LOCUS       pBlu2SKP-I-sceI-        6768 bp    DNA     circular      6-APR-2017
DEFINITION  pBluescript II SK(+) vector DNA, phagemid excised from lambda ZAPII.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11707927|
COMMENT     VNTDBDATE|-11707927|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBlu2SKP-I-sceI-14xUAS-cytobow (Zebrabow-V)|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBlu2SKP-I-sceI-14xUAS-cytobow (Zebrabow-V)" 0 6768 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 3452816845 3452816845 3452816845 
COMMENT        3452816845 (CObList) (CObList) (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 1 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6768 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 2 5275 0 6521 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 4 699 0 1296 0 1618 0 4491 
COMMENT            0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 1135 0 4497 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 715 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 1177 0 4455 0 4479 0 0 
COMMENT            "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 720 0 4467 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 1352 0 1775 0 2440 0 3457 
COMMENT            0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 4 1697 0 3667 0 4453 0 4489 
COMMENT            0 0 "") #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 4493 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 4491 0 0 "")) 
COMMENT       (CObList 
COMMENT        #10=(CFSignal (CObList) "T7 primer binding site" 27 0 0 655 676 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #11=(CFSignal (CObList) "M13 R" 21 0 0 4619 4635 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #12=(CFSignal (CObList) "M13 F" 21 0 0 600 615 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #13=(CFSignal (CObList) "T7 promoter" 29 0 0 656 672 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "T3 promoter" 29 0 1 4549 4568 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "I-sceI" 21 0 0 619 648 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "I-sceI" 21 0 0 4576 4605 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #17=(CFSignal (CObList) "E1B forward primer" 27 0 0 1141 1164 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "Frank-insert-forward" 27 0 0 1176 1194 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "UAS-A" 21 0 0 1060 1105 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #20=(CFSignal (CObList) "UAS-A" 21 0 0 732 777 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #21=(CFSignal (CObList) "UAS-B" 21 0 0 1006 1059 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #22=(CFSignal (CObList) "UAS-B" 21 0 0 778 831 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #23=(CFSignal (CObList) "UAS-B" 21 0 0 877 930 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #24=(CFSignal (CObList) "2xUAS" 21 0 0 832 877 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #25=(CFSignal (CObList) "2xUAS" 21 0 0 931 1005 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #26=(CFSignal (CObList) "E1b" 21 0 0 1123 1158 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #27=(CFSignal (CObList) "Cerulean" 21 0 0 2441 3160 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #28=(CFSignal (CObList) "Lox2272" 21 0 0 1259 1292 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #29=(CFSignal (CObList) "Lox2272" 21 0 0 2388 2421 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #30=(CFSignal (CObList) "LoxP" 21 0 0 3411 3444 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #31=(CFSignal (CObList) "LoxP" 21 0 0 1301 1334 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #32=(CFSignal (CObList) "dTomato" 61 0 0 1353 2057 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #33=(CFSignal (CObList) "EYFP" 61 0 0 3458 4177 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #34=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #35=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) 
COMMENT                    "pBlu2SKP-I-sceI-14xUAS-cytobow (Zebrabow-V)" 2) 5 "" 0 4) 
COMMENT              #36=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pBlu2SKP-I-sceI-14xUAS-cytobow (Zebrabow-V)'" 
COMMENT                          1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "pBluescript II SK(+) vector DNA, phagemid excised from lambda ZAPII" 
COMMENT                          1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: Albert Pan" 
COMMENT                          1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 04/06/17 11:22AM" 1) 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 04/06/17 11:22AM" 1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6768 bp" 1) 
COMMENT                     #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #45=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) (CObjectList)) 
COMMENT                   "Standard Fields") 
COMMENT              #46=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Albert Pan" 
COMMENT                          1))) "Original Author") 
COMMENT              #48=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #49=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
COMMENT              #50=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #51=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #52=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 600 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 600   End: 615 " 1))) "M13 F") 
COMMENT                            #54=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 619 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 619   End: 648 " 1))) "I-sceI") 
COMMENT                            #56=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 732 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 732   End: 777 " 1))) "UAS-A") 
COMMENT                            #58=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 778 (3 #22# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 778   End: 831 " 1))) "UAS-B") 
COMMENT                            #60=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 832 (3 #24# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 832   End: 877 " 1))) "2xUAS") 
COMMENT                            #62=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 877 (3 #23# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 877   End: 930 " 1))) "UAS-B") 
COMMENT                            #64=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 931 (3 #25# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 931   End: 1005" 1))) "2xUAS") 
COMMENT                            #66=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1006 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1006  End: 1059" 1))) "UAS-B") 
COMMENT                            #68=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1060 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1060  End: 1105" 1))) "UAS-A") 
COMMENT                            #70=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1123 (3 #26# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1123  End: 1158" 1))) "E1b") 
