LOCUS pcDNA3-RAB-EKARev(TA) 7497 bp DNA circular 5/23/18 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT Serial Cloner Genbank Format COMMENT SerialCloner_Type=DNA COMMENT SerialCloner_Comments= COMMENT SerialCloner_Ends=0,0,,0, FEATURES Location/Qualifiers CDS 922..939 /label=6His /SerialCloner_Color=&h008000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 1873..2220 /label=EV Linker /SerialCloner_Color=&h333333 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 2227..2280 /label=ERKsub_TA /SerialCloner_Color=&h999999 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 1705..1866 /label=WW_Domain /SerialCloner_Color=&h804000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 1009..1698 /label=ddRFP-A /SerialCloner_Color=&hFF0000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 2287..2985 /label=FP-B /SerialCloner_Color=&h800000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind complement(5336..5356) /label=M13-rev /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind 863..882 /label=T7 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True primer_bind complement(3050..3067) /label=SP6 /SerialCloner_Color=&h008B8B /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True rep_origin complement(5724..6352) /label=ColE1 origin /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_binding complement(5362..5384) /label=LacO /SerialCloner_Color=&h6495ED /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS complement(6504..7163) /label=AmpR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True CDS 4205..4996 /label=Kan/neoR /SerialCloner_Color=&h8FBC8F /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter complement(7403..7431) /label=Amp prom /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True Promoter complement(5389..5418) /label=lac /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True misc_feature 3986..4063 /label=SV40 ORI /SerialCloner_Color=&h4682B4 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True terminator 3090..3317 /label=bGH PA /SerialCloner_Color=&hFF8000 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True promoter 232..819 /label=CMV prom /SerialCloner_Color=&h800080 /SerialCloner_Show=True /SerialCloner_Protect=False /SerialCloner_Arrow=True ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCCCAT GGTGAGCAAG 1021 AGCGAGGAGG TCATCAAAGA GTTCATGCGC TTCAAGGTGC GCTTGGAGGG CTCCATGAAC 1081 GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC 1141 GCCAAGCTGA AGGTGACCAA GGGCGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCC 1201 CAGATCATGT ACGGCTCCAA GGCGTACGTG AAGCACCCCG CCGACGTCCC CGATTACATG 1261 AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGCACTTCGA GGACGGCGGT 1321 CTGGTGACCG TGACTCAGGA CACCTCCCTG CAGGACGGCA CGCTGATCTA CAAGGTGAAG 1381 ATGCGCGGCA CCAACTTCCC CCCCGACGGC CCCGTAATGC AGAAGAAGAC CTTGGGCTGG 1441 GATTATGCCA CCGAGCGCCT GTACCCCGAA GACGGCGTGC TGAAGGGCGA GCTTCTGGGG 1501 CGCCTGAAGC TGAAGGACGG CGGCCTCAAC CTGGTGGAGT TCAAGACCAT CTACATGGCC 1561 AAGAAGCCCG TGCAACTGCC CGGCTACTAC TTCGTGGACA CCAAGCTGGG CATCACCTCC 1621 CACAACGAGG ACTACACCAT CGTGGAACAG TACGAGCGCT CCGAGGGCCG CCACCACCTG 1681 GGGCATGGCA CCGGCAGCAG ATCTATGGCG GACGAGGAGA AGCTGCCGCC CGGCTGGGAG 1741 AAGCGCATGA GCCGCAGCTC AGGCCGAGTG TACTACTTCA ACCACATCAC TAACGCCAGC 1801 CAGTGGGAGC GGCCCAGCGG CAACAGCAGC AGTGGTGGCA AAAACGGGCA GGGGGAGCCT 1861 GCCAGGGGTA CCAGTGCTGG TGGTAGTGCT GGTGGTAGTG CTGGTGGTAG TGCTGGTGGT 1921 AGTGCTGGTG GTtccggcAG TGCTGGTGGT AGTGCTGGTG GTagtaccAG TGCTGGTGGT 1981 AGTGCTGGTG GTAGTGCTGG TGGTAGTGCT GGTGGTAGTG CTGGTGGTtc cggcAGTGCT 2041 GGTGGTAGTG CTGGTGGTag taccAGTGCT GGTGGTAGTG CTGGTGGTAG TGCTGGTGGT 2101 AGTGCTGGTG GTAGTGCTGG TGGTtccggc AGTGCTGGTG GTAGTGCTGG TGGTagtacc 2161 AGTGCTGGTG GTAGTGCTGG TGGTAGTGCT GGTGGTAGTG CTGGTGGTAG TGCTGGTGGT 2221 tccggaCCAG ATGTCCCTAG AGCTCCAGTG GATAAAGCAA AGCTGTCATT CCAATTTCCG 2281 GGTACCGCCT CCTCCGAGGA CAACAACATG GCCACCATCA AAGAGTTCAT GCGCTTCAAG 2341 GTGCGCATGG AGGGCTCCAT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC 2401 CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGCGG CCCCCTGCCC 2461 TTCGCCTGGG ACATCCTGTC CCCCCAGTTC ATGTACGGCT CCGAGGCGTA CGTGAGGCAC 2521 CCCGCCGACA TCCCCGATTA CAAGAAGCTG CCCTTCCCCG AGGGCTTCAA GTGGGAGCGC 2581 GTGATGAACT TCGAAGACGG CGGTCTGGTG ACCGTTACCC AGGACTCCTC CCTGCAGGAC 2641 GGCACGCTGA TCTGCAAGGT GAAGATGCGC GGCACCAACT TCCCCCCCGA CGGCCCCGTA 2701 ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCACCGAGA TGCTGTACCC CGAAGACGGC 2761 GTGCTGAAGG GCCATAGCTA TCAGGCCCTG AAGCTGAAGG ACGGCGGCCA CTACCTGGTG 2821 GAGTTCGAGA CCATCTACAT GGCCAAGAAG CCCGTGCAAC TGCCCGGCGA TTACTGTGTG 2881 GACACCAAGC TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGGG 2941 CGCTCCGAGG GCCGCCACCG TCTGGGCATG GACGAGCGGT ACAAGTAAGA ATTCTGCAGA 3001 TATCCATCAC ACTGGCGGCC GCTCGAGCAT GCATCTAGAG GGCCCTATTc tatagtgtca 3061 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt 3121 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc 3181 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg 3241 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga 3301 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc 3361 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 3421 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 3481 cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt 3541 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 3601 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 3661 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 3721 ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 3781 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc 3841 ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 3901 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 3961 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 4021 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 4081 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 4141 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg 4201 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 4261 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 4321 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 4381 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 4441 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 4501 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 4561 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 4621 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 4681 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 4741 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 4801 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 4861 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 4921 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 4981 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 5041 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 5101 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 5161 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 5221 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 5281 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 5341 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 5401 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 5461 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 5521 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 5581 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 5641 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 5701 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 5761 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 5821 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 5881 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc 5941 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 6001 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 6061 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 6121 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 6181 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 6241 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 6301 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 6361 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 6421 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 6481 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 6541 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 6601 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 6661 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 6721 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 6781 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 6841 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 6901 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 6961 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 7021 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 7081 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 7141 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 7201 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 7261 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 7321 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 7381 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 7441 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc //