LOCUS       pcDNA3-Lyn-sapphireAKAR1    6672 bp      DNA     circular   5/23/18
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : pcDNA3-PM-CaNAR2 [5443  nt] : (EcoRI[3286] / B
            
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     sig_peptide     907..939
                     /label=Lyn-Targeting
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     promoter        232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    1006..1728
                     /label=cpTsapphire
                     /SerialCloner_Color=&h008080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(4511..4531)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(2225..2242)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     rep_origin      complement(4899..5527)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_binding    complement(4537..4559)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             complement(5679..6338)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             3380..4171
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(6578..6606)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(4564..4593)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3161..3238
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     terminator      2265..2492
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             1738..2160
                     /label=FHA1
                     /SerialCloner_Color=&h804000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    949..972
                     /label=PKAsub
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG GCTGCATCAA GAGCAAGCGC AAGGACAAGg atcccATGCT GCGTCGCGCC 
      961 ACCCTGGTGG ACGGCGGCAC CGGCGGCAGC gagctcagcg cgatcaacgt gtatatcatg 
     1021 gctgacaaac agaagaacgg tatcaaggcc aacttcaaga tccgccacaa catcgaagac 
     1081 ggcggcgttc agctggctga tcactaccag cagaacaccc caatcggcga cggccctgtt 
     1141 ctgctgcctg acaaccacta cttaagcatc cagtctaaac tgtccaaaga cccaaacgag 
     1201 aagcgcgacc acatggttct gctggaattt gttaccgccg ctggtatcac ccacggcatg 
     1261 gacgaactgt acaagggcgg caccggggga tctatggttt ccaaaggcga ggaactgttc 
     1321 actggtgttg ttcctatcct ggttgaactg gacggcgacg ttaacggcca caaattcagc 
     1381 gttagcgggg aaggcgaagg agacgctacc tacggcaagc tgactctgaa gttcatctgc 
     1441 accactggca agctgcctgt tccttggcct accctggtta ctaccttctc ctatggtgtt 
     1501 atggttttcg ccagatatcc tgaccacatg aagcagcacg acttcttcaa gagcgccatg 
     1561 cctgagggct acgttcagga acgcaccatc tttttcaagg acgacggcaa ctataagaca 
     1621 cgcgctgagg ttaagttcga gggcgacact ctggttaacc gcatcgagct gaagggcatc 
     1681 gacttcaagg aggacggcaa catcctgggc cataagcttg aatataacag catgcataag 
     1741 ttttctcaag aacagatcgg cgaaaacatt gtgtgcaggg tcatttgtac cacgggtcaa 
     1801 attcccatcc gagatttgtc agctgatatt tcacaagtgc ttaaggaaaa acgatccata 
     1861 aagaaagttt ggacatttgg tagaaaccca gcctgtgact atcatttagg aaacatttca 
     1921 agactgtcaa ataagcattt ccaaatacta ctaggagaag acggtaacct tttattgaat 
     1981 gacatttcca ctaatgggac ctggttaaat gggcaaaaag tcgagaagaa cagcaatcag 
     2041 ttactgtctc aaggtgatga aataaccgtt ggtgtaggcg tggaatcaga tattttatct 
     2101 ctggtcattt tcataaacga caaatttaag cagtgcctcg agcagaacaa agttgatcgc 
     2161 TAGgaattCT GCAGATATCC ATCACACTGG CGGCCGCTCG AGCATGCATC TAGAGGGCCC 
     2221 TATTctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag 
     2281 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 
     2341 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca 
     2401 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag 
     2461 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg 
     2521 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 
     2581 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 
     2641 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggcatccc 
     2701 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 
     2761 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 
     2821 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 
     2881 ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa aaaatgagct 
     2941 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga 
     3001 aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca attagtcagc 
     3061 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 
     3121 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 
     3181 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 
     3241 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 
     3301 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg 
     3361 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg 
     3421 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc 
     3481 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg 
     3541 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt 
     3601 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg 
     3661 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat 
     3721 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca 
     3781 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca 
     3841 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa 
     3901 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa 
     3961 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc 
     4021 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga 
     4081 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc 
     4141 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac 
     4201 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg 
     4261 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc 
     4321 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa 
     4381 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 
     4441 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc 
     4501 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 
     4561 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 
     4621 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 
     4681 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 
     4741 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 
     4801 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 
     4861 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 
     4921 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 
     4981 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 
     5041 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 
     5101 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 
     5161 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 
     5221 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 
     5281 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 
     5341 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 
     5401 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 
     5461 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 
     5521 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 
     5581 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 
     5641 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 
     5701 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 
     5761 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 
     5821 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 
     5881 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 
     5941 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 
     6001 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 
     6061 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 
     6121 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 
     6181 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 
     6241 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 
     6301 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 
     6361 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 
     6421 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 
     6481 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 
     6541 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 
     6601 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 
     6661 ccacctgacg tc
//