COMMENT                            #72=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1259 (3 #28# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1259  End: 1292" 1))) "Lox2272") 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1301 (3 #31# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1301  End: 1334" 1))) "LoxP") 
COMMENT                            #76=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2388 (3 #29# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2388  End: 2421" 1))) "Lox2272") 
COMMENT                            #78=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2441 (3 #27# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2441  End: 3160" 1))) "Cerulean") 
COMMENT                            #80=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3411 (3 #30# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3411  End: 3444" 1))) "LoxP") 
COMMENT                            #82=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4576 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4576  End: 4605" 1))) "I-sceI") 
COMMENT                            #84=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4619 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4619  End: 4635" 1))) "M13 R"))) 
COMMENT                          "Misc. Feature (17 total)") 
COMMENT                     #86=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 27 (7 27 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #87=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 655 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 655   End: 676 " 1))) 
COMMENT                                 "T7 primer binding site") 
COMMENT                            #89=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1141 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1141  End: 1164" 1))) 
COMMENT                                 "E1B forward primer") 
COMMENT                            #91=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1176 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1176  End: 1194" 1))) 
COMMENT                                 "Frank-insert-forward"))) "Primer (3 total)") 
COMMENT                     #93=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #94=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 656 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 656   End: 672 " 1))) 
COMMENT                                 "T7 promoter") 
COMMENT                            #96=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4549 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4549  End: 4568 (Complementary)" 
COMMENT                                        1))) "T3 promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #98=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 61 (7 61 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #99=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1353 (3 #32# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Start: 1353  End: 2057" 1))) "dTomato") 
COMMENT                            #101=(CFolderPar 
COMMENT                                  (CGroupPar 
COMMENT                                   (CParagraph 3458 (3 #33# 0) 1 2 2 0 194) 
COMMENT                                   (CObjectList 
COMMENT                                    #102=(CLinePar 
COMMENT                                          (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                          "Start: 3458  End: 4177" 1))) "EYFP"))) 
COMMENT                          "Exon (2 total)"))) "Feature Map") 
COMMENT              #103=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #104=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 97952416 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #105=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #106=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 5275 " 1) 
COMMENT                                      #107=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 6521 " 1))))) "ApaLI: 2 sites") 
COMMENT                            1 5 "GTGCAC" "CACGTG") 
COMMENT                      #108=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 97952496 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #109=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #110=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 699 " 1) 
COMMENT                                      #111=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1296 " 1) 
COMMENT                                      #112=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 1618 " 1) 
COMMENT                                      #113=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 4491 " 1))))) "AvaI: 4 sites") 
COMMENT                            1 5 "CYCGRG" "GRGCYC") 
COMMENT                      #114=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 97952576 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #115=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #116=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1135 " 1) 
COMMENT                                      #117=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 4497 " 1))))) "BamHI: 2 sites") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
COMMENT                      #118=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 97952656 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #119=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #120=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 715 " 1))))) "ClaI: 1 site") 2 
COMMENT                            4 "ATCGAT" "TAGCTA") 
COMMENT                      #121=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 97952736 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #122=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #123=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1177 " 1) 
COMMENT                                      #124=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 4455 " 1) 
COMMENT                                      #125=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 4479 " 1))))) "EcoRI: 3 sites") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
COMMENT                      #126=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 97987760 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #127=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #128=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 720 " 1) 
COMMENT                                      #129=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 4467 " 1))))) 
COMMENT                             "HindIII: 2 sites") 1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #130=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 97951432 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #131=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #132=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1352 " 1) 
COMMENT                                      #133=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1775 " 1) 
