg atcacgcggc catcaagctt aggcctccac 
     1201 ggtggcttct aatccgtgag tcctagcggg tgacagccct ccgtcttcac aggcggagga 
     1261 gagtcttccg tagggttcct cggagtactg tcctccgacg cgtgcaaggg tcgactctag 
     1321 agggtatata atggatccca tcgcgtctca gcctcacttt gagctcctcc acacgaattc 
     1381 gacccagctt tcttgtacaa agtgggctag cgagctcata acttcgtata ggatacttta 
     1441 tacgaagtta taactcgaga taacttcgta tagcatacat tatacgaagt tataccggca 
     1501 gatctgccac catggtgagc aagggcgagg aggtcatcaa agagttcatg cgcttcaagg 
     1561 tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc 
     1621 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct 
     1681 tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc 
     1741 ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg 
     1801 tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg 
     1861 gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa 
     1921 tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg 
     1981 tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg 
     2041 agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg 
     2101 acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc 
     2161 gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac aagtaaagtg 
     2221 tgcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt 
     2281 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg 
     2341 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 
     2401 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 
     2461 cttaaggcgt aaattgtaag cgttaatgct ccgagggccg ccaccacctg ttcctgtagg 
     2521 aattggccgc gactctagga tctaggataa cttcgtatag gatactttat acgaagttat 
     2581 tgatccaccg gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 
     2641 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 
     2701 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 
     2761 gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcgcccg 
     2821 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt 
     2881 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 
     2941 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 
     3001 cggcaacatc ctggggcaca agctggagta caacgccatc agcgacaacg tctatatcac 
     3061 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga 
     3121 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 
     3181 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga 
     3241 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 
     3301 ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt 
     3361 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa 
     3421 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc 
     3481 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 
     3541 tccaaactca tcaatgtatc ttaatcgaga taacttcgta tagcatacat tatacgaagt 
     3601 tataccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat 
     3661 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga 
     3721 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc 
     3781 cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcagtgct tcgcccgcta 
     3841 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca 
     3901 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt 
     3961 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg 
     4021 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc 
     4081 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg 
     4141 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct 
     4201 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa 
     4261 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga 
     4321 cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta 
     4381 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg 
     4441 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat 
     4501 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 
     4561 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatccttagg gataacaggg 
     4621 taatacgcgt gatctgcgaa gatacggcca cgggtgctct tgatcctgtg gctgattttg 
     4681 gactgtgctg ctcgcagctg ctgatgaatc acatacttcc tccattttct tccactgatt 
     4741 gactgttata atttccctaa tttccaggtc aaggtgctgt gcattgtggt aatagatgtg 
     4801 acatgacgtc acttccaaag gaccaatgaa catgtctgac caatttcata taatgtgaaa 
     4861 acgattttca taggcagaat aaataacatt taaattaaac tgggcatcag cgcaattcaa 
     4921 ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc tccaaaaaat aagtactttt 
     4981 tgactgtaaa taaaattgta aggagtaaaa agtacttttt tttctaaaaa aatgtaatta 
     5041 agtaaaagta aaagtattga tttttaattg tactcaagta aagtaaaaat ccccaaaaat 
     5101 aatacttaag tacagtaatc aagtaaaatt actcaagtac tttacacctc tggttcttga 
     5161 ccccctacct tcagcaagcc cagcagatcc actagttcta gagcggccgc caccgcggtg 
     5221 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc 
     5281 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 
     5341 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 
     5401 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 
     5461 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 
     5521 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 
     5581 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 
     5641 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 
     5701 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 
     5761 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 
     5821 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 
     5881 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 
     5941 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 
     6001 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 
     6061 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 
     6121 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 
     6181 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 
     6241 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 
     6301 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 
     6361 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 
     6421 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 
     6481 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 
     6541 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 
     6601 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 
     6661 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 
     6721 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 
     6781 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 
     6841 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 
     6901 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 
     6961 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 
     7021 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 
     7081 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 
     7141 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 
     7201 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 
     7261 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 
     7321 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 
     7381 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtg 
//
