LOCUS       pcDNA3-RAB-AKARev(TA)    7449 bp      DNA     circular   5/23/18
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             922..939
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2200..2223
                     /label=PKAsub_TA
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1705..2127
                     /label=FHA1
                     /SerialCloner_Color=&h804000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1009..1698
                     /label=ddRFP-A
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2239..2937
                     /label=FP-B
                     /SerialCloner_Color=&h800000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(5288..5308)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3002..3019)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5676..6304)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5314..5336)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6456..7115)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4157..4948
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(7355..7383)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5341..5370)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3938..4015
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3042..3269
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     promoter        232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 
      961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCCCAT GGTGAGCAAG 
     1021 AGCGAGGAGG TCATCAAAGA GTTCATGCGC TTCAAGGTGC GCTTGGAGGG CTCCATGAAC 
     1081 GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC 
     1141 GCCAAGCTGA AGGTGACCAA GGGCGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCC 
     1201 CAGATCATGT ACGGCTCCAA GGCGTACGTG AAGCACCCCG CCGACGTCCC CGATTACATG 
     1261 AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGCACTTCGA GGACGGCGGT 
     1321 CTGGTGACCG TGACTCAGGA CACCTCCCTG CAGGACGGCA CGCTGATCTA CAAGGTGAAG 
     1381 ATGCGCGGCA CCAACTTCCC CCCCGACGGC CCCGTAATGC AGAAGAAGAC CTTGGGCTGG 
     1441 GATTATGCCA CCGAGCGCCT GTACCCCGAA GACGGCGTGC TGAAGGGCGA GCTTCTGGGG 
     1501 CGCCTGAAGC TGAAGGACGG CGGCCTCAAC CTGGTGGAGT TCAAGACCAT CTACATGGCC 
     1561 AAGAAGCCCG TGCAACTGCC CGGCTACTAC TTCGTGGACA CCAAGCTGGG CATCACCTCC 
     1621 CACAACGAGG ACTACACCAT CGTGGAACAG TACGAGCGCT CCGAGGGCCG CCACCACCTG 
     1681 GGGCATGGCA CCGGCAGCAG ATCTaagttt tctcaagaac agatcggcga aaacattgtg 
     1741 tgcagggtca tttgtaccac gggtcaaatt cccatccgag atttgtcagc tgatatttca 
     1801 caagtgctta aggaaaaacg atccataaag aaagtttgga catttggtag aaacccagcc 
     1861 tgtgactatc atttaggaaa catttcaaga ctgtcaaata agcatttcca aatactacta 
     1921 ggagaagacg gtaacctttt attgaatgac atttccacta atgggacctg gttaaatggg 
     1981 caaaaagtcg agaagaacag caatcagtta ctgtctcaag gtgatgaaat aaccgttggt 
     2041 gtaggcgtgg aatcagatat tttatctctg gtcattttca taaacgacaa atttaagcag 
     2101 tgcctcgagc agaacaaagt tgatcgcGGT ACCAGTGCTG GTGGTAGTGC TGGTGGTAGT 
     2161 GCTGGTGGTA GTGCTGGTGG TAGTGCTGGT GGTTCCGGAT TGAGGCGCGC GGCCCTGGTT 
     2221 GACGGCGGCA GCGAGCTCGC CTCCTCCGAG GACAACAACA TGGCCACCAT CAAAGAGTTC 
     2281 ATGCGCTTCA AGGTGCGCAT GGAGGGCTCC ATGAACGGCC ACGAGTTCGA GATCGAGGGC 
     2341 GAGGGCGAGG GCCGCCCCTA CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGC 
     2401 GGCCCCCTGC CCTTCGCCTG GGACATCCTG TCCCCCCAGT TCATGTACGG CTCCGAGGCG 
     2461 TACGTGAGGC ACCCCGCCGA CATCCCCGAT TACAAGAAGC TGCCCTTCCC CGAGGGCTTC 
     2521 AAGTGGGAGC GCGTGATGAA CTTCGAAGAC GGCGGTCTGG TGACCGTTAC CCAGGACTCC 
     2581 TCCCTGCAGG ACGGCACGCT GATCTGCAAG GTGAAGATGC GCGGCACCAA CTTCCCCCCC 
     2641 GACGGCCCCG TAATGCAGAA GAAGACCATG GGCTGGGAGG CCTCCACCGA GATGCTGTAC 
     2701 CCCGAAGACG GCGTGCTGAA GGGCCATAGC TATCAGGCCC TGAAGCTGAA GGACGGCGGC 
     2761 CACTACCTGG TGGAGTTCGA GACCATCTAC ATGGCCAAGA AGCCCGTGCA ACTGCCCGGC 
     2821 GATTACTGTG TGGACACCAA GCTGGACATC ACCTCCCACA ACGAGGACTA CACCATCGTG 
     2881 GAACAGTACG GGCGCTCCGA GGGCCGCCAC CGTCTGGGCA TGGACGAGCG GTACAAGTAA 
     2941 GAATTCTGCA GATATCCATC ACACTGGCGG CCGCTCGAGC ATGCATCTAG AGGGCCCTAT 
     3001 Tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg 
     3061 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 
     3121 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 
     3181 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 
     3241 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc 
     3301 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 
     3361 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 
     3421 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt 
     3481 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 
     3541 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 
     3601 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 
     3661 ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat 
     3721 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag 
     3781 tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
     3841 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 
     3901 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 
     3961 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 
     4021 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 
     4081 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg 
     4141 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 
     4201 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 
     4261 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 
     4321 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 
     4381 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 
     4441 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 
     4501 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 
     4561 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 
     4621 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 
     4681 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 
     4741 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 
     4801 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 
     4861 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 
     4921 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa 
     4981 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg 
     5041 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 
     5101 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc 
     5161 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 
     5221 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg 
     5281 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 
     5341 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 
     5401 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 
     5461 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 
     5521 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 
     5581 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 
     5641 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 
     5701 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 
     5761 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 
     5821 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 
     5881 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 
     5941 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 
     6001 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 
     6061 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 
     6121 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 
     6181 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 
     6241 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 
     6301 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 
     6361 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 
     6421 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 
     6481 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 
     6541 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 
     6601 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 
     6661 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 
     6721 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 
     6781 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 
     6841 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 
     6901 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 
     6961 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 
     7021 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 
     7081 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 
     7141 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 
     7201 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 
     7261 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 
     7321 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 
     7381 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 
     7441 cctgacgtc
//