COMMENT                                      #134=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 2440 " 1) 
COMMENT                                      #135=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #6# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 3457 " 1))))) "NcoI: 4 sites") 
COMMENT                            1 5 "CCATGG" "GGTACC") 
COMMENT                      #136=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 98191416 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #137=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #7# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #138=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1697 " 1) 
COMMENT                                      #139=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3667 " 1) 
COMMENT                                      #140=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 4453 " 1) 
COMMENT                                      #141=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #7# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 4489 " 1))))) "PstI: 4 sites") 
COMMENT                            5 1 "CTGCAG" "GACGTC") 
COMMENT                      #142=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 98224600 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #143=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #8# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #144=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4493 " 1))))) "SmaI: 1 site") 
COMMENT                            3 3 "CCCGGG" "GGGCCC") 
COMMENT                      #145=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 98256824 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #146=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #9# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #147=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #9# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4491 " 1))))) "XmaI: 1 site") 
COMMENT                            1 5 "CCCGGG" "GGGCCC"))) 
COMMENT                    "Restriction/Methylation Map") 
COMMENT              #148=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 20 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #149=(CRefLinePar 
COMMENT                            (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) 
COMMENT                             " 3747: pBlu2SKP-I-sceI: HindIII(720) - HindIII(4467)" 
COMMENT                             1) 4 "" 720 4466) 
COMMENT                      #150=(CRefLinePar 
COMMENT                            (CLinePar (CParagraph 0 (0 0) 0 2 1 0 235) 
COMMENT                             " 3021: pBlu2SKP-I-sceI: HindIII(4467) - HindIII(720)" 
COMMENT                             1) 4 "" 4467 719))) "Restriction Fragments")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #151=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #152=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #153=(CWidgetStyle "Molecule Label 2" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #154=(CWidgetStyle "Molecule Label 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #155=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 
COMMENT                (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #156=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 
COMMENT                (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #157=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 
COMMENT                (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) 
COMMENT          #158=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #159=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #160=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 
COMMENT                (LOGSHAPE 1 1.9 0 0 0)) 
COMMENT          #161=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #162=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #163=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #164=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 
COMMENT                1 65535 "@N (@H)" 0) 
COMMENT          #165=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #166=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #167=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #168=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #169=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) 
COMMENT          #170=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 
COMMENT                (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) 
COMMENT          #171=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #172=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #173=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #174=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #175=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #176=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #177=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #178=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #179=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #180=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #181=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #182=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #183=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #184=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0) 
COMMENT          #185=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #186=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777113 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
COMMENT                (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #187=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #188=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #189=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #190=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 
COMMENT         (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 
COMMENT          (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 -365) 
COMMENT        1 1 0 1 1 
COMMENT        (mapper: 20.6309 -24.9918 40.0378 40.0378 0.01 10 -1 6768 6768 1 0 0) 
COMMENT        #191=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 1953784163 100) 
COMMENT              (CObjectList 
COMMENT               #192=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1921 100) 
COMMENT                     (CObjectList 
COMMENT                      #193=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #157# 97403868 0) 
COMMENT                             (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 
COMMENT                             6.27511 6.27311 1 0.0214037) 0.0926889) 
COMMENT                      #194=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #154# 82 100) 
COMMENT                            (LOGPEN 0 0 13408563) 1 
COMMENT                            (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 
COMMENT                            1.74233 0.833333 0 
COMMENT                            "pBlu2SKP-I-sceI-14xUAS-cytobow (Zebrabow-V)" "@N" 1 
COMMENT                            0 0.871165 0 -10 26.9495 1.44863 Nil) 
