LOCUS       pcDNA3-RAB-EKARev-NLS    7488 bp      DNA     circular   5/23/18
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=Degen. DNA
COMMENT     SerialCloner_Comments=Ligation of : pcDNA3-AKAR4-NLS.xdna [5500  nt] : (EcoRI[2983
            ] / BamHI[1002]) to pcDNA3-RAB-EKARev [1983  nt] : (BamHI[10
            02] / EcoRI[2989])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    2992..3021
                     /label=NLS-Targeting
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             922..939
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1873..2220
                     /label=EV Linker
                     /SerialCloner_Color=&h333333
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2227..2280
                     /label=ERKsub_DD
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1705..1866
                     /label=WW_Domain
                     /SerialCloner_Color=&h804000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1009..1698
                     /label=ddRFP-A
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2287..2985
                     /label=FP-B
                     /SerialCloner_Color=&h800000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(5327..5347)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3041..3058)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5715..6343)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5353..5375)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6495..7154)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4196..4987
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(7394..7422)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5380..5409)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3977..4054
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3081..3308
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     promoter        232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 
      961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGgatcCCAT GGTGAGCAAG 
     1021 AGCGAGGAGG TCATCAAAGA GTTCATGCGC TTCAAGGTGC GCTTGGAGGG CTCCATGAAC 
     1081 GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC 
     1141 GCCAAGCTGA AGGTGACCAA GGGCGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCC 
     1201 CAGATCATGT ACGGCTCCAA GGCGTACGTG AAGCACCCCG CCGACGTCCC CGATTACATG 
     1261 AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGCACTTCGA GGACGGCGGT 
     1321 CTGGTGACCG TGACTCAGGA CACCTCCCTG CAGGACGGCA CGCTGATCTA CAAGGTGAAG 
     1381 ATGCGCGGCA CCAACTTCCC CCCCGACGGC CCCGTAATGC AGAAGAAGAC CTTGGGCTGG 
     1441 GATTATGCCA CCGAGCGCCT GTACCCCGAA GACGGCGTGC TGAAGGGCGA GCTTCTGGGG 
     1501 CGCCTGAAGC TGAAGGACGG CGGCCTCAAC CTGGTGGAGT TCAAGACCAT CTACATGGCC 
     1561 AAGAAGCCCG TGCAACTGCC CGGCTACTAC TTCGTGGACA CCAAGCTGGG CATCACCTCC 
     1621 CACAACGAGG ACTACACCAT CGTGGAACAG TACGAGCGCT CCGAGGGCCG CCACCACCTG 
     1681 GGGCATGGCA CCGGCAGCAG ATCTATGGCG GACGAGGAGA AGCTGCCGCC CGGCTGGGAG 
     1741 AAGCGCATGA GCCGCAGCTC AGGCCGAGTG TACTACTTCA ACCACATCAC TAACGCCAGC 
     1801 CAGTGGGAGC GGCCCAGCGG CAACAGCAGC AGTGGTGGCA AAAACGGGCA GGGGGAGCCT 
     1861 GCCAGGGGTA CCAGTGCTGG TGGTAGTGCT GGTGGTAGTG CTGGTGGTAG TGCTGGTGGT 
     1921 AGTGCTGGTG GTtccggcAG TGCTGGTGGT AGTGCTGGTG GTagtaccAG TGCTGGTGGT 
     1981 AGTGCTGGTG GTAGTGCTGG TGGTAGTGCT GGTGGTAGTG CTGGTGGTtc cggcAGTGCT 
     2041 GGTGGTAGTG CTGGTGGTag taccAGTGCT GGTGGTAGTG CTGGTGGTAG TGCTGGTGGT 
     2101 AGTGCTGGTG GTAGTGCTGG TGGTtccggc AGTGCTGGTG GTAGTGCTGG TGGTagtacc 
     2161 AGTGCTGGTG GTAGTGCTGG TGGTAGTGCT GGTGGTAGTG CTGGTGGTAG TGCTGGTGGT 
     2221 tccggaCCAG ATGTCCCTAG AACTCCAGTG GATAAAgcaa agctgtcaTT CCAATTTCCG 
     2281 GGTACCGCCT CCTCCGAGGA CAACAACATG GCCACCATCA AAGAGTTCAT GCGCTTCAAG 
     2341 GTGCGCATGG AGGGCTCCAT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC 
     2401 CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGCGG CCCCCTGCCC 
     2461 TTCGCCTGGG ACATCCTGTC CCCCCAGTTC ATGTACGGCT CCGAGGCGTA CGTGAGGCAC 
     2521 CCCGCCGACA TCCCCGATTA CAAGAAGCTG CCCTTCCCCG AGGGCTTCAA GTGGGAGCGC 
     2581 GTGATGAACT TCGAAGACGG CGGTCTGGTG ACCGTTACCC AGGACTCCTC CCTGCAGGAC 
     2641 GGCACGCTGA TCTGCAAGGT GAAGATGCGC GGCACCAACT TCCCCCCCGA CGGCCCCGTA 
     2701 ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCACCGAGA TGCTGTACCC CGAAGACGGC 
     2761 GTGCTGAAGG GCCATAGCTA TCAGGCCCTG AAGCTGAAGG ACGGCGGCCA CTACCTGGTG 
     2821 GAGTTCGAGA CCATCTACAT GGCCAAGAAG CCCGTGCAAC TGCCCGGCGA TTACTGTGTG 
     2881 GACACCAAGC TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGGG 
     2941 CGCTCCGAGG GCCGCCACCG TCTGGGCATG GACGAGCGGT ACAAGGaatt CCCCAAAAAG 
     3001 AAGAGAAAGG TGGAAGACGC ATAATCTAGA GGGCCCTATT ctatagtgtc acctaaatgc 
     3061 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc 
     3121 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 
     3181 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 
     3241 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 
     3301 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc 
     3361 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 
     3421 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 
     3481 cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt 
     3541 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 
     3601 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 
     3661 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 
     3721 tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 
     3781 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg 
     3841 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 
     3901 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 
     3961 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 
     4021 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 
     4081 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 
     4141 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga 
     4201 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 
     4261 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 
     4321 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga 
     4381 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 
     4441 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 
     4501 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 
     4561 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 
     4621 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 
     4681 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga 
     4741 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 
     4801 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 
     4861 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 
     4921 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 
     4981 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 
     5041 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 
     5101 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 
     5161 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 
     5221 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 
     5281 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 
     5341 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 
     5401 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 
     5461 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 
     5521 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 
     5581 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 
     5641 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 
     5701 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 
     5761 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 
     5821 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 
     5881 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt 
     5941 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 
     6001 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 
     6061 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 
     6121 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 
     6181 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 
     6241 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 
     6301 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 
     6361 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 
     6421 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 
     6481 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 
     6541 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 
     6601 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 
     6661 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 
     6721 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 
     6781 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 
     6841 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 
     6901 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 
     6961 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 
     7021 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 
     7081 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 
     7141 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 
     7201 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 
     7261 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 
     7321 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 
     7381 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 
     7441 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//