LOCUS       pcDNA3-sapphireCKAR    6753 bp      DNA     circular   5/23/18
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=Degen. DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    1087..1809
                     /label=cpTsapphire
                     /SerialCloner_Color=&h008080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(4592..4612)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(2306..2323)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(4980..5608)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(4618..4640)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(5760..6419)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             3461..4252
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(6659..6687)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(4645..4674)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3242..3319
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      2346..2573
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     promoter        232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1819..2241
                     /label=FHA1
                     /SerialCloner_Color=&h804000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1012..1053
                     /label=PKCsub
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             922..939
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 
      961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AggatcccAT GCGATTCAGA 
     1021 AGATTCCAGA CGTTGAAGGA CAAGGCTAAG GCTGGCGGCA CCGGCGGCAG Cgagctcagc 
     1081 gcgatcaacg tgtatatcat ggctgacaaa cagaagaacg gtatcaaggc caacttcaag 
     1141 atccgccaca acatcgaaga cggcggcgtt cagctggctg atcactacca gcagaacacc 
     1201 ccaatcggcg acggccctgt tctgctgcct gacaaccact acttaagcat ccagtctaaa 
     1261 ctgtccaaag acccaaacga gaagcgcgac cacatggttc tgctggaatt tgttaccgcc 
     1321 gctggtatca cccacggcat ggacgaactg tacaagggcg gcaccggggg atctatggtt 
     1381 tccaaaggcg aggaactgtt cactggtgtt gttcctatcc tggttgaact ggacggcgac 
     1441 gttaacggcc acaaattcag cgttagcggg gaaggcgaag gagacgctac ctacggcaag 
     1501 ctgactctga agttcatctg caccactggc aagctgcctg ttccttggcc taccctggtt 
     1561 actaccttct cctatggtgt tatggttttc gccagatatc ctgaccacat gaagcagcac 
     1621 gacttcttca agagcgccat gcctgagggc tacgttcagg aacgcaccat ctttttcaag 
     1681 gacgacggca actataagac acgcgctgag gttaagttcg agggcgacac tctggttaac 
     1741 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg ccataagctt 
     1801 gaatataaca gcatgcataa gttttctcaa gaacagatcg gcgaaaacat tgtgtgcagg 
     1861 gtcatttgta ccacgggtca aattcccatc cgagatttgt cagctgatat ttcacaagtg 
     1921 cttaaggaaa aacgatccat aaagaaagtt tggacatttg gtagaaaccc agcctgtgac 
     1981 tatcatttag gaaacatttc aagactgtca aataagcatt tccaaatact actaggagaa 
     2041 gacggtaacc ttttattgaa tgacatttcc actaatggga cctggttaaa tgggcaaaaa 
     2101 gtcgagaaga acagcaatca gttactgtct caaggtgatg aaataaccgt tggtgtaggc 
     2161 gtggaatcag atattttatc tctggtcatt ttcataaacg acaaatttaa gcagtgcctc 
     2221 gagcagaaca aagttgatcg cTAGgaattc TGCAGATATC CATCACACTG GCGGCCGCTC 
     2281 GAGCATGCAT CTAGAGGGCC CTATTctata gtgtcaccta aatgctagag ctcgctgatc 
     2341 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 
     2401 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 
     2461 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 
     2521 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 
     2581 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 
     2641 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 
     2701 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 
     2761 agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 
     2821 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 
     2881 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 
     2941 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc 
     3001 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 
     3061 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag 
     3121 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 
     3181 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 
     3241 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 
     3301 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg 
     3361 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt 
     3421 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 
     3481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 
     3541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 
     3601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc 
     3661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 
     3721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 
     3781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 
     3841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 
     3901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 
     3961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca 
     4021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 
     4081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 
     4141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 
     4201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact 
     4261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc 
     4321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg 
     4381 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca 
     4441 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 
     4501 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata 
     4561 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 
     4621 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 
     4681 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 
     4741 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 
     4801 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 
     4861 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 
     4921 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 
     4981 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 
     5041 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 
     5101 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 
     5161 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg 
     5221 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 
     5281 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 
     5341 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 
     5401 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 
     5461 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 
     5521 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 
     5581 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 
     5641 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 
     5701 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 
     5761 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 
     5821 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 
     5881 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 
     5941 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 
     6001 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 
     6061 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 
     6121 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 
     6181 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 
     6241 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 
     6301 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 
     6361 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 
     6421 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 
     6481 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 
     6541 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 
     6601 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 
     6661 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 
     6721 cgcacatttc cccgaaaagt gccacctgac gtc
//