COMMENT                      #195=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #153# 47407336 100) 
COMMENT                            (LOGPEN 0 0 13408563) 1 
COMMENT                            (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 
COMMENT                            1.74233 0.555556 0 "6768 bp" "@L bp" 16 0 -0.871165 
COMMENT                            0 -10.8 2.89726 0.974079 Nil)) (CObjectList)) 
COMMENT               #196=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1642 100) 
COMMENT                     (CObjectList 
COMMENT                      #197=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -1712 100) 
COMMENT                            (CObjectList 
COMMENT                             #198=(CScratch 
COMMENT                                   (CWidget 0 (3 #11# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 1.99281 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #199=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 97485968 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "M13 R" "@N" 1 
COMMENT                                   -13.9007 6.96932 0 -10 2.59755 1.17389 #198#) 
COMMENT                             #200=(CScratch 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.71798 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #201=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 97600784 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "M13 F" "@N" 1 
COMMENT                                   9.65256 22.6503 0 -10 2.52262 1.17389 #200#) 
COMMENT                             #202=(CScratch 
COMMENT                                   (CWidget 0 (3 #15# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.70037 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #203=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 252 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "I-sceI" "@N" 1 
COMMENT                                   9.69285 20.908 0 -10 2.3228 1.17389 #202#) 
COMMENT                             #204=(CScratch 
COMMENT                                   (CWidget 0 (3 #16# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 2.03267 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #205=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 1000 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "I-sceI" "@N" 1 
COMMENT                                   -13.7625 5.22699 0 -10 2.3228 1.17389 #204#) 
COMMENT                             #206=(CScratch 
COMMENT                                   (CWidget 0 (3 #19# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.29161 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #207=(CLabel (CWidget 0 (0 0) 1 2 0 0 #152# 94 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "UAS-A" "@N" 1 
COMMENT                                   14.8601 0 0 -10 2.7474 1.17389 #206#) 
COMMENT                             #208=(CScratch 
COMMENT                                   (CWidget 0 (3 #20# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.59563 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #209=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 59103368 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "UAS-A" "@N" 1 
COMMENT                                   10.4871 10.454 0 -10 2.7474 1.17389 #208#) 
COMMENT                             #210=(CScratch 
COMMENT                                   (CWidget 0 (3 #21# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.34166 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #211=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 59015616 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "UAS-B" "@N" 1 
COMMENT                                   14.7668 1.74233 0 -10 2.7474 1.17389 #210#) 
COMMENT                             #212=(CScratch 
COMMENT                                   (CWidget 0 (3 #22# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.55299 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #213=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 59609216 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "UAS-B" "@N" 1 
COMMENT                                   12.0707 8.71165 0 -10 2.7474 1.17389 #212#) 
COMMENT                             #214=(CScratch 
COMMENT                                   (CWidget 0 (3 #23# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.46123 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #215=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 47383548 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "UAS-B" "@N" 1 
COMMENT                                   13.9716 5.22699 0 -10 2.7474 1.17389 #214#) 
COMMENT                             #216=(CScratch 
COMMENT                                   (CWidget 0 (3 #24# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.50294 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #217=(CLabel (CWidget 0 (0 0) 1 2 0 0 #152# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "2xUAS" "@N" 1 
COMMENT                                   13.232 6.96932 0 -10 2.82233 1.17389 #216#) 
COMMENT                             #218=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #25# 0) 1 2 0 0 #156# 1166 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 0.835356 5.34166 
COMMENT                                    5.41118 1 0.082322) 0.8 1.8 0) 
COMMENT                             #219=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 97485072 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "2xUAS" "@N" 1 
COMMENT                                   14.5138 3.48466 0 -10 2.82233 1.17389 #218#) 
COMMENT                             #220=(CScratch 
COMMENT                                   (CWidget 0 (3 #26# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.23322 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #221=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 47408684 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "E1b" "@N" 1 
COMMENT                                   14.2179 -1.74233 0 -10 1.67342 1.17389 #220#) 
COMMENT                             #222=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #27# 0) 1 2 0 0 #156# 0 100) 
COMMENT                                    (LOGPEN 0 0 6723840) 1 
COMMENT                                    (LOGBRUSH 0 10079334 0) 1 3.34422 4.01158 1 
COMMENT                                    0.082322) 0.8 1.8 0) 
COMMENT                             #223=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 937 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Cerulean" "@N" 
COMMENT                                   1 10.02 -27.8773 0 -10 3.74646 1.17389 #222#) 
COMMENT                             #224=(CScratch 
COMMENT                                   (CWidget 0 (3 #28# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.10716 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #225=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 783511093 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Lox2272" "@N" 1 
COMMENT                                   14.8043 -10.454 0 -10 3.54665 1.17389 #224#) 
COMMENT                             #226=(CScratch 
