LOCUS       pcDNA3-RAB-EKARev    7497 bp      DNA     circular   5/23/18
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             922..939
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1873..2220
                     /label=EV Linker
                     /SerialCloner_Color=&h333333
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2227..2280
                     /label=ERKsub_DD
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1705..1866
                     /label=WW_Domain
                     /SerialCloner_Color=&h804000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1009..1698
                     /label=ddRFP-A
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2287..2985
                     /label=FP-B
                     /SerialCloner_Color=&h800000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(5336..5356)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3050..3067)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5724..6352)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5362..5384)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6504..7163)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4205..4996
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(7403..7431)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5389..5418)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3986..4063
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3090..3317
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     promoter        232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 
      961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCCCAT GGTGAGCAAG 
     1021 AGCGAGGAGG TCATCAAAGA GTTCATGCGC TTCAAGGTGC GCTTGGAGGG CTCCATGAAC 
     1081 GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC 
     1141 GCCAAGCTGA AGGTGACCAA GGGCGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCC 
     1201 CAGATCATGT ACGGCTCCAA GGCGTACGTG AAGCACCCCG CCGACGTCCC CGATTACATG 
     1261 AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGCACTTCGA GGACGGCGGT 
     1321 CTGGTGACCG TGACTCAGGA CACCTCCCTG CAGGACGGCA CGCTGATCTA CAAGGTGAAG 
     1381 ATGCGCGGCA CCAACTTCCC CCCCGACGGC CCCGTAATGC AGAAGAAGAC CTTGGGCTGG 
     1441 GATTATGCCA CCGAGCGCCT GTACCCCGAA GACGGCGTGC TGAAGGGCGA GCTTCTGGGG 
     1501 CGCCTGAAGC TGAAGGACGG CGGCCTCAAC CTGGTGGAGT TCAAGACCAT CTACATGGCC 
     1561 AAGAAGCCCG TGCAACTGCC CGGCTACTAC TTCGTGGACA CCAAGCTGGG CATCACCTCC 
     1621 CACAACGAGG ACTACACCAT CGTGGAACAG TACGAGCGCT CCGAGGGCCG CCACCACCTG 
     1681 GGGCATGGCA CCGGCAGCAG ATCTATGGCG GACGAGGAGA AGCTGCCGCC CGGCTGGGAG 
     1741 AAGCGCATGA GCCGCAGCTC AGGCCGAGTG TACTACTTCA ACCACATCAC TAACGCCAGC 
     1801 CAGTGGGAGC GGCCCAGCGG CAACAGCAGC AGTGGTGGCA AAAACGGGCA GGGGGAGCCT 
     1861 GCCAGGGGTA CCAGTGCTGG TGGTAGTGCT GGTGGTAGTG CTGGTGGTAG TGCTGGTGGT 
     1921 AGTGCTGGTG GTtccggcAG TGCTGGTGGT AGTGCTGGTG GTagtaccAG TGCTGGTGGT 
     1981 AGTGCTGGTG GTAGTGCTGG TGGTAGTGCT GGTGGTAGTG CTGGTGGTtc cggcAGTGCT 
     2041 GGTGGTAGTG CTGGTGGTag taccAGTGCT GGTGGTAGTG CTGGTGGTAG TGCTGGTGGT 
     2101 AGTGCTGGTG GTAGTGCTGG TGGTtccggc AGTGCTGGTG GTAGTGCTGG TGGTagtacc 
     2161 AGTGCTGGTG GTAGTGCTGG TGGTAGTGCT GGTGGTAGTG CTGGTGGTAG TGCTGGTGGT 
     2221 tccggaCCAG ATGTCCCTAG AACTCCAGTG GATAAAgcaa agctgtcaTT CCAATTTCCG 
     2281 GGTACCGCCT CCTCCGAGGA CAACAACATG GCCACCATCA AAGAGTTCAT GCGCTTCAAG 
     2341 GTGCGCATGG AGGGCTCCAT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC 
     2401 CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGCGG CCCCCTGCCC 
     2461 TTCGCCTGGG ACATCCTGTC CCCCCAGTTC ATGTACGGCT CCGAGGCGTA CGTGAGGCAC 
     2521 CCCGCCGACA TCCCCGATTA CAAGAAGCTG CCCTTCCCCG AGGGCTTCAA GTGGGAGCGC 
     2581 GTGATGAACT TCGAAGACGG CGGTCTGGTG ACCGTTACCC AGGACTCCTC CCTGCAGGAC 
     2641 GGCACGCTGA TCTGCAAGGT GAAGATGCGC GGCACCAACT TCCCCCCCGA CGGCCCCGTA 
     2701 ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCACCGAGA TGCTGTACCC CGAAGACGGC 
     2761 GTGCTGAAGG GCCATAGCTA TCAGGCCCTG AAGCTGAAGG ACGGCGGCCA CTACCTGGTG 
     2821 GAGTTCGAGA CCATCTACAT GGCCAAGAAG CCCGTGCAAC TGCCCGGCGA TTACTGTGTG 
     2881 GACACCAAGC TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGGG 
     2941 CGCTCCGAGG GCCGCCACCG TCTGGGCATG GACGAGCGGT ACAAGTAAGA ATTCTGCAGA 
     3001 TATCCATCAC ACTGGCGGCC GCTCGAGCAT GCATCTAGAG GGCCCTATTc tatagtgtca 
     3061 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt 
     3121 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc 
     3181 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg 
     3241 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga 
     3301 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc 
     3361 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 
     3421 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 
     3481 cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt 
     3541 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 
     3601 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 
     3661 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 
     3721 ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 
     3781 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc 
     3841 ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 
     3901 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
     3961 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 
     4021 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 
     4081 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 
     4141 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg 
     4201 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 
     4261 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 
     4321 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 
     4381 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 
     4441 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 
     4501 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 
     4561 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 
     4621 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 
     4681 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 
     4741 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 
     4801 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 
     4861 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 
     4921 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 
     4981 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 
     5041 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 
     5101 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 
     5161 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 
     5221 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 
     5281 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 
     5341 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 
     5401 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 
     5461 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 
     5521 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
     5581 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     5641 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     5701 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     5761 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     5821 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     5881 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc 
     5941 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     6001 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     6061 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     6121 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     6181 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     6241 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     6301 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     6361 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 
     6421 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     6481 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     6541 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 
     6601 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 
     6661 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 
     6721 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 
     6781 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 
     6841 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 
     6901 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 
     6961 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 
     7021 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 
     7081 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 
     7141 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 
     7201 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 
     7261 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 
     7321 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 
     7381 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 
     7441 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//