LOCUS pcDNA3-ExRai-AktAR 6759 bp DNA circular 5/23/18 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments= COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers CDS 1093..1815 /label=cpGFP /SerialCloner_Color=&h008000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="direct strand translation"translation="NVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEV \ CDS 1825..2247 /label=FHA1 /SerialCloner_Color=&h804000 /SerialCloner_Show=True /SerialCloner_Protect=True /SerialCloner_Arrow=True /SerialCloner_Desc=note="direct strand translation"translation="KFSQEQIGENIVCRVICTTGQIPIRDLSADISQVLKEKRSIKKVWTFGRNPACDYHLGNISRLSNKHFQILLGEDGNLLLNDISTNGTWLNGQKVEKNSNQLLSQGDEITVGVGVESDILSLVIFINDKFKQCLEQNKVDR" \ primer_bind complement(4598..4618) /label=M13-rev /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind 863..882 /label=T7 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind complement(2312..2329) /label=SP6 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin complement(4986..5614) /label=ColE1 origin /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_binding complement(4624..4646) /label=LacO /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS complement(5766..6425) /label=AmpR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 3467..4258 /label=Kan/neoR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter complement(6665..6693) /label=Amp prom /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter complement(4651..4680) /label=lac /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 3248..3325 /label=SV40 ORI /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True terminator 2352..2579 /label=bGH PA /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True promoter 232..819 /label=CMV prom /SerialCloner_Color=&h800080 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 1015..1059 /label=AKTsub /SerialCloner_Color=&h999999 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 922..939 /label=6His /SerialCloner_Color=&h008000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AggatcccGT CGACCCTCGT 1021 CCGCGCTCGT GCACCTGGCC GGACCCCAGG CCGGAGTTTG GCGGCACCGG CGGCAGCgag 1081 ctcagcgcga tcAACGTCTA TATCAaGGCC GACAAGCAGA AGAACGGCAT CAAGGCGAAC 1141 TTCAAGATCC GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCTACCA CTACCAGCAG 1201 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCgtgCAG 1261 TCCAAACTTT CGAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG 1321 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGGGCGGTAC CGGAGGGAGC 1381 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC 1441 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GTGAGGGCGA TGCCACCTAC 1501 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC 1561 CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG 1621 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACA TCCAGGAGCG CACCATCTTC 1681 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG 1741 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC 1801 AAGCTGGAGT ACAACagcat gcataagttt tctcaagaac agatcggcga aaacattgtg 1861 tgcagggtca tttgtaccac gggtcaaatt cccatccgag atttgtcagc tgatatttca 1921 caagtgctta aggaaaaacg atccataaag aaagtttgga catttggtag aaacccagcc 1981 tgtgactatc atttaggaaa catttcaaga ctgtcaaata agcatttcca aatactacta 2041 ggagaagacg gtaacctttt attgaatgac atttccacta atgggacctg gttaaatggg 2101 caaaaagtcg agaagaacag caatcagtta ctgtctcaag gtgatgaaat aaccgttggt 2161 gtaggcgtgg aatcagatat tttatctctg gtcattttca taaacgacaa atttaagcag 2221 tgcctcgagc agaacaaagt tgatcgcTAG gaattcTGCA GATATCCATC ACACTGGCGG 2281 CCGCTCGAGC ATGCATCTAG AGGGCCCTAT Tctatagtgt cacctaaatg ctagagctcg 2341 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 2401 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 2461 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 2521 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc 2581 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg 2641 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 2701 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 2761 ccgtcaagct ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct 2821 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 2881 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 2941 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat 3001 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg 3061 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat 3121 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 3181 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 3241 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 3301 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 3361 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 3421 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 3481 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 3541 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 3601 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 3661 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 3721 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 3781 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 3841 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 3901 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 3961 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 4021 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 4081 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 4141 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 4201 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 4261 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 4321 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 4381 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt 4441 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 4501 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 4561 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 4621 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 4681 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 4741 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 4801 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 4861 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 4921 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 4981 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 5041 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 5101 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 5161 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc 5221 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 5281 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 5341 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 5401 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 5461 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 5521 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 5581 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 5641 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 5701 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 5761 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 5821 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 5881 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 5941 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 6001 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 6061 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 6121 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 6181 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 6241 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 6301 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 6361 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 6421 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 6481 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 6541 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 6601 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 6661 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 6721 gttccgcgca catttccccg aaaagtgcca cctgacgtc //