COMMENT                                   (CWidget 0 (3 #29# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 4.0607 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #227=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 47383559 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "Lox2272" "@N" 1 
COMMENT                                   13.6928 -24.3926 0 -10 3.54665 1.17389 #226#) 
COMMENT                             #228=(CScratch 
COMMENT                                   (CWidget 0 (3 #30# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 3.11249 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #229=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 47403308 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "LoxP" "@N" 1 
COMMENT                                   -2.55747 -19.1656 0 -10 2.19792 1.17389 #228#) 
COMMENT                             #230=(CScratch 
COMMENT                                   (CWidget 0 (3 #31# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.06823 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #231=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 3080202 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "LoxP" "@N" 1 
COMMENT                                   14.5278 -13.9386 0 -10 2.19792 1.17389 #230#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #232=(CGroupWidget 
COMMENT                            (CWidget 27 (7 27 0) 1 2 0 0 Nil -1084 100) 
COMMENT                            (CObjectList 
COMMENT                             #233=(CScratch 
COMMENT                                   (CWidget 0 (3 #10# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.667 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #234=(CLabel (CWidget 0 (0 0) 1 2 0 0 #152# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "T7 primer binding site" "@N" 1 13.5761 
COMMENT                                   19.1656 0 -10 9.01648 1.17389 #233#) 
COMMENT                             #235=(CScratch 
COMMENT                                   (CWidget 0 (3 #17# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.21653 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #236=(CLabel (CWidget 0 (0 0) 1 2 0 0 #152# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "E1B forward primer" "@N" 1 16.768 -5.22699 0 
COMMENT                                   -10 7.94249 1.17389 #235#) 
COMMENT                             #237=(CScratch 
COMMENT                                   (CWidget 0 (3 #18# 0) 1 2 0 0 #160# 0 100) 
COMMENT                                   (LOGPEN 0 8 13395507) 10 1 5.18409 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #238=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 783509993 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Frank-insert-forward" "@N" 1 16.748 -6.96932 
COMMENT                                   0 -10 8.41705 1.17389 #237#)) (CObjectList)) 
COMMENT                      #239=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -1502 100) 
COMMENT                            (CObjectList 
COMMENT                             #240=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #174# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 1.16464 5.57881 5.66607) 
COMMENT                                   0.4 0.082322 1.08464 1.8 1.8 0) 
COMMENT                             #241=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 59606752 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 promoter" 
COMMENT                                   "@N" 1 11.8422 15.681 0 -10 5.04523 1.17389 
COMMENT                                   #240#) 
COMMENT                             #242=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #174# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 0.835356 2.05769 2.14496) 
COMMENT                                   0.4 0.082322 0.915356 1.8 1.8 1) 
COMMENT                             #243=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 47408896 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T3 promoter" 
COMMENT                                   "@N" 1 -15.7683 3.48466 0 -10 5.04523 1.17389 
COMMENT                                   #242#)) (CObjectList)) 
COMMENT                      #244=(CGroupWidget 
COMMENT                            (CWidget 61 (7 61 0) 1 2 0 0 Nil -317 100) 
COMMENT                            (CObjectList 
COMMENT                             #245=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #32# 0) 1 2 0 0 #155# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 0.835356 4.36657 
COMMENT                                    5.02003 1 0.082322) 0.8 1.8 0) 
COMMENT                             #246=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 47406668 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "dTomato" "@N" 1 
COMMENT                                   16.5078 -20.908 0 -10 3.67153 1.17389 #245#) 
COMMENT                             #247=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #33# 0) 1 2 0 0 #155# 0 100) 
COMMENT                                    (LOGPEN 0 0 3355545) 1 
COMMENT                                    (LOGBRUSH 0 6724095 0) 0.835356 2.40157 
COMMENT                                    3.06893 1 0.082322) 0.8 1.8 0) 
COMMENT                             #248=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #152# 47406916 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "EYFP" "@N" 1 
COMMENT                                   -7.26731 -13.9386 0 -10 2.3228 1.17389 #247#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #249=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -1293 100) 
COMMENT                     (CObjectList 
COMMENT                      #250=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -526 100) 
COMMENT                            (CObjectList 
COMMENT                             #251=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 5.61139 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #252=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #161# 1791863008 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Cla}I (715)" "@N (@S)" 5 11.1778 
COMMENT                                   13.9386 0 -10 3.67153 0.974079 #251#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #253=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #8# 0) 1 2 0 0 Nil -1154 100) 
COMMENT                            (CObjectList 
COMMENT                             #254=(CScratch 
COMMENT                                   (CWidget 1 (1 #8# 1) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.1096 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #255=(CLabel (CWidget 0 (0 0) 1 2 0 0 #161# 0 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Sma}I (4493)" "@N (@S)" 5 -15.9229 0 0 
COMMENT                                   -10 4.62063 0.974079 #254#)) (CObjectList)) 
COMMENT                      #256=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #9# 0) 1 2 0 0 Nil -1014 100) 
COMMENT                            (CObjectList 
COMMENT                             #257=(CScratch 
COMMENT                                   (CWidget 1 (1 #9# 1) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.11145 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #258=(CLabel (CWidget 0 (0 0) 1 2 0 0 #161# 0 100) 
