LOCUS       pZH_mPP7cp_mCeru        4599 bp ds-DNA     circular     24-SEP-2018
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        189..262
                     /locus_tag="pLtetO-1"
                     /label="pLtetO-1"
                     /ApEinfo_label="pLtetO-1"
                     /ApEinfo_fwdcolor="#18ff11"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     gene            3521..4599
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    189..207
                     /locus_tag="TetO2"
                     /label="TetO2"
                     /ApEinfo_label="TetO2"
                     /ApEinfo_fwdcolor="#ff120b"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    214..232
                     /locus_tag="TetO2(1)"
                     /label="TetO2(1)"
                     /ApEinfo_label="TetO2"
                     /ApEinfo_fwdcolor="#ff120b"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      2087..2130
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYKRPAANDENYAASV*"
                     /locus_tag="rrnB_T1_terminator"
                     /label="rrnB_T1_terminator"
                     /ApEinfo_label="rrnB_T1_terminator"
                     /ApEinfo_fwdcolor="#b1a0ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      2536..3379
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /locus_tag="p15A_origin"
                     /label="p15A_origin"
                     /ApEinfo_label="p15A_origin"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -35_signal      208..213
                     /locus_tag="-33"
                     /label="-33"
                     /ApEinfo_label="-33"
                     /ApEinfo_fwdcolor="#ff4669"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     -10_signal      231..236
                     /locus_tag="-10"
                     /label="-10"
                     /ApEinfo_label="-10"
                     /ApEinfo_fwdcolor="#ff5d86"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    243..243
                     /locus_tag="pLtetO-1 +1"
                     /label="pLtetO-1 +1"
                     /ApEinfo_label="pLtetO-1 +1"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1416..2039
                     /locus_tag="TetR tn10"
                     /label="TetR tn10"
                     /ApEinfo_label="TetR tn10"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     RBS             1402..1407
                     /locus_tag="RBS"
                     /label="RBS"
                     /ApEinfo_label="RBS"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             292..657
                     /locus_tag="PP7 Coat Protein"
                     /label="PP7 Coat Protein"
                     /ApEinfo_label="PP7 Coat Protein"
                     /ApEinfo_fwdcolor="#38a9ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             673..1392
                     /locus_tag="ECFP"
                     /label="ECFP"
                     /ApEinfo_label="ECFP"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
      181 ACCATATGTC CCTATCAGTG ATAGAGATTG ACATCCCTAT CAGTGATAGA GATACTGAGC
      241 ACATCAGCAG GACGCACTGA CCgaattcat taaagaggag aaaggtaccg catgtctaaa
      301 actatcgtgc tgtctgttgg tgaagcaacc cgcaccctga ctgaaattca gtctactgct
      361 gatcgccaaa tcttcgaaga aaaagttggt ccgctggttg gccgtctgcg tctgactgct
      421 tccctgcgtc agaacggcgc gaagaccgcg tatcgcgtta atctgaaact ggatcaagcg
      481 gacgtagtgg actctggtct gccgaaagtt cgctacacgc aggtgtggtc tcacgacgtt
      541 accatcgttg ctaattccac cgaagccagc cgtaagtccc tctacgacct caccaagagc
      601 ctggttgcaa cctctcaggt tgaggatctg gtggtcaacc tggtaccact gggccgcccg
      661 ccggttgcca ccatgGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG
      721 GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC
      781 GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG
      841 CCCTGGCCCA CCCTCGTGAC CACCCTGACC TGGGGCGTGC AGTGCTTCAG CCGCTACCCC
      901 GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG
      961 CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG
     1021 GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC
     1081 ATCCTGGGGC ACAAGCTGGA GTACAACTAC ATCAGCCACA ACGTCTATAT CACCGCCGAC
     1141 AAGCAGAAGA ACGGCATCAA GGCCAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC
     1201 GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG
     1261 CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC
     1321 GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCgg catggatgaa
     1381 ctgtataagt aataatctag caggaggatt tcaccATGTC TAGATTAGAT AAAAGTAAAG
     1441 TGATTAACAG CGCATTAGAG CTGCTTAATG AGGTCGGAAT CGAAGGTTTA ACAACCCGTA
     1501 AACTCGCCCA GAAGCTAGGT GTAGAGCAGC CTACATTGTA TTGGCATGTA AAAAATAAGC
     1561 GGGCTTTGCT CGACGCCTTA GCCATTGAGA TGTTAGATAG GCACCATACT CACTTTTGCC
     1621 CTTTAGAAGG GGAAAGCTGG CAAGATTTTT TACGTAATAA CGCTAAAAGT TTTAGATGTG
     1681 CTTTACTAAG TCATCGCGAT GGAGCAAAAG TACATTTAGG TACACGGCCT ACAGAAAAAC
     1741 AGTATGAAAC TCTCGAAAAT CAATTAGCCT TTTTATGCCA ACAAGGTTTT TCACTAGAGA
     1801 ATGCATTATA TGCACTCAGC GCTGTGGGGC ATTTTACTTT AGGTTGCGTA TTGGAAGATC
     1861 AAGAGCATCA AGTCGCTAAA GAAGAAAGGG AAACACCTAC TACTGATAGT ATGCCGCCAT
     1921 TATTACGACA AGCTATCGAA TTATTTGATC ACCAAGGTGC AGAGCCAGCC TTCTTATTCG
     1981 GCCTTGAATT GATCATCTGC GGATTAGAAA AACAACTTAA ATGTGAAAGT GGGTCTTAAT
     2041 AActgcagcc cgggggatcc catggtacgc gtgctagagg catcaaataa aacgaaaggc
     2101 tcagtcgaaa gactgggcct ttcgttttat AAGCTTGGCG TAATCATGGT CATAGCTGTT
     2161 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA
     2221 GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT
     2281 GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC
     2341 GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG
     2401 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC
     2461 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG
     2521 GAACCGTAAA AAGGCgcgct agcggagtgt atactggctt actatgttgg cactgatgag
     2581 ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga
     2641 atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
     2701 gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga
     2761 agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc
     2821 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
     2881 ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt
     2941 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
     3001 tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
     3061 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa
     3121 aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc
     3181 gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta
     3241 cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt
     3301 ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt
     3361 attaatcaga taaaatattG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
     3421 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
     3481 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
     3541 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
     3601 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
     3661 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
     3721 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
     3781 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
     3841 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
     3901 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
     3961 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
     4021 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
     4081 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
     4141 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
     4201 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
     4261 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
     4321 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
     4381 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
     4441 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC
     4501 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT
     4561 TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTC  
//
