LOCUS       pAAV-CW3SL-EGFP	4476 bp 	DNA	SYN
DEFINITION  pAAV-CW3SL-EGFP
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5591
                     /dnas_title="#436-pAAV-minCamkII bPac p2A SthK mCherry
                     minWPRE"
                     /organism="pAAV-CW3SL-EGFP"
                     /mol_type="other DNA"
     rep_origin      3086..3392
                     /label=f1_origin
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    3660..3682
                     /label=pGEX_3_primer
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     promoter        3841..3869
                     /label=AmpR_promoter
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     gene            3911..4771
                     /label=Ampicillin
                     /gene="Ampicillin"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     CDS             3911..4771
                     /label=ORF frame 3
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4926..5545
                     /label=pBR322_origin
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     LTR             2854..2994
                     /note=""
     LTR             1..141
                     /note=""
     PCR_primer      complement(2476..2502)
                     /primer="CGTATCCACATAGCGTAAAAGGAGCAA"
                     /note="WPRE-Seq-R"
                     /set="Set 1"
     PCR_primer      complement(2477..2497)
                     /primer="CCACATAGCGTAAAAGGAGCA"
                     /note="pAAV_rev_f(syn)_rev"
                     /set="Set 1"
     PCR_primer      4678..4698
                     /primer="CGTAGTTATCTACACGACGGG"
                     /note="fw.out_of_Amp"
                     /set="Set 1"
     PCR_primer      5194..5214
                     /primer="TAAGTCGTGTCTTACCGGGTT"
                     /note="CB9-1412_fw"
                     /set="Set 1"
     PCR_primer      4678..4698
                     /primer="CGTAGTTATCTACACGACGGG"
                     /note="Amp904_fw"
                     /set="Set 1"
     PCR_primer      complement(3957..3975)
                     /primer="AAAACAGGAAGGCAAAATG"
                     /note="Amp4193_rev"
                     /set="Set 1"
     PCR_primer      complement(3165..3187)
                     /primer="GGCGAGAAAGGAAGGGAAGAAAG"
                     /note="CB9-4041_rev"
                     /set="Set 1"
     PCR_primer      466..486
                     /primer="GTTCTCCGTTTGCACTCAGGA"
                     /note="CamkII"
                     /set="Set 1"
     misc_feature    2400..2847
                     /note="minmal WPRE"
     misc_feature    144..557
                     /note="minimal CamkII promoter"
     PCR_primer      466..486
                     /note="Seq Primer CamKII Prom"
                     /current=0
     PCR_product     466..557
                     /note="qPCR AAV"
                     /current=0
     PCR_primer      2501..2520
                     /primer="CGCTGCTTTAATGCCTTTGT"
                     /note="WPRE 5P  fw"
                     /set="Set 1"
     PCR_primer      complement(2672..2696)
                     /primer="AGCAATAGCATCACAAATTTCACAA"
                     /note=">SV40 "
                     /set="Set 1"
     PCR_primer      complement(2721..2745)
                     /primer="AGCAATAGCATCACAAATTTCACAA"
                     /note=">SV40 "
                     /set="Set 1"
     PCR_primer      complement(2571..2591)
                     /pair="qPCR minWPRE rev"
                     /primer="CGTGGCAAGAACTAACCAGGA"
                     /current=1
     conflict        740..742
                     /note="K60E"
     misc_feature    1367..1372
                     /note="fehlt in Sequenzierung?!?"
     gene            1697..2377
                     /gene="mCherry"
                     /note="GENE GENE GENE GENE"
                     /note="GENE"
     CDS             1652..1684
                     /gene="PACa"
                     /note="CDS CDS"
                     /note="CDS"
                     /codon_start=3
     CDS             1625..1648
                     /gene="Myc (3087-3116)"
                     /note="CDS CDS CDS [pGEM-HeReg-PACa_cMyc]
                     [pGEM-HeReg-PACa_ cMyc ] [pGEM-HeReg-PACa_cMyc]"
                     /note="[pGEM-HeReg-PACa_cMyc]"
                     /codon_start=0
     PCR_primer      complement(1704..1722)
                     /primer="TTGATGACCTCCTCGCCCT"
                     /note="td_tomato_R"
                     /set="Set 1"
     PCR_primer      1694..1713
                     /primer="ATGGTGAGCAAGGGCGAGGA"
                     /note="pCAG_SeqPr2_fw"
                     /set="Set 1"
     PCR_primer      complement(1704..1723)
                     /pair="seq_5'mCherry_rev"
                     /primer="CATGTTATCCTCCTCGCCCT"
                     /current=1
     PCR_primer      complement(1704..1722)
                     /primer="TTGATGACCTCCTCGCCCT"
                     /note="td_tomato_R"
                     /set="Set 1"
     PCR_primer      1694..1713
                     /primer="ATGGTGAGCAAGGGCGAGGA"
                     /note="pCAG_SeqPr2_fw_NEU"
                     /set="Set 1"
     PCR_primer      2303..2322
                     /primer="TTGGACATCACCTCCCACAA"
                     /note="Seq"
                     /set="Set 1"
     PCR_primer      2303..2322
                     /primer="TTGGACATCACCTCCCACAA"
                     /note="Seq 3%92 mCherry fw"
                     /set="Set 1"
     PCR_primer      2110..2131
                     /primer="CGTAATGCAGAAGAAGACCATG"
                     /note="mCherry"
                     /set="Set 1"
     PCR_primer      complement(2185..2206)
                     /primer="CTTCAGCCTCTGCTTGATCTCG"
                     /note="mCherry"
                     /set="Set 1"
     PCR_primer      2378..2398
                     /primer="TACGGCATGGACGAGCTGTAC"
                     /note="td_tomato_F"
                     /set="Set 1"
     PCR_primer      2378..2398
                     /primer="TACGGCATGGACGAGCTGTAC"
                     /note="td_tomato_F"
                     /set="Set 1"
     gene            2378..2401
                     /gene="mCherry"
                     /note="GENE GENE GENE GENE"
                     /note="GENE"
     CDS             558..1612
                     /gene="huBgPAC"
                     /note="CDS CDS CDS Unknown open reading frame Unknown
                     open read ing frame Unknown open reading frame"
                     /note="Unknown open reading frame"