COMMENT                                   (LOGPEN 0 0 153) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 128 
COMMENT                                   "{\\i Xma}I (4491)" "@N (@S)" 5 -15.5679 
COMMENT                                   -3.48466 0 -10 4.67059 0.974079 #257#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #259=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #0# 0) 1 2 0 0 Nil -1433 100) 
COMMENT                            (CObjectList 
COMMENT                             #260=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 1.38477 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #261=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #151# 47403084 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Apa}LI (5275)" "@N (@S)" 5 -16.3542 
COMMENT                                   8.71165 0 -10 4.8704 0.974079 #260#) 
COMMENT                             #262=(CScratch 
COMMENT                                   (CWidget 2 (1 #0# 2) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 0.229868 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #263=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #151# 59607456 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Apa}LI (6521)" "@N (@S)" 5 -6.51288 
COMMENT                                   13.9386 0 -10 4.84542 0.974079 #262#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #264=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #2# 0) 1 2 0 0 Nil -1363 100) 
COMMENT                            (CObjectList 
COMMENT                             #265=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 5.22209 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #266=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #151# 47382972 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Bam}HI (1135)" "@N (@S)" 5 15.8205 
COMMENT                                   -3.48466 0 -10 5.22007 0.974079 #265#) 
COMMENT                             #267=(CScratch 
COMMENT                                   (CWidget 2 (1 #2# 2) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.10589 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #268=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #151# 97470256 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Bam}HI (4497)" "@N (@S)" 5 -16.2042 
COMMENT                                   1.74233 0 -10 5.34495 0.974079 #267#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #269=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #5# 0) 1 2 0 0 Nil -1154 100) 
COMMENT                            (CObjectList 
COMMENT                             #270=(CScratch 
COMMENT                                   (CWidget 1 (1 #5# 1) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 5.60675 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #271=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #151# 58268208 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Hin}dIII (720)" "@N (@S)" 5 11.841 
COMMENT                                   12.1963 0 -10 5.04523 0.974079 #270#) 
COMMENT                             #272=(CScratch 
COMMENT                                   (CWidget 2 (1 #5# 2) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.1337 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #273=(CLabel (CWidget 0 (0 0) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Hin}dIII (4467)" "@N (@S)" 5 -14.7539 
COMMENT                                   -8.71165 0 -10 5.44485 0.974079 #272#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #274=(CGroupWidget 
COMMENT                            (CWidget 3 (10 #4# 0) 1 2 0 0 Nil -944 100) 
COMMENT                            (CObjectList 
COMMENT                             #275=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 5.18316 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #276=(CLabel (CWidget 0 (0 0) 1 2 0 0 #151# 2 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Eco}RI (1177)" "@N (@S)" 5 15.0266 
COMMENT                                   -8.71165 0 -10 4.67059 0.974079 #275#) 
COMMENT                             #277=(CScratch 
COMMENT                                   (CWidget 2 (1 #4# 2) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.14482 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #278=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #151# 109 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Eco}RI (4455)" "@N (@S)" 5 -14.4665 
COMMENT                                   -10.454 0 -10 4.92035 0.974079 #277#) 
COMMENT                             #279=(CScratch 
COMMENT                                   (CWidget 3 (1 #4# 3) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.12258 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #280=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #151# 1791863008 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Eco}RI (4479)" "@N (@S)" 5 -14.443 
COMMENT                                   -6.96932 0 -10 4.8704 0.974079 #279#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #281=(CGroupWidget 
COMMENT                            (CWidget 4 (10 #1# 0) 1 2 0 0 Nil -1363 100) 
COMMENT                            (CObjectList 
COMMENT                             #282=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 5.62622 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #283=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #151# 47402860 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (699)" "@N (@S)" 5 11.3896 
COMMENT                                   17.4233 0 -10 3.99622 0.974079 #282#) 
COMMENT                             #284=(CScratch 
COMMENT                                   (CWidget 2 (1 #1# 2) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 5.07286 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #285=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #151# 47405100 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (1296)" "@N (@S)" 5 15.6692 
COMMENT                                   -12.1963 0 -10 4.34589 0.974079 #284#) 
COMMENT                             #286=(CScratch 
COMMENT                                   (CWidget 3 (1 #1# 3) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 4.77441 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #287=(CLabel (CWidget 0 (0 0) 1 2 0 0 #151# 67 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (1618)" "@N (@S)" 5 16.7854 
COMMENT                                   -17.4233 0 -10 4.32092 0.974079 #286#) 
COMMENT                             #288=(CScratch 
COMMENT                                   (CWidget 4 (1 #1# 4) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.11145 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #289=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #151# 59604992 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Ava}I (4491)" "@N (@S)" 5 -15.7686 
COMMENT                                   -1.74233 0 -10 4.34589 0.974079 #288#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #290=(CGroupWidget 
