LOCUS       pcDNA3-RAB-AKARev    7737 bp      DNA     circular   5/23/18
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             1009..1698
                     /label=ddRFP-A
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2527..3225
                     /label=FP-B
                     /SerialCloner_Color=&h800000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(5576..5596)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3290..3307)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5964..6592)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5602..5624)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6744..7403)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4445..5236
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(7643..7671)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5629..5658)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4226..4303
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3330..3557
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     promoter        232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2134..2481
                     /label=EV Linker
                     /SerialCloner_Color=&h333333
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1705..2127
                     /label=FHA1
                     /SerialCloner_Color=&h804000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2488..2511
                     /label=PKAsub
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             922..939
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 
      961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCCCAT GGTGAGCAAG 
     1021 AGCGAGGAGG TCATCAAAGA GTTCATGCGC TTCAAGGTGC GCTTGGAGGG CTCCATGAAC 
     1081 GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC 
     1141 GCCAAGCTGA AGGTGACCAA GGGCGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCC 
     1201 CAGATCATGT ACGGCTCCAA GGCGTACGTG AAGCACCCCG CCGACGTCCC CGATTACATG 
     1261 AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGCACTTCGA GGACGGCGGT 
     1321 CTGGTGACCG TGACTCAGGA CACCTCCCTG CAGGACGGCA CGCTGATCTA CAAGGTGAAG 
     1381 ATGCGCGGCA CCAACTTCCC CCCCGACGGC CCCGTAATGC AGAAGAAGAC CTTGGGCTGG 
     1441 GATTATGCCA CCGAGCGCCT GTACCCCGAA GACGGCGTGC TGAAGGGCGA GCTTCTGGGG 
     1501 CGCCTGAAGC TGAAGGACGG CGGCCTCAAC CTGGTGGAGT TCAAGACCAT CTACATGGCC 
     1561 AAGAAGCCCG TGCAACTGCC CGGCTACTAC TTCGTGGACA CCAAGCTGGG CATCACCTCC 
     1621 CACAACGAGG ACTACACCAT CGTGGAACAG TACGAGCGCT CCGAGGGCCG CCACCACCTG 
     1681 GGGCATGGCA CCGGCAGCAG ATCTaagttt tctcaagaac agatcggcga aaacattgtg 
     1741 tgcagggtca tttgtaccac gggtcaaatt cccatccgag atttgtcagc tgatatttca 
     1801 caagtgctta aggaaaaacg atccataaag aaagtttgga catttggtag aaacccagcc 
     1861 tgtgactatc atttaggaaa catttcaaga ctgtcaaata agcatttcca aatactacta 
     1921 ggagaagacg gtaacctttt attgaatgac atttccacta atgggacctg gttaaatggg 
     1981 caaaaagtcg agaagaacag caatcagtta ctgtctcaag gtgatgaaat aaccgttggt 
     2041 gtaggcgtgg aatcagatat tttatctctg gtcattttca taaacgacaa atttaagcag 
     2101 tgcctcgagc agaacaaagt tgatcgcGGT ACCAGTGCTG GTGGTAGTGC TGGTGGTAGT 
     2161 GCTGGTGGTA GTGCTGGTGG TAGTGCTGGT GGTtccggcA GTGCTGGTGG TAGTGCTGGT 
     2221 GGTagtaccA GTGCTGGTGG TAGTGCTGGT GGTAGTGCTG GTGGTAGTGC TGGTGGTAGT 
     2281 GCTGGTGGTt ccggcAGTGC TGGTGGTAGT GCTGGTGGTa gtaccAGTGC TGGTGGTAGT 
     2341 GCTGGTGGTA GTGCTGGTGG TAGTGCTGGT GGTAGTGCTG GTGGTtccgg cAGTGCTGGT 
     2401 GGTAGTGCTG GTGGTagtac cAGTGCTGGT GGTAGTGCTG GTGGTAGTGC TGGTGGTAGT 
     2461 GCTGGTGGTA GTGCTGGTGG TTCCGGATTG AGGCGCGCGA CGCTGGTTGA CGGCGGCAGC 
     2521 GAGCTCGCCT CCTCCGAGGA CAACAACATG GCCACCATCA AAGAGTTCAT GCGCTTCAAG 
     2581 GTGCGCATGG AGGGCTCCAT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA GGGCGAGGGC 
     2641 CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGCGG CCCCCTGCCC 
     2701 TTCGCCTGGG ACATCCTGTC CCCCCAGTTC ATGTACGGCT CCGAGGCGTA CGTGAGGCAC 
     2761 CCCGCCGACA TCCCCGATTA CAAGAAGCTG CCCTTCCCCG AGGGCTTCAA GTGGGAGCGC 
     2821 GTGATGAACT TCGAAGACGG CGGTCTGGTG ACCGTTACCC AGGACTCCTC CCTGCAGGAC 
     2881 GGCACGCTGA TCTGCAAGGT GAAGATGCGC GGCACCAACT TCCCCCCCGA CGGCCCCGTA 
     2941 ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCACCGAGA TGCTGTACCC CGAAGACGGC 
     3001 GTGCTGAAGG GCCATAGCTA TCAGGCCCTG AAGCTGAAGG ACGGCGGCCA CTACCTGGTG 
     3061 GAGTTCGAGA CCATCTACAT GGCCAAGAAG CCCGTGCAAC TGCCCGGCGA TTACTGTGTG 
     3121 GACACCAAGC TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA ACAGTACGGG 
     3181 CGCTCCGAGG GCCGCCACCG TCTGGGCATG GACGAGCGGT ACAAGTAAGA ATTCTGCAGA 
     3241 TATCCATCAC ACTGGCGGCC GCTCGAGCAT GCATCTAGAG GGCCCTATTc tatagtgtca 
     3301 cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt 
     3361 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc 
     3421 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg 
     3481 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga 
     3541 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc 
     3601 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 
     3661 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 
     3721 cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt 
     3781 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 
     3841 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 
     3901 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 
     3961 ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 
     4021 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc 
     4081 ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 
     4141 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
     4201 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 
     4261 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 
     4321 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 
     4381 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg 
     4441 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 
     4501 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 
     4561 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 
     4621 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 
     4681 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 
     4741 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 
     4801 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 
     4861 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 
     4921 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 
     4981 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 
     5041 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 
     5101 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 
     5161 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 
     5221 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 
     5281 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 
     5341 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 
     5401 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 
     5461 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 
     5521 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 
     5581 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 
     5641 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 
     5701 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 
     5761 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
     5821 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     5881 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     5941 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     6001 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     6061 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     6121 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc 
     6181 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     6241 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     6301 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     6361 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     6421 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     6481 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     6541 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     6601 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 
     6661 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     6721 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     6781 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 
     6841 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 
     6901 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 
     6961 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 
     7021 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 
     7081 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 
     7141 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 
     7201 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 
     7261 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 
     7321 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 
     7381 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 
     7441 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 
     7501 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 
     7561 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 
     7621 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 
     7681 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//