LOCUS pcDNA3-ExRai-CKAR(TA) 6753 bp DNA circular 5/23/18 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments= COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers CDS 922..939 /label=6His /SerialCloner_Color=&h008000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 1012..1053 /label=PKCsub_TA /SerialCloner_Color=&h999999 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 1819..2241 /label=FHA1 /SerialCloner_Color=&h804000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 1087..1809 /label=cpGFP /SerialCloner_Color=&h008000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind complement(4592..4612) /label=M13-rev /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind 863..882 /label=T7 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind complement(2306..2323) /label=SP6 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin complement(4980..5608) /label=ColE1 origin /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_binding complement(4618..4640) /label=LacO /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS complement(5760..6419) /label=AmpR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 3461..4252 /label=Kan/neoR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter complement(6659..6687) /label=Amp prom /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter complement(4645..4674) /label=lac /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 3242..3319 /label=SV40 ORI /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True terminator 2346..2573 /label=bGH PA /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True promoter 232..819 /label=CMV prom /SerialCloner_Color=&h800080 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AggatcccAT GCGATTCAGA 1021 AGATTCCAGG CGTTGAAGGA CAAGGCTAAG GCTGGCGGCA CCGGCGGCAG Cgagctcagc 1081 gcgatcAACG TCTATATCAa GGCCGACAAG CAGAAGAACG GCATCAAGGC GAACTTCAAG 1141 ATCCGCCACA ACATCGAGGA CGGCGGCGTG CAGCTCGCCT ACCACTACCA GCAGAACACC 1201 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCgt gCAGTCCAAA 1261 CTTTCGAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC 1321 GCCGGGATCA CTCTCGGCAT GGACGAGCTG TACAAGGGCG GTACCGGAGG GAGCATGGTG 1381 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC 1441 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGTGAGG GCGATGCCAC CTACGGCAAG 1501 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG 1561 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC 1621 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACATCCAGG AGCGCACCAT CTTCTTCAAG 1681 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC 1741 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG 1801 GAGTACAACa gcatgcataa gttttctcaa gaacagatcg gcgaaaacat tgtgtgcagg 1861 gtcatttgta ccacgggtca aattcccatc cgagatttgt cagctgatat ttcacaagtg 1921 cttaaggaaa aacgatccat aaagaaagtt tggacatttg gtagaaaccc agcctgtgac 1981 tatcatttag gaaacatttc aagactgtca aataagcatt tccaaatact actaggagaa 2041 gacggtaacc ttttattgaa tgacatttcc actaatggga cctggttaaa tgggcaaaaa 2101 gtcgagaaga acagcaatca gttactgtct caaggtgatg aaataaccgt tggtgtaggc 2161 gtggaatcag atattttatc tctggtcatt ttcataaacg acaaatttaa gcagtgcctc 2221 gagcagaaca aagttgatcg cTAGgaattc TGCAGATATC CATCACACTG GCGGCCGCTC 2281 GAGCATGCAT CTAGAGGGCC CTATTctata gtgtcaccta aatgctagag ctcgctgatc 2341 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 2401 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 2461 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 2521 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 2581 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 2641 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 2701 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 2761 agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 2821 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 2881 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 2941 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc 3001 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 3061 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag 3121 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 3181 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 3241 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 3301 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg 3361 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt 3421 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 3481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 3541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 3601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc 3661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 3721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 3781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 3841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 3901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 3961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca 4021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 4081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 4141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 4201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact 4261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc 4321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg 4381 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca 4441 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 4501 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata 4561 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 4621 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 4681 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 4741 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 4801 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 4861 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 4921 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 4981 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 5041 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 5101 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 5161 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg 5221 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 5281 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 5341 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 5401 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 5461 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 5521 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 5581 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 5641 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 5701 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 5761 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 5821 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 5881 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 5941 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 6001 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 6061 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 6121 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 6181 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 6241 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 6301 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 6361 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 6421 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 6481 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 6541 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 6601 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 6661 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 6721 cgcacatttc cccgaaaagt gccacctgac gtc //