COMMENT                            (CWidget 4 (10 #6# 0) 1 2 0 0 Nil -456 100) 
COMMENT                            (CObjectList 
COMMENT                             #291=(CScratch 
COMMENT                                   (CWidget 1 (1 #6# 1) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 5.02096 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #292=(CLabel (CWidget 0 (0 0) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (1352)" "@N (@S)" 5 16.0828 
COMMENT                                   -15.681 0 -10 4.34589 0.974079 #291#) 
COMMENT                             #293=(CScratch 
COMMENT                                   (CWidget 2 (1 #6# 2) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 4.62888 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #294=(CLabel (CWidget 0 (0 0) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (1775)" "@N (@S)" 5 16.9706 
COMMENT                                   -22.6503 0 -10 4.27096 0.974079 #293#) 
COMMENT                             #295=(CScratch 
COMMENT                                   (CWidget 3 (1 #6# 3) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 4.0125 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #296=(CLabel (CWidget 0 (0 0) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (2440)" "@N (@S)" 5 13.8968 
COMMENT                                   -26.1349 0 -10 4.47078 0.974079 #295#) 
COMMENT                             #297=(CScratch 
COMMENT                                   (CWidget 4 (1 #6# 4) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 3.06986 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #298=(CLabel (CWidget 0 (0 0) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Nco}I (3457)" "@N (@S)" 5 -4.37062 
COMMENT                                   -17.4233 0 -10 4.39585 0.974079 #297#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #299=(CGroupWidget 
COMMENT                            (CWidget 4 (10 #7# 0) 1 2 0 0 Nil -665 100) 
COMMENT                            (CObjectList 
COMMENT                             #300=(CScratch 
COMMENT                                   (CWidget 1 (1 #7# 1) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 4.70118 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #301=(CLabel (CWidget 0 (0 0) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Pst}I (1697)" "@N (@S)" 5 16.7203 
COMMENT                                   -19.1656 0 -10 4.04618 0.974079 #300#) 
COMMENT                             #302=(CScratch 
COMMENT                                   (CWidget 2 (1 #7# 2) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.87521 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #303=(CLabel (CWidget 0 (0 0) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Pst}I (3667)" "@N (@S)" 5 -6.68152 
COMMENT                                   -15.681 0 -10 4.12111 0.974079 #302#) 
COMMENT                             #304=(CScratch 
COMMENT                                   (CWidget 3 (1 #7# 3) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.14668 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #305=(CLabel (CWidget 0 (0 0) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Pst}I (4453)" "@N (@S)" 5 -14.1807 
COMMENT                                   -12.1963 0 -10 4.14608 0.974079 #304#) 
COMMENT                             #306=(CScratch 
COMMENT                                   (CWidget 4 (1 #7# 4) 1 2 0 0 #159# 0 100) 
COMMENT                                   (LOGPEN 0 6 10053171) 8 1 2.11331 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #307=(CLabel (CWidget 0 (0 0) 1 2 0 0 #151# 0 100) 
COMMENT                                   (LOGPEN 0 0 13408563) 1 
COMMENT                                   (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Georgia") 1.74233 0.555556 0 
COMMENT                                   "{\\i Pst}I (4489)" "@N (@S)" 5 -14.7905 
COMMENT                                   -5.22699 0 -10 4.19603 0.974079 #306#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #308=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -456 100) 
COMMENT                     (CObjectList) (CObjectList)) 
COMMENT               #309=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1577 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 
COMMENT       (CStringList) 0 0 
COMMENT       (CObList 
COMMENT        #310=(CSampFrag "pBlu2SKP-I-sceI" 3747 720 "HindIII" 4467 "HindIII" 
COMMENT              (LOGPEN 0 0 0) 4.86554e-316 2.00551e-280) 
COMMENT        #311=(CSampFrag "pBlu2SKP-I-sceI" 3021 4467 "HindIII" 720 "HindIII" 
COMMENT              (LOGPEN 0 0 0) 1.6976e-313 2.01481e-280))))
FEATURES             Location/Qualifiers
     primer          655..676
                     /vntifkey="27"
                     /label=T7\primer\binding\site
     misc_feature    4619..4635
                     /vntifkey="21"
                     /label=M13\R
     misc_feature    600..615
                     /vntifkey="21"
                     /label=M13\F
     promoter        656..672
                     /vntifkey="29"
                     /label=T7\promoter
     promoter        complement(4549..4568)
                     /vntifkey="29"
                     /label=T3\promoter
     misc_feature    619..648
                     /vntifkey="21"
                     /label=I-sceI
     misc_feature    4576..4605
                     /vntifkey="21"
                     /label=I-sceI
     primer          1141..1164
                     /vntifkey="27"
                     /label=E1B\forward\primer
     primer          1176..1194
                     /vntifkey="27"
                     /label=Frank-insert-forward
     misc_feature    1060..1105
                     /vntifkey="21"
                     /label=UAS-A
     misc_feature    732..777
                     /vntifkey="21"
                     /label=UAS-A
     misc_feature    1006..1059
                     /vntifkey="21"
                     /label=UAS-B
     misc_feature    778..831
                     /vntifkey="21"
                     /label=UAS-B
     misc_feature    877..930
                     /vntifkey="21"
                     /label=UAS-B
     misc_feature    832..877
                     /vntifkey="21"
                     /label=2xUAS
     misc_feature    931..1005
                     /vntifkey="21"
                     /label=2xUAS
     misc_feature    1123..1158
                     /vntifkey="21"
                     /label=E1b
     misc_feature    2441..3160
                     /vntifkey="21"
                     /label=Cerulean
     misc_feature    1259..1292
                     /vntifkey="21"
                     /label=Lox2272
     misc_feature    2388..2421
                     /vntifkey="21"
                     /label=Lox2272
     misc_feature    3411..3444
                     /vntifkey="21"
                     /label=LoxP
     misc_feature    1301..1334
                     /vntifkey="21"
                     /label=LoxP
     exon            1353..2057
                     /vntifkey="61"
                     /label=dTomato
     exon            3458..4177
                     /vntifkey="61"
                     /label=EYFP