                     /codon_start=1
     misc_feature    558..565
                     /gene="RBS"
ORIGIN      
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCA GATGCTGCAC ACTTGTGGAC TAAGTTTGTT
      181 CGCATCCCCT TCTCCAACCC CCTCAGTACA TCACCCTGGG GGAACAGGGT CCACTTGCTC
      241 CTGGGCCCAC ACAGTCCTGC AGTATTGTGT ATATAAGGCC AGGGCAAAGA GGAGCAGGTT
      301 TTAAAGTGAA AGGCAGGCAG GTGTTGGGGA GGCAGTTACC GGGGCAACGG GAACAGGGCG
      361 TTTCGGAGGT GGTTGCCATG GGGACCTGGA TGCTGACGAA GGCTCGCGAG GCTGTGAGCA
      421 GCCACAGTGC CCTGCTCAGA AGCCCCAAGC TCGTCAGTCA AGCCGGTTCT CCGTTTGCAC
      481 TCAGGAGCAC GGGCAGGCGA GTGGCCCCTA GTTCTGGGGG CAGCGTGAAA AGACGGTATC
      541 GATAAGCTTG ATTCGAGCTA GCatgatgaa gcggctggtg tacatcagca agatcagcgg
      601 ccacctgagc ctggaagaga tccagcggat cggcaaggtg tccatcaaga acaaccagcg
      661 ggacaacatc accggcgtgc tgctgtacct gcagggcctg ttcttccaga tcctggaagg
      721 cgagaacgag aaggtggacA agctgtacaa gaaaatcctg gtggacgacc ggcacaccaa
      781 catcctgtgc ctgaaaaccg agtacgacat caccgaccgg atgttcccca actgggccat
      841 gaaaaccatc aacctgaacg agaacagcga gctgatgatc cagcccatca agagcctgct
      901 gcagaccatc acccagagcc accgggtgct ggaaaagtac atgcccgcca gagtgatcta
      961 cctgatcaac cagggcatca accccctgac cgtggagccc cagctggtcg agaagatcat
     1021 cttcttcagc gacatcctgg ccttcagcac cctgaccgag aagctgcccg tgaacgaggt
     1081 ggtcatcctg gtcaaccggt acttcagcat ctgcacccgg atcatcagcg cctacggcgg
     1141 cgaagtgacc aagttcatcg gcgactgcgt gatggccagc ttcaccaaag agcagggcga
     1201 cgccgccatc cggaccagcc tggacatcat ctccgagctg aagcagctgc ggcaccacgt
     1261 ggaggccacc aaccccctgc acctgctgta caccggcatc ggcctgagct acggccacgt
     1321 gatcgagggc aacatgggca gcagcctgaa gatggaccac accctgctgg gggacgccgt
     1381 gaacgtggcc gccaggctgg aagccctgac aagacagctg ccctacgccc tggcctttac
     1441 agccggcgtg aagaagtgct gccaggccca gtggaccttc atcaacctgg gagcccacca
     1501 ggtcaagggc aagcaggaag ccatcgaggt ctacaccgtg aatgaggccc agaagtacta
     1561 cgacaccctg cagatcaccc agctgatccg gcagaccctg gaaaacgaca agccgcggac
     1621 gtacgagcaa aagctgatct cggaggagga cctgaaggga tcgcaccacc atcaccacca
     1681 tgcagctaga tctgccgtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     1741 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     1801 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     1861 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     1921 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     1981 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     2041 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     2101 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     2161 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     2221 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     2281 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     2341 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     2401 gtaattaatt aactcgagAT AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG
     2461 GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT
     2521 ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTAG
     2581 TTCTTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC
     2641 TGTTGGGCAC TGACAATTCC GTGGTGTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT
     2701 TTGTAACCAT CTAGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT
     2761 ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG
     2821 GGGGAGATGT GGGAGGTTTT TTAAAGCGGC CGCAGGAACC CCTAGTGATG GAGTTGGCCA
     2881 CTCCCTCTCT GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC GCCCGACGCC
     2941 CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCTGCCTG CAGGGGCGCC
     3001 TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA CGTCAAAGCA
     3061 ACCATAGTAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG
     3121 CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT
     3181 TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT
     3241 CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTTGGGTG ATGGTTCACG
     3301 TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT
     3361 TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG GCTATTCTTT
     3421 TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA
     3481 AAAATTTAAC GCGAATTTTA ACAAAATATT AACGTTTACA ATTTTATGGT GCACTCTCAG
     3541 TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA
     3601 CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC
     3661 CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GACGAAAGGG
     3721 CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC
     3781 AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA
     3841 TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA
     3901 AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT
     3961 TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA
     4021 GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG
     4081 TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC
     4141 GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA
     4201 GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT
     4261 AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT
     4321 GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT
     4381 AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA
     4441 CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT
     4501 TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC
     4561 ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA
     4621 GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT
     4681 AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA
     4741 GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT
     4801 TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA
     4861 TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT
     4921 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA
     4981 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT
     5041 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA
     5101 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT
     5161 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC
     5221 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA
     5281 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA
     5341 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG
     5401 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT
     5461 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG
     5521 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT
     5581 TGCTCACATG T
//