BASE COUNT     1706 a      1851 c      1723 g      1488 t 
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 
      601 taaaacgacg gccagtgaag ttacgctagg gataacaggg taatataggc gcgcgtaata 
      661 cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcgacg gtatcgataa 
      721 gcttaggcct ccaaggcgga gtactgtcct ccgggctggc ggagtactgt cctccggcaa 
      781 ggtcggagta ctgtcctccg acactagagg tcggagtact gtcctccgac gcaaggcgga 
      841 gtactgtcct ccgggctgcg gagtactgtc ctccggcaag gtcggagtac tgtcctccga 
      901 cactagaggt cggagtactg tcctccgacg caagatcgcg tctcagcctc actttgtcgg 
      961 agtactgtcc tccgacacta gaggtcggag tactgtcctc cgacgcaagg tcggagtact 
     1021 gtcctccgac actagaggtc ggagtactgt cctccgacgc aaggcggagt actgtcctcc 
     1081 gggctggcgg agtactgtcc tccggcaagg gtcgactcta gagggtatat aatggatccc 
     1141 atcgcgtctc agcctcactt tgagctcctc cacacgaatt ccctcgacct cgaagacgcg 
     1201 tctgtttacc ggtggcgcgc aattaaccct cacctaaagg gaacaaaagc tggagctcat 
     1261 aacttcgtat aggatacttt atacgaagtt ataactcgag ataacttcgt atagcataca 
     1321 ttatacgaag ttataccggc agatctgcca ccatggtgag caagggcgag gaggtcatca 
     1381 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga 
     1441 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga 
     1501 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct 
     1561 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg 
     1621 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc 
     1681 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact 
     1741 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc 
     1801 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg 
     1861 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac 
     1921 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca 
     1981 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg 
     2041 acgagctgta caagtaaagt gtgcggccgc gactctagat cataatcagc cataccacat 
     2101 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata 
     2161 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa 
     2221 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 
     2281 tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatgc tccgagggcc 
     2341 gccaccacct gttcctgtag gaattggccg cgactctagg atctaggata acttcgtata 
     2401 ggatacttta tacgaagtta ttgatccacc ggtcgccacc atggtgagca agggcgagga 
     2461 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 
     2521 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 
     2581 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg 
     2641 gggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc 
     2701 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 
     2761 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 
     2821 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaacgccat 
     2881 cagcgacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa 
     2941 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 
     3001 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa 
     3061 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 
     3121 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatc 
     3181 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc 
     3241 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct 
     3301 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 
     3361 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaatcgag ataacttcgt 
     3421 atagcataca ttatacgaag ttataccggt cgccaccatg gtgagcaagg gcgaggagct 
     3481 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt 
     3541 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat 
     3601 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg 
     3661 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc 
     3721 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 
     3781 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 
     3841 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag 
     3901 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat 
     3961 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc 
     4021 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct 
     4081 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc 
     4141 cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgact ctagatcata 
     4201 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc 
     4261 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat 
     4321 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 
     4381 cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt 
     4441 taatgggctg caggaattcg atatcaagct tgatatcgaa ttcctgcagc ccgggggatc 
     4501 cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag 
     4561 ggttaattgc gcgctagtta cgctagggat aacagggtaa tatagtggcg taatcatggt 
     4621 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 
     4681 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 
     4741 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 
     4801 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 
     4861 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 
     4921 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 
     4981 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 
     5041 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 
     5101 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 
     5161 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 
     5221 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 
     5281 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 
     5341 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 
     5401 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 
     5461 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 
     5521 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 
     5581 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 
     5641 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 
     5701 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 
     5761 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 
     5821 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 
     5881 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 
     5941 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 
     6001 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 
     6061 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 
     6121 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 
     6181 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 
     6241 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 
     6301 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 
     6361 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 
     6421 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 
     6481 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 
     6541 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 
     6601 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 
     6661 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 
     6721 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac 
//
